##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140013_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9506630
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.650149527224684	34.0	31.0	34.0	31.0	34.0
2	32.854702244644	34.0	31.0	34.0	31.0	34.0
3	32.956146710243274	34.0	31.0	34.0	31.0	34.0
4	36.37320059789852	37.0	37.0	37.0	35.0	37.0
5	36.19273265079213	37.0	35.0	37.0	35.0	37.0
6	36.23799653504975	37.0	37.0	37.0	35.0	37.0
7	36.24799376855942	37.0	37.0	37.0	35.0	37.0
8	36.236078189642384	37.0	37.0	37.0	35.0	37.0
9	38.15386209413851	39.0	39.0	39.0	37.0	39.0
10-11	38.045035569912784	39.0	38.0	39.0	36.0	39.0
12-13	38.09020604567549	39.0	38.0	39.0	37.0	39.0
14-15	39.51140835395929	40.5	39.0	41.0	37.0	41.0
16-17	39.49608015669065	40.0	39.0	41.0	37.0	41.0
18-19	39.54679523658751	41.0	39.5	41.0	37.0	41.0
20-21	39.567343024751025	41.0	39.0	41.0	37.0	41.0
22-23	39.50824924221717	41.0	39.0	41.0	37.0	41.0
24-25	39.470532904294686	41.0	39.0	41.0	37.0	41.0
26-27	39.4361357532694	40.5	39.0	41.0	37.0	41.0
28-29	39.344370081613214	40.0	39.0	41.0	36.5	41.0
30-31	39.255862289088654	40.0	39.0	41.0	36.0	41.0
32-33	39.155711767280266	40.0	39.0	41.0	36.0	41.0
34-35	39.08551352576039	40.0	39.0	41.0	35.0	41.0
36-37	39.09149622717479	40.0	39.0	41.0	35.0	41.0
38-39	39.08978616910575	40.0	39.0	41.0	35.0	41.0
40-41	39.049548680100614	40.0	39.0	41.0	35.0	41.0
42-43	39.01803839343336	40.0	39.0	41.0	35.0	41.0
44-45	38.986356696830796	40.0	39.0	41.0	35.0	41.0
46-47	38.9215286994277	40.0	38.0	41.0	35.0	41.0
48-49	38.89799893556909	40.0	38.0	41.0	35.0	41.0
50-51	38.80871670555587	40.0	38.0	41.0	35.0	41.0
52-53	38.64399147060853	40.0	38.0	41.0	35.0	41.0
54-55	38.51400258654336	40.0	38.0	41.0	34.5	41.0
56-57	38.36244762860888	40.0	37.5	41.0	34.0	41.0
58-59	38.17902091674037	40.0	37.0	41.0	34.0	41.0
60-61	38.05677880777482	40.0	37.0	41.0	34.0	41.0
62-63	37.89598731311543	39.0	36.0	41.0	34.0	41.0
64-65	37.65023325458971	39.0	36.0	41.0	33.5	41.0
66-67	37.38699356817254	39.0	35.0	40.0	33.0	41.0
68-69	37.13348105715565	38.0	35.0	40.0	33.0	41.0
70-71	36.837357486860554	37.5	35.0	40.0	33.0	41.0
72-73	36.486936773131305	37.0	35.0	39.0	33.0	41.0
74-75	36.104161502957695	36.5	35.0	39.0	32.0	40.5
76-77	34.15856922635761	34.5	33.0	36.5	29.0	39.0
78-79	35.19212348911997	35.0	34.0	37.0	31.0	39.0
80-81	35.11461189113898	35.0	34.0	37.0	32.0	39.0
82-83	34.83333347662112	35.0	34.0	37.0	32.0	39.0
84-85	34.58728390896432	35.0	34.0	36.0	31.0	37.5
86-87	34.37823994712328	35.0	34.0	36.0	31.0	37.0
88-89	34.22227924491153	35.0	34.0	35.5	31.0	37.0
90-91	34.07876366905336	35.0	34.0	35.0	31.0	36.0
92-93	33.99419714668579	35.0	34.0	35.0	31.0	36.0
94-95	33.9133469112254	35.0	34.0	35.0	31.0	36.0
96-97	33.87305690392364	35.0	34.0	35.0	31.0	36.0
98-99	33.89805110634791	35.0	34.0	35.0	31.0	35.5
100-101	32.67963199062475	34.0	32.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	7.0
20	17.0
21	99.0
22	420.0
23	1416.0
24	3718.0
25	8229.0
26	15640.0
27	26447.0
28	40426.0
29	58413.0
30	79634.0
31	107301.0
32	145617.0
33	204285.0
34	313747.0
35	511834.0
36	1017078.0
37	2237801.0
38	3224172.0
39	1485861.0
40	24466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15719976479573	20.26614057768105	13.24028598988285	24.336373667640373
2	14.777639523140365	21.619350656222146	41.016143183697494	22.58686663693999
