##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140004_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8345666
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.757835983371486	34.0	31.0	34.0	31.0	34.0
2	32.93120489125733	34.0	31.0	34.0	31.0	34.0
3	33.023978314013526	34.0	33.0	34.0	31.0	34.0
4	36.40678562981073	37.0	37.0	37.0	35.0	37.0
5	36.254024783642194	37.0	37.0	37.0	35.0	37.0
6	36.31415839071441	37.0	37.0	37.0	35.0	37.0
7	36.307448081435325	37.0	37.0	37.0	35.0	37.0
8	36.29626622968137	37.0	37.0	37.0	35.0	37.0
9	38.1806153037996	39.0	39.0	39.0	37.0	39.0
10-11	38.083348051551546	39.0	38.0	39.0	37.0	39.0
12-13	38.116465600228906	39.0	38.0	39.0	37.0	39.0
14-15	39.54924004866717	41.0	39.0	41.0	37.0	41.0
16-17	39.53099728649577	41.0	39.0	41.0	37.0	41.0
18-19	39.57536330833273	41.0	39.5	41.0	37.0	41.0
20-21	39.588596740740265	41.0	39.5	41.0	37.0	41.0
22-23	39.52621286270828	41.0	39.0	41.0	37.0	41.0
24-25	39.478885689846074	41.0	39.0	41.0	37.0	41.0
26-27	39.43413655056645	41.0	39.0	41.0	37.0	41.0
28-29	39.33848415423527	40.0	39.0	41.0	36.0	41.0
30-31	39.24379968574037	40.0	39.0	41.0	36.0	41.0
32-33	39.135978699607165	40.0	39.0	41.0	35.5	41.0
34-35	39.0586028442326	40.0	39.0	41.0	35.0	41.0
36-37	39.05424243109883	40.0	39.0	41.0	35.0	41.0
38-39	39.04424129496879	40.0	39.0	41.0	35.0	41.0
40-41	38.98899920469156	40.0	38.5	41.0	35.0	41.0
42-43	38.94011402047916	40.0	38.0	41.0	35.0	41.0
44-45	38.89352697840624	40.0	38.0	41.0	35.0	41.0
46-47	38.804475318218834	40.0	38.0	41.0	35.0	41.0
48-49	38.75972972136226	40.0	38.0	41.0	35.0	41.0
50-51	38.64729992434005	40.0	38.0	41.0	35.0	41.0
52-53	38.45823116175026	40.0	38.0	41.0	34.5	41.0
54-55	38.299492790042926	40.0	37.0	41.0	34.0	41.0
56-57	38.1118539599696	40.0	37.0	41.0	34.0	41.0
58-59	37.893227199528674	39.5	36.5	41.0	34.0	41.0
60-61	37.73300550399799	39.0	36.0	41.0	33.5	41.0
62-63	37.531959633183504	39.0	35.5	41.0	33.0	41.0
64-65	37.24790825378578	38.5	35.0	40.0	33.0	41.0
66-67	36.954379084292796	38.0	35.0	40.0	33.0	41.0
68-69	36.67969838099329	37.0	35.0	40.0	33.0	41.0
70-71	36.37122687758577	37.0	35.0	39.0	32.5	41.0
72-73	36.02229443496607	36.0	35.0	39.0	32.0	41.0
74-75	35.651307585679234	36.0	35.0	38.5	32.0	40.0
76-77	33.770803159877374	34.5	32.0	36.5	29.0	39.0
78-79	34.85878753201405	35.0	34.0	37.0	31.0	39.0
80-81	34.811441539269765	35.0	34.0	36.5	31.0	39.0
82-83	34.571327933040784	35.0	34.0	36.0	31.0	38.0
84-85	34.36021261298366	35.0	34.0	36.0	31.0	37.0
86-87	34.18659369918781	35.0	34.0	35.5	31.0	37.0
88-89	34.05848090994053	35.0	34.0	35.0	31.0	36.0
90-91	33.94156483794989	35.0	34.0	35.0	31.0	36.0
92-93	33.87209211006483	35.0	34.0	35.0	31.0	36.0
94-95	33.803930937965305	35.0	34.0	35.0	31.0	36.0
96-97	33.77511306914102	35.0	34.0	35.0	31.0	35.0
98-99	33.80850782008352	35.0	34.0	35.0	31.0	35.0
100-101	32.505867900375065	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	2.0
19	6.0
20	32.0
21	106.0
22	422.0
23	1552.0
24	3916.0
25	8649.0
26	16259.0
27	26531.0
28	40008.0
29	56607.0
30	76283.0
31	102214.0
32	136980.0
33	193491.0
34	305579.0
35	511552.0
36	1035303.0
37	2161578.0
38	2576624.0
39	1068547.0
40	23424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32254609757927	19.3059607226074	12.92077828180519	24.450714898008137
