##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140005_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9645753
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75641372944134	34.0	31.0	34.0	31.0	34.0
2	32.9343941318008	34.0	31.0	34.0	31.0	34.0
3	33.02891490171892	34.0	33.0	34.0	31.0	34.0
4	36.4103296030906	37.0	37.0	37.0	35.0	37.0
5	36.258296889833275	37.0	37.0	37.0	35.0	37.0
6	36.315363248468	37.0	37.0	37.0	35.0	37.0
7	36.31210160575333	37.0	37.0	37.0	35.0	37.0
8	36.30107208841031	37.0	37.0	37.0	35.0	37.0
9	38.19057185063727	39.0	39.0	39.0	37.0	39.0
10-11	38.09265932892953	39.0	38.0	39.0	37.0	39.0
12-13	38.12478533298541	39.0	38.0	39.0	37.0	39.0
14-15	39.55933590669386	41.0	39.0	41.0	37.0	41.0
16-17	39.54151194831549	41.0	39.0	41.0	37.0	41.0
18-19	39.58739955294315	41.0	39.5	41.0	37.0	41.0
20-21	39.60015127523796	41.0	39.5	41.0	37.0	41.0
22-23	39.53894771415722	41.0	39.0	41.0	37.0	41.0
24-25	39.493682328368344	41.0	39.0	41.0	37.0	41.0
26-27	39.45188341988098	41.0	39.0	41.0	37.0	41.0
28-29	39.35752805191832	40.5	39.0	41.0	36.0	41.0
30-31	39.2617718004191	40.0	39.0	41.0	36.0	41.0
32-33	39.15643714351806	40.0	39.0	41.0	35.5	41.0
34-35	39.08298532755944	40.0	39.0	41.0	35.0	41.0
36-37	39.08226904254896	40.0	39.0	41.0	35.0	41.0
38-39	39.07275439063349	40.0	39.0	41.0	35.0	41.0
40-41	39.023151080464594	40.0	39.0	41.0	35.0	41.0
42-43	38.97943061417782	40.0	38.5	41.0	35.0	41.0
44-45	38.93798623090683	40.0	38.0	41.0	35.0	41.0
46-47	38.85745464823841	40.0	38.0	41.0	35.0	41.0
48-49	38.81866914070291	40.0	38.0	41.0	35.0	41.0
50-51	38.71493022938647	40.0	38.0	41.0	35.0	41.0
52-53	38.53537797185854	40.0	38.0	41.0	34.5	41.0
54-55	38.38372972532322	40.0	37.5	41.0	34.0	41.0
56-57	38.20956698400621	40.0	37.0	41.0	34.0	41.0
58-59	38.00545350835178	39.5	36.5	41.0	34.0	41.0
60-61	37.85851051281577	39.0	36.0	41.0	34.0	41.0
62-63	37.66715448359082	39.0	36.0	41.0	33.5	41.0
64-65	37.39487037402829	39.0	35.0	40.5	33.0	41.0
66-67	37.10959136970596	38.0	35.0	40.0	33.0	41.0
68-69	36.83845828257516	37.5	35.0	40.0	33.0	41.0
70-71	36.53464787072863	37.0	35.0	39.5	33.0	41.0
72-73	36.18435418394324	36.5	35.0	39.0	32.0	41.0
74-75	35.80630352676586	36.0	35.0	39.0	32.0	40.5
76-77	33.903922110195055	34.5	32.0	36.5	29.0	39.0
78-79	34.9857875467825	35.0	34.0	37.0	31.0	39.0
80-81	34.92587577708984	35.0	34.0	37.0	32.0	39.0
82-83	34.671432525461874	35.0	34.0	36.0	31.0	38.5
84-85	34.446302391490946	35.0	34.0	36.0	31.0	37.0
86-87	34.260831739179565	35.0	34.0	35.5	31.0	37.0
88-89	34.123768976880584	35.0	34.0	35.0	31.0	36.5
90-91	33.998607005296364	35.0	34.0	35.0	31.0	36.0
92-93	33.92504877874843	35.0	34.0	35.0	31.0	36.0
94-95	33.85453360290133	35.0	34.0	35.0	31.0	36.0
96-97	33.82207893168465	35.0	34.0	35.0	31.0	35.5
98-99	33.85223596888184	35.0	34.0	35.0	31.0	35.0
100-101	32.58804329618319	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	13.0
20	35.0
21	139.0
22	465.0
23	1601.0
24	4257.0
25	9279.0
26	17134.0
27	28785.0
28	43375.0
29	61950.0
30	84034.0
31	112181.0
32	152013.0
33	214790.0
34	337073.0
35	558040.0
36	1119726.0
37	2398002.0
38	3100520.0
39	1372164.0
40	30175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.963519799853884	19.60377795284619	13.104637864975393	24.328064382324534
