##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140008_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9833442 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.755855172583516 34.0 31.0 34.0 31.0 34.0 2 32.933433481379154 34.0 31.0 34.0 31.0 34.0 3 33.02748722166663 34.0 33.0 34.0 31.0 34.0 4 36.40964954082202 37.0 37.0 37.0 35.0 37.0 5 36.254877793553874 37.0 37.0 37.0 35.0 37.0 6 36.31161520045575 37.0 37.0 37.0 35.0 37.0 7 36.30627993738103 37.0 37.0 37.0 35.0 37.0 8 36.293773634908305 37.0 37.0 37.0 35.0 37.0 9 38.1815806713458 39.0 39.0 39.0 37.0 39.0 10-11 38.08002874273322 39.0 38.0 39.0 37.0 39.0 12-13 38.11184059457513 39.0 38.0 39.0 37.0 39.0 14-15 39.54818500988769 41.0 39.0 41.0 37.0 41.0 16-17 39.52807211350817 41.0 39.0 41.0 37.0 41.0 18-19 39.568867798274496 41.0 39.5 41.0 37.0 41.0 20-21 39.57987881931463 41.0 39.5 41.0 37.0 41.0 22-23 39.51366829038817 41.0 39.0 41.0 37.0 41.0 24-25 39.46635477193209 41.0 39.0 41.0 37.0 41.0 26-27 39.42333816922728 41.0 39.0 41.0 37.0 41.0 28-29 39.328046540205776 40.0 39.0 41.0 36.0 41.0 30-31 39.23019130455656 40.0 39.0 41.0 36.0 41.0 32-33 39.12160384775118 40.0 39.0 41.0 35.5 41.0 34-35 39.044470272008475 40.0 39.0 41.0 35.0 41.0 36-37 39.042671881240025 40.0 39.0 41.0 35.0 41.0 38-39 39.03256753087105 40.0 39.0 41.0 35.0 41.0 40-41 38.97455660818134 40.0 38.5 41.0 35.0 41.0 42-43 38.92453630359448 40.0 38.0 41.0 35.0 41.0 44-45 38.87671292988325 40.0 38.0 41.0 35.0 41.0 46-47 38.78421091125696 40.0 38.0 41.0 35.0 41.0 48-49 38.7369025777397 40.0 38.0 41.0 35.0 41.0 50-51 38.62035872977753 40.0 38.0 41.0 35.0 41.0 52-53 38.42375270742269 40.0 37.5 41.0 34.0 41.0 54-55 38.25766552925191 40.0 37.0 41.0 34.0 41.0 56-57 38.065670833785404 40.0 37.0 41.0 34.0 41.0 58-59 37.84112005912144 39.0 36.0 41.0 33.5 41.0 60-61 37.67586752020499 39.0 36.0 41.0 33.0 41.0 62-63 37.46548349981627 39.0 35.0 40.5 33.0 41.0 64-65 37.174845966429245 38.5 35.0 40.0 33.0 41.0 66-67 36.8745745020353 37.5 35.0 40.0 33.0 41.0 68-69 36.59782477217147 37.0 35.0 40.0 33.0 41.0 70-71 36.291820891913375 36.5 35.0 39.0 32.5 41.0 72-73 35.94305835917404 36.0 35.0 39.0 32.0 41.0 74-75 35.57490775218641 35.5 34.5 38.5 32.0 40.0 76-77 33.701512677748546 34.5 32.0 36.0 29.0 39.0 78-79 34.794153081364286 35.0 34.0 37.0 31.0 39.0 80-81 34.75428230788104 35.0 34.0 36.5 31.0 39.0 82-83 34.51902403291495 35.0 34.0 36.0 31.0 37.5 84-85 34.31655065099827 35.0 34.0 36.0 31.0 37.0 86-87 34.14962915192395 35.0 34.0 35.0 31.0 37.0 88-89 34.02882281124573 35.0 34.0 35.0 31.0 36.0 90-91 33.913306263329275 35.0 34.0 35.0 31.0 36.0 92-93 33.85182969255037 35.0 34.0 35.0 31.0 36.0 94-95 33.78699393488834 35.0 34.0 35.0 31.0 36.0 96-97 33.75962420360858 35.0 34.0 35.0 31.0 35.0 98-99 33.79463500168056 35.0 34.0 35.0 31.0 35.0 100-101 32.48379617365762 34.0 31.5 35.0 28.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 10.0 20 35.0 21 140.0 22 515.0 23 1730.0 24 4653.0 25 9989.0 26 18786.0 27 30885.0 28 47030.0 29 66529.0 30 89424.0 31 119719.0 32 162362.0 33 229754.0 34 364268.0 35 609369.0 36 1237268.0 37 2557944.0 38 2991798.0 39 1263243.0 40 27989.