FastQCFastQC Report
Fri 27 May 2016
SRR2140003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9066307
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109600.041.249391
GTATCAA186850.032.7112581
ATCAACG208150.028.5564123
TCAACGC210350.028.2772924
CAACGCA214400.027.7630755
AACGCAG219850.027.2664666
TATCAAC224300.026.7809312
GTACATG264050.023.609821
TACATGG264100.023.2232042
ACGCAGA257900.023.178347
CGCAGAG263100.022.7362378
ACATGGG271500.022.0793113
CATGGGG197800.020.3030014
GCAGAGT314700.018.92779
GAGTACT168450.018.37952212-13
AGAGTAC272050.017.21382910-11
CAGAGTA305750.016.89290210-11
ATGGGGG127050.016.3017185
GTACTTT194650.015.98162714-15
GAGTACA188150.015.7056791
Produced by FastQC (version 0.11.5)