##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140010_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5001657
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76080946774239	34.0	31.0	34.0	31.0	34.0
2	32.93549377736218	34.0	31.0	34.0	31.0	34.0
3	33.02974474259231	34.0	33.0	34.0	31.0	34.0
4	36.4104701701856	37.0	37.0	37.0	35.0	37.0
5	36.259722328020494	37.0	37.0	37.0	35.0	37.0
6	36.31392516520025	37.0	37.0	37.0	35.0	37.0
7	36.31246384948028	37.0	37.0	37.0	35.0	37.0
8	36.298416504770316	37.0	37.0	37.0	35.0	37.0
9	38.191832026866294	39.0	39.0	39.0	37.0	39.0
10-11	38.091482782605844	39.0	38.0	39.0	37.0	39.0
12-13	38.127829337357596	39.0	38.0	39.0	37.0	39.0
14-15	39.57238671104396	41.0	39.0	41.0	37.0	41.0
16-17	39.552852584653444	41.0	39.0	41.0	37.0	41.0
18-19	39.59811458482659	41.0	39.5	41.0	37.0	41.0
20-21	39.61317815032217	41.0	40.0	41.0	37.0	41.0
22-23	39.551353104509175	41.0	39.0	41.0	37.0	41.0
24-25	39.50780524732167	41.0	39.0	41.0	37.0	41.0
26-27	39.471346982682135	41.0	39.0	41.0	37.0	41.0
28-29	39.37565128320358	40.5	39.0	41.0	36.5	41.0
30-31	39.28471499904502	40.0	39.0	41.0	36.0	41.0
32-33	39.180686169725405	40.0	39.0	41.0	36.0	41.0
34-35	39.1082791562555	40.0	39.0	41.0	35.0	41.0
36-37	39.10958793017328	40.0	39.0	41.0	35.0	41.0
38-39	39.10429199707656	40.0	39.0	41.0	35.0	41.0
40-41	39.0559243466647	40.0	39.0	41.0	35.0	41.0
42-43	39.013881998761484	40.0	39.0	41.0	35.0	41.0
44-45	38.97680367839294	40.0	38.5	41.0	35.0	41.0
46-47	38.89968692259902	40.0	38.0	41.0	35.0	41.0
48-49	38.86519539925044	40.0	38.0	41.0	35.0	41.0
50-51	38.7683799535539	40.0	38.0	41.0	35.0	41.0
52-53	38.59280623562728	40.0	38.0	41.0	35.0	41.0
54-55	38.45298122436466	40.0	38.0	41.0	34.0	41.0
56-57	38.285049562420994	40.0	37.0	41.0	34.0	41.0
58-59	38.08925335501392	40.0	37.0	41.0	34.0	41.0
60-61	37.949775670095526	39.5	36.5	41.0	34.0	41.0
62-63	37.771086453551135	39.0	36.0	41.0	34.0	41.0
64-65	37.514332204148836	39.0	35.5	41.0	33.0	41.0
66-67	37.239767091332	38.5	35.0	40.0	33.0	41.0
68-69	36.98102110893642	38.0	35.0	40.0	33.0	41.0
70-71	36.68096586954907	37.0	35.0	40.0	33.0	41.0
72-73	36.333248505803354	37.0	35.0	39.0	32.0	41.0
74-75	35.956035089982485	36.0	35.0	39.0	32.0	40.5
76-77	34.03651403413294	34.5	32.5	36.5	29.0	39.0
78-79	35.110895721417705	35.0	34.0	37.0	31.0	39.0
80-81	35.040626155977606	35.0	34.0	37.0	32.0	39.0
82-83	34.76579319901788	35.0	34.0	36.5	31.0	39.0
84-85	34.52882181782644	35.0	34.0	36.0	31.0	37.0
86-87	34.32279707302172	35.0	34.0	36.0	31.0	37.0
88-89	34.17133773303337	35.0	34.0	35.5	31.0	37.0
90-91	34.03615980324801	35.0	34.0	35.0	31.0	36.0
92-93	33.95498482729375	35.0	34.0	35.0	31.0	36.0
94-95	33.87730089931794	35.0	34.0	35.0	31.0	36.0
96-97	33.84237629708143	35.0	34.0	35.0	31.0	36.0
98-99	33.871231304045935	35.0	34.0	35.0	31.0	35.0
100-101	32.581415231408634	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	13.0
21	53.0
22	234.0
23	808.0
24	2212.0
25	4800.0
26	9302.0
27	15305.0
28	22685.0
29	32478.0
30	44009.0
31	58148.0
32	78364.0
33	109496.0
34	169899.0
35	280138.0
36	558963.0
37	1209785.0
38	1640939.0
39	747032.0
40	16986.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.724661047328915	19.749335070357684	12.852820575261358	24.673183307052042
