##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140011_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8768782
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.693785864445026	34.0	31.0	34.0	31.0	34.0
2	32.8739925339688	34.0	31.0	34.0	31.0	34.0
3	32.97204868361421	34.0	31.0	34.0	31.0	34.0
4	36.37478603071669	37.0	37.0	37.0	35.0	37.0
5	36.215919953307086	37.0	37.0	37.0	35.0	37.0
6	36.270111858180535	37.0	37.0	37.0	35.0	37.0
7	36.26923077800315	37.0	37.0	37.0	35.0	37.0
8	36.25160198987727	37.0	37.0	37.0	35.0	37.0
9	38.14077428313305	39.0	39.0	39.0	37.0	39.0
10-11	38.03406676092529	39.0	38.0	39.0	36.0	39.0
12-13	38.07409854641158	39.0	38.0	39.0	37.0	39.0
14-15	39.49369747132498	41.0	39.0	41.0	37.0	41.0
16-17	39.47639700701876	40.0	39.0	41.0	37.0	41.0
18-19	39.5225655626973	41.0	39.0	41.0	37.0	41.0
20-21	39.53846126775527	41.0	39.0	41.0	37.0	41.0
22-23	39.473959024979244	41.0	39.0	41.0	37.0	41.0
24-25	39.43295181194	41.0	39.0	41.0	37.0	41.0
26-27	39.396733519547816	40.5	39.0	41.0	37.0	41.0
28-29	39.2992140665324	40.0	39.0	41.0	36.0	41.0
30-31	39.20387520670144	40.0	39.0	41.0	36.0	41.0
32-33	39.099362081102356	40.0	39.0	41.0	35.5	41.0
34-35	39.02732192013838	40.0	39.0	41.0	35.0	41.0
36-37	39.030114187877544	40.0	39.0	41.0	35.0	41.0
38-39	39.02725851065283	40.0	39.0	41.0	35.0	41.0
40-41	38.97890666887463	40.0	39.0	41.0	35.0	41.0
42-43	38.94181857129719	40.0	38.0	41.0	35.0	41.0
44-45	38.9072868870025	40.0	38.0	41.0	35.0	41.0
46-47	38.835401495407226	40.0	38.0	41.0	35.0	41.0
48-49	38.80927722491998	40.0	38.0	41.0	35.0	41.0
50-51	38.715576787118145	40.0	38.0	41.0	35.0	41.0
52-53	38.54590960339519	40.0	38.0	41.0	34.5	41.0
54-55	38.41274096491934	40.0	38.0	41.0	34.0	41.0
56-57	38.2533127700534	40.0	37.0	41.0	34.0	41.0
58-59	38.06636301434328	40.0	37.0	41.0	34.0	41.0
60-61	37.941905326931504	39.5	36.5	41.0	34.0	41.0
62-63	37.7767979198058	39.0	36.0	41.0	33.5	41.0
64-65	37.52676788732667	39.0	36.0	41.0	33.0	41.0
66-67	37.26235956589768	39.0	35.0	40.0	33.0	41.0
68-69	37.008592855840405	38.0	35.0	40.0	33.0	41.0
70-71	36.71478968036152	37.0	35.0	40.0	33.0	41.0
72-73	36.36335061324669	37.0	35.0	39.0	32.0	41.0
74-75	35.9835255657302	36.0	35.0	39.0	32.0	40.5
76-77	34.05330745620131	34.5	32.5	36.5	29.0	39.0
78-79	35.12329170372077	35.0	34.0	37.0	31.0	39.0
80-81	35.05131223161249	35.0	34.0	37.0	31.0	39.0
82-83	34.77227162488621	35.0	34.0	36.5	31.0	39.0
84-85	34.52337904044576	35.0	34.0	36.0	31.0	37.5
86-87	34.314593640660064	35.0	34.0	36.0	31.0	37.0
88-89	34.15948272118638	35.0	34.0	35.5	31.0	37.0
90-91	34.01406255850283	35.0	34.0	35.0	31.0	36.0
92-93	33.928037392503946	35.0	34.0	35.0	31.0	36.0
94-95	33.842989175022055	35.0	34.0	35.0	31.0	36.0
96-97	33.8050599023877	35.0	34.0	35.0	31.0	36.0
98-99	33.83455202334018	35.0	34.0	35.0	31.0	35.0
100-101	32.564591764667306	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	8.0
20	33.0
21	105.0
22	450.0
23	1440.0
24	3924.0
25	8608.0
26	16424.0
27	27534.0
28	41773.0
29	59820.0
30	81268.0
31	108838.0
32	146230.0
33	203574.0
34	311305.0
35	504568.0
36	987663.0
37	2103648.0
38	2861611.0
39	1274910.0
40	25045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.415993464086576	20.042019518788358	13.137970587021094	24.404016430103976
