##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140007_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7907827
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75464245740328	34.0	31.0	34.0	31.0	34.0
2	32.93346326367534	34.0	31.0	34.0	31.0	34.0
3	33.02984852855279	34.0	33.0	34.0	31.0	34.0
4	36.41299955095123	37.0	37.0	37.0	35.0	37.0
5	36.259897820222925	37.0	37.0	37.0	35.0	37.0
6	36.314838071192	37.0	37.0	37.0	35.0	37.0
7	36.31518102254893	37.0	37.0	37.0	35.0	37.0
8	36.30108347084477	37.0	37.0	37.0	35.0	37.0
9	38.19846362344548	39.0	39.0	39.0	37.0	39.0
10-11	38.09783149024378	39.0	38.0	39.0	37.0	39.0
12-13	38.13450205726554	39.0	38.0	39.0	37.0	39.0
14-15	39.57417397977978	41.0	39.0	41.0	37.0	41.0
16-17	39.55741665820459	41.0	39.0	41.0	37.0	41.0
18-19	39.60471214152763	41.0	39.5	41.0	37.0	41.0
20-21	39.619539464778924	41.0	40.0	41.0	37.0	41.0
22-23	39.55690302737954	41.0	39.0	41.0	37.0	41.0
24-25	39.51482086908829	41.0	39.0	41.0	37.0	41.0
26-27	39.47847317839601	41.0	39.0	41.0	37.0	41.0
28-29	39.38382674132089	40.5	39.0	41.0	36.5	41.0
30-31	39.29205441349727	40.0	39.0	41.0	36.0	41.0
32-33	39.18829365364515	40.0	39.0	41.0	36.0	41.0
34-35	39.11686272113981	40.0	39.0	41.0	35.0	41.0
36-37	39.11976415448893	40.0	39.0	41.0	35.0	41.0
38-39	39.111088295211275	40.0	39.0	41.0	35.0	41.0
40-41	39.06535261214741	40.0	39.0	41.0	35.0	41.0
42-43	39.02765869690131	40.0	39.0	41.0	35.0	41.0
44-45	38.9909985454923	40.0	38.5	41.0	35.0	41.0
46-47	38.916838926534325	40.0	38.0	41.0	35.0	41.0
48-49	38.884678314062896	40.0	38.0	41.0	35.0	41.0
50-51	38.790372937788106	40.0	38.0	41.0	35.0	41.0
52-53	38.61689446741039	40.0	38.0	41.0	35.0	41.0
54-55	38.47963313953659	40.0	38.0	41.0	34.5	41.0
56-57	38.31517730954888	40.0	37.0	41.0	34.0	41.0
58-59	38.12492345270401	40.0	37.0	41.0	34.0	41.0
60-61	37.99285997183978	39.5	36.5	41.0	34.0	41.0
62-63	37.81586828937955	39.0	36.0	41.0	34.0	41.0
64-65	37.55847916231308	39.0	35.5	41.0	33.5	41.0
66-67	37.28474270015685	39.0	35.0	40.0	33.0	41.0
68-69	37.02734623200615	38.0	35.0	40.0	33.0	41.0
70-71	36.73047413923828	37.0	35.0	40.0	33.0	41.0
72-73	36.381426437010106	37.0	35.0	39.0	33.0	41.0
74-75	36.008087797766926	36.0	35.0	39.0	32.0	40.5
76-77	34.086460887378244	34.5	32.5	36.5	29.0	39.0
78-79	35.16017269880672	35.0	34.0	37.0	31.5	39.0
80-81	35.09128447398774	35.0	34.0	37.0	32.0	39.0
82-83	34.820590512254626	35.0	34.0	36.5	32.0	39.0
84-85	34.58167801196328	35.0	34.0	36.0	31.5	37.0
86-87	34.37658770874616	35.0	34.0	36.0	31.0	37.0
88-89	34.22254314809804	35.0	34.0	35.5	31.0	37.0
90-91	34.08367440301325	35.0	34.0	35.0	31.0	36.0
92-93	33.999427806332335	35.0	34.0	35.0	31.0	36.0
94-95	33.919795647491874	35.0	34.0	35.0	31.0	36.0
96-97	33.87888772900419	35.0	34.0	35.0	31.0	36.0
98-99	33.901689534905984	35.0	34.0	35.0	31.0	35.0
100-101	32.65153006392579	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	24.0
21	73.0
22	343.0
23	1207.0
24	3196.0
25	7217.0
26	13454.0
27	22551.0
28	34096.0
29	48600.0
30	65955.0
31	89105.0
32	120508.0
33	167835.0
34	261011.0
35	432478.0
36	865233.0
37	1888276.0
38	2611663.0
39	1247061.0
40	27934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65060426840395	19.903723235219992	12.933034574479182	24.512637921896875
