##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140014_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8184774
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68610544408435	34.0	31.0	34.0	31.0	34.0
2	32.86850021759917	34.0	31.0	34.0	31.0	34.0
3	32.96740887408742	34.0	31.0	34.0	31.0	34.0
4	36.37050577083741	37.0	37.0	37.0	35.0	37.0
5	36.20796701289492	37.0	37.0	37.0	35.0	37.0
6	36.26162041859678	37.0	37.0	37.0	35.0	37.0
7	36.26099034622092	37.0	37.0	37.0	35.0	37.0
8	36.242755267280444	37.0	37.0	37.0	35.0	37.0
9	38.133832406368214	39.0	39.0	39.0	37.0	39.0
10-11	38.02620225066691	39.0	38.0	39.0	36.0	39.0
12-13	38.064491762387085	39.0	38.0	39.0	36.0	39.0
14-15	39.48230323769477	40.5	39.0	41.0	37.0	41.0
16-17	39.461231428015	40.0	39.0	41.0	37.0	41.0
18-19	39.50686787442146	40.5	39.0	41.0	37.0	41.0
20-21	39.521100009689654	41.0	39.0	41.0	37.0	41.0
22-23	39.455575126769276	40.0	39.0	41.0	37.0	41.0
24-25	39.41204965520711	41.0	39.0	41.0	37.0	41.0
26-27	39.37383202641881	40.0	39.0	41.0	36.5	41.0
28-29	39.27750580620054	40.0	39.0	41.0	36.0	41.0
30-31	39.185786169718796	40.0	39.0	41.0	36.0	41.0
32-33	39.080741077862314	40.0	39.0	41.0	35.5	41.0
34-35	39.00658706118437	40.0	39.0	41.0	35.0	41.0
36-37	39.00990178725574	40.0	39.0	41.0	35.0	41.0
38-39	39.00310649000697	40.0	38.5	41.0	35.0	41.0
40-41	38.959672604761245	40.0	38.0	41.0	35.0	41.0
42-43	38.9204878702798	40.0	38.0	41.0	35.0	41.0
44-45	38.88618319205732	40.0	38.0	41.0	35.0	41.0
46-47	38.81010331508804	40.0	38.0	41.0	35.0	41.0
48-49	38.77912744240201	40.0	38.0	41.0	35.0	41.0
50-51	38.678085683922916	40.0	38.0	41.0	35.0	41.0
52-53	38.5013845036775	40.0	38.0	41.0	34.5	41.0
54-55	38.358841324456606	40.0	37.5	41.0	34.0	41.0
56-57	38.18930150683724	40.0	37.0	41.0	34.0	41.0
58-59	37.993363682732316	40.0	37.0	41.0	34.0	41.0
60-61	37.851403207508376	39.0	36.0	41.0	34.0	41.0
62-63	37.6748143350172	39.0	36.0	41.0	33.5	41.0
64-65	37.414048068298044	39.0	35.5	40.5	33.0	41.0
66-67	37.13828083019599	38.5	35.0	40.0	33.0	41.0
68-69	36.87745587799067	37.5	35.0	40.0	33.0	41.0
70-71	36.577339770932866	37.0	35.0	39.5	32.5	41.0
72-73	36.226407819363345	36.5	35.0	39.0	32.0	41.0
74-75	35.847939668475505	36.0	35.0	39.0	32.0	40.5
76-77	33.93280668445251	34.5	32.0	36.5	29.0	39.0
78-79	35.00978183269523	35.0	34.0	37.0	31.0	39.0
80-81	34.947220153320444	35.0	34.0	37.0	31.0	39.0
82-83	34.681874958975115	35.0	34.0	36.5	31.0	39.0
84-85	34.44414975745913	35.0	34.0	36.0	31.0	37.0
86-87	34.24795768213261	35.0	34.0	36.0	31.0	37.0
88-89	34.10126176324381	35.0	34.0	35.0	31.0	36.5
90-91	33.96832244348953	35.0	34.0	35.0	31.0	36.0
92-93	33.88976413351074	35.0	34.0	35.0	31.0	36.0
94-95	33.81241138852839	35.0	34.0	35.0	31.0	36.0
96-97	33.77920249886454	35.0	34.0	35.0	31.0	36.0
98-99	33.810427139746224	35.0	34.0	35.0	31.0	35.0
100-101	32.57449246333761	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	27.0
21	108.0
22	449.0
23	1416.0
24	3769.0
25	8050.0
26	15634.0
27	25925.0
28	39185.0
29	55690.0
30	76248.0
31	101542.0
32	137412.0
33	191983.0
34	295338.0
35	481210.0
36	942138.0
37	1986902.0
38	2619628.0
39	1179521.0
40	22593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.56629443891792	19.8662540957148	13.0964275861496	24.47102387921768