2	14.947584426911511	21.40619982191341	40.79014292543719	22.85607282573789
3	18.49680321948717	25.94401708051672	30.230625979588183	25.328553720407925
4	12.32206182482025	17.68087130974722	39.284194282075404	30.712872583357132
5	13.033477861230411	37.96625072130901	36.00410775501267	12.996163662447913
6	30.09883242621381	38.16506100847301	18.872062277292333	12.864044288020846
7	26.644852158486778	32.37243211200826	23.48577031564931	17.496945413855652
8	24.471529278135012	36.890911983413815	21.488559651352617	17.148999087098556
9	25.669091016085005	16.391854605636652	21.203181191665017	36.73587318661332
10-11	24.14538228858136	26.74175443702362	29.33191701042661	19.780946263968406
12-13	24.87161802007446	24.21502530462139	29.391712893302742	21.521643782001412
14-15	21.900451642410424	25.72489185631263	26.75334811647262	25.62130838480433
16-17	21.710465055345786	28.840272860873768	27.482588671487367	21.966673412293087
18-19	21.740259935758466	28.23235883843389	28.87170076756384	21.155680458243804
20-21	22.46756308190666	27.4701684362434	28.722238207507093	21.34003027434284
22-23	22.13989900101633	27.442790452485806	28.601819770065756	21.81549077643211
24-25	21.939874273830213	27.47675723377786	28.727978627246998	21.85538986514493
26-27	21.952838964734493	27.66627517766495	28.563740510804138	21.817145346796416
28-29	21.842800728259686	27.829984819359517	28.540431943173367	21.786782509207438
30-31	22.138243418833245	27.65541241193235	28.46932695310649	21.737017216127917
32-33	21.908067617685777	27.784073438268393	28.43891763385088	21.86894131019495
34-35	22.005395953787627	27.819136972721516	28.47040706349307	21.705060009997787
36-37	22.02443921438569	27.760537778654886	28.450544477446343	21.764478529513077
38-39	21.85302722113405	27.889333227305958	28.4872195157339	21.770420035826092
40-41	22.072528390930245	27.74129106146544	28.390409003338597	21.795771544265722
42-43	21.97350547648074	27.827152324447134	28.242474147365915	21.956868051706213
44-45	22.047697541773044	27.942739226388134	28.12124296289645	21.888320268942373
46-47	22.05147945323996	27.93680948585705	27.912111872188017	22.099599188714976
48-49	22.060796527823157	27.98042645407283	27.945388591115005	22.013388426989003
50-51	22.009108105388723	28.104133921796116	27.866618185381004	22.02013978743416
52-53	22.211918820566694	27.95430303693383	27.746191945558056	22.087586196941412
54-55	22.12020471775157	27.91351976708723	27.831453102646353	22.134822412514847
56-57	22.15196859184093	28.004596100348905	27.791410492858958	22.052024814951203
58-59	22.127176675157564	28.066835072597947	27.7416958557238	22.06429239652069
60-61	22.173066869001122	28.051419940472925	27.766136097424816	22.009377093101136
62-63	22.13572609288928	28.068817341537688	27.72363015025508	22.07182641531795
64-65	22.12183728247993	28.032219290692602	27.732642919961346	22.113300506866118
66-67	22.18542033910022	28.061613132223563	27.61521803287842	22.137748495797805
68-69	22.125827898703303	28.063185711661333	27.69247180760116	22.118514582034205
70-71	22.124394817452647	27.884366606822432	27.721275600817137	22.269962974907784
72-73	22.174774571317243	27.920078704906526	27.704913816449384	22.200232907326843