2	15.0265313557617	21.426568888262786	40.411438661991625	23.135461093983885
3	18.354534991493495	26.15641470562236	30.323077683797834	25.165972619086315
4	12.382366531585292	17.5454796299261	39.08830315689799	30.983850681590617
5	13.009961651908078	38.05142374799645	35.869660747424234	13.068953852671248
6	30.168844108501613	38.02724819346706	18.781872693977753	13.022035004053578
7	26.542133508990524	32.50817196683741	23.51971028197759	17.429984242194475
8	24.750236065377244	36.463884178681546	21.516036069348022	17.269843686593195
9	25.903232410936933	16.128239230302594	21.117751254579915	36.85077710418056
10-11	24.294177045191713	26.588064501239607	29.237621802901074	19.880136650667605
12-13	24.921898261266147	24.163159525705936	29.302740479662187	21.612201733365733
14-15	22.023179345599342	25.564656537279067	26.679148831991036	25.733015285130563
16-17	21.802417337615402	28.665436305943516	27.36385182659754	22.16829452984354
18-19	21.826704099621804	28.090953431328224	28.81565947257066	21.26668299647931
20-21	22.5773520828242	27.366262234681415	28.61277310382395	21.443612578670436
22-23	22.2968716584337	27.314556547316798	28.44811701177926	21.94045478247024
24-25	22.047203407662764	27.3472538972243	28.599044638578413	22.00649805653452
26-27	22.021261088720394	27.564735553599633	28.456704705863896	21.957298651816078
28-29	21.93356024513362	27.719032496296954	28.45038687816234	21.89702038040709
30-31	22.22603605824873	27.534342117617733	28.382374692821184	21.857247131312356
32-33	21.98560873234144	27.702885143685123	28.29997032911797	22.011535794855472
34-35	22.104529060344998	27.705460770833174	28.35257051312056	21.837439655701267
36-37	22.113413903108018	27.618819378653058	28.337219141307457	21.93054757693147
38-39	21.953603466670284	27.771893715111112	28.382188264618886	21.892314553599725
40-41	22.162090588072243	27.61319279652332	28.292918915014877	21.93179770038956
42-43	22.068239727085707	27.722045492495283	28.121070123738512	22.088644656680497
44-45	22.106359569801523	27.8595633093813	27.993447176355463	22.040629944461713
46-47	22.183485010661812	27.759674089398466	27.820537493658946	22.236303406280776
48-49	22.15421335691255	27.86883191782404	27.798873169035144	22.178081556228268
50-51	22.132238985788998	27.982866022288544	27.709742340023546	22.17515265189891
52-53	22.30380757965127	27.882454033656444	27.585254017589335	22.22848436910295
54-55	22.237604313565154	27.790980771842694	27.686451605244404	22.284963309347745
56-57	22.256834892621118	27.88976700540371	27.61298877710907	22.240409324866107
58-59	22.220471733852225	27.96872020843486	27.579985678990525	22.230822378722387
60-61	22.284957854891903	27.911143143187083	27.618327385562143	22.185571616358867
62-63	22.2697601762502	27.951348378887708	27.564348174753807	22.214543270108283
64-65	22.251505779560134	27.935391856951973	27.552090437111527	22.26101192637637
66-67	22.292756013710814	27.91008939890552	27.46551000871017	22.331644578673494
68-69	22.262755050181195	27.930729144270476	27.525880329468382	22.280635476079944
70-71	22.238794241252293	27.736173014939382	27.567252687165382	22.457780056642942