2	15.510644100355922	20.79844891761654	39.9138066558219	23.777100326205634
3	18.936700081470978	25.4738503802678	29.672571505192803	25.916878033068425
4	13.010181095237	16.95836304936312	37.73207260680516	32.29938324859472
5	13.649781053996296	37.606961218788435	35.06719955809393	13.676058169121333
6	31.44515967928743	36.748019870433346	18.13697073427094	13.669849716008283
7	27.60992352198135	31.937391215991628	22.638780416086625	17.813904845940396
8	25.56098527744284	35.854457465099394	20.68317438985126	17.901382867606504
9	26.266152533599186	16.092321075940873	20.57625481700186	37.065271573458084
10-11	25.177085988801874	26.036497708221436	28.2665191237171	20.51989717925959
12-13	25.71523625407009	23.587974262949945	28.535381494084213	22.16140798889575
14-15	22.798150126778577	24.841249690108132	25.896313925345325	26.464286257767967
16-17	22.58811697821379	28.027222638545023	26.545697265873542	22.83896311736764
18-19	22.658191688955682	27.370511832129395	28.047845432587405	21.923451046327518
20-21	23.332080335795354	26.544688934074557	27.91479201339289	22.2084387167372
22-23	23.17515696140974	26.423198471532434	27.804582310861665	22.597062256196157
24-25	22.90783471671915	26.629785434558308	27.813917986444864	22.648461862277678
26-27	22.849438561695383	26.691990389064006	27.852805219071342	22.60576583016927
28-29	22.776500382218853	26.775859941430504	27.826114447056828	22.62152522929382
30-31	22.98452827792842	26.69994839735991	27.661700035192627	22.653823289519043
32-33	22.779906998158047	26.877708104052356	27.662557879337434	22.67982701845216
34-35	22.95152071142259	26.81819139445195	27.659768282031244	22.570519612094216
36-37	22.948354934010016	26.74790742655454	27.619381435525902	22.684356203909548
38-39	22.805483345054625	26.86694445741842	27.69125593264633	22.636316264880623
40-41	22.95638009878648	26.7527614228735	27.596186859805787	22.69467161853423
42-43	22.84043352490026	26.87924483652685	27.424464427167994	22.8558572114049
44-45	22.90966767539195	26.92498062009585	27.335971090424948	22.829380614087253
46-47	23.059121655745948	26.94254922441267	27.05499851508786	22.943330604753516
48-49	22.9215094108036	27.085653652212354	27.005401934236666	22.987435002747375
50-51	22.884942025737505	27.174066896180708	26.99600803521381	22.944983042867978
52-53	23.1228762376372	27.04001745876008	26.8300766445256	23.007029659077112
54-55	23.002221224465956	26.939653823770517	26.931707842364244	23.12641710939928
56-57	23.031514721997276	27.06312502109044	26.924646767642475	22.98071348926981
58-59	22.997739838120797	27.110865213715897	26.862568171610523	23.028826776552787
60-61	23.06292466868164	27.057697399429525	26.888399974354567	22.990977957534273
62-63	23.057740220564675	27.126684301061903	26.860110146249838	22.955465332123584
64-65	23.027058263787488	27.11511554116049	26.81832328125442	23.039502913797598
66-67	23.088185147808176	27.059795596333817	26.732405974451613	23.11961328140639
68-69	22.99609234588739	27.07968438478867	26.834674583559387	23.08954868576455
70-71	23.0659115742089	26.920380893039763	26.848774378667102	23.16493315408424
72-73	23.106015243902654	26.953238703430195	26.750580574562505	23.19016547810464
