##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140003_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9066307
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76277970732736	34.0	31.0	34.0	31.0	34.0
2	32.936758483911916	34.0	31.0	34.0	31.0	34.0
3	33.03173695750651	34.0	33.0	34.0	31.0	34.0
4	36.41566847449573	37.0	37.0	37.0	35.0	37.0
5	36.2648009823625	37.0	37.0	37.0	35.0	37.0
6	36.321147298453496	37.0	37.0	37.0	35.0	37.0
7	36.32011556634912	37.0	37.0	37.0	35.0	37.0
8	36.306943940901185	37.0	37.0	37.0	35.0	37.0
9	38.202500863912945	39.0	39.0	39.0	37.0	39.0
10-11	38.10437535371348	39.0	38.0	39.0	37.0	39.0
12-13	38.13984977565838	39.0	38.5	39.0	37.0	39.0
14-15	39.57928669302727	41.0	39.0	41.0	37.0	41.0
16-17	39.56562644525494	41.0	39.0	41.0	37.0	41.0
18-19	39.614617396035676	41.0	39.5	41.0	37.0	41.0
20-21	39.62964467526298	41.0	40.0	41.0	37.0	41.0
22-23	39.567238788095565	41.0	39.0	41.0	37.0	41.0
24-25	39.52223625333216	41.0	39.0	41.0	37.0	41.0
26-27	39.484763483388576	41.0	39.0	41.0	37.0	41.0
28-29	39.39091468248225	40.5	39.0	41.0	36.5	41.0
30-31	39.2979436511008	40.0	39.0	41.0	36.0	41.0
32-33	39.190940848378375	40.0	39.0	41.0	36.0	41.0
34-35	39.11508992917568	40.0	39.0	41.0	35.0	41.0
36-37	39.11777864313392	40.0	39.0	41.0	35.0	41.0
38-39	39.11096989185046	40.0	39.0	41.0	35.0	41.0
40-41	39.061096681104345	40.0	39.0	41.0	35.0	41.0
42-43	39.020847421467266	40.0	39.0	41.0	35.0	41.0
44-45	38.98201429902954	40.0	38.5	41.0	35.0	41.0
46-47	38.9052369718615	40.0	38.0	41.0	35.0	41.0
48-49	38.869108500515054	40.0	38.0	41.0	35.0	41.0
50-51	38.76780233434425	40.0	38.0	41.0	35.0	41.0
52-53	38.59029593055844	40.0	38.0	41.0	35.0	41.0
54-55	38.44781060824681	40.0	38.0	41.0	34.0	41.0
56-57	38.27707531512118	40.0	37.0	41.0	34.0	41.0
58-59	38.07717633050518	40.0	37.0	41.0	34.0	41.0
60-61	37.93601062750626	39.0	36.0	41.0	34.0	41.0
62-63	37.754968771897836	39.0	36.0	41.0	34.0	41.0
64-65	37.49684406617794	39.0	35.5	41.0	33.0	41.0
66-67	37.21672266848391	38.5	35.0	40.0	33.0	41.0
68-69	36.95872674139943	37.5	35.0	40.0	33.0	41.0
70-71	36.661901403154985	37.0	35.0	40.0	33.0	41.0
72-73	36.31736688549489	36.5	35.0	39.0	32.5	41.0
74-75	35.945395217487885	36.0	35.0	39.0	32.0	40.5
76-77	34.039058564453086	34.5	32.5	36.5	29.0	39.0
78-79	35.10282792333149	35.0	34.0	37.0	31.5	39.0
80-81	35.03591139235236	35.0	34.0	37.0	32.0	39.0
82-83	34.77054858407039	35.0	34.0	36.5	32.0	39.0
84-85	34.5384917570654	35.0	34.0	36.0	31.0	37.0
86-87	34.34113116547189	35.0	34.0	36.0	31.0	37.0
88-89	34.19415167155515	35.0	34.0	35.0	31.0	37.0
90-91	34.05958821608637	35.0	34.0	35.0	31.0	36.0
92-93	33.97973970057395	35.0	34.0	35.0	31.0	36.0
94-95	33.904283874583435	35.0	34.0	35.0	31.0	36.0
96-97	33.86791566661239	35.0	34.0	35.0	31.0	36.0
98-99	33.89489567287494	35.0	34.0	35.0	31.0	35.0
100-101	32.6537653408585	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	2.0
20	30.0
21	87.0
22	451.0
23	1517.0
24	3793.0
25	8619.0
26	15985.0
27	26455.0
28	40223.0
29	56767.0
30	77008.0
31	102501.0
32	137899.0
33	193294.0
34	303125.0
35	502152.0
36	1011790.0
37	2199631.0
38	2964236.0
39	1389307.0
40	31432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74096387867739	19.831922744288274	13.068000013677015	24.359113363357316