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54439676361543 19.15442222570693 12.55920358303837 24.741977427639274 2 15.67951624571792 20.82416477193799 39.37313988965387 24.123179092690215 3 18.884127242570834 25.465792776483436 29.639975401117486 26.010104579828248 4 13.03675806437507 16.855861339995773 37.505809262562806 32.60157133306635 5 13.70644872184568 37.51374010938393 35.04788813921318 13.731923029557214 6 31.64647739824977 36.56411457961515 18.09020686754445 13.699201154590632 7 27.6499317329578 31.876468076996844 22.622607628132652 17.850992561912708 8 25.865762400231457 35.568372437365156 20.70037937088805 17.865485791515333 9 26.50294955416767 15.711650177089185 20.376661428280432 37.40873884046271 10-11 25.312122791933717 25.81324400195478 28.258916252290227 20.615716953821277 12-13 25.755148581430916 23.421904654157448 28.492102817816296 22.330843946595344 14-15 22.864834114817334 24.697038509866548 25.74377928022659 26.694348095089527 16-17 22.775511387066707 27.80280383858733 26.406045576741494 23.015639197604468 18-19 22.84970511851293 27.18469789113517 27.853258299586248 22.112338690765654 20-21 23.467933281528918 26.439792013516744 27.684918315504337 22.40735638945 22-23 23.376606998264464 26.325788347094694 27.56094669844113 22.73665795619971 24-25 23.01651694158621 26.51515328760592 27.62537850031733 22.84295127049054 26-27 22.95586551728682 26.613361258347375 27.576151485806765 22.854621738559043 28-29 22.935833764258657 26.723966455079236 27.54621117236414 22.79398860829797 30-31 23.145046567335257 26.58328939867819 27.427767857780204 22.84389617620635 32-33 22.900211946330785 26.807993185413043 27.430481066800795 22.86131380145538 34-35 23.045097005231533 26.770382968076294 27.457770506277097 22.726749520415076 36-37 23.069222340694342 26.678415218673766 27.406294258581937 22.846068182049954 38-39 22.971809104636453 26.80799538751578 27.460787609269317 22.75940789857845 40-41 23.12435805957333 26.685217578902133 27.362187903870776 22.828236457653762 42-43 22.9827352327761 26.798069724437735 27.215107950167194 23.004087092618974 44-45 23.055758809255796 26.876011568545504 27.088911807836975 22.97931781436173 46-47 23.19202395662995 26.86453780100202 26.877068273504534 23.066369968863494 48-49 23.083412975955167 27.00815612849975 26.80275961684659 23.105671278698495 50-51 23.044913007493317 27.09043719610663 26.768789485600102 23.095860310799946 52-53 23.241948272253758 26.977382630733636 26.599772065767645 23.180897031244953 54-55 23.154448752879066 26.8770771511943 26.69125936901901 23.277214726907623 56-57 23.154962379611906 26.941938919561952 26.737370451596597 23.16572824922954 58-59 23.12127577093718 27.047233372746337 26.66923964495183 23.162251211364648 60-61 23.20580393995603 26.984653567262562 26.699141895128236 23.110400597653168 62-63 23.184962681791884 27.0769186617406 26.62464020188942 23.113478454578086 64-65 23.20519077274544 27.026421851368536 26.557060896155075 23.21132647973095 66-67 23.24040643037824 26.998313581978934 26.471500882242328 23.289779105400502 68-69 23.12349560932702 26.997741887933614 26.616735591686552 23.262026911052814 70-71 23.15292663372752 26.849776263558724 26.600633808058905 23.396663294654854 