##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140015_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10093901
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69202362892206	34.0	31.0	34.0	31.0	34.0
2	32.880848345946724	34.0	31.0	34.0	31.0	34.0
3	32.976338186792205	34.0	31.0	34.0	31.0	34.0
4	36.381262011585015	37.0	37.0	37.0	35.0	37.0
5	36.214714212077176	37.0	37.0	37.0	35.0	37.0
6	36.26513376741064	37.0	37.0	37.0	35.0	37.0
7	36.266231955316385	37.0	37.0	37.0	35.0	37.0
8	36.25138596069052	37.0	37.0	37.0	35.0	37.0
9	38.1484242811575	39.0	39.0	39.0	37.0	39.0
10-11	38.03823794190175	39.0	38.0	39.0	36.0	39.0
12-13	38.07847877644134	39.0	38.0	39.0	37.0	39.0
14-15	39.50155564236265	41.0	39.0	41.0	37.0	41.0
16-17	39.48515261839798	40.5	39.0	41.0	37.0	41.0
18-19	39.531495008718636	41.0	39.0	41.0	37.0	41.0
20-21	39.54692962537603	41.0	39.0	41.0	37.0	41.0
22-23	39.4840117573628	41.0	39.0	41.0	37.0	41.0
24-25	39.44433084347803	41.0	39.0	41.0	37.0	41.0
26-27	39.40669509727371	40.5	39.0	41.0	37.0	41.0
28-29	39.31039329308605	40.0	39.0	41.0	36.0	41.0
30-31	39.21516845209848	40.0	39.0	41.0	36.0	41.0
32-33	39.107886412397406	40.0	39.0	41.0	35.5	41.0
34-35	39.034009766945275	40.0	39.0	41.0	35.0	41.0
36-37	39.03655893359122	40.0	39.0	41.0	35.0	41.0
38-39	39.02873019380985	40.0	39.0	41.0	35.0	41.0
40-41	38.97864266861346	40.0	39.0	41.0	35.0	41.0
42-43	38.938687757431055	40.0	38.0	41.0	35.0	41.0
44-45	38.903948316496525	40.0	38.0	41.0	35.0	41.0
46-47	38.82770032780396	40.0	38.0	41.0	35.0	41.0
48-49	38.79592516202017	40.0	38.0	41.0	35.0	41.0
50-51	38.701539136829105	40.0	38.0	41.0	35.0	41.0
52-53	38.529512076381764	40.0	38.0	41.0	34.5	41.0
54-55	38.392224140857905	40.0	38.0	41.0	34.0	41.0
56-57	38.2302381134024	40.0	37.0	41.0	34.0	41.0
58-59	38.03923012937962	40.0	37.0	41.0	34.0	41.0
60-61	37.90880288731876	39.0	36.5	41.0	34.0	41.0
62-63	37.73892936018788	39.0	36.0	41.0	33.5	41.0
64-65	37.484046593840525	39.0	35.5	40.5	33.0	41.0
66-67	37.21312375915882	38.5	35.0	40.0	33.0	41.0
68-69	36.9556071024205	38.0	35.0	40.0	33.0	41.0
70-71	36.65955293551569	37.0	35.0	40.0	32.5	41.0
72-73	36.30914609121089	37.0	35.0	39.0	32.0	41.0
74-75	35.93360188347749	36.0	35.0	39.0	32.0	40.5
76-77	34.0031883829074	34.5	32.0	36.5	29.0	39.0
78-79	35.0702840375968	35.0	34.0	37.0	31.0	39.0
80-81	35.003610831328885	35.0	34.0	37.0	31.0	39.0
82-83	34.72968908476503	35.0	34.0	36.5	31.0	39.0
84-85	34.48726164466382	35.0	34.0	36.0	31.0	37.0
86-87	34.28462362926898	35.0	34.0	36.0	31.0	37.0
88-89	34.131518791650265	35.0	34.0	35.5	31.0	37.0
90-91	33.992013560051795	35.0	34.0	35.0	31.0	36.0
92-93	33.907958834185564	35.0	34.0	35.0	31.0	36.0
94-95	33.82740995580262	35.0	34.0	35.0	31.0	36.0
96-97	33.79133371214957	35.0	34.0	35.0	31.0	36.0
98-99	33.82259924630836	35.0	34.0	35.0	31.0	35.0
100-101	32.54703694739284	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	15.0
20	27.0
21	131.0
22	503.0
23	1781.0
24	4672.0
25	10219.0
26	19263.0
27	32562.0
28	49652.0
29	70412.0
30	95347.0
31	127271.0
32	170239.0
33	238100.0
34	362134.0
35	592424.0
36	1158793.0
37	2463880.0
38	3279328.0
39	1391763.0