2	15.097208738486831	21.26138610329102	40.56374055286668	23.077664605355466
3	18.647159459820728	25.907489302201235	30.063081029578974	25.38227020839906
4	12.591954279983785	17.558383408020795	38.62217820553139	31.227484106464026
5	13.23583924777361	37.8889327372669	35.67943876248202	13.195789252477466
6	30.47170270064879	37.67061863895081	18.668249117202258	13.18942954319814
7	26.85027100320669	32.21404769392533	23.215301974129034	17.72037932873895
8	24.797308549297465	36.55317444153021	21.31064539567641	17.33887161349591
9	25.847878478723697	16.269323697320875	20.943262173051135	36.93953565090429
10-11	24.50770812989232	26.497829334664267	28.9711690803667	20.02329345507671
12-13	25.13275305061391	23.98906453541747	29.0944459363797	21.78373647758892
14-15	22.206225017986164	25.429665744159863	26.3737797677355	25.99032947011847
16-17	22.091734826729432	28.54944056292653	27.063892483691433	22.294932126652604
18-19	22.11766018218707	27.90736188928369	28.466186115829224	21.50879181270002
20-21	22.822916257482984	27.120834031554697	28.30098137494637	21.75526833601595
22-23	22.574906482025064	27.13352858362768	28.06882136039931	22.222743573947948
24-25	22.354704967998064	27.238257936595478	28.202786046534083	22.20425104887238
26-27	22.325468776925153	27.380647248332373	28.115435123904525	22.178448850837952
28-29	22.218452806810298	27.447646041534746	28.15952114368821	22.174380007966747
30-31	22.476159001017937	27.303723108530754	28.016781063513868	22.203336826937438
32-33	22.26984157025295	27.460685054974928	28.050664238903316	22.218809135868803
34-35	22.401356305319915	27.51551196196563	28.042350067479777	22.04078166523468
36-37	22.38178219172349	27.425830882911427	27.99308043222497	22.19930649314011
38-39	22.213422134221343	27.518950189501894	28.0790182901829	22.18860938609386
40-41	22.420140711457268	27.368089629964633	28.00522099891363	22.206548659664474
42-43	22.301199873882315	27.46579032349593	27.90600618933481	22.32700361328694
44-45	22.37902347383326	27.566340784366698	27.73674492048899	22.31789082131105
46-47	22.505743017050293	27.535637907162684	27.480651850311038	22.477967225475982
48-49	22.411989612600056	27.610784928776305	27.522191699836807	22.45503375878684
50-51	22.34489612814122	27.729477050666418	27.515244540793315	22.41038228039905
52-53	22.54344322625688	27.590636785044808	27.40408367602552	22.461836312672787
54-55	22.48209221330043	27.523693024563052	27.44632818481075	22.547886577325773
56-57	22.504550544937484	27.646606329164	27.37941887276761	22.469424253130907
58-59	22.445843599880995	27.755892761416412	27.38879361101152	22.40947002769107
60-61	22.55215795403757	27.725718251115122	27.33836609591105	22.383757698936265
62-63	22.489871830215936	27.800419613341077	27.31633332231353	22.393375234129458
64-65	22.46865785902558	27.765549790722478	27.279053693657357	22.48673865659459
66-67	22.547367513747503	27.713282232197894	27.215403126665112	22.523947127389487
68-69	22.49875715690371	27.67239170008452	27.288501883907806	22.540349259103966
70-71	22.50076263340138	27.466256816872246	27.35053871947757	22.682441830248806
72-73	22.54256372147877	27.557230492442475	27.297508303648794	22.60269748242996