74-75	22.917061041854062	27.11633679960589	26.823815537226594	23.142786621313462
76-77	23.135074757329168	26.992186102328557	26.728912397875593	23.14382674246668
78-79	23.04979663045304	26.975434326586235	26.720545280521296	23.25422376243943
80-81	23.05360861821829	27.04176555712683	26.76112910671547	23.143496717939406
82-83	23.126171194909485	26.95855498896944	26.80197538395563	23.113298432165447
84-85	23.110159256178083	26.957937331887983	26.74111913999983	23.190784271934106
86-87	23.060059675128194	26.97189312904155	26.87198359884905	23.096063596981207
88-89	23.052279948397736	26.87282566255771	26.8248224625532	23.250071926491362
90-91	23.116200143095273	26.879936173377654	26.815242306961355	23.188621376565717
92-93	23.178493930906146	26.856822525014547	26.84360203021657	23.121081513862737
94-95	23.04772976853455	26.79472502454009	26.96135127191451	23.196193935010854
96-97	23.100850817567867	26.815198373500444	26.91459353292703	23.16935727600466
98-99	23.35698425544451	27.410266459512556	26.936341674744373	22.29640761029857
100-101	24.218986258107993	29.86362731991227	23.903644823859914	22.013741598119825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1094.0
1	1022.0
2	1703.0
3	2652.5
4	3640.5
5	5994.5
6	8059.0
7	9585.5
8	10759.5
9	10608.0
10	9388.5
11	8121.0
12	8552.0
13	11141.0
14	17184.0
15	25203.5
16	31661.5
17	35605.0
18	36218.5
19	34293.5
20	31440.5
21	28587.0
22	27715.5
23	30119.0
24	35888.5
25	45432.5
26	57282.0
27	69781.5
28	84567.0
29	102213.0
30	119127.5
31	135880.0
32	153602.5
33	170498.5
34	185168.0
35	198768.0
36	215562.5
37	232249.5
38	247118.5
39	259862.5
40	270341.5
41	281312.5
42	291067.0
43	301668.0
44	313625.5
45	327060.0
46	338329.5
47	345928.5
48	353197.5
49	354505.0
50	353381.5
51	353058.5
52	347292.0
53	339539.5
54	330823.0
55	315974.5
56	298204.5
57	276260.5
58	250740.5
59	221802.0
60	189076.5
61	157633.0
62	127789.0
63	101010.5
64	77781.5
65	59256.5
66	45632.5
67	35366.5
68	26912.0
69	19633.5
70	14246.5
71	10552.5
72	7690.0
73	5411.0
74	3882.5
75	2756.0
76	1902.5
77	1206.0
78	756.0
79	503.5
80	334.0
81	238.0
82	177.5
83	120.0
84	76.5
85	55.5
86	46.5
87	37.5
88	26.0
89	16.5
90	9.5
91	5.5
92	3.0
93	3.0
94	2.5
95	1.5
96	1.0
97	1.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.1982267203120757E-4
3	0.004349562994732835
4	2.3964534406241515E-5
5	1.1982267203120757E-5
6	0.0
7	0.0
8	4.4334388651546804E-4
9	0.002707992387905291
10-11	8.806966394293757E-4
12-13	3.8343255049986424E-4
14-15	1.5576947364056984E-4
16-17	0.0013420139267495248
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	7.448119791234008E-4
32-33	7.103370657733596E-4
34-35	7.24393548285448E-5
36-37	8.182275397449481E-4
38-39	6.402259839191085E-4
40-41	3.6832668022804576E-5
42-43	1.1745478933339894E-4
44-45	9.972674249264873E-4
46-47	0.0
48-49	4.2565248714465957E-4
50-51	1.2821056893568175E-5
52-53	3.878573750174859E-5
54-55	8.147986733513184E-4
56-57	0.001019566333051407
58-59	1.1289838852824864E-4
60-61	1.5424793101549662E-4
62-63	2.7081917315792015E-5
64-65	0.0
66-67	0.0
68-69	2.790782046899092E-5
70-71	4.3739748496446145E-4
72-73	0.0
74-75	7.2086853124118064E-6
76-77	0.0
78-79	2.7268668462075105E-4
