##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140009_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4910667
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.746865547999896	34.0	31.0	34.0	31.0	34.0
2	32.92518613051954	34.0	31.0	34.0	31.0	34.0
3	33.019706284299055	34.0	33.0	34.0	31.0	34.0
4	36.40674922571618	37.0	37.0	37.0	35.0	37.0
5	36.25098952952827	37.0	37.0	37.0	35.0	37.0
6	36.30635553174345	37.0	37.0	37.0	35.0	37.0
7	36.302544033224	37.0	37.0	37.0	35.0	37.0
8	36.29076029793916	37.0	37.0	37.0	35.0	37.0
9	38.181346444383216	39.0	39.0	39.0	37.0	39.0
10-11	38.082063902928056	39.0	38.0	39.0	37.0	39.0
12-13	38.11299137164055	39.0	38.0	39.0	37.0	39.0
14-15	39.539578126555924	41.0	39.0	41.0	37.0	41.0
16-17	39.52084299342635	41.0	39.0	41.0	37.0	41.0
18-19	39.56818360927345	41.0	39.5	41.0	37.0	41.0
20-21	39.581608134826865	41.0	39.5	41.0	37.0	41.0
22-23	39.51599038585188	41.0	39.0	41.0	37.0	41.0
24-25	39.46563005074199	41.0	39.0	41.0	37.0	41.0
26-27	39.41983790093059	41.0	39.0	41.0	36.5	41.0
28-29	39.32516975607503	40.0	39.0	41.0	36.0	41.0
30-31	39.223106191395644	40.0	39.0	41.0	36.0	41.0
32-33	39.114723645284975	40.0	39.0	41.0	35.5	41.0
34-35	39.03815485758495	40.0	39.0	41.0	35.0	41.0
36-37	39.035172829916725	40.0	39.0	41.0	35.0	41.0
38-39	39.0216622132749	40.0	39.0	41.0	35.0	41.0
40-41	38.96915819790496	40.0	38.5	41.0	35.0	41.0
42-43	38.92281768794251	40.0	38.0	41.0	35.0	41.0
44-45	38.87668252994116	40.0	38.0	41.0	35.0	41.0
46-47	38.78988715336364	40.0	38.0	41.0	35.0	41.0
48-49	38.74669781331028	40.0	38.0	41.0	35.0	41.0
50-51	38.632843893143686	40.0	38.0	41.0	35.0	41.0
52-53	38.44388267252515	40.0	38.0	41.0	34.5	41.0
54-55	38.28308345661693	40.0	37.0	41.0	34.0	41.0
56-57	38.09703953141306	40.0	37.0	41.0	34.0	41.0
58-59	37.880333064277664	39.0	36.0	41.0	34.0	41.0
60-61	37.71741509319956	39.0	36.0	41.0	33.0	41.0
62-63	37.5170692684174	39.0	35.5	41.0	33.0	41.0
64-65	37.2395262845569	38.5	35.0	40.0	33.0	41.0
66-67	36.95075348133143	38.0	35.0	40.0	33.0	41.0
68-69	36.68443131397186	37.0	35.0	40.0	33.0	41.0
70-71	36.38442690062014	37.0	35.0	39.0	32.5	41.0
72-73	36.03948136239649	36.0	35.0	39.0	32.0	41.0
74-75	35.671140606684716	36.0	35.0	39.0	32.0	40.0
76-77	33.79037755518729	34.5	32.0	36.5	29.0	39.0
78-79	34.87563685008996	35.0	34.0	37.0	31.0	39.0
80-81	34.82890459120382	35.0	34.0	36.5	31.5	39.0
82-83	34.59146332339333	35.0	34.0	36.0	31.0	38.0
84-85	34.380468871677316	35.0	34.0	36.0	31.0	37.0
86-87	34.20388817524063	35.0	34.0	35.5	31.0	37.0
88-89	34.077385845746015	35.0	34.0	35.0	31.0	36.5
90-91	33.95695654768865	35.0	34.0	35.0	31.0	36.0
92-93	33.88720142046212	35.0	34.0	35.0	31.0	36.0
94-95	33.824607054697935	35.0	34.0	35.0	31.0	36.0
96-97	33.79057587712691	35.0	34.0	35.0	31.0	35.5
98-99	33.82292066362119	35.0	34.0	35.0	31.0	35.0
100-101	32.56183901397219	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	11.0
21	61.0
22	265.0
23	845.0
24	2346.0
25	4985.0
26	9369.0
27	15385.0
28	23215.0
29	32504.0
30	44180.0
31	59179.0
32	79463.0
33	113388.0
34	180080.0
35	298075.0
36	596036.0
37	1246846.0
38	1518903.0
39	670900.0