2	15.153662748734313	21.035433902697296	40.4248655415959	23.386037806972492
3	18.674154801134314	25.889023300872633	29.93056517451394	25.506256723479115
4	12.729045151590581	17.266162064519396	38.33837303077801	31.666419753112017
5	13.29982496956432	37.92754965372836	35.43774870406669	13.334876672640627
6	30.766970707211765	37.345907571964574	18.48135650995832	13.405765210865342
7	27.04634879205387	32.29404692407114	23.012075884588793	17.647528399286195
8	25.158645722770316	36.12214595800452	21.078311945232418	17.64089637399274
9	26.133663601402436	16.219141821396885	20.836062585882033	36.811131991318646
10-11	24.768743312411676	26.295662083296058	28.65012341811095	20.28547118618132
12-13	25.369624923955943	23.88197609635109	28.839037899073205	21.909361080619767
14-15	22.429525617477314	25.206041755846805	26.265561404885617	26.098871221790265
16-17	22.215201172597073	28.40683387385624	26.880992759598733	22.496972193947954
18-19	22.30831537983608	27.72848319877152	28.384657994041522	21.578543427350876
20-21	23.005872996166314	26.942866910633988	28.188168111955754	21.863091981243944
22-23	22.79496107228541	26.82506225458598	28.074565736871687	22.305410936256923
24-25	22.537590695371147	26.944191153498636	28.16599926627007	22.352218884860147
26-27	22.475407264497672	27.151995337189543	28.05307017807778	22.319527220235003
28-29	22.39175395580774	27.3039910735957	28.026489569839587	22.277765400756973
30-31	22.661665577337462	27.114321448234456	27.930406029461846	22.293606944966236
32-33	22.433818253936607	27.289336307436585	27.894034150065032	22.38281128856177
34-35	22.53386205597542	27.304032217833285	27.927464694680136	22.234641031511156
36-37	22.56581398882614	27.211653509018046	27.93645909716736	22.286073404988453
38-39	22.415272449325506	27.314022948214696	27.972022199100365	22.298682403359436
40-41	22.595257164589576	27.224237381729598	27.84745879444411	22.333046659236718
42-43	22.522341387765078	27.33867577362219	27.6645235372028	22.474459301409937
44-45	22.567230045143475	27.408416653118955	27.55699622846905	22.467357073268523
46-47	22.64552064778608	27.368367948326078	27.35132285309752	22.634788550790322
48-49	22.572991630442125	27.500139970760678	27.32162106838225	22.605247330414947
50-51	22.535871691177626	27.603901541160784	27.232368906252592	22.627857861408998
52-53	22.744826229384977	27.443219295925044	27.145489980734727	22.666464493955253
54-55	22.664064403164478	27.354075973118757	27.23542779773089	22.746431825985876
56-57	22.672089251631427	27.507305137207936	27.135242427666455	22.685363183494182
58-59	22.649303331272726	27.530087348559412	27.15679692066913	22.66381239949873
60-61	22.699061269037387	27.499055085645292	27.17802021237592	22.62386343294141
62-63	22.68604694499728	27.537842975021803	27.111107135669403	22.665002944311517
64-65	22.68319179780846	27.516223260992955	27.08910411447205	22.711480826726536
66-67	22.759192432671757	27.483612865842062	26.980080316176867	22.77711438530931
68-69	22.66779467704745	27.531658422399786	27.07552174126608	22.72502515928668
70-71	22.70066669290517	27.33192664188729	27.083480041283853	22.88392662392369