74-75	22.033530777680447	28.0374796646227	27.675954893699856	22.253034663996996
76-77	22.164478236069844	27.977564751769098	27.60855600280409	22.249401009356973
78-79	22.130425667547446	28.01112121573298	27.603996139320135	22.254456977399435
80-81	22.12112288126595	28.012010313764446	27.616897452706922	22.24996935226268
82-83	22.21991246834289	27.98290869564774	27.669066333362625	22.128112502646744
84-85	22.26699626073417	27.958664598471266	27.603257407756914	22.17108173303765
86-87	22.08755354714316	27.987331692858536	27.697004508333915	22.228110251664393
88-89	22.156959957254855	27.9834788695022	27.656626917472018	22.202934255770927
90-91	22.2382092389362	27.990355898776315	27.66573969890185	22.105695163385633
92-93	22.231718003737477	27.96902255840139	27.651767591149007	22.147491846712125
94-95	22.07797361850626	28.028536228299295	27.712805679788772	22.180684473405677
96-97	22.08990377318642	28.05839135877914	27.747282353385206	22.104422514649233
98-99	22.380976393393244	28.595754287180792	27.78191502062378	21.24135429880218
100-101	23.099441508090344	31.230747267229347	24.873606646231	20.796204578449313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1551.0
1	1331.0
2	1790.0
3	2628.0
4	3572.0
5	5741.0
6	7606.5
7	8979.5
8	9904.5
9	9789.0
10	8886.5
11	7926.0
12	8443.0
13	10910.0
14	16819.0
15	24799.5
16	31298.0
17	35390.0
18	36726.0
19	35805.5
20	34449.0
21	33912.0
22	35205.0
23	39962.5
24	48459.5
25	60881.0
26	76789.5
27	94944.5
28	115518.0
29	136943.5
30	157098.5
31	177578.0
32	198404.5
33	218874.5
34	238873.0
35	256758.5
36	275550.0
37	292519.5
38	305501.0
39	317658.0
40	329856.0
41	342971.0
42	352635.0
43	359418.0
44	367255.0
45	373755.5
46	377678.0
47	378975.5
48	376744.0
49	370652.5
50	362990.0
51	351816.0
52	335206.5
53	317647.5
54	297996.5
55	274250.0
56	250650.5
57	225451.0
58	198677.0
59	172301.0
60	144767.5
61	118326.0
62	94686.5
63	74425.0
64	56600.0
65	42776.5
66	32910.5
67	25240.0
68	19169.5
69	13964.5
70	10065.5
71	7404.5
72	5351.5
73	3795.5
74	2656.5
75	1819.0
76	1242.5
77	806.0
78	530.0
79	356.5
80	254.5
81	188.0
82	137.5
83	94.0
84	62.5
85	47.0
86	31.0
87	19.0
88	15.5
89	13.5
90	10.5
91	7.0
92	6.0
93	5.5
94	3.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.596572933390293E-4
3	0.004892355631603112
4	6.842455428815542E-5
5	2.280818476271847E-5
6	0.0
7	0.0
8	4.219514181102918E-4
9	0.0027825985410516534
10-11	7.526700971697095E-4
12-13	3.193145866780586E-4
14-15	9.69347852415535E-5
16-17	0.001568062702436895
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	8.003422263359813E-4
32-33	6.990884803054169E-4
34-35	2.8739173378604812E-5
36-37	8.256501200801285E-4
38-39	5.63848553533729E-4
40-41	3.5139995693007865E-5
42-43	8.852825494343251E-5
44-45	0.0010956509208529166
46-47	0.0
48-49	5.470836943832437E-4
50-51	2.4555769252137475E-5
52-53	6.19525715892941E-6
54-55	7.252524785190844E-4
56-57	9.660885857770166E-4
58-59	1.0207440713908404E-4
60-61	1.2248353547306912E-4
62-63	1.3023472791067615E-5
64-65	0.0
66-67	0.0
68-69	3.3608980749851314E-5
70-71	4.827769657454104E-4
72-73	0.0
74-75	1.3904529553451863E-5
76-77	0.0
78-79	3.9824125284422475E-4
80-81	0.0
82-83	0.0
84-85	2.948946945790393E-5
86-87	2.760394751370499E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	2.3669725899840135E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	216.0