2	15.072135238652388	21.263215896683633	40.39931246647354	23.265336398190435
3	18.499690588801016	25.79891571119089	30.10923864850788	25.59215505150022
4	12.416050287354885	17.49754831150889	38.90424269566773	31.18215870546849
5	13.140347916769965	37.89320976892453	35.81887678800781	13.147565526297692
6	30.410002125295676	37.85593454329235	18.70290185832415	13.031161473087819
7	26.841464738585636	32.29585715293952	23.33364722930821	17.529030879166644
8	24.825207122312428	36.52994201494951	21.342145386887566	17.30270547585049
9	25.867485937280694	16.12607122391944	21.028698864065777	36.97774397473409
10-11	24.33045342876922	26.540820585361217	29.143971069330142	19.984754916539416
12-13	25.01055153698556	24.039690173684996	29.212322515750333	21.73743577357911
14-15	22.042814688278746	25.525996631510257	26.523067996788257	25.90812068342274
16-17	21.93650928846113	28.61042766068816	27.253447009366237	22.199616041484475
18-19	21.941848471416574	28.04078728309438	28.621644786917617	21.39571945857143
20-21	22.624798848589684	27.323041992146983	28.462296000704583	21.589863158558746
22-23	22.36038872488289	27.256191148794723	28.30753471386307	22.07588541245932
24-25	22.12077172858989	27.292900369753454	28.46120648537435	22.12512141628231
26-27	22.120777210089795	27.500166785642094	28.29821732904322	22.080838675224896
28-29	22.072683234049332	27.62770336356639	28.335036188295028	21.96457721408925
30-31	22.341208824192318	27.47765385047474	28.211483251574588	21.969654073758356
32-33	22.08582634129211	27.63497250479633	28.199170889750075	22.080030264161486
34-35	22.211140415934143	27.62990523997708	28.21704842140347	21.941905922685308
36-37	22.218062633687506	27.56572478806087	28.171597702004448	22.04461487624718
38-39	22.04844231289818	27.706919248989593	28.284009826902373	21.960628611209852
40-41	22.29734653140186	27.548330485383087	28.117622910861723	22.03670007235333
42-43	22.159371245716166	27.629212515229135	28.025467365516942	22.185948873537754
44-45	22.211130979829193	27.74281857124674	27.913019528144606	22.133030920779458
46-47	22.290556233134023	27.695067767449448	27.671806894493233	22.342569104923296
48-49	22.199440395426357	27.78063304801571	27.737511191718816	22.28241536483912
50-51	22.20334798950536	27.91743802056308	27.651695896221657	22.227518093709904
52-53	22.405700445420525	27.785733394692834	27.458399536722727	22.350166623163904
54-55	22.313415913273552	27.684165436547755	27.61325849307369	22.38916015710501
56-57	22.313178535743784	27.78160158630482	27.58017587407844	22.32504400387296
58-59	22.283668150487106	27.83677186835312	27.54159564952267	22.337964331637103
60-61	22.342653966004534	27.810492526466945	27.54503710369732	22.301816403831204
62-63	22.31326963896915	27.83496682180482	27.552585640903292	22.299177898322743
64-65	22.27238872927341	27.804901488453137	27.52111125940349	22.40159852286996
66-67	22.330353561327268	27.88210200260149	27.385990932084965	22.40155350398628
68-69	22.269384135842287	27.901057800827346	27.42945693887292	22.400101124457446
70-71	22.289238880763836	27.659893554228056	27.476256950543842	22.57461061446426
72-73	22.30533416409218	27.699028762140472	27.45695246117116	22.53868461259619