40	14623.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.285504800060764	19.473994062313736	12.74863475776305	24.49186637986245
2	15.388385833425989	21.08047434783689	39.90619994306262	23.624939875674503
3	18.615337933056917	26.083691074526925	29.8930238430772	25.40794714933896
4	12.817332405013335	17.24830231681547	37.844482506740945	32.08988277143025
5	13.346644497232043	37.9640028387194	35.24775157743401	13.44160108661454
6	30.927407621001386	37.0576746498999	18.413242030054167	13.601675699044549
7	27.166004129377946	32.203649728234474	22.97417031128358	17.656175831104004
8	25.588985884539788	35.81906975948572	21.004088262150546	17.587856093823948
9	26.402765810866484	15.861095266479328	20.602802426051277	37.133336496602915
10-11	25.03580249465368	26.112295561773596	28.485712042762817	20.366189900809907
12-13	25.62947331037237	23.728300492826516	28.70915538116825	21.93307081563287
14-15	22.689536640695955	24.976041916972463	26.064083035681556	26.270338406650023
16-17	22.44831588807885	28.137457744471146	26.668645379383378	22.74558098806663
18-19	22.464942135151905	27.418301831502728	28.292317520206524	21.824438513138848
20-21	23.198811287030438	26.610781218530576	28.063356210064068	22.127051284374915
22-23	23.057953809736485	26.622230314272276	27.902980528776094	22.416835347215137
24-25	22.739027062880808	26.820103865954124	28.00082382206584	22.440045249099224
26-27	22.714529743123656	26.868813907899764	27.98063800111747	22.436018347859115
28-29	22.590995495106434	26.9598866949467	27.96050394194758	22.488613867999284
30-31	22.891457855303425	26.830972436675353	27.83760563469469	22.43996407332653
32-33	22.602970899877796	27.03200011960441	27.854915341989773	22.510113638528022
34-35	22.700446371838044	27.023048418949493	27.92261367667532	22.353891532537144
36-37	22.75110510431588	26.993861294298753	27.7730059012875	22.482027700097866
38-39	22.621707871583666	27.000913493221155	27.900767211633415	22.476611423561764
40-41	22.749739397582594	26.853196329921353	27.828061626379203	22.569002646116854
42-43	22.66323138645729	27.022635370907423	27.654907592730954	22.659225649904332
44-45	22.70412799125838	27.1256228481427	27.519593139240005	22.65065602135891
46-47	22.89549755439093	27.109778875505384	27.19615478822525	22.79856878187843
48-49	22.815246291364502	27.245850594112657	27.137032677014588	22.80187043750825
50-51	22.737805874546858	27.31684579165629	27.124517359402635	22.820830974394216
52-53	22.91996147186904	27.170718175007654	27.028871486365535	22.88044886675777
54-55	22.79297535604027	27.148790290661275	27.082966018753734	22.975268334544722
56-57	22.887042820153447	27.272601242562388	26.98039204100088	22.85996389628329
58-59	22.801960874879445	27.39794808089011	27.012492270317264	22.787598773913185
60-61	22.88464117153151	27.38441423773134	26.967846048481796	22.763098542255353
62-63	22.87431492903084	27.4524442138453	26.885647341690827	22.787593515433027
64-65	22.898122828563206	27.447555001813228	26.808440328569443	22.845881841054123
66-67	22.9585004441334	27.324226035061542	26.79235946350223	22.92491405730282
68-69	22.82913642203562	27.307563391498054	26.9316024185651	22.93169776790123
70-71	22.848138419688695	27.1766709983135	26.907527895054745	23.06766268694306
