FastQCFastQC Report
Fri 27 May 2016
SRR2140012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8758163
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87250.043.8712581
GTATCAA149650.034.7578351
TCAACGC172000.029.3758894
ATCAACG172700.029.2328593
CAACGCA175550.028.7820075
AACGCAG182850.027.725976
TATCAAC187950.027.290282
GTACATG247550.024.3450091
ACGCAGA213300.023.6682057
TACATGG248450.023.263952
CGCAGAG220550.022.8901758
ACATGGG252200.022.4800873
CATGGGG200650.020.0094814
GCAGAGT270850.018.6235039
GAGTACT142650.017.76908712-13
AGAGTAC235700.017.13285410-11
CAGAGTA261300.016.75642210-11
ATGGGGG132350.016.5175135
GAGTACA178150.016.2101461
GTACTTT163550.015.5378314-15
Produced by FastQC (version 0.11.5)