74-75	22.167423697192	27.81540079684141	27.446373477276264	22.570802028690327
76-77	22.352634856474698	27.760176625352834	27.32105845455765	22.566130063614814
78-79	22.265220755866576	27.78508069237265	27.328017397067228	22.621681154693547
80-81	22.30947221581994	27.83732181660257	27.314698324927612	22.538507642649883
82-83	22.366311961807313	27.777715514595435	27.380681596755156	22.47529092684209
84-85	22.440556655940597	27.749097605513466	27.320445726819	22.48990001172694
86-87	22.29735994440588	27.81908490608973	27.37029723266945	22.51325791683494
88-89	22.313457295884504	27.759913434159543	27.4121551899227	22.514474080033256
90-91	22.295548144857282	27.76730264597277	27.474686288324722	22.462462920845226
92-93	22.357593318283612	27.686261320398852	27.480564930877836	22.475580430439695
94-95	22.21018168753241	27.759852546648915	27.558702087346177	22.471263678472503
96-97	22.229787948121352	27.76923691040947	27.580915344952434	22.42005979651674
98-99	22.461160846779222	28.403782811382268	27.6198462695089	21.515210072329605
100-101	23.286453203065673	30.940540127480006	24.64811081534602	21.1248958541083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	855.0
1	767.0
2	1016.5
3	1447.0
4	2030.5
5	3407.0
6	4538.5
7	5192.0
8	5568.5
9	5406.0
10	4863.5
11	4402.0
12	4809.0
13	6399.0
14	9910.0
15	14434.5
16	18084.5
17	20274.0
18	20905.5
19	20334.0
20	19462.0
21	18904.0
22	19295.5
23	21697.0
24	26483.5
25	33211.5
26	41899.5
27	52123.5
28	63657.0
29	75734.0
30	86772.0
31	98062.5
32	109906.0
33	120604.5
34	130946.5
35	140560.5
36	151066.0
37	161559.5
38	169226.0
39	175103.5
40	181394.5
41	188388.0
42	193399.0
43	198364.5
44	203425.0
45	207864.5
46	210946.0
47	212559.5
48	212456.5
49	209848.5
50	206607.0
51	201249.0
52	193073.0
53	184137.5
54	174752.5
55	163455.0
56	151445.5
57	137489.5
58	122697.0
59	107737.0
60	91224.5
61	75229.0
62	60676.5
63	48036.0
64	36506.0
65	27391.5
66	21028.0
67	16207.5
68	12280.5
69	9037.0
70	6720.0
71	4963.5
72	3539.0
73	2512.0
74	1801.5
75	1275.0
76	872.0
77	583.5
78	404.0
79	273.5
80	175.5
81	112.5
82	78.5
83	55.5
84	40.5
85	33.5
86	27.0
87	18.5
88	13.0
89	11.0
90	7.5
91	5.0
92	4.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.9993374195791515E-4
3	0.004438529071465716
4	3.998674839158303E-5
5	1.9993374195791516E-5
6	0.0
7	0.0
8	4.5984760650320485E-4
9	0.0026991055164318546
10-11	8.897051517127223E-4
12-13	3.1989398713266426E-4
14-15	1.7994036776212364E-4
16-17	0.0014995030646843635
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	7.416007452085326E-4
32-33	8.739242695399422E-4
34-35	3.022615206978614E-5
36-37	8.197662026789961E-4
38-39	7.131819560482262E-4
40-41	4.105151821856116E-5
42-43	8.273184695228802E-5
44-45	0.001158029142898814
46-47	0.0
48-49	4.4718762108004117E-4
50-51	0.0
52-53	7.593992197498474E-5
54-55	8.755218109993557E-4
56-57	0.0010605892545515851
58-59	1.2271568927896612E-4
60-61	1.4649214418973843E-4
62-63	1.1382664816148905E-5
64-65	6.897315185577438E-5
66-67	1.1616892541652949E-5
68-69	1.1747728341770551E-5
70-71	5.109903325382483E-4
72-73	0.0
74-75	1.2151046253686627E-5
76-77	0.0
78-79	3.107319739099491E-4
80-81	2.5161809305189347E-5
82-83	3.820693820167838E-5
84-85	1.2886745737161162E-5