3	18.199641959908682	26.404425828285927	30.505644843501052	24.890287368304342
4	12.227575563867184	17.928356713690356	39.64367172499773	30.20039599744473
5	12.882162399151916	38.15009657474692	36.16308246479876	12.804658561302402
6	29.596786663623178	38.51745571248697	19.108106658195386	12.777650965694468
7	26.175100955859225	32.64143024394554	23.887497462297365	17.295971337897868
8	24.210615007839834	36.899334334604944	21.817955945872427	17.072094711682794
9	25.720439169909838	16.37346210006765	21.41320957936815	36.49288915065437
10-11	23.9528842968291	26.807393782889477	29.628852388107596	19.610869532173826
12-13	24.681899943197358	24.376796120589905	29.573154440073214	21.368149496139523
14-15	21.815286515328975	25.79844751349862	26.97265027040892	25.41361570076348
16-17	21.60682392119328	28.901049692426202	27.625785265427368	21.86634112095315
18-19	21.568526386321967	28.34645926053712	29.060939575853904	21.02407477728701
20-21	22.399055996680104	27.615600038816275	28.772992921290015	21.21235104321361
22-23	22.0662062405329	27.575376636618287	28.624298828865825	21.73411829398299
24-25	21.838987878472857	27.61069171682668	28.773401664450386	21.776918740250075
26-27	21.852669364047593	27.81168069580513	28.611474734657143	21.724175205490138
28-29	21.752320351386263	27.994963314674397	28.573067825526277	21.679648508413056
30-31	22.057413567669833	27.7735957927449	28.499823045069974	21.669167594515294
32-33	21.811770823607453	27.92736094517421	28.46738492472732	21.79348330649101
34-35	21.935291372340508	27.962104786704817	28.48502461828424	21.617579222670432
36-37	21.95192262358518	27.887363289975582	28.47843787157407	21.68227621486517
38-39	21.749225393618595	28.000217728072172	28.545264745544852	21.705292132764377
40-41	22.001804269328794	27.872966875338857	28.429392841797014	21.695836013535335
42-43	21.89131029822116	27.93967010103437	28.286441954468433	21.882577646276033
44-45	21.996815301457367	28.06636189610994	28.10927293103827	21.827549871394424
46-47	22.023692954221367	28.01332746215518	27.933690487316206	22.02928909630725
48-49	21.99387704240845	28.05585047171974	27.99729772274856	21.952974763123255
50-51	21.960924316358295	28.166300991486725	27.891235709314927	21.981538982840057
52-53	22.148114036690377	28.0695175587524	27.75508238397934	22.027286020577886
54-55	22.070088474038226	28.000710841453873	27.862524296430518	22.066676388077386
56-57	22.108004175854077	28.092600811998036	27.765817057425124	22.03357795472276
58-59	22.07420112297283	28.11338098535709	27.791866640887143	22.020551250782933
60-61	22.123361429020218	28.094601358646376	27.801301939225105	21.9807352731083
62-63	22.109604225337627	28.129955282020003	27.734151673578516	22.026288819063854
64-65	22.042447510031494	28.096979163499768	27.78997882309434	22.070594503374398
66-67	22.133051907481587	28.133906223948962	27.59018436537806	22.14285750319139
68-69	22.102944438493967	28.100571443754042	27.6735997277641	22.122884389987895
70-71	22.091356053990978	27.890788657637543	27.7386935648816	22.279161723489878
72-73	22.134494408353994	27.97591772880242	27.696702420416493	22.192885442427094
74-75	22.047260811696713	28.066128539689387	27.660605943193	22.226004705420895
76-77	22.145166541401252	28.0210338377884	27.595969152790317	22.237830468020025
78-79	22.13975050630542	28.036590139382906	27.580258343402715	22.24340101090897
