FastQCFastQC Report
Fri 27 May 2016
SRR2140013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9506630
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90750.042.154731
GTATCAA156200.035.275981
TCAACGC178400.030.0962874
ATCAACG179150.029.924443
CAACGCA181700.029.5496835
AACGCAG189400.028.3923136
TATCAAC196800.027.6190192
ACGCAGA223300.024.1191357
CGCAGAG227900.023.5776888
TACATGG244400.021.9682332
GTACATG250750.021.9248681
ACATGGG249200.020.7467633
GCAGAGT280950.019.066599
CATGGGG178150.018.7958154
AGAGTAC247900.017.38987510-11
GAGTACT155550.017.1113912-13
CAGAGTA273600.016.61321810-11
GTACTTT180900.014.73700314-15
AGTACTT176350.014.5637712-13
TATACTG55250.014.4192425
Produced by FastQC (version 0.11.5)