86-87	3.5209137057955805E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	2.7573735961349343E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	89.0
20-21	284.0
22-23	618.0
24-25	1292.0
26-27	2292.0
28-29	5297.0
30-31	11030.0
32-33	13944.0
34-35	19342.0
36-37	30693.0
38-39	35928.0
40-41	36408.0
42-43	40103.0
44-45	47891.0
46-47	48770.0
48-49	46389.0
50-51	42634.0
52-53	39445.0
54-55	42567.0
56-57	43718.0
58-59	44231.0
60-61	45472.0
62-63	42800.0
64-65	44613.0
66-67	47514.0
68-69	48595.0
70-71	48281.0
72-73	45273.0
74-75	45862.0
76-77	45497.0
78-79	48295.0
80-81	48857.0
82-83	46486.0
84-85	45297.0
86-87	47156.0
88-89	50573.0
90-91	53995.0
92-93	53443.0
94-95	59155.0
96-97	90994.0
98-99	417370.0
100-101	3073164.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.73229989189552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.68289059075634	81.35233742854838
2	6.228723484021996	11.053779202558642
3	1.3006863677430596	3.4623867854363235
4	0.3971706960188314	1.409674772296633
5	0.16178546707952685	0.7177798291525482
6	0.07873411772865463	0.4191755607613674
7	0.0470570933098236	0.2922838880925724
8	0.025874995193123078	0.18367582665420415
9	0.01956662957401393	0.15625728389115323
>10	0.05599108852686887	0.7505937076892187
>50	8.596220299267073E-4	0.04917426558160591
>100	5.665403732621943E-4	0.10433655758026408
>500	9.330764452769847E-5	0.048544891757115155
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9993374195791516E-5
2	0.0	0.0	0.0	0.0	1.9993374195791516E-5
3	0.0	0.0	0.0	0.0	1.9993374195791516E-5
4	0.0	0.0	0.0	1.9993374195791516E-5	1.9993374195791516E-5
5	1.9993374195791516E-5	0.0	0.0	1.9993374195791516E-5	1.9993374195791516E-5
6	1.399536193705406E-4	0.0	0.0	1.9993374195791516E-5	1.9993374195791516E-5
7	1.399536193705406E-4	0.0	0.0	1.9993374195791516E-5	1.9993374195791516E-5
8	1.399536193705406E-4	0.0	0.0	1.9993374195791516E-5	1.9993374195791516E-5
9	1.399536193705406E-4	1.1996024517474908E-4	0.0	3.998674839158303E-5	1.9993374195791516E-5
10-11	1.6994368066422787E-4	1.1996024517474908E-4	0.0	8.997018388106182E-5	1.9993374195791516E-5
12-13	1.7994036776212364E-4	1.2995693227264485E-4	0.0	9.996687097895757E-5	1.9993374195791516E-5
14-15	1.9993374195791515E-4	2.1992711615370666E-4	0.0	1.399536193705406E-4	1.9993374195791516E-5
16-17	2.3992049034949817E-4	2.3992049034949817E-4	0.0	1.7994036776212364E-4	1.9993374195791516E-5
18-19	2.3992049034949817E-4	2.6991055164318545E-4	0.0	1.7994036776212364E-4	3.998674839158303E-5
20-21	2.3992049034949817E-4	2.999006129368727E-4	0.0	2.2992380325160243E-4	3.998674839158303E-5
22-23	2.499171774473939E-4	3.69877422622143E-4	0.0	3.3988736132845574E-4	3.998674839158303E-5
24-25	3.0989730003476846E-4	3.898707968179345E-4	0.0	3.998674839158303E-4	3.998674839158303E-5
26-27	3.1989398713266426E-4	3.998674839158303E-4	0.0	4.7984098069899634E-4	3.998674839158303E-5
28-29	3.1989398713266426E-4	3.998674839158303E-4	0.0	0.0010596488323769502	3.998674839158303E-5
30-31	3.1989398713266426E-4	4.398542323074133E-4	0.0	0.0029390260067813524	3.998674839158303E-5
32-33	3.3988736132845574E-4	4.398542323074133E-4	0.0	0.006377886368457493	3.998674839158303E-5
34-35	3.4988404842635154E-4	4.7984098069899634E-4	0.0	0.010646471759258981	3.998674839158303E-5