80-81	22.12067953213501	28.060354120125247	27.591390491375282	22.227575856364464
82-83	22.21718350623506	27.97496670751198	27.684588350235224	22.12326143601773
84-85	22.297547610524116	27.96924145704331	27.60251370807385	22.130697224358727
86-87	22.11897456969037	27.96985659578228	27.684290905904046	22.226877928623303
88-89	22.19685933380276	27.953081594585317	27.63660519693233	22.2134538746796
90-91	22.237390778811157	27.975720578964662	27.643211387162076	22.1436772550621
92-93	22.244710828151053	27.96979080136709	27.660101209245518	22.125397161236343
94-95	22.0770892576894	28.027164138877787	27.717538155429907	22.178208448002906
96-97	22.137357908193923	28.018055825107723	27.722661219530504	22.12192504716785
98-99	22.393235721388397	28.58965694043069	27.786169440042265	21.230937898138652
100-101	26.405844074479372	35.652227030097485	28.36877981137907	9.573149084044074
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1467.0
1	1323.0
2	1923.5
3	2855.0
4	4015.5
5	6666.5
6	8826.5
7	10087.5
8	10624.0
9	10148.0
10	9134.5
11	8190.0
12	8886.5
13	11771.5
14	18286.5
15	27106.0
16	34279.0
17	38595.0
18	40216.5
19	39442.0
20	37721.5
21	36773.5
22	38222.0
23	43750.5
24	53874.0
25	68747.5
26	87892.0
27	109218.5
28	133021.5
29	158343.5
30	180613.5
31	202189.5
32	225659.5
33	247976.5
34	270035.0
35	291402.0
36	313193.5
37	334342.0
38	350765.5
39	364439.5
40	379557.0
41	392901.5
42	401371.0
43	409861.0
44	418092.0
45	424596.5
46	428112.5
47	427195.5
48	422939.5
49	414059.0
50	401715.0
51	385064.5
52	362991.5
53	340901.0
54	318648.5
55	292321.0
56	264723.5
57	236720.5
58	208316.0
59	180832.0
60	152569.5
61	124743.0
62	99145.5
63	76828.5
64	58035.5
65	43526.0
66	32804.0
67	25233.0
68	19435.5
69	13927.5
70	9837.5
71	7178.0
72	5077.0
73	3478.0
74	2438.5
75	1668.5
76	1136.5
77	772.5
78	494.5
79	324.0
80	227.0
81	161.5
82	125.0
83	93.5
84	61.0
85	37.0
86	26.5
87	22.0
88	16.0
89	12.0
90	9.5
91	8.0
92	6.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.7882256909125524E-4
3	0.004544197049848369
4	3.1556923957280335E-5
5	4.207589860970712E-5
6	0.0
7	0.0
8	5.469866819261926E-4
9	0.002535072891234854
10-11	7.941825862582218E-4
12-13	3.155692395728034E-4
14-15	9.99302591980544E-5
16-17	0.0013779856794679081
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	6.909369796380872E-4
32-33	6.982746321534202E-4
34-35	5.841074349867184E-5
36-37	7.635322475442026E-4
38-39	6.137697300721486E-4
40-41	2.1736870509060662E-5
42-43	1.0422123997055695E-4
44-45	9.647820185923475E-4
46-47	0.0
48-49	4.4878688360683766E-4
50-51	3.448228498470624E-5
52-53	2.3237268721642907E-5
54-55	8.104372573276301E-4
56-57	0.0010160270241798388
58-59	1.0223594830036345E-4
60-61	1.2782832339640584E-4
62-63	1.2317474277264498E-5
64-65	0.0
66-67	0.0
68-69	6.3869155094108E-6
70-71	4.27049827850391E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	2.8591282980725664E-4
80-81	0.0
82-83	0.0
84-85	1.4177708502343433E-5
86-87	2.6578880694372537E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.5270186480280807E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	206.0
20-21	588.0
22-23	1179.0
24-25	2872.0
26-27	4932.0
28-29	10898.0
30-31	26537.0
32-33	33319.0
34-35	45245.0
36-37	72283.0
38-39	86769.0
40-41	84448.0
42-43	93291.0
44-45	108275.0
46-47	108351.0
48-49	103098.0
