##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8649139
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70278983838738	34.0	31.0	34.0	31.0	34.0
2	32.89823310736479	34.0	31.0	34.0	31.0	34.0
3	32.99849418537498	34.0	31.0	34.0	31.0	34.0
4	36.39577222657654	37.0	37.0	37.0	35.0	37.0
5	36.222893862614534	37.0	37.0	37.0	35.0	37.0
6	36.274393324006006	37.0	37.0	37.0	35.0	37.0
7	36.28170989042955	37.0	37.0	37.0	35.0	37.0
8	36.273170658952296	37.0	37.0	37.0	35.0	37.0
9	38.18726453581102	39.0	39.0	39.0	37.0	39.0
10-11	38.08447060453069	39.0	38.0	39.0	37.0	39.0
12-13	38.124770627457835	39.0	38.0	39.0	37.0	39.0
14-15	39.55906952125524	41.0	39.0	41.0	37.0	41.0
16-17	39.54425943437838	41.0	39.0	41.0	37.0	41.0
18-19	39.595401923821555	41.0	39.5	41.0	37.0	41.0
20-21	39.61536584545034	41.0	40.0	41.0	37.0	41.0
22-23	39.55654741523858	41.0	39.0	41.0	37.0	41.0
24-25	39.516165019071295	41.0	39.0	41.0	37.0	41.0
26-27	39.479444771990416	41.0	39.0	41.0	37.0	41.0
28-29	39.388870531429	40.5	39.0	41.0	37.0	41.0
30-31	39.30199130767778	40.0	39.0	41.0	36.0	41.0
32-33	39.20479840219949	40.0	39.0	41.0	36.0	41.0
34-35	39.13472971820419	40.0	39.0	41.0	36.0	41.0
36-37	39.138258185726215	40.0	39.0	41.0	35.0	41.0
38-39	39.13723886250206	40.0	39.0	41.0	35.0	41.0
40-41	39.095597159359556	40.0	39.0	41.0	35.0	41.0
42-43	39.0612450271501	40.0	39.0	41.0	35.0	41.0
44-45	39.02933904388698	40.0	39.0	41.0	35.0	41.0
46-47	38.958608129026	40.0	38.5	41.0	35.0	41.0
48-49	38.93064998760469	40.0	38.5	41.0	35.0	41.0
50-51	38.84181037409376	40.0	38.0	41.0	35.0	41.0
52-53	38.67600300776445	40.0	38.0	41.0	35.0	41.0
54-55	38.54258878535132	40.0	38.0	41.0	34.5	41.0
56-57	38.38586164224104	40.0	37.5	41.0	34.0	41.0
58-59	38.197669803858474	40.0	37.0	41.0	34.0	41.0
60-61	38.06975950194537	40.0	37.0	41.0	34.0	41.0
62-63	37.89914350943458	39.0	36.0	41.0	34.0	41.0
64-65	37.64965727450734	39.0	36.0	41.0	34.0	41.0
66-67	37.37490302640907	39.0	35.0	40.0	33.0	41.0
68-69	37.118087347870556	38.0	35.0	40.0	33.0	41.0
70-71	36.822911748504524	37.0	35.0	40.0	33.0	41.0
72-73	36.47361955009101	37.0	35.0	39.0	33.0	41.0
74-75	36.095055638378106	36.0	35.0	39.0	32.5	40.5
76-77	34.16257620600133	34.5	33.0	36.5	29.5	39.0
78-79	35.20132437175005	35.0	34.0	37.0	31.5	39.0
80-81	35.12514224481623	35.0	34.0	37.0	32.0	39.0
82-83	34.852667410814874	35.0	34.0	36.5	32.0	39.0
84-85	34.60898723459648	35.0	34.0	36.0	31.5	37.5
86-87	34.40488727704728	35.0	34.0	36.0	31.0	37.0
88-89	34.25057143443206	35.0	34.0	35.5	31.0	37.0
90-91	34.1071900531024	35.0	34.0	35.0	31.0	36.0
92-93	34.022509778435726	35.0	34.0	35.0	31.0	36.0
94-95	33.94260647820009	35.0	34.0	35.0	31.0	36.0
96-97	33.90506728337787	35.0	34.0	35.0	31.0	36.0
98-99	33.92593287815485	35.0	34.0	35.0	31.0	35.5
100-101	32.68765184343109	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	8.0
20	35.0
21	92.0
22	408.0
23	1226.0
24	3336.0
25	7411.0
26	13930.0
27	23644.0
28	35864.0
29	51245.0
30	70311.0
31	95036.0
32	128387.0
33	178872.0
34	278086.0
35	456313.0
36	913218.0
37	2037699.0
38	2921941.0
39	1405417.0
40	26659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59171924511793	19.964958361751385	12.957486288519585	24.485836104611106