40	25384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.51738747982569	20.013134664189792	13.135268515116207	24.334209340868313
2	15.028844697328076	21.416168584478342	40.55714733368714	22.997839384506438
3	18.28817710197684	25.96625391999694	30.57659535070257	25.16897362732365
4	12.34870810067627	17.626183660663106	39.220695513269504	30.804412725391124
5	12.9458980176146	38.094106341453745	36.06289937486997	12.89709626606168
6	30.178689091561328	38.24507492197516	18.674821558087405	12.901414428376107
7	26.555758769577785	32.52997032564516	23.493265884022442	17.421005020754613
8	24.671326997526315	36.84395057701984	21.360686366654853	17.12403605879899
9	25.809620635807878	16.100771195593914	21.10022729078538	36.98938087781283
10-11	24.21543380470636	26.620947559893267	29.426538174062213	19.737080461338163
12-13	24.85618247199017	24.219701923950453	29.414232424500934	21.50988317955844
14-15	21.92418926800997	25.68414795587924	26.725639650729217	25.666023125381578
16-17	21.760837151702788	28.770422291021674	27.432054489164088	22.036686068111454
18-19	21.82098873369176	28.16450250502754	28.852621003514894	21.161887757765804
20-21	22.525503049111233	27.41894688286049	28.69975391329771	21.35579615473057
22-23	22.216126050749462	27.354978974978884	28.563403092415225	21.86549188185643
24-25	22.012380234632847	27.41303834027758	28.667933131075596	21.906648294013976
26-27	21.98894577434076	27.618201619745957	28.55218966364029	21.84066294227299
28-29	21.933335270744443	27.723182568695687	28.547136071622585	21.796346088937288
30-31	22.19628527789912	27.569641729895935	28.468690751266863	21.765382240938088
32-33	21.977142127677276	27.73914558017307	28.415058270505465	21.86865402164419
34-35	22.113764049847585	27.730061967817115	28.4287922754855	21.727381706849798
36-37	22.09595644747095	27.66943456600442	28.39631483530553	21.838294151219095
38-39	21.935515092136942	27.783255190607502	28.491688698153283	21.78954101910227
40-41	22.15765487063587	27.678700610372292	28.352870520426716	21.810773998565125
42-43	22.019727230906117	27.80280461812031	28.211559339475063	21.96590881149851
44-45	22.13105006418327	27.84666947012698	28.083206935114358	21.939073530575396
46-47	22.158404713337525	27.833323906519915	27.885214622596227	22.123056757546337
48-49	22.102885580830478	27.925721908393403	27.90612658994644	22.065265920829678
50-51	22.09839229875473	28.053735662315386	27.79258490753915	22.05528713139073
52-53	22.292729507951414	27.92573639651021	27.710854344667784	22.07067975087059
54-55	22.196340254303625	27.833423203514407	27.79953666928869	22.170699872893284
56-57	22.20593744910256	27.95207853291665	27.737940296190526	22.10404372179026
58-59	22.20032208690004	27.982839892302362	27.69215796856167	22.124680052235924
60-61	22.225427853319545	28.005300312009034	27.729024832669367	22.04024700200205
62-63	22.215708833668256	28.028129148258053	27.703028551128934	22.053133466944765
64-65	22.19308217519493	27.942620744795544	27.68476720951582	22.179529870493703
66-67	22.238489210654272	28.006700876720362	27.546231276165013	22.208578636460356
68-69	22.202655762666414	28.046237401941188	27.609513107119373	22.14159372827303