50-51	94989.0
52-53	91340.0
54-55	97839.0
56-57	100958.0
58-59	100130.0
60-61	97229.0
62-63	92766.0
64-65	95038.0
66-67	100525.0
68-69	100842.0
70-71	99668.0
72-73	93707.0
74-75	95216.0
76-77	94490.0
78-79	99327.0
80-81	99504.0
82-83	94397.0
84-85	92468.0
86-87	95260.0
88-89	101143.0
90-91	106979.0
92-93	104567.0
94-95	114095.0
96-97	170403.0
98-99	755919.0
100-101	5535540.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.02140279395898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.0461980042862	77.45900268591288
2	7.049317265961822	12.12784319915423
3	1.6703746516967481	4.310639121912748
4	0.609062444700046	2.0956962352886412
5	0.2690936316989046	1.157390584083036
6	0.12374737256263119	0.6386953547942523
7	0.06965835745736375	0.4194476737363824
8	0.04599974763265423	0.31655702556232085
9	0.028478956490111573	0.2204819808648768
>10	0.08635598410329277	1.1159512996443968
>50	0.0013830694968702624	0.07411642585683728
>100	2.934926370726797E-4	0.038797178491600276
>500	3.7021276289149054E-5	0.02538123469780904
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.103794930485356E-5	0.0	0.0	0.0
3	0.0	2.103794930485356E-5	0.0	0.0	0.0
4	0.0	6.311384791456068E-5	0.0	1.051897465242678E-5	0.0
5	1.051897465242678E-5	6.311384791456068E-5	0.0	2.103794930485356E-5	0.0
6	7.363282256698745E-5	6.311384791456068E-5	0.0	2.103794930485356E-5	0.0
7	7.363282256698745E-5	6.311384791456068E-5	0.0	2.103794930485356E-5	0.0
8	7.363282256698745E-5	6.311384791456068E-5	0.0	3.155692395728034E-5	3.155692395728034E-5
9	7.363282256698745E-5	1.472656451339749E-4	0.0	4.207589860970712E-5	5.25948732621339E-5
10-11	7.363282256698745E-5	1.472656451339749E-4	0.0	5.25948732621339E-5	5.25948732621339E-5
12-13	7.363282256698745E-5	1.472656451339749E-4	0.0	1.104492338504812E-4	5.25948732621339E-5
14-15	7.363282256698745E-5	2.4193641700581594E-4	0.0	1.2622769582912137E-4	7.889230989320084E-5
16-17	1.1570872117669459E-4	2.5245539165824274E-4	0.0	1.2622769582912137E-4	8.415179721941424E-5
18-19	1.1570872117669459E-4	2.84012315615523E-4	0.0	1.472656451339749E-4	8.415179721941424E-5
20-21	1.1570872117669459E-4	3.260882142252302E-4	0.0	1.7882256909125527E-4	8.941128454562764E-5
22-23	1.1570872117669459E-4	3.734236001611507E-4	0.0	1.998605183961088E-4	1.2096820850290797E-4
24-25	1.1570872117669459E-4	4.365374480757114E-4	1.051897465242678E-5	2.103794930485356E-4	1.472656451339749E-4
26-27	1.1570872117669459E-4	4.680943720329917E-4	1.051897465242678E-5	2.6297436631066953E-4	1.472656451339749E-4
28-29	1.1570872117669459E-4	4.733538593592051E-4	1.051897465242678E-5	6.521764284504604E-4	1.472656451339749E-4
30-31	1.1570872117669459E-4	5.575056565786193E-4	1.051897465242678E-5	0.0019880862093086616	1.472656451339749E-4
32-33	1.2622769582912137E-4	5.680246312310461E-4	1.051897465242678E-5	0.004907101675357093	1.6830359443882847E-4
34-35	1.3674667048154814E-4	6.574359157766737E-4	1.051897465242678E-5	0.0087202299868618	1.7882256909125527E-4
36-37	1.420061578077615E-4	7.678851496271549E-4	1.051897465242678E-5	0.014079647572273247	1.7882256909125527E-4
38-39	1.577846197864017E-4	7.994420735844352E-4	1.051897465242678E-5	0.02235282113640691	1.7882256909125527E-4
40-41	1.577846197864017E-4	9.098913074349164E-4	1.051897465242678E-5	0.03216176499979488	1.7882256909125527E-4
