##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140012_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8758163
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68882093196941	34.0	31.0	34.0	31.0	34.0
2	32.86998894631214	34.0	31.0	34.0	31.0	34.0
3	32.966135021693475	34.0	31.0	34.0	31.0	34.0
4	36.371699978637075	37.0	37.0	37.0	35.0	37.0
5	36.20625866406003	37.0	37.0	37.0	35.0	37.0
6	36.259951316274886	37.0	37.0	37.0	35.0	37.0
7	36.25661579945475	37.0	37.0	37.0	35.0	37.0
8	36.23847478061324	37.0	37.0	37.0	35.0	37.0
9	38.12504574303995	39.0	38.0	39.0	37.0	39.0
10-11	38.015679886295786	39.0	38.0	39.0	36.0	39.0
12-13	38.051702623027225	39.0	38.0	39.0	36.0	39.0
14-15	39.46802531535437	40.5	39.0	41.0	37.0	41.0
16-17	39.448186166436955	40.0	39.0	41.0	37.0	41.0
18-19	39.487176020816236	40.5	39.0	41.0	37.0	41.0
20-21	39.50079052062429	41.0	39.0	41.0	37.0	41.0
22-23	39.436346733518725	40.0	39.0	41.0	37.0	41.0
24-25	39.391372107729765	41.0	39.0	41.0	36.5	41.0
26-27	39.345941661931974	40.0	39.0	41.0	36.0	41.0
28-29	39.24805022123142	40.0	39.0	41.0	36.0	41.0
30-31	39.15054229218356	40.0	39.0	41.0	36.0	41.0
32-33	39.04389644198397	40.0	39.0	41.0	35.5	41.0
34-35	38.96758723148407	40.0	38.5	41.0	35.0	41.0
36-37	38.96672591939625	40.0	38.0	41.0	35.0	41.0
38-39	38.959340656474524	40.0	38.0	41.0	35.0	41.0
40-41	38.90275965168857	40.0	38.0	41.0	35.0	41.0
42-43	38.85831081439018	40.0	38.0	41.0	35.0	41.0
44-45	38.8130295933187	40.0	38.0	41.0	35.0	41.0
46-47	38.727312030114454	40.0	38.0	41.0	35.0	41.0
48-49	38.68558999039834	40.0	38.0	41.0	35.0	41.0
50-51	38.57303123210088	40.0	38.0	41.0	35.0	41.0
52-53	38.383702319068334	40.0	38.0	41.0	34.0	41.0
54-55	38.22331093843673	40.0	37.0	41.0	34.0	41.0
56-57	38.03955947592348	40.0	37.0	41.0	34.0	41.0
58-59	37.823930204212076	39.0	36.5	41.0	33.5	41.0
60-61	37.66883639543252	39.0	36.0	41.0	33.0	41.0
62-63	37.4731237955186	39.0	35.5	41.0	33.0	41.0
64-65	37.19158399794932	38.5	35.0	40.0	33.0	41.0
66-67	36.90051143251205	38.0	35.0	40.0	33.0	41.0
68-69	36.630468193888795	37.0	35.0	40.0	32.0	41.0
70-71	36.32666836167863	37.0	35.0	39.0	32.0	41.0
72-73	35.97606276910719	36.0	35.0	39.0	32.0	41.0
74-75	35.60190362376314	36.0	34.5	39.0	31.5	40.0
76-77	33.711198174175934	34.5	32.0	36.5	29.0	39.0
78-79	34.79402466905859	35.0	34.0	37.0	31.0	39.0
80-81	34.74790627109681	35.0	34.0	36.5	31.0	39.0
82-83	34.50105086040415	35.0	34.0	36.0	31.0	38.0
84-85	34.283527273030856	35.0	34.0	36.0	31.0	37.0
86-87	34.10496634875365	35.0	34.0	35.5	31.0	37.0
88-89	33.97705098760312	35.0	34.0	35.0	31.0	36.5
90-91	33.85779116011177	35.0	34.0	35.0	31.0	36.0
92-93	33.789837986627006	35.0	34.0	35.0	31.0	36.0
94-95	33.72162531581469	35.0	34.0	35.0	31.0	36.0
96-97	33.696789800014656	35.0	34.0	35.0	31.0	35.0
98-99	33.73898048541872	35.0	34.0	35.0	31.0	35.0
100-101	32.45302007292325	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	14.0
20	23.0
21	129.0
22	504.0
23	1689.0
24	4423.0
25	9707.0
26	18245.0
27	30669.0
28	45956.0
29	64818.0
30	88147.0
31	116725.0
32	156542.0
33	218823.0
34	340361.0
35	559465.0
36	1103496.0
