FastQCFastQC Report
Fri 27 May 2016
SRR2140002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8215855
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83900.042.266051
GTATCAA142900.033.620871
ATCAACG159850.028.810823
TCAACGC159500.028.7938254
CAACGCA164000.027.9524235
AACGCAG169900.027.0814866
TATCAAC178850.026.1285112
TACATGG212500.022.9280452
ACGCAGA200900.022.881577
GTACATG219650.022.876081
CGCAGAG204850.022.3989948
ACATGGG214750.021.95843
CATGGGG161450.019.3926494
GCAGAGT254050.018.1778859
GAGTACT143600.017.30412512-13
AGAGTAC223650.016.89771710-11
CAGAGTA244200.016.4559810-11
ATGGGGG105000.015.5348645
AGGACCG14200.015.216615
GTACTTT165150.015.12371414-15
Produced by FastQC (version 0.11.5)