20-21	540.0
22-23	1141.0
24-25	2885.0
26-27	4332.0
28-29	8923.0
30-31	19433.0
32-33	24901.0
34-35	34171.0
36-37	54196.0
38-39	64885.0
40-41	64400.0
42-43	71389.0
44-45	84873.0
46-47	86203.0
48-49	82394.0
50-51	75384.0
52-53	72313.0
54-55	77574.0
56-57	81032.0
58-59	81288.0
60-61	78739.0
62-63	76083.0
64-65	78544.0
66-67	83528.0
68-69	84918.0
70-71	83876.0
72-73	79064.0
74-75	80397.0
76-77	79232.0
78-79	84662.0
80-81	84811.0
82-83	80611.0
84-85	79509.0
86-87	82612.0
88-89	88954.0
90-91	95186.0
92-93	93462.0
94-95	104164.0
96-97	160584.0
98-99	730143.0
100-101	5367230.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.20232283132452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.22151090910488	77.77303809716526
2	6.949576158458504	11.98139215104632
3	1.6384261698444738	4.237084248846716
4	0.5702028428555759	1.966112381567014
5	0.25310589978383846	1.0909158241839656
6	0.13808649869228992	0.7142026163352028
7	0.07044959772873484	0.4251043276724543
8	0.04150067838156063	0.2861963900453008
9	0.024300818026399367	0.18853082645192534
>10	0.09139134540081344	1.1869641755987064
>50	0.0011081060674278619	0.0650618376824576
>100	3.0087657760878133E-4	0.052057897568007466
>500	2.0049555531223413E-5	0.013117935582220606
>1k	2.0049522444423915E-5	0.02022129025450697
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1404092381359235E-5
2	0.0	4.561636952543694E-5	0.0	0.0	1.1404092381359235E-5
3	0.0	4.561636952543694E-5	0.0	0.0	1.1404092381359235E-5
4	0.0	6.842455428815541E-5	0.0	3.4212277144077704E-5	1.1404092381359235E-5
5	0.0	7.982864666951464E-5	0.0	3.4212277144077704E-5	1.1404092381359235E-5
6	0.0	7.982864666951464E-5	0.0	3.4212277144077704E-5	1.1404092381359235E-5
7	1.1404092381359235E-5	9.123273905087388E-5	0.0	4.561636952543694E-5	2.280818476271847E-5
8	1.1404092381359235E-5	9.123273905087388E-5	0.0	4.561636952543694E-5	2.280818476271847E-5
9	1.1404092381359235E-5	1.93869570483107E-4	0.0	1.0263683143223312E-4	2.280818476271847E-5
10-11	1.1404092381359235E-5	2.0527366286446624E-4	0.0	1.254450161949516E-4	2.280818476271847E-5
12-13	1.1404092381359235E-5	2.0527366286446624E-4	0.0	1.254450161949516E-4	2.280818476271847E-5
14-15	1.1404092381359235E-5	2.280818476271847E-4	0.0	1.4825320095767004E-4	2.280818476271847E-5
16-17	1.1404092381359235E-5	2.3378389381786433E-4	0.0	1.596572933390293E-4	2.280818476271847E-5
18-19	1.1404092381359235E-5	2.508900323899032E-4	0.0	1.6535933952970891E-4	2.280818476271847E-5
20-21	2.280818476271847E-5	2.7369821715262163E-4	0.0	2.280818476271847E-4	3.4212277144077704E-5
22-23	2.280818476271847E-5	3.193145866780586E-4	0.0	2.6229412477126243E-4	3.4212277144077704E-5
24-25	2.280818476271847E-5	3.535268638221363E-4	0.0	3.2501663286873823E-4	3.4212277144077704E-5
26-27	2.280818476271847E-5	3.535268638221363E-4	0.0	4.276534643009713E-4	3.4212277144077704E-5
28-29	3.4212277144077704E-5	3.649309562034955E-4	0.0	0.0011061969609918458	3.4212277144077704E-5
30-31	3.4212277144077704E-5	4.276534643009713E-4	0.0	0.0030334885734415564	3.4212277144077704E-5
32-33	3.4212277144077704E-5	4.4475960287301017E-4	0.0	0.006882369752150298	3.4212277144077704E-5