37	2243197.0
38	2667631.0
39	1068904.0
40	18693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.273298293260815	19.34417068967545	12.841928153198337	24.540602863865402
2	15.443749789481675	20.781946405317562	40.07079124631986	23.703512558880906
3	18.913083790299083	25.383842093099517	29.598055120893818	26.105018995707578
4	12.89220566877061	16.988785315637074	37.92999899522274	32.18901002036957
5	13.596665051029582	37.56357360654156	35.29939810382438	13.540363238604478
6	31.438601907728824	36.937346336212286	18.18432701012758	13.439724745931311
7	27.631502176883437	31.83137833812867	22.69056878708469	17.8465506979032
8	25.44675362666013	36.16792722918953	20.750281424327373	17.63503771982297
9	26.15724755239134	16.097365863372374	20.483228011714175	37.26215857252211
10-11	25.083959487527707	26.120081163787596	28.415415319293825	20.380544029390872
12-13	25.616118403249637	23.54681440278744	28.681958425409604	22.155108768553315
14-15	22.640461892092617	24.93752818446408	25.93659767968108	26.485412243762223
16-17	22.588606878777316	28.09401168457916	26.558074244702496	22.759307191941026
18-19	22.599956178025003	27.477240375635848	28.039002014463538	21.88380143187561
20-21	23.240304329427744	26.732822722581883	27.872333914676588	22.154539033313778
22-23	23.062907402724502	26.656099015801633	27.73379157572513	22.547202005748737
24-25	22.82239912028632	26.738406473771686	27.812253585600764	22.626940820341233
26-27	22.78097781206313	26.929482430760665	27.695550302055917	22.59398945512028
28-29	22.73471788880992	26.989795025993995	27.710204299535064	22.565282785661022
30-31	22.9539020530018	26.844625578700427	27.60192336100198	22.599549007295792
32-33	22.69675174020576	27.05923081313622	27.627993929751586	22.616023516906434
34-35	22.837255946565687	27.025581880039027	27.645413274716617	22.49174889867867
36-37	22.86369522089558	26.976411325447415	27.55969317093211	22.6002002827249
38-39	22.741661411742385	27.040288295376968	27.67139656355854	22.546653729322106
40-41	22.887138253179288	26.928355667180714	27.57911688027606	22.605389199363934
42-43	22.80521196268407	27.033418901842943	27.434209808480865	22.727159326992123
44-45	22.889112231153533	27.089497353798652	27.28938114602658	22.73200926902123
46-47	22.94397104490106	27.13647213420761	27.05216314084582	22.86739368004551
48-49	22.86116778493279	27.21156162544302	27.072060203948105	22.855210385676084
50-51	22.84261596851064	27.314111269143094	27.019047021233273	22.824225741112997
52-53	23.055387265697426	27.156942128077922	26.87160819229881	22.91606241392584
54-55	22.939283822250015	27.115531358308587	26.963479518364174	22.981705301077223
56-57	22.9542055664482	27.224150919852285	26.93849625821199	22.883147255487522
58-59	22.94838644857374	27.237700962782135	26.926869381129958	22.887043207514168
60-61	22.978905065547345	27.22495187388167	26.956552637023947	22.83959042354704
62-63	23.013961557212152	27.28473914266604	26.837963872006583	22.86333542811522
64-65	22.97586426886854	27.27325851980066	26.859805680041415	22.891071531289384