2	14.86807265990274	21.523734735002428	40.7103175965824	22.89787500851243
3	18.48334179702134	26.262754500323286	30.17171805466525	25.08218564799013
4	12.23943062058453	17.71426648858337	39.301544107989514	30.744758782842585
5	12.962423196112352	38.15118475954659	35.86610181660856	13.020290227732495
6	29.848346754515102	38.14588943477496	19.03597571966412	12.969788091045825
7	26.513113039344148	32.4824239730683	23.576265799405004	17.428197188182544
8	24.57674169727874	36.62539374075337	21.657579778316613	17.14028478365128
9	25.887963570938748	16.206395273212756	21.252447849263014	36.65319330658548
10-11	24.2120695915565	26.718438342450018	29.292889585251775	19.77660248074171
12-13	24.85715296552987	24.29952924647062	29.381692594569248	21.46162519343026
14-15	21.936122831299468	25.69369685810054	26.794616707736303	25.57556360286369
16-17	21.706453897708013	28.80856482236225	27.474105116819203	22.010876163110535
18-19	21.70153005981289	28.22872889428647	28.90603908666516	21.16370195923548
20-21	22.527185758073635	27.491591348193918	28.66992414002819	21.311298753704257
22-23	22.210754803355577	27.42678135601589	28.49908298550457	21.863380855123967
24-25	21.920926987530507	27.508787512638783	28.657066831357536	21.913218668473178
26-27	21.91003917451802	27.703601114763256	28.522030503622958	21.86432920709577
28-29	21.848704861404965	27.810449998709142	28.53626687916281	21.804578260723087
30-31	22.1151670456239	27.640108346395326	28.448490520850182	21.79623408713059
32-33	21.861006796579343	27.808262408985552	28.428200496036567	21.902530298398542
34-35	22.016578551185493	27.8195854065672	28.42584242141646	21.73799362083085
36-37	21.983204419344283	27.740674674514622	28.40827794692903	21.867842959212066
38-39	21.808421877007373	27.88949735257852	28.47462514417532	21.82745562623879
40-41	22.074627329160958	27.748913581267782	28.34129891816155	21.835160171409708
42-43	21.92482802869471	27.854475966307763	28.20627856874442	22.014417436253108
44-45	22.044310257313935	27.966959099868493	28.069940867942066	21.918789774875503
46-47	22.07467798264074	27.881532072292586	27.881414565201805	22.162375379864862
48-49	22.043002034087287	27.959724733586032	27.917855857738736	22.07941737458794
50-51	21.9930261591582	28.068774786354762	27.83649928929071	22.101699765196326
52-53	22.14432761962457	27.99106112667679	27.7020122715391	22.16259898215954
54-55	22.109020443810675	27.917004227422204	27.767592302086197	22.206383026680925
56-57	22.10596823224433	28.01630794684084	27.747848870482926	22.129874950431898
58-59	22.090417537524708	28.078070896164753	27.732350343163127	22.099161223147416
60-61	22.15696745344248	28.018116263486405	27.720765767692917	22.104150515378198
62-63	22.128590391876326	28.03261517566921	27.70151171676318	22.13728271569128
64-65	22.108034541101343	28.016422071914725	27.696211056306552	22.179332330677372
66-67	22.197100865209407	28.04003364316701	27.547332969106904	22.215532522516682
68-69	22.157393395403737	28.061462031058692	27.617874162093596	22.163270411443975
70-71	22.138480998452394	27.824395867395594	27.67952997543162	22.357593158720388