2	14.998816493905661	21.246956580437274	40.55467279751501	23.199554128142054
3	18.446199494810223	26.089555366814103	30.266029848112158	25.198215290263516
4	12.515778211618853	17.50395420403031	38.837673582387524	31.142594001963314
5	13.092028801596417	38.028301061634494	35.7045424138152	13.175127722953889
6	30.292543590240218	37.84144966633051	18.6482985850799	13.21770815834937
7	26.65445809412807	32.53237508943829	23.34315394349651	17.47001287293713
8	24.875436467331557	36.3075743385611	21.367836804096775	17.449152390010564
9	25.93569660358262	16.241224268157268	21.146512159150184	36.67656696910993
10-11	24.43405619559272	26.489084158161937	29.036533058349413	20.040326587895933
12-13	25.09918766514158	24.104611448132466	29.182673564833443	21.613527321892505
14-15	22.21483466285504	25.471540573418483	26.580770111885858	25.732854651840615
16-17	21.884995267018052	28.62721684116527	27.25469619682675	22.233091694989923
18-19	21.952014199386806	27.980786443697525	28.749384948027902	21.317814408887763
20-21	22.684291711653888	27.202717449662618	28.54193533300599	21.571055505677503
22-23	22.43788363814426	27.094350087633956	28.458943475946015	22.00882279827577
24-25	22.205782451968563	27.209491330338203	28.53541948501151	22.04930673268172
26-27	22.126536673121148	27.39789273129104	28.4474653278465	22.028105267741314
28-29	22.055297491611842	27.52066826292824	28.441735375881056	21.98229886957887
30-31	22.32432755860854	27.373979485808054	28.319604080806993	21.98208887477641
32-33	22.081524667028187	27.544782542605546	28.311066471071445	22.06262631929482
34-35	22.247147021309786	27.52598552579589	28.30625896573979	21.920608487154542
36-37	22.23394516478621	27.505224484386996	28.257708900881113	22.003121449945677
38-39	22.092748032950542	27.605430723702757	28.33096266684006	21.970858576506643
40-41	22.280859760413012	27.448046068866766	28.244449833000413	22.026644337719805
42-43	22.188241878184026	27.566140627560507	28.062464861501983	22.18315263275348
44-45	22.239609864049942	27.66456756429439	27.903802634612024	22.192019937043643
46-47	22.317476873982006	27.674005727746433	27.667702153923017	22.340815244348544
48-49	22.27604440786569	27.727735135268787	27.687596622666188	22.30862383419933
50-51	22.24216087625796	27.82222963240625	27.638244858273097	22.297364633062696
52-53	22.429018861993857	27.742621015489988	27.453463104942433	22.374897017573726
54-55	22.358633517936727	27.649452429111125	27.509233449192553	22.48268060375959
56-57	22.36661668851313	27.730727906952712	27.500620115918423	22.402035288615735
58-59	22.348591858548577	27.790969103762425	27.48486267549655	22.375576362192444
60-61	22.372452721143844	27.72809968987342	27.553570013150264	22.34587757583247
62-63	22.333595374206034	27.829459637664637	27.473260636699408	22.363684351429917
64-65	22.320141129654075	27.79235629273137	27.421415573660386	22.46608700395417
66-67	22.400060828406865	27.832984783554938	27.28352513505163	22.48342925298657
68-69	22.334550038097277	27.824737397416737	27.380343056756534	22.46036950772945
70-71	22.328584778402234	27.65612419717172	27.408102065113233	22.607188959312808
72-73	22.383201959504248	27.685637815626947	27.364971023179784	22.56618920168902