70-71	22.206998212861404	27.83116678594255	27.647416039176203	22.31441896201984
72-73	22.214445131171345	27.888267138118483	27.635199944520377	22.26208778618979
74-75	22.097451888710594	27.983661975630813	27.65169860478237	22.26718753087622
76-77	22.249588597712407	27.927585784729835	27.53821820844287	22.284607409114887
78-79	22.212465999493297	27.9504663440225	27.52400420516563	22.313063451318573
80-81	22.182716320432625	27.970033811499334	27.548144927242134	22.299104940825902
82-83	22.281168830968063	27.930182535925773	27.595706993958963	22.192941639147197
84-85	22.290165339993926	27.92909189458821	27.558672685759976	22.22207007965789
86-87	22.1599063409186	27.944837957421694	27.637711943442376	22.25754375821733
88-89	22.211002236723306	27.941629027988125	27.57608573997223	22.271282995316337
90-91	22.24443780109866	27.956014710183318	27.594258592883158	22.205288895834858
92-93	22.271691253619945	27.918646730373737	27.617167830509988	22.19249418549633
94-95	22.15184283025937	27.93545130634992	27.67458293616781	22.238122927222907
96-97	22.16461882964073	28.003738422024604	27.67368125242567	22.157961495909
98-99	22.421380804734355	28.559448866264113	27.7179357816475	21.301234547354028
100-101	23.14662481341527	31.135942108521526	24.841348745226373	20.876084332836836
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2256.0
1	1879.5
2	2285.0
3	3301.5
4	4431.0
5	7051.0
6	9352.0
7	11103.5
8	12389.5
9	12372.0
10	11119.0
11	9648.0
12	10235.5
13	13287.5
14	20564.5
15	30307.5
16	37682.0
17	41807.5
18	43419.0
19	42824.5
20	40995.5
21	39662.0
22	40824.5
23	46476.5
24	56723.5
25	71589.0
26	90626.5
27	111163.5
28	133596.5
29	157295.0
30	178535.0
31	199616.0
32	221406.0
33	242695.5
34	263539.5
35	283469.5
36	305586.0
37	323973.5
38	338737.0
39	354478.5
40	369267.0
41	382626.5
42	391773.5
43	399909.5
44	408496.5
45	416012.0
46	421088.0
47	422706.0
48	421436.0
49	416588.0
50	409202.0
51	397716.5
52	381446.0
53	361989.5
54	340285.5
55	316603.5
56	289921.5
57	261744.5
58	233621.5
59	203553.5
60	170993.0
61	140171.5
62	112530.0
63	87468.5
64	65995.5
65	49738.5
66	37865.0
67	28985.0
68	21982.5
69	15951.5
70	11651.0
71	8550.0
72	6221.0
73	4508.5
74	3144.0
75	2159.5
76	1450.0
77	947.5
78	641.5
79	447.0
80	320.0
81	222.5
82	160.0
83	112.5
84	80.5
85	55.0
86	43.0
87	39.5
88	29.5
89	18.0
90	11.5
91	8.5
92	6.5
93	5.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.783255056692155E-4
3	0.004577021312176531
4	2.972091761153592E-5
5	9.90697253717864E-6
6	0.0
7	0.0
8	5.349765170076465E-4
9	0.0022092548757908365
10-11	8.965810146146669E-4
12-13	3.368370662640737E-4
14-15	1.3374412925191162E-4
16-17	0.0014959528531139745
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	6.156641849772974E-4
32-33	6.666349932130091E-4
34-35	2.9926978173257255E-5
36-37	7.761153942388303E-4
38-39	6.344907031249724E-4
40-41	1.5203179856556985E-5
42-43	7.651902117480265E-5
44-45	9.100280463445922E-4
46-47	0.0
48-49	5.131831515059098E-4
50-51	2.1129979537199566E-5
52-53	0.0
54-55	5.905413105657826E-4
56-57	7.962076682923025E-4
58-59	1.1481611160913359E-4
60-61	1.600666495450547E-4