72-73 23.198306954670088 26.937144204161694 26.55468152508182 23.309867316086393 74-75 23.013657206447625 27.081466666924804 26.602496746403183 23.302379380224387 76-77 23.234565205275974 26.959897658041832 26.500139831789877 23.305397304892317 78-79 23.144362953691473 26.972884990088858 26.48851297580577 23.3942390804139 80-81 23.177176590174156 27.043383580260848 26.503445598903753 23.275994230661244 82-83 23.175352300574364 26.961686736443724 26.625441349542406 23.237519613439503 84-85 23.230319251218084 26.98651014238427 26.52427686173419 23.258893744663453 86-87 23.128353890060165 26.989806346614074 26.649426039937534 23.23241372338822 88-89 23.121453155151993 26.953284343484015 26.570099179942446 23.355163321421543 90-91 23.18552692433657 26.973262376741552 26.575964104572737 23.265246594349144 92-93 23.24354335876495 26.963657794012626 26.572487686022573 23.22031116119986 94-95 23.072184350257274 26.95126902814876 26.672602389435042 23.303944232158923 96-97 23.162198990556572 26.95973518176481 26.618909146097714 23.259156681580905 98-99 23.365964341442186 27.62239046533461 26.659286233289915 22.352358959933287 100-101 24.24019191821158 30.032634817968862 23.595071727764136 22.132101536055423 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2187.0 1 1930.5 2 2557.5 3 3696.0 4 4891.0 5 7742.5 6 10250.5 7 12103.0 8 13437.5 9 13149.5 10 11528.5 11 9853.0 12 10235.5 13 13371.5 14 20826.0 15 30244.5 16 37431.5 17 41475.0 18 42256.0 19 40175.0 20 36681.0 21 33746.5 22 33274.5 23 36850.5 24 43785.5 25 54684.0 26 68909.5 27 83696.0 28 100793.5 29 120738.0 30 139700.0 31 158362.5 32 177228.0 33 194546.0 34 210845.5 35 226744.5 36 245022.5 37 263749.5 38 280942.5 39 295163.0 40 308026.5 41 323577.5 42 338623.0 43 352562.5 44 367205.5 45 383898.0 46 398615.5 47 410454.0 48 420073.5 49 423194.0 50 424050.5 51 425363.0 52 422547.0 53 416616.5 54 408689.0 55 392837.5 56 371891.0 57 346489.5 58 314653.5 59 277435.0 60 236217.5 61 198147.5 62 162831.5 63 129162.0 64 98875.5 65 74889.0 66 57356.0 67 44267.0 68 34375.0 69 25624.5 70 18896.5 71 13916.0 72 10031.0 73 7224.0 74 5172.0 75 3657.5 76 2483.0 77 1608.5 78 1085.0 79 747.5 80 501.0 81 336.5 82 245.5 83 181.0 84 129.5 85 93.0 86 65.5 87 46.0 88 34.5 89 27.0 90 18.5 91 11.5 92 8.0 93 3.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 2.0338758290332112E-4 3 0.004830455093953877 4 8.135503316132846E-5 5 5.084689572583028E-5 6 0.0 7 0.0 8 5.796546112744653E-4 9 0.0026542079568883407 10-11 9.406675709278603E-4 12-13 2.9999668478239866E-4 14-15 1.423713080323248E-4 16-17 0.0013932049428877497 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 6.781357263672376E-4 32-33 7.973800544886593E-4 34-35 6.15262919534326E-5 36-37 8.03465946074456E-4 38-39 6.637817742741625E-4 40-41 3.6583372251073145E-5 42-43 1.369720196192399E-4 44-45 9.675981677518564E-4 46-47 0.0 48-49 5.104341981355685E-4 50-51 3.2915227654057666E-5 52-53 2.2152114471937616E-5 54-55 7.603281352605863E-4 56-57 8.867925189053432E-4 58-59 1.0847603784306475E-4 60-61 1.096588938903605E-4 62-63 1.166613119124499E-5 64-65 5.894050551267601E-6 66-67 0.0 68-69 0.0 70-71 3.9658606512492313E-4 72-73 0.0 74-75 