34-35	3.4212277144077704E-5	5.188862033518452E-4	0.0	0.01194008472328312	4.561636952543694E-5
36-37	3.4212277144077704E-5	5.816087114493211E-4	0.0	0.018286462133509533	4.561636952543694E-5
38-39	3.4212277144077704E-5	5.987148500213599E-4	0.0	0.02787730382623265	4.561636952543694E-5
40-41	3.4212277144077704E-5	6.27225080974758E-4	0.0	0.03767912122801091	4.561636952543694E-5
42-43	4.561636952543694E-5	6.728414505001949E-4	0.0	0.045935684112115	5.131841571611656E-5
44-45	4.561636952543694E-5	7.241598662163114E-4	0.0	0.05565767286722375	5.702046190679618E-5
46-47	5.131841571611656E-5	7.526700971697095E-4	0.0	0.06570467825520124	5.702046190679618E-5
48-49	5.702046190679618E-5	7.697762357417484E-4	0.0	0.0750845442388692	5.702046190679618E-5
50-51	5.702046190679618E-5	8.667110209833019E-4	0.0	0.08368893194060475	5.702046190679618E-5
52-53	5.702046190679618E-5	8.781151133646611E-4	0.0	0.0917630293466071	5.702046190679618E-5
54-55	5.702046190679618E-5	8.895192057460203E-4	0.0	0.0997344899211772	5.702046190679618E-5
56-57	5.702046190679618E-5	9.009232981273796E-4	0.0	0.10775726891146342	6.27225080974758E-5
58-59	5.702046190679618E-5	9.123273905087388E-4	0.0	0.11544932922269022	6.842455428815541E-5
60-61	5.702046190679618E-5	9.123273905087388E-4	0.0	0.12283347903962033	6.842455428815541E-5
62-63	6.842455428815541E-5	9.123273905087388E-4	0.0	0.12993252654701645	6.842455428815541E-5
64-65	6.842455428815541E-5	9.23731482890098E-4	0.0	0.1367521737910693	6.842455428815541E-5
66-67	7.982864666951464E-5	9.522417138434962E-4	0.0	0.14369156400512637	6.842455428815541E-5
68-69	9.123273905087388E-5	9.69347852415535E-4	0.0	0.15108141586824714	6.842455428815541E-5
70-71	9.123273905087388E-5	9.69347852415535E-4	0.0	0.15770719354181686	8.553069286019427E-5
72-73	9.123273905087388E-5	9.69347852415535E-4	0.0	0.16307852105343706	9.123273905087388E-5
74-75	9.123273905087388E-5	9.807519447968941E-4	0.0	0.16739497001978154	9.123273905087388E-5
76-77	9.123273905087388E-5	9.978580833689332E-4	0.0	0.17140921053802	9.123273905087388E-5
78-79	9.123273905087388E-5	0.0010035601295596127	0.0	0.17493877713005068	9.123273905087388E-5
80-81	9.123273905087388E-5	0.0010035601295596127	0.0	0.17642701118581805	1.0833887762291274E-4
82-83	9.123273905087388E-5	0.0010092621757502923	0.0	0.17690028101964445	1.254450161949516E-4
84-85	9.123273905087388E-5	0.0010491764990850495	0.0	0.1775046979158565	1.254450161949516E-4
86-87	9.123273905087388E-5	0.0011061969609918458	0.0	0.1776472490706235	1.254450161949516E-4
88-89	9.123273905087388E-5	0.0011974297000427198	0.0	0.1777555879482464	1.254450161949516E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8505	0.0	39.383232	1
GTATCAA	14580	0.0	33.677143	1
TCAACGC	16370	0.0	29.038929	4
ATCAACG	16450	0.0	28.949291	3
CAACGCA	16855	0.0	28.228754	5
AACGCAG	17545	0.0	27.11859	6
TATCAAC	17960	0.0	26.797632	2
ACGCAGA	20495	0.0	23.153297	7
CGCAGAG	20965	0.0	22.614067	8
GTACATG	22090	0.0	21.74919	1
TACATGG	21680	0.0	21.61429	2
ACATGGG	21825	0.0	20.734568	3
CATGGGG	15910	0.0	19.245596	4
GCAGAGT	25820	0.0	18.427406	9
AGAGTAC	22490	0.0	17.46065	10-11
GAGTACT	14815	0.0	17.01424	12-13
CAGAGTA	25290	0.0	15.88703	10-11
ATGGGGG	10645	0.0	15.25645	5
GAGTACA	15785	0.0	15.164647	1
AGTACTT	16845	0.0	14.875969	12-13
>>END_MODULE