72-73	22.179752983436103	27.88691678773762	27.65891629371606	22.27441393511021
74-75	22.061915190548	28.013096906465545	27.632191940794275	22.292795962192173
76-77	22.201014421411617	27.940677984318697	27.515181964536982	22.3431256297327
78-79	22.14923556346298	28.010886755993585	27.485379809601152	22.354497870942282
80-81	22.181548004316227	27.958529953502172	27.50795023174335	22.351971810438258
82-83	22.22707017994803	27.925987628245025	27.584571748248642	22.262370443558304
84-85	22.308463147945208	27.912920802198975	27.533369737061726	22.245246312794087
86-87	22.147999424020178	27.931031561968457	27.617583280182913	22.303385733828453
88-89	22.21397256371002	27.92881091351971	27.568362992108607	22.28885353066167
90-91	22.23937204124886	27.913249127314554	27.608797066883966	22.23858176455262
92-93	22.269989814955558	27.91517212509972	27.57823572920872	22.236602330736005
94-95	22.139978749380358	28.004726170082616	27.618182408043836	22.23711267249319
96-97	22.164413744988877	28.016116386884686	27.636775890769794	22.182693977356646
98-99	22.41867017410636	28.579020547822065	27.69124882760482	21.31106045046676
100-101	26.43808911678034	35.63124051315748	28.31103931318847	9.619631056873711
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1427.0
1	1331.5
2	1905.0
3	2755.5
4	3812.0
5	6236.5
6	8295.0
7	9626.0
8	10328.0
9	9985.5
10	9009.0
11	8031.0
12	8634.0
13	11443.5
14	17605.0
15	25612.5
16	32014.0
17	35884.0
18	37215.0
19	36445.5
20	35169.0
21	34698.5
22	36194.5
23	41558.0
24	51334.0
25	65215.0
26	83393.0
27	103555.5
28	124219.5
29	145748.0
30	164599.5
31	182839.0
32	202443.0
33	220664.0
34	239347.0
35	257701.0
36	276953.5
37	294060.5
38	307491.5
39	320931.0
40	333563.5
41	344399.0
42	351421.0
43	357520.5
44	364637.0
45	370613.0
46	374233.0
47	376387.0
48	375202.0
49	369085.0
50	361827.0
51	351197.5
52	334883.5
53	319006.0
54	302493.5
55	281009.0
56	258571.5
57	235235.5
58	209445.0
59	182697.5
60	154964.5
61	127003.0
62	101542.5
63	79896.5
64	61187.0
65	46427.0
66	35132.5
67	26589.5
68	20134.5
69	14636.0
70	10480.0
71	7511.5
72	5340.5
73	3754.5
74	2655.0
75	1841.5
76	1216.5
77	802.5
78	557.0
79	392.0
80	276.5
81	189.0
82	127.0
83	80.5
84	57.5
85	53.0
86	46.0
87	35.5
88	22.0
89	10.0
90	6.0
91	4.5
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.54360578550073E-4
3	0.0049369075927673265
4	3.468553343864632E-5
5	0.0
6	0.0
7	0.0
8	5.434066905387923E-4
9	0.0025436057855007304
10-11	8.382337247672861E-4
12-13	3.1216980094781686E-4
14-15	9.249475583639018E-5
16-17	0.001537725315779987
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	6.146781904523544E-4
32-33	6.806131475146974E-4
34-35	2.3360924531949275E-5
36-37	7.460052008898021E-4
38-39	5.748017008204554E-4
40-41	3.5900315593691004E-5
42-43	1.0874688666725712E-4
44-45	9.039836237258565E-4
46-47	0.0
48-49	5.32202109574028E-4
50-51	1.900884823867814E-5
52-53	0.0
54-55	6.023593443972711E-4
56-57	7.863990192555697E-4
58-59	1.127788224192623E-4
60-61	1.4775825540484585E-4
62-63	7.475800663348181E-5
64-65	0.0
66-67	0.0
68-69	7.046781326254983E-6
70-71	4.4975902553924497E-4
72-73	0.0
74-75	7.3193239521070286E-6
76-77	0.0
78-79	2.853151448432366E-4
80-81	0.0
82-83	1.5428434512235442E-5
