FastQCFastQC Report
Fri 27 May 2016
SRR2140007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7907827
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75800.043.637721
GTATCAA134700.035.373561
TCAACGC156700.029.605114
ATCAACG158650.029.2425213
CAACGCA161050.028.7536545
AACGCAG167700.027.6885286
TATCAAC172550.027.3473872
GTACATG220300.023.852471
TACATGG221800.023.2193742
ACGCAGA200400.023.1288497
CGCAGAG201450.022.9667288
ACATGGG228950.022.2386323
CATGGGG164800.020.5535684
GCAGAGT245250.018.7795459
AGAGTAC219500.017.36771610-11
GAGTACT139750.016.76276212-13
ATGGGGG106850.016.5754175
CAGAGTA241500.016.21032710-11
GAGTACA155750.016.0759741
AGTACTT157250.014.96383512-13
Produced by FastQC (version 0.11.5)