66-67	23.00006410119724	27.26662922699039	26.74099940962203	22.992307262190334
68-69	22.90830594692054	27.21577766927211	26.882390720590944	22.993525663216406
70-71	22.930667434297675	27.092392521317137	26.873973801624217	23.102966242760967
72-73	22.974153834071554	27.12838467053106	26.81127270456503	23.086188790832356
74-75	22.782710643418692	27.2582014229134	26.887995435480704	23.071092498187195
76-77	23.005900069422815	27.170413004859594	26.77670386036096	23.04698306535662
78-79	22.936055071828815	27.143886816243256	26.788523879086558	23.13153423284137
80-81	22.927171079634952	27.22591558821202	26.80854040440555	23.038372927747478
82-83	22.988847020528905	27.151914231353857	26.911803318557297	22.947435429559942
84-85	22.99897571341524	27.21089747743039	26.820110669542725	22.970016139611644
86-87	22.89718439240263	27.191712830715964	26.910484114936096	23.00061866194531
88-89	22.91332962612454	27.183949211587265	26.85959203764275	23.043129124645446
90-91	22.99536238226719	27.181722444706153	26.87379380900421	22.94912136402245
92-93	23.03477741713352	27.148707549886662	26.877283799194355	22.939231233785463
94-95	22.8485063679817	27.20296737380581	26.971603587700375	22.976922670512113
96-97	22.921326783273045	27.187915111889044	26.944497433129705	22.946260671708202
98-99	23.191234723978088	27.80297040897084	26.947319951075617	22.058474915975456
100-101	24.03242221082235	30.28267170585371	23.921197442156004	21.76370864116794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1385.0
1	1258.5
2	1842.0
3	2757.0
4	3750.5
5	6103.0
6	8096.0
7	9364.0
8	10169.5
9	9860.5
10	8662.0
11	7420.5
12	7814.5
13	10252.5
14	16040.5
15	23886.5
16	30234.5
17	34016.5
18	35089.5
19	33725.5
20	31088.0
21	28739.0
22	28423.0
23	31406.5
24	37629.0
25	47442.5
26	59872.0
27	73661.0
28	90137.0
29	108373.5
30	125735.0
31	143570.5
32	162252.5
33	179468.5
34	194416.5
35	209449.5
36	227427.0
37	245277.0
38	262574.5
39	277061.0
40	288727.0
41	302516.0
42	315070.5
43	327311.0
44	341980.5
45	357837.5
46	370277.5
47	378391.0
48	384139.0
49	384692.0
50	382479.5
51	379136.5
52	372038.0
53	362254.0
54	349546.5
55	330426.0
56	307880.5
57	281989.5
58	252357.0
59	221005.0
60	186311.5
61	153404.5
62	124364.5
63	97542.5
64	73806.5
65	55365.5
66	42226.5
67	32419.0
68	24999.5
69	18397.0
70	13213.0
71	9635.0
72	6773.5
73	4701.5
74	3279.0
75	2169.5
76	1440.0
77	932.0
78	594.5
79	410.0
80	309.0
81	228.5
82	173.5
83	117.5
84	75.5
85	61.0
86	50.5
87	41.0
88	28.0
89	16.5
90	8.0
91	4.0
92	3.5
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.055225507906167E-4
3	0.0045100781979052
4	3.425375846510278E-5
5	9.134335590694076E-5
6	0.0
7	0.0
8	4.110451015812334E-4
9	0.002591867723859444
10-11	7.992543641857316E-4
12-13	2.8544798720918986E-4
14-15	1.5414191309296254E-4
16-17	0.0014158220165575819
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	5.265502971432072E-4
32-33	6.309989717584938E-4
34-35	2.301040398158224E-5
36-37	7.567154597950433E-4
38-39	5.754806527764239E-4
40-41	2.9275053649463317E-5
42-43	9.436610190324043E-5
44-45	9.695783779500983E-4
46-47	0.0
48-49	5.21724707832647E-4