80-81	0.0
82-83	7.550006904481315E-6
84-85	3.055713136984411E-5
86-87	3.015135749529117E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	3.2739206763396294E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	204.0
20-21	643.0
22-23	1130.0
24-25	2971.0
26-27	4182.0
28-29	8300.0
30-31	17384.0
32-33	21525.0
34-35	29210.0
36-37	45881.0
38-39	55687.0
40-41	55598.0
42-43	62752.0
44-45	75864.0
46-47	75896.0
48-49	71805.0
50-51	66346.0
52-53	62773.0
54-55	68465.0
56-57	71913.0
58-59	73420.0
60-61	71682.0
62-63	68197.0
64-65	71378.0
66-67	76354.0
68-69	78873.0
70-71	78662.0
72-73	74129.0
74-75	74826.0
76-77	75169.0
78-79	80674.0
80-81	81608.0
82-83	77789.0
84-85	77269.0
86-87	80997.0
88-89	86133.0
90-91	94943.0
92-93	91679.0
94-95	99682.0
96-97	152753.0
98-99	710909.0
100-101	5170011.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.67802622692506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.55687203739792	73.21707388882433
2	7.656685097601207	12.660792226215575
3	2.0297003636395945	5.034348596933811
4	0.7600267398863688	2.513500429339582
5	0.37966383591567815	1.5694928291625703
6	0.20082204417826524	0.9962142141309188
7	0.11595767067082313	0.6711005935854817
8	0.07753734271996852	0.5128507563974107
9	0.05167551632838692	0.38451867248594407
>10	0.16715623232224774	2.1693331930403046
>50	0.00308362041273442	0.169336626945036
>100	7.929552722822942E-4	0.0890605588432927
>500	2.654365455358325E-5	0.012377414095795312
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1982267203120757E-5
2	0.0	1.1982267203120757E-5	0.0	0.0	1.1982267203120757E-5
3	0.0	1.1982267203120757E-5	0.0	0.0	1.1982267203120757E-5
4	0.0	4.792906881248303E-5	0.0	0.0	1.1982267203120757E-5
5	0.0	5.9911336015603785E-5	0.0	0.0	1.1982267203120757E-5
6	0.0	5.9911336015603785E-5	0.0	0.0	2.3964534406241515E-5
7	0.0	5.9911336015603785E-5	0.0	4.792906881248303E-5	2.3964534406241515E-5
8	0.0	5.9911336015603785E-5	0.0	4.792906881248303E-5	4.792906881248303E-5
9	0.0	1.9171627524993212E-4	0.0	4.792906881248303E-5	7.189360321872455E-5
10-11	0.0	1.9171627524993212E-4	0.0	5.392020241404341E-5	7.189360321872455E-5
12-13	0.0	1.9171627524993212E-4	0.0	9.585813762496606E-5	7.189360321872455E-5
14-15	0.0	2.6960101207021707E-4	0.0	1.2581380563276795E-4	1.1982267203120757E-4
16-17	0.0	2.7559214567177744E-4	0.0	1.7374287444525097E-4	1.5576947364056984E-4
18-19	0.0	2.9356554647645857E-4	0.0	2.4563647766397557E-4	1.5576947364056984E-4
20-21	0.0	3.295123480858208E-4	0.0	3.8343255049986424E-4	1.5576947364056984E-4
22-23	0.0	3.8343255049986424E-4	0.0	5.991133601560379E-4	2.2167194325773402E-4
24-25	5.991133601560379E-6	4.1937935210922654E-4	0.0	7.668651009997285E-4	2.9955668007801894E-4
26-27	2.3964534406241515E-5	4.4334388651546804E-4	0.0	8.926789066324964E-4	2.9955668007801894E-4
28-29	2.3964534406241515E-5	4.4334388651546804E-4	0.0	0.0020190120237258474	2.9955668007801894E-4
30-31	2.3964534406241515E-5	5.272197569373133E-4	0.0	0.0040440151810532555	3.115389472811397E-4
32-33	2.3964534406241515E-5	5.272197569373133E-4	0.0	0.007345129795513024	3.47485748890502E-4
34-35	2.3964534406241515E-5	6.290690281638398E-4	0.0	0.012275832749597215	3.714502832967435E-4