84-85	1.562838696448746E-5
86-87	2.849809573349467E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	8.404768966768971E-6
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	232.0
20-21	540.0
22-23	1125.0
24-25	2753.0
26-27	4604.0
28-29	11532.0
30-31	25812.0
32-33	31714.0
34-35	43070.0
36-37	69204.0
38-39	82507.0
40-41	79459.0
42-43	86440.0
44-45	100044.0
46-47	100076.0
48-49	96428.0
50-51	88397.0
52-53	83671.0
54-55	88922.0
56-57	92783.0
58-59	92356.0
60-61	89194.0
62-63	84323.0
64-65	85509.0
66-67	90562.0
68-69	91362.0
70-71	90560.0
72-73	84038.0
74-75	85745.0
76-77	84900.0
78-79	88710.0
80-81	89730.0
82-83	83803.0
84-85	81932.0
86-87	85005.0
88-89	90407.0
90-91	96116.0
92-93	92465.0
94-95	100488.0
96-97	153003.0
98-99	682413.0
100-101	5037205.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.49380774800038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.15148105415128	77.97544870495258
2	7.063654908194174	12.219248192551323
3	1.6635365627766585	4.316568349277209
4	0.5543809628537059	1.9180208168087909
5	0.24813820908854328	1.073120927591879
6	0.12415893545111467	0.644338745586306
7	0.061522968696600816	0.37249490785710254
8	0.04056150644982985	0.2806655312672688
9	0.023509038840048957	0.18300476571943217
>10	0.06783385230029454	0.8704818845371028
>50	8.858962792370471E-4	0.04919684436984704
>100	2.775628783639032E-4	0.046489563263277155
>500	1.9516191538675438E-5	0.00857519097211027
>1k	3.902584857861014E-5	0.04234557524572621
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1561844479548774E-5	0.0	0.0	0.0
3	0.0	1.1561844479548774E-5	0.0	0.0	0.0
4	0.0	2.3123688959097548E-5	0.0	2.3123688959097548E-5	0.0
5	0.0	3.468553343864632E-5	0.0	3.468553343864632E-5	0.0
6	6.937106687729264E-5	3.468553343864632E-5	0.0	4.6247377918195096E-5	0.0
7	6.937106687729264E-5	3.468553343864632E-5	0.0	5.7809222397743865E-5	0.0
8	6.937106687729264E-5	4.6247377918195096E-5	0.0	5.7809222397743865E-5	0.0
9	6.937106687729264E-5	1.271802892750365E-4	0.0	8.093291135684141E-5	0.0
10-11	6.937106687729264E-5	1.271802892750365E-4	0.0	1.271802892750365E-4	0.0
12-13	6.937106687729264E-5	1.271802892750365E-4	0.0	1.4452305599435967E-4	0.0
14-15	6.937106687729264E-5	1.734276671932316E-4	0.0	1.5608490047390846E-4	0.0
16-17	1.271802892750365E-4	1.8498951167278039E-4	0.0	1.9077043391255475E-4	0.0
18-19	1.271802892750365E-4	2.0233227839210353E-4	0.0	2.485796563102986E-4	0.0
20-21	1.271802892750365E-4	2.196750451114267E-4	0.0	3.237316454273657E-4	0.0
22-23	1.271802892750365E-4	2.4279873407052424E-4	0.0	3.7575994558533516E-4	0.0
24-25	1.271802892750365E-4	2.659224230296218E-4	0.0	4.0466455678420706E-4	0.0
26-27	1.271802892750365E-4	2.7748426750917056E-4	0.0	4.855974681410485E-4	0.0
28-29	1.271802892750365E-4	2.7748426750917056E-4	0.0	9.191666361241275E-4	0.0
30-31	1.271802892750365E-4	3.2373164542736564E-4	0.0	0.0026476623858166693	5.780922239774387E-6
32-33	1.271802892750365E-4	3.2373164542736564E-4	0.0	0.005647961028259576	1.1561844479548774E-5
34-35	1.271802892750365E-4	3.410744121466888E-4	0.0	0.009365094028434506	1.1561844479548774E-5
36-37	1.271802892750365E-4	3.641981011057864E-4	0.0	0.01527319655748393	1.734276671932316E-5