50-51	1.2247902194621983E-5
52-53	1.2355326081457057E-5
54-55	5.234115114890696E-4
56-57	7.673081446614851E-4
58-59	1.0161412771701888E-4
60-61	1.0903486633576082E-4
62-63	1.2952070990819184E-5
64-65	1.96097874540894E-5
66-67	6.601564676453354E-6
68-69	0.0
70-71	4.6537862901749056E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	2.954548907914308E-4
80-81	0.0
82-83	0.0
84-85	2.9119723851834768E-5
86-87	3.0185723183666135E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	2.330885877263475E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	191.0
20-21	523.0
22-23	1115.0
24-25	2820.0
26-27	4162.0
28-29	8948.0
30-31	18751.0
32-33	23092.0
34-35	31257.0
36-37	50268.0
38-39	62227.0
40-41	61151.0
42-43	68365.0
44-45	81268.0
46-47	82166.0
48-49	79031.0
50-51	72174.0
52-53	68519.0
54-55	73259.0
56-57	76684.0
58-59	77194.0
60-61	76123.0
62-63	71650.0
64-65	73703.0
66-67	78534.0
68-69	81285.0
70-71	80403.0
72-73	75193.0
74-75	75698.0
76-77	74776.0
78-79	80688.0
80-81	82446.0
82-83	77288.0
84-85	76403.0
86-87	79508.0
88-89	85645.0
90-91	92876.0
92-93	92884.0
94-95	103329.0
96-97	162614.0
98-99	750015.0
100-101	5443937.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.15257828218823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.86051599197931	72.17967917944532
2	8.076971311910285	13.270880359693965
3	2.172882809328029	5.35523775274026
4	0.8604825947739122	2.827634549104971
5	0.4140322882570645	1.7006909986196004
6	0.20620347835970348	1.0164088438803027
7	0.1252509136172078	0.7202779840097281
8	0.07671424656052471	0.5041818516738076
9	0.05129432372538222	0.3792564850753231
>10	0.15278840359528023	1.8598870981103914
>50	0.0024975114907564563	0.13275137160435016
>100	3.6612640252198513E-4	0.05311352604198702
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.425375846510278E-5	0.0	0.0	0.0
3	0.0	3.425375846510278E-5	0.0	2.283583897673519E-5	0.0
4	0.0	7.992543641857316E-5	0.0	4.567167795347038E-5	0.0
5	0.0	7.992543641857316E-5	0.0	4.567167795347038E-5	0.0
6	0.0	7.992543641857316E-5	0.0	9.134335590694076E-5	0.0
7	0.0	7.992543641857316E-5	0.0	9.134335590694076E-5	0.0
8	0.0	7.992543641857316E-5	0.0	1.0276127539530835E-4	0.0
9	0.0	1.5985087283714632E-4	0.0	1.7126879232551393E-4	0.0
10-11	0.0	1.5985087283714632E-4	0.0	2.8544798720918986E-4	0.0
12-13	0.0	1.5985087283714632E-4	0.0	3.4824654439521166E-4	0.0
14-15	0.0	2.626121482324547E-4	0.0	3.653734236277631E-4	0.0
16-17	0.0	2.7403006772082225E-4	0.0	3.996271820928658E-4	0.0
18-19	0.0	2.9115694695337367E-4	0.0	4.852615782556228E-4	0.0
20-21	0.0	3.082838261859251E-4	0.0	6.336945316044015E-4	0.0
22-23	0.0	3.5395550413939547E-4	0.0	7.478737264880774E-4	0.0
24-25	0.0	4.167540613254172E-4	0.0	7.878364446973641E-4	0.0
26-27	0.0	4.395899003021524E-4	0.0	8.73470840860121E-4	0.0
28-29	5.708959744183797E-6	4.5100781979052E-4	0.0	0.0011246650696042082	0.0
30-31	1.1417919488367594E-5	5.138063769765418E-4	0.0	0.0020095538299526967	0.0
32-33	1.1417919488367594E-5	5.138063769765418E-4	0.0	0.003585226719347425	0.0
34-35	1.1417919488367594E-5	6.108586926276663E-4	0.0	0.005617616388276857	0.0