36-37	2.3964534406241515E-5	7.309182993903662E-4	0.0	0.018452691492805966	3.714502832967435E-4
38-39	2.3964534406241515E-5	7.608739673981681E-4	0.0	0.02751128549836526	4.1338821850766616E-4
40-41	2.3964534406241515E-5	8.028119026090907E-4	0.0	0.03603067747978412	4.313616193123473E-4
42-43	2.3964534406241515E-5	8.207853034137718E-4	0.0	0.04398091176905474	4.493350201170284E-4
44-45	2.3964534406241515E-5	8.866877730309361E-4	0.0	0.052434401280856434	4.792906881248303E-4
46-47	2.3964534406241515E-5	9.346168418434191E-4	0.0	0.06210409091377489	5.092463561326322E-4
48-49	2.9955668007801893E-5	9.346168418434191E-4	0.0	0.07034189961592041	5.751488257497964E-4
50-51	3.5946801609362274E-5	0.0010724129146793078	0.0	0.0785078147148472	5.751488257497964E-4
52-53	3.5946801609362274E-5	0.001090386315483989	0.0	0.08658985394335215	6.470424289685209E-4
54-55	3.5946801609362274E-5	0.0010963774490855495	0.0	0.09459400843503682	7.249271657888059E-4
56-57	4.193793521092265E-5	0.0011023685826871098	0.0	0.10228063284583878	7.369094329919266E-4
58-59	4.792906881248303E-5	0.00110835971628867	0.0	0.10932620596127379	7.488917001950474E-4
60-61	4.792906881248303E-5	0.001138315384296472	0.0	0.11671327369199774	7.668651009997285E-4
62-63	5.392020241404341E-5	0.0011502976514995928	0.0	0.12435796016758878	7.788473682028492E-4
64-65	5.9911336015603785E-5	0.001407916396366689	0.0	0.13182291263513302	7.788473682028492E-4
66-67	5.9911336015603785E-5	0.0014258897971713702	0.0	0.1395514749811459	7.788473682028492E-4
68-69	5.9911336015603785E-5	0.001467827732382293	0.0	0.1467468264366199	7.788473682028492E-4
70-71	5.9911336015603785E-5	0.0015097656675932155	0.0	0.15340297586795348	8.028119026090907E-4
72-73	5.9911336015603785E-5	0.0015097656675932155	0.0	0.15805209554276434	9.106523074371776E-4
74-75	5.9911336015603785E-5	0.001515756801194776	0.0	0.16230580039987222	9.106523074371776E-4
76-77	5.9911336015603785E-5	0.0015457124692025777	0.0	0.1656368706823398	9.226345746402983E-4
78-79	5.9911336015603785E-5	0.0015696770036088192	0.0	0.16906379910243233	9.346168418434191E-4
80-81	5.9911336015603785E-5	0.0015696770036088192	0.0	0.17053761796841618	9.465991090465398E-4
82-83	5.9911336015603785E-5	0.0015696770036088192	0.0	0.1711247490613691	0.001030474979468385
84-85	5.9911336015603785E-5	0.00158165927081194	0.0	0.17150818161186895	0.001030474979468385
86-87	5.9911336015603785E-5	0.0015936415380150607	0.0	0.17175381808953294	0.001048448380273066
88-89	5.9911336015603785E-5	0.0016535528740306644	0.0	0.1718976052959704	0.0010544395138746266
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11225	0.0	37.89134	1
GTATCAA	18355	0.0	31.514526	1
TCAACGC	20640	0.0	27.262524	4
CAACGCA	20760	0.0	27.06396	5
ATCAACG	20845	0.0	26.955378	3
AACGCAG	21480	0.0	26.236147	6
TATCAAC	22640	0.0	25.249437	2
GTACATG	25625	0.0	24.499023	1
TACATGG	26325	0.0	23.298382	2
ACATGGG	26465	0.0	22.629515	3
ACGCAGA	25110	0.0	22.30784	7
CGCAGAG	25530	0.0	21.94085	8
CATGGGG	20920	0.0	19.985163	4
GAGTACT	15515	0.0	18.229687	12-13
GCAGAGT	30660	0.0	18.21413	9
AGAGTAC	25580	0.0	17.78221	10-11
GAGTACA	18435	0.0	17.465418	1
CAGAGTA	28905	0.0	16.626886	10-11
ATGGGGG	13310	0.0	16.48874	5
AGTACAT	19270	0.0	15.914113	2
>>END_MODULE