FastQCFastQC Report
Fri 27 May 2016
SRR2140014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8184774
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82250.043.046341
GTATCAA141200.033.363991
ATCAACG162000.028.0737193
TCAACGC161850.028.0725384
CAACGCA164450.027.5526185
AACGCAG171850.026.4636346
TATCAAC175400.026.334282
GTACATG244050.022.2836951
ACGCAGA204350.022.2139267
CGCAGAG206050.022.0103668
TACATGG239550.021.9694042
ACATGGG243150.021.1454493
CATGGGG184750.018.6200244
GCAGAGT254900.017.677439
AGAGTAC220300.016.92421510-11
GAGTACT126350.016.4906412-13
CAGAGTA245550.016.24778710-11
GTGGTAT47800.015.1288841
AGTACTT143000.014.62903512-13
ATGGGGG111200.014.5845985
Produced by FastQC (version 0.11.5)