1.8735618071178113E-5 76-77 0.0 78-79 3.069643299774458E-4 80-81 0.0 82-83 1.3121001415821658E-5 84-85 1.3284281566458571E-5 86-87 2.4212415521368967E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 193.0 20-21 606.0 22-23 1158.0 24-25 3218.0 26-27 5034.0 28-29 11577.0 30-31 23081.0 32-33 28268.0 34-35 38232.0 36-37 61524.0 38-39 74919.0 40-41 75165.0 42-43 82051.0 44-45 97891.0 46-47 98550.0 48-49 95667.0 50-51 87171.0 52-53 83526.0 54-55 90186.0 56-57 94098.0 58-59 94490.0 60-61 92692.0 62-63 88665.0 64-65 91312.0 66-67 96126.0 68-69 98270.0 70-71 98321.0 72-73 92385.0 74-75 93187.0 76-77 92797.0 78-79 98425.0 80-81 99341.0 82-83 94496.0 84-85 92892.0 86-87 96606.0 88-89 102743.0 90-91 110292.0 92-93 108206.0 94-95 118697.0 96-97 181834.0 98-99 816449.0 100-101 5923101.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.65785110775562 #Duplication Level Percentage of deduplicated Percentage of total 1 88.28835699583853 72.97725867110395 2 7.879655987855909 13.026308628490574 3 2.080025359856231 5.1579127948605645 4 0.7945571914536705 2.627055601110959 5 0.3747939845605643 1.548983268594479 6 0.1971786306312449 0.977901713540915 7 0.11806453138797023 0.6831272319601649 8 0.07084031457755514 0.46844065398224977 9 0.048343505426015405 0.3596373246177501 >10 0.1437492163978133 1.8709581100038575 >50 0.003571489644985125 0.19753737724586187 >100 8.40407592229817E-4 0.09318487013831263 >500 2.23847772750017E-5 0.01169375435032893 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.067751658066423E-5 0.0 0.0 0.0 3 0.0 4.067751658066423E-5 0.0 0.0 0.0 4 0.0 7.11856540161624E-5 0.0 0.0 0.0 5 2.0338758290332114E-5 8.135503316132846E-5 0.0 0.0 0.0 6 3.050813743549817E-5 8.135503316132846E-5 0.0 0.0 2.0338758290332114E-5 7 3.050813743549817E-5 8.135503316132846E-5 0.0 0.0 2.0338758290332114E-5 8 3.050813743549817E-5 9.152441230649452E-5 0.0 0.0 3.050813743549817E-5 9 3.050813743549817E-5 2.0338758290332115E-4 0.0 0.0 3.050813743549817E-5 10-11 3.050813743549817E-5 2.0338758290332115E-4 0.0 0.0 3.050813743549817E-5 12-13 3.050813743549817E-5 2.0338758290332115E-4 0.0 0.0 3.050813743549817E-5 14-15 4.067751658066423E-5 3.1525075350014774E-4 0.0 0.0 6.101627487099634E-5 16-17 5.084689572583029E-5 3.1525075350014774E-4 0.0 0.0 8.135503316132846E-5 18-19 5.084689572583029E-5 3.4575889093564594E-4 0.0 1.0169379145166057E-5 9.152441230649452E-5 20-21 5.084689572583029E-5 3.762670283711441E-4 0.0 1.0169379145166057E-5 9.660910187907754E-5 22-23 6.101627487099634E-5 4.169445449518083E-4 0.0 1.0169379145166057E-5 1.067784810242436E-4 24-25 8.135503316132846E-5 4.3728330324214044E-4 0.0 3.050813743549817E-5 1.1694786016940965E-4 26-27 8.135503316132846E-5 4.525373719598895E-4 0.0 4.576220615324725E-5 1.3220192888715874E-4 28-29 8.135503316132846E-5 4.6270675110505555E-4 0.0 4.423679928147235E-4 1.3728661845974178E-4 30-31 9.152441230649452E-5 5.38977094693801E-4 0.0 0.0017135403859604805 1.474559976049078E-4 32-33 9.660910187907754E-5 5.38977094693801E-4 0.0 0.0040524975893486736 1.627100663226569E-4 34-35 1.1186317059682662E-4 6.559249548632106E-4 0.0 0.007128734780761406 2.0338758290332115E-4 36-37 1.2203254974199268E-4 7.423646775971222E-4 