38-39	1.271802892750365E-4	3.6997902334556077E-4	0.0	0.024037074672981898	2.3123688959097548E-5
40-41	1.271802892750365E-4	4.2778824574330463E-4	0.0	0.03382995694715971	2.3123688959097548E-5
42-43	1.271802892750365E-4	4.451310124626278E-4	0.0	0.04247821661786219	2.3123688959097548E-5
44-45	1.271802892750365E-4	4.8559746814104843E-4	0.0	0.052484993014911656	2.3123688959097548E-5
46-47	1.271802892750365E-4	5.202830015796948E-4	0.0	0.06304673794697946	2.3123688959097548E-5
48-49	1.271802892750365E-4	5.202830015796948E-4	0.0	0.07402470928031102	2.3123688959097548E-5
50-51	1.329612115148109E-4	5.838731462172131E-4	0.0	0.08400258106616162	2.3123688959097548E-5
52-53	1.3874213375458528E-4	5.896540684569875E-4	0.0	0.09356422645074845	2.3123688959097548E-5
54-55	1.3874213375458528E-4	5.896540684569875E-4	0.0	0.1033282041137274	2.3123688959097548E-5
56-57	1.3874213375458528E-4	5.896540684569875E-4	0.0	0.11265283168648348	2.3123688959097548E-5
58-59	1.3874213375458528E-4	5.954349906967619E-4	0.0	0.1207056563664892	2.3123688959097548E-5
60-61	1.5030397823413406E-4	6.301205241354081E-4	0.0	0.12863708167945964	2.3123688959097548E-5
62-63	1.6186582271368282E-4	6.359014463751825E-4	0.0	0.13622165165804365	2.3123688959097548E-5
64-65	1.734276671932316E-4	7.052725132524752E-4	0.0	0.14340155707984342	2.3123688959097548E-5
66-67	1.734276671932316E-4	7.515198911706703E-4	0.0	0.15044850129012843	2.3123688959097548E-5
68-69	1.734276671932316E-4	7.977672690888653E-4	0.0	0.1577440251567237	2.3123688959097548E-5
70-71	1.734276671932316E-4	8.324528025275117E-4	0.0	0.16445567587710175	2.3123688959097548E-5
72-73	1.8498951167278039E-4	8.382337247672861E-4	0.0	0.17001692307176472	2.3123688959097548E-5
74-75	1.8498951167278039E-4	8.440146470070605E-4	0.0	0.17453760426326828	2.3123688959097548E-5
76-77	1.8498951167278039E-4	8.613574137263837E-4	0.0	0.1787808011872627	2.3123688959097548E-5
78-79	1.8498951167278039E-4	8.787001804457068E-4	0.0	0.18266558093239108	2.3123688959097548E-5
80-81	1.8498951167278039E-4	8.787001804457068E-4	0.0	0.18441141944880293	2.3123688959097548E-5
82-83	1.8498951167278039E-4	8.787001804457068E-4	0.0	0.1849374833726224	2.3123688959097548E-5
84-85	1.8498951167278039E-4	8.787001804457068E-4	0.0	0.1854751091409214	2.3123688959097548E-5
86-87	1.9655135615232914E-4	8.902620249252556E-4	0.0	0.18578727894186925	2.3123688959097548E-5
88-89	2.0811320063187792E-4	9.133857138843531E-4	0.0	0.18593758292010337	2.3123688959097548E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8785	0.0	44.398624	1
GTATCAA	14845	0.0	33.693577	1
TCAACGC	17445	0.0	27.746227	4
ATCAACG	17605	0.0	27.471172	3
CAACGCA	17925	0.0	26.933931	5
TATCAAC	18940	0.0	25.927767	2
AACGCAG	18675	0.0	25.896591	6
TACATGG	26175	0.0	21.877407	2
GTACATG	26925	0.0	21.837002	1
ACGCAGA	22270	0.0	21.75347	7
CGCAGAG	22520	0.0	21.475206	8
ACATGGG	26680	0.0	20.951656	3
CATGGGG	18920	0.0	19.105284	4
GCAGAGT	27680	0.0	17.561634	9
GAGTACT	14300	0.0	17.213678	12-13
GAGTACA	18880	0.0	16.755281	1
AGAGTAC	24435	0.0	16.394342	10-11
CAGAGTA	26615	0.0	16.2494	10-11
AGTACAT	19865	0.0	15.278327	2
GTGGTAT	5045	0.0	15.101394	1
>>END_MODULE