72-73	22.92571529077754	27.228110281063945	26.864118295090982	22.982056133067527
74-75	22.711721018950815	27.38309039208667	26.944245043742782	22.96094354521973
76-77	22.9700351713752	27.254890037343692	26.821931160787965	22.953143630493145
78-79	22.86646566591187	27.308295067800664	26.78356775608663	23.041671510200835
80-81	22.868685596293435	27.35352715953283	26.843008200526842	22.934779043646895
82-83	22.930222960005494	27.293814511454563	26.86055141637137	22.915411112168574
84-85	22.931511203434933	27.30025720182531	26.817745827136896	22.95048576760286
86-87	22.827471259455532	27.31246824413199	26.96212088835705	22.897939608055427
88-89	22.8642502499822	27.266067662489803	26.90209853078926	22.96758355673874
90-91	22.9230080872639	27.264124259113792	26.875940055164783	22.93692759845753
92-93	22.913845114765333	27.276789728217736	26.906167897706958	22.90319725930997
94-95	22.78146287639181	27.242326151381036	26.97887111609434	22.997339856132818
96-97	22.909373750360803	27.28041039980843	26.88253113449528	22.927684715335484
98-99	23.0827363912116	27.987816351920902	26.90765711863757	22.02179013822993
100-101	27.45065713160303	34.82381883642344	27.52665200999444	10.198872021979085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1200.0
1	1077.0
2	1551.0
3	2287.0
4	2941.5
5	4637.0
6	6210.5
7	7421.0
8	8205.5
9	7986.0
10	7004.0
11	5929.0
12	6067.0
13	7614.5
14	11646.0
15	17141.5
16	21451.5
17	23746.0
18	24018.5
19	22921.5
20	21041.0
21	19164.5
22	18567.5
23	20206.5
24	24105.0
25	30172.0
26	37499.0
27	45002.0
28	54564.0
29	65694.0
30	75901.0
31	85914.0
32	96468.0
33	106322.0
34	114212.0
35	121779.0
36	130624.0
37	139447.0
38	147805.0
39	153895.0
40	159050.0
41	165972.0
42	171926.0
43	178033.0
44	186013.0
45	194467.0
46	202463.0
47	209173.0
48	212254.5
49	211570.0
50	211074.5
51	210768.0
52	206520.0
53	200729.0
54	195071.5
55	185537.5
56	173988.5
57	160078.0
58	143574.0
59	126497.0
60	108447.0
61	90879.0
62	74194.5
63	58962.5
64	45311.0
65	34579.5
66	27030.0
67	21283.5
68	16525.5
69	11894.5
70	8400.5
71	6190.5
72	4575.5
73	3304.5
74	2297.0
75	1598.0
76	1116.5
77	740.5
78	499.0
79	325.0
80	205.5
81	135.0
82	97.5
83	74.0
84	50.5
85	34.0
86	27.5
87	23.5
88	15.5
89	8.5
90	5.5
91	4.0
92	2.5
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.8327449203947243E-4
3	0.004622589965884471
4	8.145532979532108E-5
5	4.072766489766054E-5
6	0.0
7	0.0
8	4.683681463230962E-4
9	0.002769481213040917
10-11	9.265543764217772E-4
12-13	2.850936542836238E-4
14-15	1.425468271418119E-4
16-17	0.0013643767740716282
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	7.047199795161392E-4
32-33	6.656023173610281E-4
34-35	1.8492205175947682E-4
36-37	8.980520940407122E-4
38-39	5.509829744181723E-4
40-41	5.240870403756656E-5
42-43	1.5853813465721838E-4
44-45	0.0011631165291236909
46-47	0.0
48-49	5.891450675902134E-4
50-51	3.308211145473623E-5
52-53	1.1136584191351462E-5
54-55	7.983406209088557E-4
56-57	9.431512729417287E-4
58-59	1.2638632876783786E-4
60-61	2.207748430087521E-4
62-63	1.1749275627784359E-5
64-65	0.0
66-67	0.0
68-69	2.4323804320831954E-5
70-71	5.416067873177847E-4
72-73	0.0