74-75	22.399285664463562	27.653780630258566	27.305489571843655	22.641444133434216
76-77	22.551564268285844	27.61441344372919	27.19963266010511	22.634389627879855
78-79	22.526245633513746	27.592159964931856	27.164708552493018	22.716885849061377
80-81	22.46731071485059	27.644020272331993	27.215900588051532	22.672768424765877
82-83	22.580251333769404	27.610831255174105	27.282770612103064	22.526146798953427
84-85	22.63318216943815	27.610510817805995	27.20129829511888	22.555008717636976
86-87	22.501535876521253	27.610208854665586	27.29113101865221	22.59712425016095
88-89	22.533475085043154	27.5767957345873	27.26530439063739	22.624424789732156
90-91	22.564442181363866	27.611505982809142	27.24032789415104	22.583723941675952
92-93	22.60360340376448	27.551347394536705	27.2836942481704	22.56135495352841
94-95	22.457411981498794	27.61340165238454	27.35679372983366	22.572392636283002
96-97	22.50786585495435	27.62061646293174	27.338917404753293	22.532600277360615
98-99	22.77824093984063	28.189246918056032	27.386589305555354	21.64592283654799
100-101	26.96201979793895	35.23591706442829	27.935297890308192	9.86676524732456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1440.0
1	1265.5
2	1714.5
3	2499.5
4	3341.5
5	5292.5
6	6994.5
7	8076.0
8	8714.5
9	8518.5
10	7677.0
11	6863.5
12	7406.5
13	9746.0
14	15065.0
15	22315.0
16	28276.5
17	31781.0
18	32805.5
19	31984.0
20	30702.5
21	29900.0
22	30779.0
23	35084.0
24	42888.0
25	54106.0
26	68905.5
27	85072.5
28	102509.0
29	121285.5
30	138562.5
31	155270.0
32	172299.0
33	188206.5
34	204007.0
35	221338.0
36	240821.0
37	258261.5
38	273134.0
39	286584.5
40	299013.5
41	311886.5
42	322049.0
43	331343.0
44	340722.0
45	349358.0
46	355594.0
47	359439.5
48	363345.0
49	361359.0
50	355881.0
51	348779.5
52	335929.5
53	320995.0
54	304627.0
55	284303.0
56	261970.0
57	238220.0
58	212352.0
59	184527.5
60	155230.0
61	126933.0
62	101857.0
63	80021.0
64	60635.0
65	45910.0
66	35003.5
67	26819.0
68	20456.0
69	14835.0
70	10747.5
71	7873.5
72	5684.5
73	4029.0
74	2836.0
75	1999.0
76	1403.0
77	943.0
78	578.0
79	378.5
80	276.5
81	188.5
82	135.0
83	95.0
84	62.0
85	45.0
86	37.0
87	26.5
88	16.0
89	10.0
90	7.0
91	5.0
92	2.5
93	1.0
94	1.0
95	1.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.954849333652951E-4
3	0.004642767167425759
4	0.0
5	4.8871233341323775E-5
6	0.0
7	0.0
8	5.253657584192307E-4
9	0.002431343858730858
10-11	8.735732959761625E-4
12-13	3.420986333892665E-4
14-15	1.2828698752097493E-4
16-17	0.0014417013835690516
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	6.372018798925923E-4
32-33	8.356836937219262E-4
34-35	2.4668163859758527E-5
36-37	8.556381563279434E-4
38-39	6.750021937571298E-4
40-41	2.5223429135280312E-5
42-43	1.081516507536357E-4
44-45	9.832023414353276E-4
46-47	0.0
48-49	5.785646783371052E-4
50-51	3.32349423942067E-5
52-53	2.6857624375979884E-5
54-55	6.578714900267021E-4
56-57	7.885012114121221E-4
58-59	8.321878037052052E-5
60-61	1.4030411899921912E-4
62-63	2.1282879477110866E-5
64-65	0.0
66-67	0.0
68-69	2.2029710295357466E-5
70-71	4.608484696039959E-4
72-73	0.0
74-75	2.2819300198999512E-5
76-77	0.0
78-79	3.114390391171326E-4
80-81	0.0
82-83	0.0
84-85	8.081613303369944E-6