72-73	22.761635689872133	27.425631267819583	27.0060209946513	22.806712047656983
74-75	22.593541530668798	27.507792175127932	27.088911294003466	22.809755000199807
76-77	22.73725787269933	27.428125079717507	27.000803713939835	22.833813333643324
78-79	22.69168166099193	27.459899970437284	26.956046196269373	22.892372172301414
80-81	22.711492774284842	27.505433585276574	26.98654207904013	22.796531561398453
82-83	22.745023357271197	27.430740401859733	27.059183264975356	22.765052975893713
84-85	22.805065274452758	27.443381909433455	26.97033602740437	22.781216788709408
86-87	22.642218607039105	27.468694285355078	27.103301417393283	22.785785690212535
88-89	22.702509035966045	27.419543457436806	27.029722134597705	22.848225371999437
90-91	22.7480904270952	27.444900259702337	27.02743039265713	22.77957892054533
92-93	22.790584064473528	27.447339434009432	26.989247118989006	22.77282938252803
94-95	22.629341853763137	27.42230215507655	27.104541620540118	22.843814370620194
96-97	22.669894405563237	27.447441398658505	27.142441728309713	22.740222467468545
98-99	22.92858695884396	28.100905937706344	27.101396548877048	21.869110554572647
100-101	27.230019204904742	35.10959954268989	27.647513656260102	10.012867596145261
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1847.0
1	1656.5
2	2317.5
3	3401.0
4	4671.5
5	7663.5
6	10183.0
7	11791.5
8	12752.5
9	12328.5
10	11072.5
11	9848.5
12	10346.5
13	13298.0
14	20517.0
15	30077.5
16	37674.5
17	42355.5
18	43459.0
19	41432.5
20	38263.5
21	35639.0
22	35229.5
23	38985.0
24	47167.0
25	59603.0
26	75798.0
27	93511.5
28	113377.5
29	134824.5
30	154130.5
31	173247.0
32	193668.5
33	213379.0
34	232092.0
35	250708.0
36	271843.0
37	291456.0
38	308201.5
39	323286.0
40	337689.0
41	351788.5
42	363045.0
43	375591.0
44	388505.5
45	400922.5
46	411510.0
47	418774.5
48	420551.5
49	417179.5
50	413745.5
51	407185.5
52	395599.5
53	380573.5
54	362695.0
55	339890.5
56	314279.0
57	287230.5
58	258277.0
59	226912.5
60	193362.5
61	161079.0
62	130469.0
63	102755.0
64	79158.0
65	60817.5
66	46921.0
67	36781.5
68	28747.0
69	21208.5
70	15491.5
71	11362.5
72	8194.5
73	5828.5
74	4153.0
75	2901.0
76	1985.0
77	1288.5
78	841.0
79	593.0
80	419.0
81	287.5
82	196.5
83	141.5
84	100.0
85	68.5
86	51.5
87	40.5
88	29.0
89	18.5
90	14.5
91	12.5
92	12.5
93	10.0
94	4.5
95	1.5
96	1.0
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.969778823903121E-4
3	0.004654898378592112
4	2.0734513935822324E-5
5	2.0734513935822324E-5
6	0.0
7	0.0
8	4.8726107749182465E-4
9	0.0026436505268173463
10-11	8.915840992403599E-4
12-13	3.0065045206942374E-4
14-15	1.4514159755075627E-4
16-17	0.00160692483002623
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	7.537654221704971E-4
32-33	7.453445415083417E-4
34-35	5.75213536259605E-5
36-37	8.721949605836183E-4
38-39	6.614769128417382E-4
40-41	2.667396750940044E-5
42-43	1.0219711424394872E-4
44-45	9.990998327702356E-4
46-47	0.0
48-49	4.882999719948868E-4
50-51	3.924323128542157E-5
52-53	5.6599300104374765E-6
54-55	6.800094812750532E-4
56-57	9.064760380276513E-4
58-59	1.0505348097627367E-4
60-61	1.3570403163107574E-4
62-63	1.788948448945916E-5
64-65	6.025351909171917E-6
66-67	6.091757833376168E-6
68-69	2.465236619882022E-5
70-71	4.428507362237557E-4
72-73	0.0