74-75	1.2600358707011671E-5
76-77	0.0
78-79	3.7414951943719654E-4
80-81	0.0
82-83	0.0
84-85	1.3400114142172263E-5
86-87	3.934489928655487E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.4378376761782505E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	103.0
20-21	269.0
22-23	685.0
24-25	2386.0
26-27	2725.0
28-29	6204.0
30-31	12076.0
32-33	14882.0
34-35	20271.0
36-37	31486.0
38-39	39725.0
40-41	39018.0
42-43	42819.0
44-45	50996.0
46-47	51549.0
48-49	49731.0
50-51	45467.0
52-53	42390.0
54-55	45761.0
56-57	48213.0
58-59	48615.0
60-61	48135.0
62-63	46133.0
64-65	47457.0
66-67	50030.0
68-69	49250.0
70-71	48788.0
72-73	46098.0
74-75	45957.0
76-77	45826.0
78-79	48764.0
80-81	49661.0
82-83	46583.0
84-85	45713.0
86-87	47148.0
88-89	50869.0
90-91	54510.0
92-93	53041.0
94-95	57874.0
96-97	89025.0
98-99	400620.0
100-101	2943814.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.53461258434326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.63924405023863	76.67258012190558
2	7.377629156711716	12.620853034205837
3	1.7793771974568668	4.565950176776627
4	0.600946156406968	2.056067866892807
5	0.256587253616603	1.0973545666088387
6	0.12021232027850867	0.6169388545732339
7	0.06524669465112522	0.39065955245750567
8	0.04394266359364527	0.30068949651252586
9	0.02756976953125682	0.2122352600306115
>10	0.08655076299132215	1.126845065330226
>50	0.0016766700964018174	0.09211564379817859
>100	9.425857458332854E-4	0.18750682590924544
>500	3.735934054216449E-5	0.02639496810456166
>1k	3.7359340542104436E-5	0.033808566894166274
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.072766489766054E-5	0.0	0.0	0.0
3	0.0	4.072766489766054E-5	0.0	0.0	0.0
4	0.0	6.109149734649082E-5	0.0	0.0	0.0
5	0.0	8.145532979532108E-5	0.0	0.0	0.0
6	0.0	8.145532979532108E-5	0.0	0.0	0.0
7	0.0	8.145532979532108E-5	0.0	0.0	0.0
8	0.0	1.0181916224415135E-4	0.0	0.0	0.0
9	0.0	2.2400215693713297E-4	0.0	0.0	6.109149734649082E-5
10-11	4.072766489766054E-5	2.2400215693713297E-4	0.0	0.0	6.109149734649082E-5
12-13	4.072766489766054E-5	2.2400215693713297E-4	0.0	0.0	6.109149734649082E-5
14-15	4.072766489766054E-5	3.6654898407894487E-4	0.0	0.0	7.127341357090595E-5
16-17	4.072766489766054E-5	3.8691281652777513E-4	0.0	0.0	1.2218299469298163E-4
18-19	4.072766489766054E-5	4.1745856520102053E-4	0.0	0.0	1.2218299469298163E-4
20-21	4.072766489766054E-5	4.4800431387426594E-4	0.0	0.0	1.2218299469298163E-4
22-23	4.072766489766054E-5	5.803692247916628E-4	0.0	4.072766489766054E-5	1.3236491091739676E-4
24-25	4.072766489766054E-5	7.229160519334745E-4	0.0	4.072766489766054E-5	1.425468271418119E-4
26-27	4.072766489766054E-5	7.738256330555503E-4	0.0	5.090958112207568E-5	1.425468271418119E-4
28-29	4.072766489766054E-5	7.738256330555503E-4	0.0	2.1382024071271786E-4	1.5272874336622703E-4
30-31	4.072766489766054E-5	8.756447952997016E-4	0.0	0.0013032852767251373	1.6291065959064217E-4
32-33	4.072766489766054E-5	8.960086277485319E-4	0.0	0.0028611184590606534	1.6291065959064217E-4
34-35	4.072766489766054E-5	0.0010283735386659287	0.0	0.004907683620168095	1.6291065959064217E-4
36-37	4.072766489766054E-5	0.0011709203658077406	0.0	0.008074259565961202	1.6291065959064217E-4