74-75	22.207704309482505	27.80613875302179	27.379138714242302	22.6070182232534
76-77	22.39782603954074	27.6900104359018	27.269449678653128	22.642713845904332
78-79	22.396155280080084	27.731251701955433	27.222281367168428	22.650311650796056
80-81	22.37147629199033	27.782407342150144	27.234573610108587	22.61154275575094
82-83	22.42374446172256	27.67737586222626	27.318829283570462	22.580050392480718
84-85	22.504215008878305	27.731430449277383	27.24307154430255	22.521282997541764
86-87	22.39244183334577	27.740096334148074	27.298919520579894	22.56854231192626
88-89	22.381798430949157	27.718112593414446	27.285972660643438	22.614116314992962
90-91	22.42745608742655	27.70630931895623	27.323953168421124	22.542281425196105
92-93	22.429577478197587	27.67330636933919	27.32691259728306	22.57020355518016
94-95	22.300397017164258	27.70569201328105	27.427739765825326	22.566171203729365
96-97	22.335230815503383	27.707536800440195	27.4371808533146	22.52005153074182
98-99	22.582851215939268	28.355528454798005	27.47130885373059	21.590311475532133
100-101	26.759509890744383	35.31109346687445	28.098617688678267	9.830778953702891
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1731.0
1	1526.5
2	2158.5
3	3238.0
4	4428.0
5	7177.0
6	9531.5
7	11105.5
8	12090.0
9	11788.0
10	10574.0
11	9255.0
12	9820.5
13	12921.0
14	20108.5
15	29556.5
16	37162.5
17	41909.5
18	43218.5
19	41600.5
20	38861.0
21	36737.5
22	37050.0
23	41462.0
24	49795.5
25	62140.0
26	78642.0
27	96797.0
28	117116.5
29	139759.5
30	161124.5
31	181990.5
32	202404.0
33	221214.0
34	238254.5
35	253689.5
36	271727.0
37	288316.5
38	301061.0
39	311997.5
40	323156.5
41	334421.5
42	342951.0
43	352652.5
44	364045.0
45	374066.0
46	380339.0
47	383872.5
48	383748.0
49	377986.5
50	371170.0
51	362967.5
52	350392.0
53	336911.5
54	321033.5
55	300987.0
56	279479.0
57	254743.0
58	227403.5
59	198919.5
60	168846.0
61	139502.0
62	112019.5
63	87358.0
64	66703.0
65	50901.5
66	39423.5
67	30830.5
68	23821.5
69	17512.5
70	12755.0
71	9650.0
72	7096.5
73	5084.5
74	3673.5
75	2572.0
76	1763.0
77	1146.0
78	772.0
79	547.5
80	377.0
81	258.0
82	190.0
83	134.0
84	93.0
85	70.5
86	58.0
87	43.5
88	31.0
89	25.0
90	18.5
91	12.0
92	9.0
93	6.0
94	2.5
95	1.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.757462327273939E-4
3	0.004489148668801972
4	7.720894516367028E-5
5	4.411939723638302E-5
6	0.0
7	0.0
8	5.514924654547878E-4
9	0.0028015817245103216
10-11	8.823879447276602E-4
12-13	3.6949995185470776E-4
14-15	1.5441789032734056E-4
16-17	0.0015552087525825013
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	7.133608336699677E-4
32-33	7.373017649174857E-4
34-35	1.1122451686433395E-5
36-37	8.93878019394248E-4
38-39	6.693469418128829E-4
40-41	1.7000582666636595E-5
42-43	9.138928448073437E-5
44-45	0.0010721452906738635
46-47	0.0
48-49	5.475009144148337E-4
50-51	1.7838349943116474E-5
52-53	3.600520563263037E-5
54-55	8.839669287386427E-4
56-57	0.0010088054655001371
58-59	4.323448016497784E-5
60-61	1.1234196294362904E-4
62-63	6.306639137766388E-6
64-65	7.646811126823891E-5
66-67	0.0
68-69	1.9560467174373804E-5
70-71	5.147577754491954E-4
72-73	0.0
74-75	1.351201623603871E-5
76-77	0.0
78-79	3.461005679648761E-4
80-81	0.0
82-83	2.842054390384307E-5
84-85	2.158682754513734E-5