86-87	2.3723202903981806E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	205.0
20-21	505.0
22-23	1138.0
24-25	2617.0
26-27	4383.0
28-29	9960.0
30-31	22615.0
32-33	27507.0
34-35	37896.0
36-37	59946.0
38-39	71711.0
40-41	69193.0
42-43	75091.0
44-45	87756.0
46-47	87958.0
48-49	84491.0
50-51	76911.0
52-53	73255.0
54-55	78813.0
56-57	82318.0
58-59	82488.0
60-61	80824.0
62-63	76338.0
64-65	78643.0
66-67	82832.0
68-69	82179.0
70-71	80536.0
72-73	74841.0
74-75	74843.0
76-77	75400.0
78-79	79966.0
80-81	80346.0
82-83	75933.0
84-85	74202.0
86-87	77135.0
88-89	82748.0
90-91	89115.0
92-93	87537.0
94-95	95234.0
96-97	148068.0
98-99	670961.0
100-101	4880336.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.38979642366034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.22651295571858	77.9465008626128
2	7.037364535346398	12.159129791353248
3	1.612179568915052	4.178275942708674
4	0.5547922505870794	1.9171355834256856
5	0.2321123424227666	1.0026069004660874
6	0.12111545186468103	0.6277883538209656
7	0.06550816147911052	0.3961465713988039
8	0.04265919066379004	0.2948255037634347
9	0.024778818658333465	0.19265733895610018
>10	0.08082579525438396	1.0944235312687711
>50	0.001665227725865653	0.10037239242738699
>100	4.4264995488078554E-4	0.06427021259591469
>500	4.3051409113087617E-5	0.02586701520216878
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.2217808335330946E-5	0.0	0.0	0.0
2	0.0	2.443561667066189E-5	0.0	0.0	0.0
3	0.0	2.443561667066189E-5	0.0	2.443561667066189E-5	0.0
4	0.0	3.665342500599284E-5	0.0	2.443561667066189E-5	0.0
5	0.0	3.665342500599284E-5	0.0	2.443561667066189E-5	0.0
6	7.330685001198568E-5	3.665342500599284E-5	0.0	2.443561667066189E-5	1.2217808335330946E-5
7	7.330685001198568E-5	4.887123334132378E-5	0.0	3.665342500599284E-5	1.2217808335330946E-5
8	7.330685001198568E-5	4.887123334132378E-5	0.0	3.665342500599284E-5	1.2217808335330946E-5
9	7.330685001198568E-5	1.0996027501797851E-4	0.0	7.330685001198568E-5	2.443561667066189E-5
10-11	8.552465834731662E-5	1.2217808335330944E-4	0.0	7.330685001198568E-5	2.443561667066189E-5
12-13	9.774246668264756E-5	1.282869875209749E-4	0.0	8.552465834731662E-5	2.443561667066189E-5
14-15	9.774246668264756E-5	1.954849333652951E-4	0.0	1.0996027501797851E-4	3.665342500599284E-5
16-17	1.771582208622987E-4	2.1381164586829152E-4	0.0	1.282869875209749E-4	3.665342500599284E-5
18-19	1.9548493336529513E-4	2.1992055003595702E-4	0.0	1.6494041252696775E-4	3.665342500599284E-5
20-21	1.9548493336529513E-4	2.1992055003595702E-4	0.0	2.1992055003595702E-4	3.665342500599284E-5
22-23	2.1381164586829152E-4	2.5046507087428437E-4	0.0	2.810095917126118E-4	4.8871233341323775E-5
24-25	2.443561667066189E-4	3.0544520838327364E-4	0.0	3.2377192088627006E-4	6.108904167665472E-5
26-27	2.443561667066189E-4	3.35989729221601E-4	0.0	3.6653425005992834E-4	6.108904167665472E-5
28-29	2.443561667066189E-4	3.482075375569319E-4	0.0	6.658705542755365E-4	6.108904167665472E-5
30-31	2.6268287920961535E-4	3.9707877089825575E-4	0.0	0.0017899089211259834	6.108904167665472E-5
32-33	2.687917833772808E-4	4.092965792335866E-4	0.0	0.004184599354850849	8.552465834731662E-5
34-35	2.810095917126118E-4	4.948212375809033E-4	0.0	0.007006913080312297	8.552465834731662E-5