72-73	22.299777365589375	27.797775267053627	27.53872709383782	22.363720273519178
74-75	22.138446314675654	27.933055737897654	27.496887338708888	22.431610608717804
76-77	22.280475315621924	27.84996735957875	27.44277136059303	22.426785964206296
78-79	22.283901091975476	27.877922593112185	27.415937602502993	22.422238712409342
80-81	22.225843463402818	27.91717837951881	27.433162053328402	22.42381610374997
82-83	22.323753136859878	27.83507552464925	27.512312788457905	22.32885855003297
84-85	22.387386352673094	27.838314972817358	27.422562781938716	22.35173589257083
86-87	22.21512134795258	27.860843810624903	27.49700133078638	22.42703351063614
88-89	22.28746710402163	27.83903015621944	27.46321826460204	22.41028447515689
90-91	22.328413565239348	27.873933209849405	27.481306549944605	22.316346674966645
92-93	22.33937150308159	27.82695466063796	27.494983073869466	22.338690762410984
94-95	22.202615615696782	27.89022068342638	27.52112938363827	22.386034317238565
96-97	22.257565086252733	27.87567544882334	27.57350813345577	22.293251331468163
98-99	22.493985900886276	28.50596446104372	27.592300659882035	21.407748978187968
100-101	26.55325401116615	35.518860137143434	28.22050474134783	9.707381110342588
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1630.0
1	1415.5
2	1891.0
3	2798.0
4	3844.0
5	6106.0
6	8029.5
7	9446.5
8	10252.0
9	9850.5
10	8864.0
11	7921.0
12	8484.0
13	11066.0
14	16929.0
15	24672.5
16	30695.0
17	34200.0
18	35198.0
19	34163.0
20	32579.0
21	31703.0
22	32890.0
23	37467.0
24	45791.5
25	57639.0
26	72597.5
27	89188.5
28	107602.5
29	127373.5
30	144422.5
31	160754.5
32	178243.0
33	195139.0
34	211753.5
35	226918.0
36	243649.5
37	258321.0
38	269129.5
39	279025.0
40	289970.0
41	300777.5
42	308229.5
43	315805.5
44	323830.0
45	330202.0
46	332754.0
47	333822.5
48	334911.0
49	332530.0
50	327477.5
51	319952.5
52	307231.0
53	292658.0
54	277362.5
55	259022.0
56	239940.5
57	218085.0
58	193717.0
59	169874.5
60	144820.5
61	119400.5
62	95688.5
63	75233.0
64	57834.5
65	43923.5
66	33441.5
67	25791.0
68	19854.0
69	14276.5
70	9937.0
71	7237.0
72	5252.5
73	3675.5
74	2563.5
75	1753.0
76	1206.5
77	820.5
78	526.5
79	354.5
80	258.0
81	186.5
82	136.5
83	81.5
84	44.5
85	28.0
86	21.0
87	20.5
88	21.0
89	18.0
90	13.0
91	9.0
92	6.5
93	3.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.7703978602465634E-4
3	0.004400703252612886
4	1.2645699001761167E-5
5	2.5291398003522333E-5
6	0.0
7	0.0
8	7.587419401056699E-4
9	0.0025291398003522334
10-11	9.104903281268041E-4
12-13	4.109852175572379E-4
14-15	1.3910268901937283E-4
16-17	0.001504838181209579
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	7.290896763818183E-4
32-33	8.135908314415228E-4
34-35	1.2754070652959952E-5
36-37	8.458248961765959E-4
38-39	6.841386204254361E-4
40-41	3.9040397441656726E-5
42-43	7.217172461421917E-5
44-45	0.0010201833335696095
46-47	0.0
48-49	6.772995929768096E-4
50-51	2.738149903302236E-5
52-53	1.382711337921861E-5
54-55	7.052823412803989E-4
56-57	0.0010517335003307313
58-59	1.3562895572556924E-4
60-61	1.4440406104652802E-4
62-63	3.650461852783152E-5
64-65	0.0
66-67	7.465648312420062E-6
68-69	0.0
70-71	3.6728329195402437E-4