62-63	1.6712103161361708E-5
64-65	5.621030252778292E-6
66-67	0.0
68-69	1.1469201237802074E-5
70-71	4.289160601416826E-4
72-73	0.0
74-75	5.916401133653454E-6
76-77	0.0
78-79	2.6585837663854105E-4
80-81	0.0
82-83	6.183570673204103E-6
84-85	1.875729893394767E-5
86-87	2.655589066560443E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	201.0
20-21	604.0
22-23	1182.0
24-25	3110.0
26-27	4699.0
28-29	9327.0
30-31	18897.0
32-33	23991.0
34-35	34080.0
36-37	52634.0
38-39	63138.0
40-41	63716.0
42-43	72175.0
44-45	87725.0
46-47	88707.0
48-49	84952.0
50-51	77057.0
52-53	74501.0
54-55	80682.0
56-57	85933.0
58-59	86230.0
60-61	84781.0
62-63	79982.0
64-65	83926.0
66-67	89914.0
68-69	92151.0
70-71	91577.0
72-73	85669.0
74-75	87283.0
76-77	86759.0
78-79	93892.0
80-81	95795.0
82-83	89753.0
84-85	88381.0
86-87	92513.0
88-89	99926.0
90-91	108705.0
92-93	106478.0
94-95	118772.0
96-97	186306.0
98-99	859767.0
100-101	6358030.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.10890633423553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.72812204339519	76.36662334538174
2	7.148669051329927	12.168308094081741
3	1.762305996358139	4.499638079289193
4	0.633017443756665	2.1550168931449285
5	0.29226397015884364	1.2437133430560414
6	0.14281006535665935	0.7292645085615584
7	0.08156006270960346	0.48590414164362117
8	0.058042577876118974	0.39519522590849443
9	0.036099104820636434	0.2765119797836367
>10	0.11470417691508429	1.4911886464070763
>50	0.001990795958934803	0.10923732082644368
>100	3.5821455437599887E-4	0.0497794188100967
>500	5.649680982495635E-5	0.029619003105494365
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.9720917611535916E-5	0.0	0.0	0.0
3	0.0	2.9720917611535916E-5	0.0	1.981394507435728E-5	0.0
4	0.0	8.916275283460775E-5	0.0	1.981394507435728E-5	1.981394507435728E-5
5	0.0	1.0897669790896503E-4	0.0	1.981394507435728E-5	1.981394507435728E-5
6	0.0	1.0897669790896503E-4	0.0	1.981394507435728E-5	1.981394507435728E-5
7	0.0	1.287906429833223E-4	0.0	1.981394507435728E-5	1.981394507435728E-5
8	0.0	1.287906429833223E-4	0.0	1.981394507435728E-5	3.962789014871456E-5
9	0.0	2.1795339581793006E-4	0.0	4.953486268589319E-5	7.925578029742911E-5
10-11	0.0	2.278603683551087E-4	0.0	5.448834895448251E-5	7.925578029742911E-5
12-13	0.0	2.278603683551087E-4	0.0	5.944183522307183E-5	8.420926656601844E-5
14-15	0.0	3.56651011338431E-4	0.0	6.439532149166114E-5	1.4365110178909024E-4
16-17	0.0	3.7151147014419895E-4	0.0	6.934880776025047E-5	1.6841853313203685E-4
18-19	0.0	4.4581376417303874E-4	0.0	6.934880776025047E-5	1.832789919378048E-4
20-21	0.0	4.953486268589319E-4	0.0	6.934880776025047E-5	1.9813945074357276E-4
22-23	0.0	5.300230307390572E-4	0.0	6.934880776025047E-5	2.0309293701216207E-4
24-25	0.0	5.646974346191824E-4	0.0	1.0897669790896502E-4	2.1795339581793006E-4
26-27	0.0	5.845113796935396E-4	0.0	1.783255056692155E-4	2.1795339581793006E-4
28-29	0.0	5.993718384993075E-4	0.0	5.102090856646999E-4	2.2290688208651937E-4
30-31	0.0	6.588136737223795E-4	0.0	0.001317627347444759	2.3281385462369802E-4
32-33	0.0	6.736741325281474E-4	0.0	0.0029275103847362875	2.526277996980553E-4