36-37	2.932274000479427E-4	5.8034589592822E-4	0.0	0.011613026822732063	9.774246668264756E-5
38-39	2.932274000479427E-4	6.047815125988817E-4	0.0	0.0185771775738707	9.774246668264756E-5
40-41	3.0544520838327364E-4	6.475438417725401E-4	0.0	0.025822337916721953	9.774246668264756E-5
42-43	3.0544520838327364E-4	6.658705542755365E-4	0.0	0.032847577709537244	9.774246668264756E-5
44-45	3.298808250539355E-4	7.147417876168603E-4	0.0	0.040666975044149054	9.774246668264756E-5
46-47	3.543164417245974E-4	7.575041167905185E-4	0.0	0.0491339162205334	1.1606917918564397E-4
48-49	3.604253458922629E-4	7.575041167905185E-4	0.0	0.05738093684688178	1.2217808335330944E-4
50-51	3.970787708982557E-4	8.491376793055008E-4	0.0	0.0654630170607032	1.2217808335330944E-4
52-53	4.154054834012521E-4	8.552465834731662E-4	0.0	0.07302584042027305	1.282869875209749E-4
54-55	4.154054834012521E-4	8.552465834731662E-4	0.0	0.08090632679656151	1.343958916886404E-4
56-57	4.154054834012521E-4	8.674643918084971E-4	0.0	0.08879292207701764	1.405047958563059E-4
58-59	4.154054834012521E-4	8.735732959761625E-4	0.0	0.09607473584487489	1.4661370002397135E-4
60-61	4.2762329173658305E-4	8.919000084791589E-4	0.0	0.10347261879191777	1.4661370002397135E-4
62-63	4.642767167425759E-4	9.041178168144899E-4	0.0	0.11028404693886477	1.4661370002397135E-4
64-65	4.642767167425759E-4	9.407712418204828E-4	0.0	0.11716878193582377	1.5272260419163682E-4
66-67	4.703856209102414E-4	9.407712418204828E-4	0.0	0.12418180392030373	1.5883150835930228E-4
68-69	4.764945250779069E-4	9.529890501558138E-4	0.0	0.13109097453393337	1.5883150835930228E-4
70-71	4.887123334132379E-4	9.713157626588101E-4	0.0	0.1377069177475151	1.5883150835930228E-4
72-73	5.131479500838997E-4	9.83533570994141E-4	0.0	0.14291170409836607	1.8326712502996417E-4
74-75	5.131479500838997E-4	0.0010140780918324686	0.0	0.14698634317819895	1.8326712502996417E-4
76-77	5.192568542515652E-4	0.0010751671335091232	0.0	0.15074942814548087	1.8326712502996417E-4
78-79	5.375835667545616E-4	0.001087384941844454	0.0	0.15404823639602022	1.8937602919762966E-4
80-81	5.375835667545616E-4	0.001087384941844454	0.0	0.15544106654624795	2.015938375329606E-4
82-83	5.375835667545616E-4	0.0010934938460121196	0.0	0.15605806586718218	2.1992055003595702E-4
84-85	5.436924709222271E-4	0.0010996027501797851	0.0	0.15657121381726608	2.1992055003595702E-4
86-87	5.620191834252236E-4	0.0011179294626827816	0.0	0.156827787792308	2.1992055003595702E-4
88-89	5.742369917605544E-4	0.0011606917918564397	0.0	0.156937748067326	2.1992055003595702E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8225	0.0	43.04634	1
GTATCAA	14120	0.0	33.36399	1
ATCAACG	16200	0.0	28.073719	3
TCAACGC	16185	0.0	28.072538	4
CAACGCA	16445	0.0	27.552618	5
AACGCAG	17185	0.0	26.463634	6
TATCAAC	17540	0.0	26.33428	2
GTACATG	24405	0.0	22.283695	1
ACGCAGA	20435	0.0	22.213926	7
CGCAGAG	20605	0.0	22.010366	8
TACATGG	23955	0.0	21.969404	2
ACATGGG	24315	0.0	21.145449	3
CATGGGG	18475	0.0	18.620024	4
GCAGAGT	25490	0.0	17.67743	9
AGAGTAC	22030	0.0	16.924215	10-11
GAGTACT	12635	0.0	16.49064	12-13
CAGAGTA	24555	0.0	16.247787	10-11
GTGGTAT	4780	0.0	15.128884	1
AGTACTT	14300	0.0	14.629035	12-13
ATGGGGG	11120	0.0	14.584598	5
>>END_MODULE