86-87	3.571265886211452E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	2.3211038241346056E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	192.0
20-21	608.0
22-23	1246.0
24-25	3217.0
26-27	4806.0
28-29	9724.0
30-31	21077.0
32-33	26598.0
34-35	36103.0
36-37	56347.0
38-39	66464.0
40-41	68301.0
42-43	75568.0
44-45	90344.0
46-47	90870.0
48-49	85941.0
50-51	78838.0
52-53	72566.0
54-55	78874.0
56-57	82731.0
58-59	83037.0
60-61	84620.0
62-63	81041.0
64-65	85185.0
66-67	90585.0
68-69	91990.0
70-71	91181.0
72-73	86068.0
74-75	87896.0
76-77	87766.0
78-79	93041.0
80-81	94019.0
82-83	88941.0
84-85	87748.0
86-87	91649.0
88-89	97410.0
90-91	103839.0
92-93	101174.0
94-95	110157.0
96-97	167025.0
98-99	745965.0
100-101	5465555.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.72573644218343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.38652620032568	75.73339260331117
2	7.350927958971592	12.45625569714609
3	1.811754203631791	4.605066274647752
4	0.6901847332674128	2.3390563922893395
5	0.3087977382090234	1.3081557890720035
6	0.16035495972834699	0.8151715233084519
7	0.08620636799514121	0.5112728610075947
8	0.0626895798226985	0.4249136654183338
9	0.029786965902561933	0.22713503602254898
>10	0.11061639139397744	1.39278089112938
>50	0.0016718525099596892	0.09086177006208057
>100	4.410012713611862E-4	0.06284223976763771
>500	2.1023491764076233E-5	0.014053911187927566
>1k	2.1023478668827626E-5	0.019041345629756682
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.308954792728726E-5	0.0	0.0	0.0
3	0.0	3.308954792728726E-5	0.0	0.0	0.0
4	0.0	3.308954792728726E-5	0.0	0.0	2.205969861819151E-5
5	0.0	3.308954792728726E-5	0.0	0.0	2.205969861819151E-5
6	4.411939723638302E-5	3.308954792728726E-5	0.0	0.0	2.205969861819151E-5
7	4.411939723638302E-5	3.308954792728726E-5	0.0	5.514924654547877E-5	2.205969861819151E-5
8	4.411939723638302E-5	4.411939723638302E-5	0.0	6.617909585457453E-5	2.205969861819151E-5
9	4.411939723638302E-5	1.3235819170914905E-4	0.0	6.617909585457453E-5	4.411939723638302E-5
10-11	4.411939723638302E-5	1.3235819170914905E-4	0.0	9.375371912731391E-5	4.411939723638302E-5
12-13	4.411939723638302E-5	1.3235819170914905E-4	0.0	1.1581341774550542E-4	4.411939723638302E-5
14-15	4.411939723638302E-5	1.7096266429098418E-4	0.0	1.2684326705460116E-4	9.375371912731391E-5
16-17	7.720894516367028E-5	1.930223629091757E-4	0.0	1.3787311636369694E-4	9.926864378186179E-5
18-19	7.720894516367028E-5	2.3162683549101084E-4	0.0	1.7647758894553207E-4	9.926864378186179E-5
20-21	7.720894516367028E-5	2.9780593134558534E-4	0.0	2.371417601455587E-4	9.926864378186179E-5
22-23	7.720894516367028E-5	3.198656299637769E-4	0.0	3.2538055461832474E-4	1.1029849309095754E-4
24-25	7.720894516367028E-5	3.5847010254561203E-4	0.0	3.860447258183514E-4	1.213283424000533E-4
26-27	7.720894516367028E-5	3.6398502720015987E-4	0.0	4.797984449456653E-4	1.213283424000533E-4
28-29	7.720894516367028E-5	3.805298011638035E-4	0.0	9.651118145458785E-4	1.213283424000533E-4
30-31	7.720894516367028E-5	4.1913427374563867E-4	0.0	0.0029890891627649494	1.2684326705460116E-4
32-33	7.720894516367028E-5	4.1913427374563867E-4	0.0	0.006684088681312027	1.3235819170914905E-4
34-35	7.720894516367028E-5	5.073730682184046E-4	0.0	0.01114566272684126	1.3235819170914905E-4