38-39	4.072766489766054E-5	0.0012320118631542316	0.0	0.013715041154287188	1.9345640826388757E-4
40-41	8.145532979532108E-5	0.0013236491091739677	0.0	0.0195085514859794	2.036383244883027E-4
42-43	8.145532979532108E-5	0.0013847406065204584	0.0	0.025291879901447198	2.1382024071271783E-4
44-45	9.163724601973622E-5	0.0014763778525401948	0.0	0.030790114662631367	2.2400215693713297E-4
46-47	1.0181916224415135E-4	0.0015272874336622704	0.0	0.03719453996778849	2.3418407316154813E-4
48-49	1.0181916224415135E-4	0.0015272874336622704	0.0	0.044301517492430255	2.4436598938596326E-4
50-51	1.0181916224415135E-4	0.001639288512130837	0.0	0.05174449825247772	2.4436598938596326E-4
52-53	1.0181916224415135E-4	0.001649470428355252	0.0	0.057711101159984984	2.545479056103784E-4
54-55	1.0181916224415135E-4	0.0016800161770284972	0.0	0.06392207005687822	2.7491173805920866E-4
56-57	1.0181916224415135E-4	0.0017105619257017427	0.0	0.07033667727825976	2.850936542836238E-4
58-59	1.0181916224415135E-4	0.001720743841926158	0.0	0.07625237060464496	2.850936542836238E-4
60-61	1.0181916224415135E-4	0.0017512895905994032	0.0	0.0830131629776566	2.850936542836238E-4
62-63	1.0181916224415135E-4	0.0017614715068238183	0.0	0.08918340420965218	2.850936542836238E-4
64-65	1.0181916224415135E-4	0.0019142002501900456	0.0	0.0951296432847106	2.850936542836238E-4
66-67	1.0181916224415135E-4	0.0019345640826388757	0.0	0.10141188559517475	2.850936542836238E-4
68-69	1.0181916224415135E-4	0.001965109831312121	0.0	0.10809122263839108	2.850936542836238E-4
70-71	1.0181916224415135E-4	0.0019854736637609513	0.0	0.11424110003793782	3.2582131918128433E-4
72-73	1.0181916224415135E-4	0.0019956555799853664	0.0	0.11930151240147215	3.8691281652777513E-4
74-75	1.0181916224415135E-4	0.0020567470773318573	0.0	0.12314009481807664	3.8691281652777513E-4
76-77	1.0181916224415135E-4	0.002138202407127179	0.0	0.12733504430253567	3.8691281652777513E-4
78-79	1.0181916224415135E-4	0.002178930072024839	0.0	0.1305016202483288	3.8691281652777513E-4
80-81	1.0181916224415135E-4	0.002178930072024839	0.0	0.13173363211148303	4.072766489766054E-4
82-83	1.120010784685665E-4	0.002178930072024839	0.0	0.13222236409025495	4.2764048142543567E-4
84-85	1.2218299469298163E-4	0.002178930072024839	0.0	0.13261945882300713	4.2764048142543567E-4
86-87	1.2218299469298163E-4	0.0021992939044736693	0.0	0.13287400672861752	4.2764048142543567E-4
88-89	1.2218299469298163E-4	0.0023418407316154813	0.0	0.13297582589086168	4.2764048142543567E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5345	0.0	44.258865	1
GTATCAA	9510	0.0	36.827717	1
ATCAACG	11650	0.0	29.632267	3
TCAACGC	11790	0.0	29.209543	4
CAACGCA	11795	0.0	29.090471	5
TATCAAC	12325	0.0	28.212168	2
AACGCAG	12245	0.0	28.158718	6
GTACATG	16290	0.0	25.490774	1
TACATGG	16645	0.0	24.644722	2
ACATGGG	16670	0.0	23.904465	3
ACGCAGA	14665	0.0	23.454794	7
CGCAGAG	14835	0.0	23.186016	8
CATGGGG	12775	0.0	21.5068	4
ACCGAAC	685	0.0	20.207994	8
GCAGAGT	17835	0.0	19.215372	9
AGAGTAC	15400	0.0	18.343931	10-11
GAGTACA	11160	0.0	18.153143	1
GAGTACT	9365	0.0	17.87048	12-13
CCGAACC	685	0.0	17.758541	9
CAGAGTA	17200	0.0	16.643696	10-11
>>END_MODULE