36-37	3.598807355242473E-4	5.198277290905794E-4	0.0	0.017214295182576493	3.998674839158303E-5
38-39	3.69877422622143E-4	5.498177903842667E-4	0.0	0.03004004472917675	3.998674839158303E-5
40-41	3.998674839158303E-4	6.5978134846112E-4	0.0	0.06038998675838827	3.998674839158303E-5
42-43	3.998674839158303E-4	6.797747226569115E-4	0.0	0.0730457926243243	3.998674839158303E-5
44-45	3.998674839158303E-4	7.197614710484944E-4	0.0	0.0895403263358523	3.998674839158303E-5
46-47	3.998674839158303E-4	7.39754845244286E-4	0.0	0.10733442937010675	3.998674839158303E-5
48-49	3.998674839158303E-4	7.39754845244286E-4	0.0	0.12431880074943163	3.998674839158303E-5
50-51	4.1986085811162183E-4	9.396885872022012E-4	0.0	0.14169304292557444	3.998674839158303E-5
52-53	4.1986085811162183E-4	9.396885872022012E-4	0.0	0.16487536030559474	5.998012258737455E-5
54-55	4.1986085811162183E-4	9.596819613979927E-4	0.0	0.21085012426881733	7.997349678316607E-5
56-57	4.1986085811162183E-4	9.796753355937843E-4	0.0	0.25853432172578006	7.997349678316607E-5
58-59	4.1986085811162183E-4	0.0010196620839853672	0.0	0.3070082574634766	7.997349678316607E-5
60-61	4.398542323074133E-4	0.0010196620839853672	0.0	0.34132688427055274	7.997349678316607E-5
62-63	4.5984760650320485E-4	0.001029658771083263	0.0	0.3558420739366974	7.997349678316607E-5
64-65	4.5984760650320485E-4	0.0012595825743348654	0.0	0.36984743256084934	7.997349678316607E-5
66-67	4.7984098069899634E-4	0.0012695792614327611	0.0	0.3772149909519985	7.997349678316607E-5
68-69	4.7984098069899634E-4	0.0013295593840201357	0.0	0.38397275143017606	7.997349678316607E-5
70-71	5.198277290905794E-4	0.001349552758215927	0.0	0.3906505384115704	7.997349678316607E-5
72-73	5.598144774821624E-4	0.001359549445313823	0.0	0.39612872294121726	7.997349678316607E-5
74-75	5.598144774821624E-4	0.0013895395066075102	0.0	0.4009571228095009	7.997349678316607E-5
76-77	5.698111645800582E-4	0.0014695130033906762	0.0	0.4050057810841487	7.997349678316607E-5
78-79	5.79807851677954E-4	0.001519496438880155	0.0	0.4085845950651954	7.997349678316607E-5
80-81	5.79807851677954E-4	0.001519496438880155	0.0	0.41011408819117345	9.996687097895757E-5
82-83	5.79807851677954E-4	0.0015394898130759466	0.0	0.41058393248477454	1.399536193705406E-4
84-85	5.79807851677954E-4	0.0015794765614675298	0.0	0.4109438132202988	1.399536193705406E-4
86-87	5.79807851677954E-4	0.0016194633098591128	0.0	0.41113375027515886	1.399536193705406E-4
88-89	5.79807851677954E-4	0.0016694467453485917	0.0	0.41124371383323566	1.5994699356633213E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4790	0.0	43.691296	1
GTATCAA	8020	0.0	33.686176	1
TCAACGC	9205	0.0	27.92604	4
ATCAACG	9290	0.0	27.716871	3
CAACGCA	9485	0.0	27.101656	5
AACGCAG	9745	0.0	26.42275	6
TATCAAC	10020	0.0	26.034107	2
GTACATG	12885	0.0	24.412584	1
TACATGG	12960	0.0	23.553608	2
ACATGGG	13115	0.0	22.534685	3
ACGCAGA	11540	0.0	22.239525	7
CGCAGAG	11830	0.0	21.694344	8
CATGGGG	9850	0.0	21.204123	4
GAGTACT	8065	0.0	17.928892	12-13
GCAGAGT	14605	0.0	17.63042	9
ATGGGGG	6700	0.0	17.164206	5
AGAGTAC	12690	0.0	17.058556	10-11
CAGAGTA	13980	0.0	16.52787	10-11
GTGGTAT	3010	0.0	16.153126	1
GAGTACA	8975	0.0	15.451286	1
>>END_MODULE