0.0 0.011633769742069968 2.0338758290332115E-4 38-39 1.2203254974199268E-4 7.779575046052033E-4 0.0 0.01839132218403281 2.135569620484872E-4 40-41 1.2203254974199268E-4 9.152441230649451E-4 0.0 0.025886154614020197 2.135569620484872E-4 42-43 1.2203254974199268E-4 9.610063292181923E-4 0.0 0.03279116305358795 2.1864165162107022E-4 44-45 1.2203254974199268E-4 0.001077954189387602 0.0 0.04054022996220449 2.2372634119365324E-4 46-47 1.2203254974199268E-4 0.0011847326704118456 0.0 0.048782511759361574 2.2372634119365324E-4 48-49 1.2203254974199268E-4 0.0011898173599844287 0.0 0.05675022031959918 2.338957203388193E-4 50-51 1.2203254974199268E-4 0.001301680530581255 0.0 0.06511961935607084 2.338957203388193E-4 52-53 1.2203254974199268E-4 0.0013118499097264213 0.0 0.07287885564383254 2.5423447862915144E-4 54-55 1.2203254974199268E-4 0.0013474427367345025 0.0 0.08075503979176366 2.847426160646496E-4 56-57 1.2203254974199268E-4 0.0013626968054522516 0.0 0.08826512629046879 2.949119952098156E-4 58-59 1.2203254974199268E-4 0.0013677814950248347 0.0 0.09570402713515776 2.9999668478239866E-4 60-61 1.3220192888715874E-4 0.00139320494288775 0.0 0.10235480109609636 3.1525075350014774E-4 62-63 1.3220192888715874E-4 0.001413543701178082 0.0 0.10910726884848662 3.1525075350014774E-4 64-65 1.3220192888715874E-4 0.0015711690779281558 0.0 0.11535126764361858 3.1525075350014774E-4 66-67 1.3220192888715874E-4 0.001606761904936237 0.0 0.12161052050746829 3.3050482221789687E-4 68-69 1.3220192888715874E-4 0.0016372700423717352 0.0 0.12820027819353588 3.4575889093564594E-4 70-71 1.3220192888715874E-4 0.0016525241110894842 0.0 0.13459173298627275 3.55928270080812E-4 72-73 1.3220192888715874E-4 0.0016677781798072333 0.0 0.1399154029687672 4.067751658066423E-4 74-75 1.3220192888715874E-4 0.0016932016276701486 0.0 0.14442552261964833 4.067751658066423E-4 76-77 1.3220192888715874E-4 0.0017593025921137278 0.0 0.14826446324694853 4.067751658066423E-4 78-79 1.3220192888715874E-4 0.00177964135040406 0.0 0.15190510098091797 4.067751658066423E-4 80-81 1.3220192888715874E-4 0.001789810729549226 0.0 0.1535474557128623 4.2202923452439137E-4 82-83 1.3728661845974178E-4 0.0018101494878395583 0.0 0.15413727970328192 4.3728330324214044E-4 84-85 1.423713080323248E-4 0.0018203188669847243 0.0 0.15470676493541125 4.3728330324214044E-4 86-87 1.423713080323248E-4 0.0018457423148476393 0.0 0.15501693099933878 4.3728330324214044E-4 88-89 1.423713080323248E-4 0.0019118432792912187 0.0 0.15509320134292753 4.423679928147235E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10560 0.0 45.77499 1 GTATCAA 18025 0.0 35.040173 1 TCAACGC 21510 0.0 28.265963 4 ATCAACG 21650 0.0 28.04557 3 CAACGCA 21980 0.0 27.642393 5 TATCAAC 22525 0.0 27.516504 2 AACGCAG 22785 0.0 26.776659 6 GTACATG 29420 0.0 24.91761 1 TACATGG 29950 0.0 24.26578 2 ACATGGG 30715 0.0 23.277914 3 ACGCAGA 27150 0.0 22.39414 7 CGCAGAG 27770 0.0 21.894163 8 CATGGGG 24220 0.0 21.104818 4 GAGTACT 17745 0.0 18.377144 12-13 GCAGAGT 33625 0.0 18.207031 9 ATGGGGG 16330 0.0 17.19795 5 CAGAGTA 31580 0.0 17.079239 10-11 AGAGTAC 28765 0.0 17.02342 10-11 GAGTACA 20600 0.0 16.944845 1 GTACTTT 20065 0.0 16.200459 14-15 >>END_MODULE