FastQCFastQC Report
Fri 27 May 2016
SRR2140009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4910667
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53450.044.2588651
GTATCAA95100.036.8277171
ATCAACG116500.029.6322673
TCAACGC117900.029.2095434
CAACGCA117950.029.0904715
TATCAAC123250.028.2121682
AACGCAG122450.028.1587186
GTACATG162900.025.4907741
TACATGG166450.024.6447222
ACATGGG166700.023.9044653
ACGCAGA146650.023.4547947
CGCAGAG148350.023.1860168
CATGGGG127750.021.50684
ACCGAAC6850.020.2079948
GCAGAGT178350.019.2153729
AGAGTAC154000.018.34393110-11
GAGTACA111600.018.1531431
GAGTACT93650.017.8704812-13
CCGAACC6850.017.7585419
CAGAGTA172000.016.64369610-11
Produced by FastQC (version 0.11.5)