FastQCFastQC Report
Fri 27 May 2016
SRR2140005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9645753
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113900.042.841871
GTATCAA191650.032.7424281
TCAACGC221500.027.552694
ATCAACG222650.027.3545973
CAACGCA226000.026.985625
AACGCAG231800.026.364936
TATCAAC235100.026.119192
GTACATG309700.023.8039071
TACATGG306550.023.4316522
ACATGGG311950.022.6364823
ACGCAGA273050.022.3973437
CGCAGAG279650.021.8086228
CATGGGG236750.020.3453794
GAGTACT167300.018.17622212-13
GCAGAGT337800.017.967419
AGAGTAC288650.016.97073410-11
CAGAGTA325050.016.64780410-11
ATGGGGG151100.016.4397965
TATACCG8450.016.4146125
GAGTACA218650.016.377591
Produced by FastQC (version 0.11.5)