74-75	1.9150879373270875E-5
76-77	0.0
78-79	2.8758694357890576E-4
80-81	0.0
82-83	6.7055966787447866E-6
84-85	1.3577274412397628E-5
86-87	3.0244817361514995E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	7.284576497304634E-6
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	225.0
20-21	602.0
22-23	1286.0
24-25	3771.0
26-27	5189.0
28-29	10671.0
30-31	24347.0
32-33	29232.0
34-35	39896.0
36-37	62628.0
38-39	76695.0
40-41	75622.0
42-43	83691.0
44-45	98284.0
46-47	98823.0
48-49	94223.0
50-51	85981.0
52-53	82833.0
54-55	88869.0
56-57	92496.0
58-59	92908.0
60-61	90789.0
62-63	86697.0
64-65	88827.0
66-67	94311.0
68-69	96398.0
70-71	95703.0
72-73	89995.0
74-75	90497.0
76-77	90492.0
78-79	96145.0
80-81	97721.0
82-83	92023.0
84-85	90927.0
86-87	93645.0
88-89	100348.0
90-91	107092.0
92-93	104823.0
94-95	114435.0
96-97	174771.0
98-99	793633.0
100-101	5808209.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.74607862430618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.93590179170634	74.48033023971811
2	7.562060322260611	12.665857966195665
3	1.9401830454129816	4.87448165600104
4	0.7151223692524464	2.3955477664566196
5	0.32631109288449894	1.366363722034426
6	0.17960650942488435	0.9024804515840132
7	0.09819378585511325	0.5756341157448419
8	0.06461994551618637	0.432933363031754
9	0.03844506115436484	0.2897660802734728
>10	0.13593953810150358	1.7441541239072367
>50	0.0028727381046061338	0.15104425087026072
>100	7.224911212413232E-4	0.10118717247169662
>500	0.0	0.0
>1k	2.130920521298682E-5	0.020219091710883094
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.110177090373349E-5	0.0	0.0	0.0
3	0.0	3.110177090373349E-5	0.0	2.0734513935822324E-5	0.0
4	0.0	1.1403982664702279E-4	0.0	6.220354180746697E-5	1.0367256967911162E-5
5	0.0	1.1403982664702279E-4	0.0	6.220354180746697E-5	1.0367256967911162E-5
6	0.0	1.1403982664702279E-4	0.0	7.257079877537814E-5	2.0734513935822324E-5
7	0.0	1.1403982664702279E-4	0.0	8.29380557432893E-5	2.0734513935822324E-5
8	0.0	1.3477434058284512E-4	0.0	8.29380557432893E-5	2.0734513935822324E-5
9	0.0	3.421194799410684E-4	0.0	1.2440708361493395E-4	2.0734513935822324E-5
10-11	0.0	3.421194799410684E-4	0.0	2.3844691026195675E-4	2.0734513935822324E-5
12-13	0.0	3.421194799410684E-4	0.0	3.2656859448920164E-4	2.5918142419777907E-5
14-15	0.0	4.8726107749182465E-4	0.0	3.369358514571128E-4	4.665265635560023E-5
16-17	0.0	4.8726107749182465E-4	0.0	3.991393932645797E-4	8.29380557432893E-5
18-19	0.0	5.131792199116026E-4	0.0	4.6134293507204676E-4	8.29380557432893E-5
20-21	0.0	5.546482477832472E-4	0.0	5.390973623313805E-4	8.812168422724488E-5
22-23	0.0	5.857500186869807E-4	0.0	6.06484532622803E-4	1.1922345513097838E-4
24-25	0.0	6.686880744302701E-4	0.0	6.531371889784033E-4	1.3477434058284512E-4
26-27	0.0	7.153407307858703E-4	0.0	7.257079877537815E-4	1.3477434058284512E-4
28-29	0.0	7.153407307858703E-4	0.0	0.0010056239258873828	1.3477434058284512E-4
30-31	0.0	7.67177015625426E-4	0.0	0.0020216151087426764	1.399579690668007E-4
32-33	0.0	7.67177015625426E-4	0.0	0.003918823133870419	1.5550885451866744E-4
34-35	1.0367256967911162E-5	8.967677277243156E-4	0.0	0.006132232496519453	1.5550885451866744E-4