72-73	0.0
74-75	1.5675975086799835E-5
76-77	0.0
78-79	4.418429445956261E-4
80-81	0.0
82-83	8.247839663976414E-6
84-85	2.505889257403107E-5
86-87	3.046042970189731E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.7959333065799135E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	161.0
20-21	445.0
22-23	993.0
24-25	2420.0
26-27	3811.0
28-29	9074.0
30-31	19020.0
32-33	24116.0
34-35	32752.0
36-37	52143.0
38-39	63142.0
40-41	62611.0
42-43	69603.0
44-45	82152.0
46-47	82972.0
48-49	79603.0
50-51	73045.0
52-53	70932.0
54-55	75724.0
56-57	79021.0
58-59	79294.0
60-61	77688.0
62-63	74008.0
64-65	75825.0
66-67	80795.0
68-69	81424.0
70-71	80933.0
72-73	76089.0
74-75	77328.0
76-77	76484.0
78-79	81094.0
80-81	81534.0
82-83	76784.0
84-85	76285.0
86-87	78931.0
88-89	83744.0
90-91	88826.0
92-93	86512.0
94-95	93818.0
96-97	143194.0
98-99	639668.0
100-101	4713829.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.27058644082696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.55389655060479	76.36313276931222
2	7.288598190535156	12.43006084076966
3	1.8320370290043217	4.686566155335265
4	0.624208791194334	2.1290659874664226
5	0.2857032662070622	1.2181042528767945
6	0.14743403817015074	0.7543072137664811
7	0.0914255208097099	0.5457135442571394
8	0.04678188358192802	0.31912949182699973
9	0.03400999913905328	0.2610047314295184
>10	0.09459500774361165	1.1755279310281677
>50	0.0010170318617304113	0.05696986899006719
>100	2.6975653503024776E-4	0.04324662425545311
>500	2.293461316175975E-5	0.017170588685769574
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.79370970052835E-5	0.0	1.2645699001761167E-5	0.0
3	0.0	3.79370970052835E-5	0.0	2.5291398003522333E-5	0.0
4	0.0	5.058279600704467E-5	0.0	2.5291398003522333E-5	0.0
5	0.0	5.058279600704467E-5	0.0	2.5291398003522333E-5	0.0
6	0.0	5.058279600704467E-5	0.0	2.5291398003522333E-5	2.5291398003522333E-5
7	0.0	6.322849500880583E-5	0.0	2.5291398003522333E-5	2.5291398003522333E-5
8	0.0	6.322849500880583E-5	0.0	2.5291398003522333E-5	3.79370970052835E-5
9	0.0	1.51748388021134E-4	0.0	3.79370970052835E-5	5.058279600704467E-5
10-11	0.0	1.51748388021134E-4	0.0	6.322849500880583E-5	5.058279600704467E-5
12-13	0.0	1.5807123752201456E-4	0.0	8.219704351144759E-5	5.058279600704467E-5
14-15	0.0	2.8452822753962623E-4	0.0	9.484274251320875E-5	8.851989301232816E-5
16-17	0.0	3.03496776042268E-4	0.0	1.0116559201408933E-4	1.138112910158505E-4
18-19	0.0	3.0981962554314855E-4	0.0	1.2645699001761167E-4	1.2645699001761167E-4
20-21	0.0	3.161424750440292E-4	0.0	1.51748388021134E-4	1.2645699001761167E-4
22-23	0.0	3.161424750440292E-4	0.0	1.833626355255369E-4	1.4542553852025343E-4
24-25	0.0	3.4775672254843207E-4	0.0	2.4026828103346215E-4	1.6439408702289515E-4
26-27	0.0	3.667252710510738E-4	0.0	2.7188252853786504E-4	1.6439408702289515E-4
28-29	0.0	3.667252710510738E-4	0.0	6.196392510862971E-4	1.6439408702289515E-4
30-31	0.0	4.109852175572379E-4	0.0	0.001884209151262414	1.7703978602465632E-4
32-33	0.0	4.1730806705811847E-4	0.0	0.003977072336053887	2.0233118402817867E-4
34-35	0.0	4.615680135642826E-4	0.0	0.007208048431003864	2.0233118402817867E-4
36-37	1.2645699001761167E-5	5.058279600704467E-4	0.0	0.011216735014562156	2.0233118402817867E-4
