FastQCFastQC Report
Fri 27 May 2016
SRR2140006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8649139
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87850.044.3986241
GTATCAA148450.033.6935771
TCAACGC174450.027.7462274
ATCAACG176050.027.4711723
CAACGCA179250.026.9339315
TATCAAC189400.025.9277672
AACGCAG186750.025.8965916
TACATGG261750.021.8774072
GTACATG269250.021.8370021
ACGCAGA222700.021.753477
CGCAGAG225200.021.4752068
ACATGGG266800.020.9516563
CATGGGG189200.019.1052844
GCAGAGT276800.017.5616349
GAGTACT143000.017.21367812-13
GAGTACA188800.016.7552811
AGAGTAC244350.016.39434210-11
CAGAGTA266150.016.249410-11
AGTACAT198650.015.2783272
GTGGTAT50450.015.1013941
Produced by FastQC (version 0.11.5)