FastQCFastQC Report
Fri 27 May 2016
SRR2140008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9833442
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105600.045.774991
GTATCAA180250.035.0401731
TCAACGC215100.028.2659634
ATCAACG216500.028.045573
CAACGCA219800.027.6423935
TATCAAC225250.027.5165042
AACGCAG227850.026.7766596
GTACATG294200.024.917611
TACATGG299500.024.265782
ACATGGG307150.023.2779143
ACGCAGA271500.022.394147
CGCAGAG277700.021.8941638
CATGGGG242200.021.1048184
GAGTACT177450.018.37714412-13
GCAGAGT336250.018.2070319
ATGGGGG163300.017.197955
CAGAGTA315800.017.07923910-11
AGAGTAC287650.017.0234210-11
GAGTACA206000.016.9448451
GTACTTT200650.016.20045914-15
Produced by FastQC (version 0.11.5)