36-37	1.0367256967911162E-5	0.0010263584398232052	0.0	0.00976077243528836	1.5550885451866744E-4
38-39	1.0367256967911162E-5	0.0010678274676948498	0.0	0.015893004931807814	1.5550885451866744E-4
40-41	1.0367256967911162E-5	0.001150765523438139	0.0	0.022579885676110512	1.658761114865786E-4
42-43	1.0367256967911162E-5	0.0011715000373739614	0.0	0.028535874804175475	1.658761114865786E-4
44-45	1.0367256967911162E-5	0.0013373761488605401	0.0	0.03502059403760391	1.658761114865786E-4
46-47	1.0367256967911162E-5	0.0014825177464112963	0.0	0.04186298363642527	1.7105973997053416E-4
48-49	1.0367256967911162E-5	0.0014825177464112963	0.0	0.049203001569706376	1.9697788239031208E-4
50-51	1.0367256967911162E-5	0.0017002301427374307	0.0	0.056149063738206856	1.9697788239031208E-4
52-53	1.5550885451866744E-5	0.001731331913641164	0.0	0.06297590245157636	2.1252876784217883E-4
54-55	2.0734513935822324E-5	0.0017468827990930308	0.0	0.06984939382130145	2.3326328177800115E-4
56-57	2.0734513935822324E-5	0.0018039027124165422	0.0	0.07636003119714967	2.436305387459123E-4
58-59	2.0734513935822324E-5	0.00182982085483632	0.0	0.08255965086396054	2.5399779571382345E-4
60-61	2.0734513935822324E-5	0.0018661062542240092	0.0	0.0893813059488461	2.5918142419777905E-4
62-63	2.0734513935822324E-5	0.0018764735111919204	0.0	0.09591786146711408	2.6954868116569025E-4
64-65	2.0734513935822324E-5	0.0020941859075180548	0.0	0.10206046122060144	2.6954868116569025E-4
66-67	2.0734513935822324E-5	0.002104553164485966	0.0	0.10853999682554591	2.6954868116569025E-4
68-69	2.0734513935822324E-5	0.0021512058208415664	0.0	0.11457892400935417	2.6954868116569025E-4
70-71	2.0734513935822324E-5	0.0021823075917452995	0.0	0.12066968747800197	2.799159381336014E-4
72-73	2.0734513935822324E-5	0.0021926748487132107	0.0	0.1258584995904415	3.2138496600524603E-4
74-75	2.0734513935822324E-5	0.0022341438765848556	0.0	0.13028013468725563	3.2138496600524603E-4
76-77	2.0734513935822324E-5	0.0023326328177800115	0.0	0.1341937741926421	3.2138496600524603E-4
78-79	2.0734513935822324E-5	0.0023844691026195675	0.0	0.1380089247568334	3.2656859448920164E-4
80-81	2.0734513935822324E-5	0.002389652731103523	0.0	0.13960029870140778	3.4730310842502393E-4
82-83	2.0734513935822324E-5	0.0024000199880714343	0.0	0.1401342124352552	3.9395576478062417E-4
84-85	2.0734513935822324E-5	0.002436305387459123	0.0	0.14056963722790744	3.9395576478062417E-4
86-87	2.0734513935822324E-5	0.002436305387459123	0.0	0.14077179873878173	3.991393932645797E-4
88-89	2.0734513935822324E-5	0.002514059814718457	0.0	0.14087028767997686	4.043230217485353E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11390	0.0	42.84187	1
GTATCAA	19165	0.0	32.742428	1
TCAACGC	22150	0.0	27.55269	4
ATCAACG	22265	0.0	27.354597	3
CAACGCA	22600	0.0	26.98562	5
AACGCAG	23180	0.0	26.36493	6
TATCAAC	23510	0.0	26.11919	2
GTACATG	30970	0.0	23.803907	1
TACATGG	30655	0.0	23.431652	2
ACATGGG	31195	0.0	22.636482	3
ACGCAGA	27305	0.0	22.397343	7
CGCAGAG	27965	0.0	21.808622	8
CATGGGG	23675	0.0	20.345379	4
GAGTACT	16730	0.0	18.176222	12-13
GCAGAGT	33780	0.0	17.96741	9
AGAGTAC	28865	0.0	16.970734	10-11
CAGAGTA	32505	0.0	16.647804	10-11
ATGGGGG	15110	0.0	16.439796	5
TATACCG	845	0.0	16.414612	5
GAGTACA	21865	0.0	16.37759	1
>>END_MODULE