34-35	0.0	8.024647755114698E-4	0.0	0.004993114158738034	2.6748825850382324E-4
36-37	0.0	9.312554184947921E-4	0.0	0.008698321887642845	2.6748825850382324E-4
38-39	0.0	9.857437674492746E-4	0.0	0.014142203296822507	2.7739523104100186E-4
40-41	0.0	0.0010006042262550425	0.0	0.019927875258534834	2.7739523104100186E-4
42-43	0.0	0.0010451856026723464	0.0	0.025356896208908727	2.823487173095912E-4
44-45	0.0	0.001134348355506954	0.0	0.03139519597031911	2.922556898467698E-4
46-47	0.0	0.0011987436769986153	0.0	0.03830530931500121	2.9720917611535916E-4
48-49	0.0	0.0012036971632672045	0.0	0.04555225972594738	3.1702312118971646E-4
50-51	4.95348626858932E-6	0.0013275343199819377	0.0	0.05244751261182372	3.269300937268951E-4
52-53	9.90697253717864E-6	0.0013374412925191163	0.0	0.058901905219795596	3.6655798387560967E-4
54-55	9.90697253717864E-6	0.0013523017513248842	0.0	0.0658516464546264	4.111393602929135E-4
56-57	9.90697253717864E-6	0.0014067901002793666	0.0	0.07195929502379704	4.160928465615028E-4
58-59	9.90697253717864E-6	0.0014414645041594919	0.0	0.07767066469148054	4.160928465615028E-4
60-61	9.90697253717864E-6	0.0014810923943082066	0.0	0.0837139179391595	4.160928465615028E-4
62-63	9.90697253717864E-6	0.001500906339382564	0.0	0.08917265980714492	4.359067916358601E-4
64-65	9.90697253717864E-6	0.001694092303857547	0.0	0.09459672727125024	4.408602779044494E-4
66-67	9.90697253717864E-6	0.001718859735200494	0.0	0.1001743528096818	4.507672504416281E-4
68-69	9.90697253717864E-6	0.0017485806528120297	0.0	0.10667828028033957	4.557207367102174E-4
70-71	9.90697253717864E-6	0.001768394597886387	0.0	0.11246395224205191	4.557207367102174E-4
72-73	9.90697253717864E-6	0.0017733480841549763	0.0	0.11701620612288549	5.448834895448252E-4
74-75	9.90697253717864E-6	0.0018030690017665123	0.0	0.12054804183238968	5.448834895448252E-4
76-77	9.90697253717864E-6	0.0018526038644524055	0.0	0.12392631946756759	5.448834895448252E-4
78-79	9.90697253717864E-6	0.0018724178095267628	0.0	0.126611109025143	5.448834895448252E-4
80-81	9.90697253717864E-6	0.0018724178095267628	0.0	0.12801789912542239	5.646974346191824E-4
82-83	9.90697253717864E-6	0.0018971852408697094	0.0	0.12868166628541333	6.04325324767897E-4
84-85	9.90697253717864E-6	0.0019566270760927812	0.0	0.12917701491227226	6.092788110364862E-4
86-87	9.90697253717864E-6	0.002011115425047264	0.0	0.12942468922570174	6.191857835736649E-4
88-89	9.90697253717864E-6	0.00211018515041905	0.0	0.12948413106092482	6.389997286480222E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10380	0.0	45.989216	1
GTATCAA	17415	0.0	33.816288	1
TCAACGC	20055	0.0	28.320179	4
ATCAACG	20350	0.0	27.910608	3
CAACGCA	20570	0.0	27.548954	5
AACGCAG	21325	0.0	26.674196	6
TATCAAC	22410	0.0	25.878443	2
ACGCAGA	25195	0.0	22.543062	7
CGCAGAG	25645	0.0	22.080835	8
GTACATG	26065	0.0	21.642946	1
TACATGG	25845	0.0	21.298033	2
ACATGGG	26250	0.0	20.383736	3
CATGGGG	19535	0.0	18.92328	4
GCAGAGT	31695	0.0	17.946997	9
GAGTACT	18420	0.0	17.92262	12-13
AGAGTAC	28060	0.0	17.408266	10-11
CAGAGTA	30900	0.0	16.880148	10-11
GTGGTAT	5750	0.0	16.871674	1
GTACTTT	20970	0.0	15.753768	14-15
GAGTACA	19320	0.0	15.484282	1
>>END_MODULE