FastQCFastQC Report
Fri 27 May 2016
SRR2140010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5001657
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47900.043.6912961
GTATCAA80200.033.6861761
TCAACGC92050.027.926044
ATCAACG92900.027.7168713
CAACGCA94850.027.1016565
AACGCAG97450.026.422756
TATCAAC100200.026.0341072
GTACATG128850.024.4125841
TACATGG129600.023.5536082
ACATGGG131150.022.5346853
ACGCAGA115400.022.2395257
CGCAGAG118300.021.6943448
CATGGGG98500.021.2041234
GAGTACT80650.017.92889212-13
GCAGAGT146050.017.630429
ATGGGGG67000.017.1642065
AGAGTAC126900.017.05855610-11
CAGAGTA139800.016.5278710-11
GTGGTAT30100.016.1531261
GAGTACA89750.015.4512861
Produced by FastQC (version 0.11.5)