42-43	1.577846197864017E-4	9.3092925673977E-4	1.051897465242678E-5	0.04106607704307415	1.7882256909125527E-4
44-45	1.8934154374368204E-4	0.001009821566632971	1.051897465242678E-5	0.05070145782469708	1.7882256909125527E-4
46-47	1.998605183961088E-4	0.001067675927221318	1.051897465242678E-5	0.0614728878687821	1.7882256909125527E-4
48-49	1.998605183961088E-4	0.0010834543891999585	1.051897465242678E-5	0.07134494558008464	1.8934154374368204E-4
50-51	2.2615795502717577E-4	0.0011676061864193724	1.051897465242678E-5	0.08086461764053088	1.8934154374368204E-4
52-53	2.4193641700581594E-4	0.0011833846483980128	1.051897465242678E-5	0.09036325175167226	1.9460103106989544E-4
54-55	2.5245539165824274E-4	0.0012359795216601466	1.051897465242678E-5	0.09970410124302723	2.051200057223222E-4
56-57	2.629743663106695E-4	0.0012833149075960671	1.051897465242678E-5	0.10819817327486186	2.208984677009624E-4
58-59	2.6823385363688285E-4	0.0012990933695747075	1.051897465242678E-5	0.11612422067546543	2.3141744235338917E-4
60-61	3.1030975224659004E-4	0.0013411692681844145	1.051897465242678E-5	0.12374521781114864	2.5245539165824274E-4
62-63	3.4712616353008374E-4	0.0013674667048154815	1.051897465242678E-5	0.13088760160014642	2.5245539165824274E-4
64-65	3.4712616353008374E-4	0.0015568082485591635	1.051897465242678E-5	0.13793005512994616	2.5245539165824274E-4
66-67	3.5764513818251054E-4	0.0015883651725164438	1.051897465242678E-5	0.1449409517357886	2.577148789844561E-4
68-69	3.5764513818251054E-4	0.0016094031218212973	1.051897465242678E-5	0.15201496218954563	2.629743663106695E-4
70-71	3.5764513818251054E-4	0.0016514790204310045	1.051897465242678E-5	0.15852094801207156	2.734933409630963E-4
72-73	3.786830874873641E-4	0.001672516969735858	1.051897465242678E-5	0.16401711226796456	3.260882142252302E-4
74-75	3.786830874873641E-4	0.0017093333810193519	1.051897465242678E-5	0.16856656880513915	3.260882142252302E-4
76-77	3.786830874873641E-4	0.0017829662035863392	1.051897465242678E-5	0.17258481712236617	3.260882142252302E-4
78-79	3.786830874873641E-4	0.0017987446655649793	1.051897465242678E-5	0.17610341414360295	3.260882142252302E-4
80-81	3.786830874873641E-4	0.0018040041528911929	1.051897465242678E-5	0.17777067162601257	3.418666762038704E-4
82-83	3.786830874873641E-4	0.0018250421021960464	1.051897465242678E-5	0.1784912213897038	3.681641128349373E-4
84-85	3.786830874873641E-4	0.0018303015895222597	1.051897465242678E-5	0.17904346755895623	3.681641128349373E-4
86-87	3.892020621397909E-4	0.0018355610768484732	1.051897465242678E-5	0.17932222038724555	3.681641128349373E-4
88-89	3.892020621397909E-4	0.0019407508233727408	1.051897465242678E-5	0.17940111269713874	3.681641128349373E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9075	0.0	42.15473	1
GTATCAA	15620	0.0	35.27598	1
TCAACGC	17840	0.0	30.096287	4
ATCAACG	17915	0.0	29.92444	3
CAACGCA	18170	0.0	29.549683	5
AACGCAG	18940	0.0	28.392313	6
TATCAAC	19680	0.0	27.619019	2
ACGCAGA	22330	0.0	24.119135	7
CGCAGAG	22790	0.0	23.577688	8
TACATGG	24440	0.0	21.968233	2
GTACATG	25075	0.0	21.924868	1
ACATGGG	24920	0.0	20.746763	3
GCAGAGT	28095	0.0	19.06659	9
CATGGGG	17815	0.0	18.795815	4
AGAGTAC	24790	0.0	17.389875	10-11
GAGTACT	15555	0.0	17.11139	12-13
CAGAGTA	27360	0.0	16.613218	10-11
GTACTTT	18090	0.0	14.737003	14-15
AGTACTT	17635	0.0	14.56377	12-13
TATACTG	5525	0.0	14.419242	5
>>END_MODULE