38-39	1.2645699001761167E-5	5.31119358073969E-4	0.0	0.017855726990486768	2.1497688302993983E-4
40-41	1.2645699001761167E-5	6.196392510862971E-4	0.0	0.025133326766000316	2.27622582031701E-4
42-43	1.2645699001761167E-5	6.512534985907E-4	0.0	0.03228446955149626	2.4026828103346215E-4
44-45	1.2645699001761167E-5	7.5874194010567E-4	0.0	0.039701172016029185	2.5291398003522334E-4
46-47	1.2645699001761167E-5	8.346161341162369E-4	0.0	0.048919886588313075	2.592368295361039E-4
48-49	1.2645699001761167E-5	8.346161341162369E-4	0.0	0.05794891567557055	3.03496776042268E-4
50-51	1.2645699001761167E-5	9.990102211391322E-4	0.0	0.0664341797057523	3.03496776042268E-4
52-53	1.2645699001761167E-5	9.990102211391322E-4	0.0	0.07419863889283365	3.7304812055195444E-4
54-55	1.2645699001761167E-5	0.0010116559201408934	0.0	0.08159004995936306	4.362766155607602E-4
56-57	1.2645699001761167E-5	0.0010369473181444156	0.0	0.08999943979553422	4.4259946506164084E-4
58-59	1.2645699001761167E-5	0.0010369473181444156	0.0	0.0982760497921869	4.5524516406340203E-4
60-61	1.2645699001761167E-5	0.00108120726465058	0.0	0.10608476892577443	4.6789086306516317E-4
62-63	1.2645699001761167E-5	0.001138112910158505	0.0	0.11398200795237427	4.805365620669243E-4
64-65	1.2645699001761167E-5	0.0013530897931884449	0.0	0.12159471875143449	4.805365620669243E-4
66-67	1.2645699001761167E-5	0.001365735492190206	0.0	0.1300989513301189	4.805365620669243E-4
68-69	1.2645699001761167E-5	0.0013910268901937283	0.0	0.1382238129387504	4.805365620669243E-4
70-71	1.2645699001761167E-5	0.0014036725891954894	0.0	0.14581123233980714	5.184736590722078E-4
72-73	1.2645699001761167E-5	0.0014036725891954894	0.0	0.1516788366766243	5.943478530827748E-4
74-75	1.2645699001761167E-5	0.0014352868366998923	0.0	0.15666124208331822	5.943478530827748E-4
76-77	1.2645699001761167E-5	0.0014732239337051757	0.0	0.16078373995789236	5.943478530827748E-4
78-79	1.2645699001761167E-5	0.0014795467832060564	0.0	0.16440040987239604	5.943478530827748E-4
80-81	1.2645699001761167E-5	0.0014795467832060564	0.0	0.16612654778613645	6.006707025836554E-4
82-83	1.2645699001761167E-5	0.0014795467832060564	0.0	0.16687896687674125	6.196392510862971E-4
84-85	1.2645699001761167E-5	0.0014921924822078176	0.0	0.16737847198731082	6.196392510862971E-4
86-87	1.2645699001761167E-5	0.0014921924822078176	0.0	0.1676756459138522	6.196392510862971E-4
88-89	1.2645699001761167E-5	0.001593358074221907	0.0	0.1677704886563654	6.196392510862971E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7580	0.0	43.63772	1
GTATCAA	13470	0.0	35.37356	1
TCAACGC	15670	0.0	29.60511	4
ATCAACG	15865	0.0	29.242521	3
CAACGCA	16105	0.0	28.753654	5
AACGCAG	16770	0.0	27.688528	6
TATCAAC	17255	0.0	27.347387	2
GTACATG	22030	0.0	23.85247	1
TACATGG	22180	0.0	23.219374	2
ACGCAGA	20040	0.0	23.128849	7
CGCAGAG	20145	0.0	22.966728	8
ACATGGG	22895	0.0	22.238632	3
CATGGGG	16480	0.0	20.553568	4
GCAGAGT	24525	0.0	18.779545	9
AGAGTAC	21950	0.0	17.367716	10-11
GAGTACT	13975	0.0	16.762762	12-13
ATGGGGG	10685	0.0	16.575417	5
CAGAGTA	24150	0.0	16.210327	10-11
GAGTACA	15575	0.0	16.075974	1
AGTACTT	15725	0.0	14.963835	12-13
>>END_MODULE