36-37	1.1417919488367594E-5	7.079110082787909E-4	0.0	0.008654782972182636	0.0
38-39	1.1417919488367594E-5	7.250378875113422E-4	0.0	0.01347314499627376	0.0
40-41	1.1417919488367594E-5	8.106722836740992E-4	0.0	0.01894232843120184	0.0
42-43	1.1417919488367594E-5	8.44926042139202E-4	0.0	0.02394908612685103	0.0
44-45	1.1417919488367594E-5	9.876500357437969E-4	0.0	0.02928696348766288	0.0
46-47	1.1417919488367594E-5	0.0011075381903716567	0.0	0.03546405793086975	0.0
48-49	1.1417919488367594E-5	0.0011075381903716567	0.0	0.041829548045634685	0.0
50-51	1.1417919488367594E-5	0.0012274263449995166	0.0	0.04775544826009746	0.0
52-53	1.1417919488367594E-5	0.0012331353047437003	0.0	0.05331597505093248	0.0
54-55	1.1417919488367594E-5	0.001238844264487884	0.0	0.058847957043046586	0.0
56-57	1.1417919488367594E-5	0.0012445532242320678	0.0	0.06496796188881163	5.708959744183797E-6
58-59	1.1417919488367594E-5	0.0012559711437204353	0.0	0.0709281158617395	1.1417919488367594E-5
60-61	1.1417919488367594E-5	0.0012559711437204353	0.0	0.07636875449794667	1.1417919488367594E-5
62-63	1.1417919488367594E-5	0.0012788069826971706	0.0	0.08174088561722362	1.1417919488367594E-5
64-65	1.1417919488367594E-5	0.001495747452976155	0.0	0.08731853928729119	1.1417919488367594E-5
66-67	1.1417919488367594E-5	0.0015071653724645225	0.0	0.09299324527300988	1.1417919488367594E-5
68-69	1.1417919488367594E-5	0.001552837050417993	0.0	0.09874216773540297	1.1417919488367594E-5
70-71	1.1417919488367594E-5	0.0015927997686272794	0.0	0.10442829164061002	1.1417919488367594E-5
72-73	1.1417919488367594E-5	0.0016042176881156472	0.0	0.10902400423467798	1.1417919488367594E-5
74-75	1.1417919488367594E-5	0.0016156356076040147	0.0	0.11283758934379276	1.1417919488367594E-5
76-77	1.1417919488367594E-5	0.0016727252050458526	0.0	0.11631434582800068	1.1417919488367594E-5
78-79	1.1417919488367594E-5	0.0017126879232551393	0.0	0.11963125143937148	1.1417919488367594E-5
80-81	1.1417919488367594E-5	0.0017126879232551393	0.0	0.12088722258309191	1.1417919488367594E-5
82-83	1.1417919488367594E-5	0.0017126879232551393	0.0	0.12142957375878938	1.1417919488367594E-5
84-85	1.1417919488367594E-5	0.0017126879232551393	0.0	0.12200046973320774	1.1417919488367594E-5
86-87	1.1417919488367594E-5	0.0017126879232551393	0.0	0.12224024604246347	1.1417919488367594E-5
88-89	1.1417919488367594E-5	0.001781195440185345	0.0	0.12228591772041694	1.1417919488367594E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8725	0.0	43.871258	1
GTATCAA	14965	0.0	34.757835	1
TCAACGC	17200	0.0	29.375889	4
ATCAACG	17270	0.0	29.232859	3
CAACGCA	17555	0.0	28.782007	5
AACGCAG	18285	0.0	27.72597	6
TATCAAC	18795	0.0	27.29028	2
GTACATG	24755	0.0	24.345009	1
ACGCAGA	21330	0.0	23.668205	7
TACATGG	24845	0.0	23.26395	2
CGCAGAG	22055	0.0	22.890175	8
ACATGGG	25220	0.0	22.480087	3
CATGGGG	20065	0.0	20.009481	4
GCAGAGT	27085	0.0	18.623503	9
GAGTACT	14265	0.0	17.769087	12-13
AGAGTAC	23570	0.0	17.132854	10-11
CAGAGTA	26130	0.0	16.756422	10-11
ATGGGGG	13235	0.0	16.517513	5
GAGTACA	17815	0.0	16.210146	1
GTACTTT	16355	0.0	15.53783	14-15
>>END_MODULE