FastQCFastQC Report
Fri 27 May 2016
SRR2140004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8345666
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112250.037.891341
GTATCAA183550.031.5145261
TCAACGC206400.027.2625244
CAACGCA207600.027.063965
ATCAACG208450.026.9553783
AACGCAG214800.026.2361476
TATCAAC226400.025.2494372
GTACATG256250.024.4990231
TACATGG263250.023.2983822
ACATGGG264650.022.6295153
ACGCAGA251100.022.307847
CGCAGAG255300.021.940858
CATGGGG209200.019.9851634
GAGTACT155150.018.22968712-13
GCAGAGT306600.018.214139
AGAGTAC255800.017.7822110-11
GAGTACA184350.017.4654181
CAGAGTA289050.016.62688610-11
ATGGGGG133100.016.488745
AGTACAT192700.015.9141132
Produced by FastQC (version 0.11.5)