FastQCFastQC Report
Fri 27 May 2016
SRR2140011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8768782
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85050.039.3832321
GTATCAA145800.033.6771431
TCAACGC163700.029.0389294
ATCAACG164500.028.9492913
CAACGCA168550.028.2287545
AACGCAG175450.027.118596
TATCAAC179600.026.7976322
ACGCAGA204950.023.1532977
CGCAGAG209650.022.6140678
GTACATG220900.021.749191
TACATGG216800.021.614292
ACATGGG218250.020.7345683
CATGGGG159100.019.2455964
GCAGAGT258200.018.4274069
AGAGTAC224900.017.4606510-11
GAGTACT148150.017.0142412-13
CAGAGTA252900.015.8870310-11
ATGGGGG106450.015.256455
GAGTACA157850.015.1646471
AGTACTT168450.014.87596912-13
Produced by FastQC (version 0.11.5)