36-37	7.720894516367028E-5	6.011267873457186E-4	0.0	0.017989684223135176	1.433880410182448E-4
38-39	7.720894516367028E-5	6.452461845821016E-4	0.0	0.031104175051650026	1.7096266429098418E-4
40-41	7.720894516367028E-5	7.003954311275804E-4	0.0	0.060382910042644705	1.7647758894553207E-4
42-43	8.272386981821816E-5	7.389999037094155E-4	0.0	0.07257640845385006	1.7647758894553207E-4
44-45	9.926864378186179E-5	7.886342256003464E-4	0.0	0.08846490638360249	1.7647758894553207E-4
46-47	1.1029849309095754E-4	8.327536228367294E-4	0.0	0.10587552351801015	1.7647758894553207E-4
48-49	1.1029849309095754E-4	8.382685474912773E-4	0.0	0.12202873783118087	1.7647758894553207E-4
50-51	1.1029849309095754E-4	9.485670405822348E-4	0.0	0.13897058636995197	1.7647758894553207E-4
52-53	1.1029849309095754E-4	9.595968898913306E-4	0.0	0.16206709082319848	1.9853728756372358E-4
54-55	1.1029849309095754E-4	9.761416638549743E-4	0.0	0.20652841338816347	2.3162683549101084E-4
56-57	1.1029849309095754E-4	9.816565885095222E-4	0.0	0.2534769669723295	2.426566848001066E-4
58-59	1.1029849309095754E-4	0.0010368058350550009	0.0	0.3032712216782423	2.4817160945465443E-4
60-61	1.1581341774550542E-4	0.0010368058350550009	0.0	0.33810348579636673	2.647163834182981E-4
62-63	1.213283424000533E-4	0.0010423207597095488	0.0	0.3538706553837191	2.647163834182981E-4
64-65	1.213283424000533E-4	0.0011140147802186711	0.0	0.36884367582081656	2.70231308072846E-4
66-67	1.213283424000533E-4	0.0011526192528005063	0.0	0.37682377179594734	2.7574623272739383E-4
68-69	1.213283424000533E-4	0.00116916402676415	0.0	0.38421377083304153	2.7574623272739383E-4
70-71	1.3235819170914905E-4	0.0012022535746914371	0.0	0.3912122102196628	2.9780593134558534E-4
72-73	1.5441789032734056E-4	0.0012243132733096287	0.0	0.3969587617097016	3.5295517789106414E-4
74-75	1.5441789032734056E-4	0.001257402821236916	0.0	0.40161335811814003	3.5295517789106414E-4
76-77	1.5441789032734056E-4	0.00135115654036423	0.0	0.40571094713646916	3.5847010254561203E-4
78-79	1.5441789032734056E-4	0.001389761012946065	0.0	0.40961551379188904	3.6398502720015987E-4
80-81	1.5441789032734056E-4	0.0014007908622551608	0.0	0.4111541777705079	3.805298011638035E-4
82-83	1.5993281498188845E-4	0.0014118207115642566	0.0	0.41173875978388996	4.081044244365429E-4
84-85	1.6544773963643632E-4	0.0014283654855279002	0.0	0.4122406179274538	4.081044244365429E-4
86-87	1.6544773963643632E-4	0.001444910259491544	0.0	0.4125715134067267	4.081044244365429E-4
88-89	1.6544773963643632E-4	0.0015110893553461184	0.0	0.4127149014477449	4.411939723638302E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10960	0.0	41.24939	1
GTATCAA	18685	0.0	32.711258	1
ATCAACG	20815	0.0	28.556412	3
TCAACGC	21035	0.0	28.277292	4
CAACGCA	21440	0.0	27.763075	5
AACGCAG	21985	0.0	27.266466	6
TATCAAC	22430	0.0	26.780931	2
GTACATG	26405	0.0	23.60982	1
TACATGG	26410	0.0	23.223204	2
ACGCAGA	25790	0.0	23.17834	7
CGCAGAG	26310	0.0	22.736237	8
ACATGGG	27150	0.0	22.079311	3
CATGGGG	19780	0.0	20.303001	4
GCAGAGT	31470	0.0	18.9277	9
GAGTACT	16845	0.0	18.379522	12-13
AGAGTAC	27205	0.0	17.213829	10-11
CAGAGTA	30575	0.0	16.892902	10-11
ATGGGGG	12705	0.0	16.301718	5
GTACTTT	19465	0.0	15.981627	14-15
GAGTACA	18815	0.0	15.705679	1
>>END_MODULE