##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140002_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8215855
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75093085260146	34.0	31.0	34.0	31.0	34.0
2	32.929045242400214	34.0	31.0	34.0	31.0	34.0
3	33.023743724785795	34.0	33.0	34.0	31.0	34.0
4	36.40715835418225	37.0	37.0	37.0	35.0	37.0
5	36.25461062786527	37.0	37.0	37.0	35.0	37.0
6	36.3076626839203	37.0	37.0	37.0	35.0	37.0
7	36.30927188953554	37.0	37.0	37.0	35.0	37.0
8	36.29253352207409	37.0	37.0	37.0	35.0	37.0
9	38.18290743933529	39.0	39.0	39.0	37.0	39.0
10-11	38.08294717956926	39.0	38.0	39.0	37.0	39.0
12-13	38.11962893697613	39.0	38.0	39.0	37.0	39.0
14-15	39.560350931217755	41.0	39.0	41.0	37.0	41.0
16-17	39.542769011867904	41.0	39.0	41.0	37.0	41.0
18-19	39.58729675730645	41.0	39.5	41.0	37.0	41.0
20-21	39.60270540527941	41.0	39.5	41.0	37.0	41.0
22-23	39.54014631864358	41.0	39.0	41.0	37.0	41.0
24-25	39.498612956249424	41.0	39.0	41.0	37.0	41.0
26-27	39.459521891239504	41.0	39.0	41.0	37.0	41.0
28-29	39.36524307857223	40.5	39.0	41.0	36.5	41.0
30-31	39.272070446010076	40.0	39.0	41.0	36.0	41.0
32-33	39.17030510591017	40.0	39.0	41.0	36.0	41.0
34-35	39.097425189460964	40.0	39.0	41.0	35.0	41.0
36-37	39.10004624518261	40.0	39.0	41.0	35.0	41.0
38-39	39.0899038694842	40.0	39.0	41.0	35.0	41.0
40-41	39.04877280363808	40.0	39.0	41.0	35.0	41.0
42-43	39.0105962071014	40.0	39.0	41.0	35.0	41.0
44-45	38.97480310216332	40.0	38.5	41.0	35.0	41.0
46-47	38.90581989356406	40.0	38.0	41.0	35.0	41.0
48-49	38.87592461521613	40.0	38.0	41.0	35.0	41.0
50-51	38.7832597569478	40.0	38.0	41.0	35.0	41.0
52-53	38.61554773161668	40.0	38.0	41.0	35.0	41.0
54-55	38.481911127771525	40.0	38.0	41.0	34.5	41.0
56-57	38.32319123039871	40.0	37.5	41.0	34.0	41.0
58-59	38.135103952587436	40.0	37.0	41.0	34.0	41.0
60-61	38.00749660087866	39.5	37.0	41.0	34.0	41.0
62-63	37.84130785375221	39.0	36.0	41.0	34.0	41.0
64-65	37.59523128944254	39.0	36.0	41.0	33.0	41.0
66-67	37.330768272327774	39.0	35.0	40.0	33.0	41.0
68-69	37.076110551945874	38.0	35.0	40.0	33.0	41.0
70-71	36.78040162127	37.0	35.0	40.0	33.0	41.0
72-73	36.42996919545057	37.0	35.0	39.0	32.5	41.0
74-75	36.052685820156974	36.0	35.0	39.0	32.0	40.5
76-77	34.11910202393847	34.5	32.5	36.5	29.0	39.0
78-79	35.19015237753334	35.0	34.0	37.0	31.0	39.0
80-81	35.11389766765237	35.0	34.0	37.0	32.0	39.0
82-83	34.833549475875216	35.0	34.0	37.0	32.0	39.0
84-85	34.585909281551075	35.0	34.0	36.0	31.0	37.5
86-87	34.37263913705629	35.0	34.0	36.0	31.0	37.0
88-89	34.21008079795034	35.0	34.0	35.5	31.0	37.0
90-91	34.06658100846447	35.0	34.0	35.0	31.0	36.0
92-93	33.97978381098403	35.0	34.0	35.0	31.0	36.0
94-95	33.89566804149832	35.0	34.0	35.0	31.0	36.0
96-97	33.85641139522571	35.0	34.0	35.0	31.0	36.0
98-99	33.88098790943938	35.0	34.0	35.0	31.0	35.5
100-101	32.59484649702243	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	10.0
20	21.0
21	95.0
22	390.0
23	1418.0
24	3622.0
25	7916.0
26	14826.0
27	24741.0
28	37476.0
29	53140.0
30	72405.0
31	96120.0
32	129993.0
33	180988.0
34	278292.0
35	451756.0
36	897658.0
37	1953580.0
38	2730713.0
39	1251947.0
40	28748.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.540831112525716	19.86935017718789	13.030013309631194	24.559805400655197
2	14.873818729894781	21.24044787148336	40.87653129522134	23.00920210340052
3	18.528163336053392	25.870536974968307	30.21091584886332	25.390383840114982
4	12.268952751339727	17.580562551516262	39.227057644173726	30.923427052970283
5	12.942090485563762	38.040266548131584	36.02417619012611	12.993466776178538
6	30.163397479629328	38.14580466670846	18.746338634262656	12.944459219399562
7	26.618446893232655	32.415530702525786	23.33115664772565	17.634865756515907
8	24.574874333692883	36.81750761885078	21.35867132208331	17.248946725373017
9	25.67750360014601	16.360102969470784	21.135093691434108	36.827299738949094
10-11	24.218859830577237	26.7276176732602	29.2204975328572	19.833024963305366
12-13	24.843230946608845	24.186437702106087	29.317168599267696	21.653162752017373
14-15	21.870015725720073	25.691889082552727	26.72472930339442	25.713365888332785
16-17	21.730487945744073	28.825366562115185	27.39230444431651	22.05184104782424
18-19	21.69933622246254	28.219887035494175	28.859053622538372	21.221723119504908
20-21	22.430764386298353	27.500454473445878	28.660820883418474	21.407960256837292
22-23	22.09058143818079	27.46789798933792	28.545134904769455	21.89638566771184
24-25	21.89509301728279	27.524521094512057	28.69259810827226	21.88778777993289
26-27	21.902706481616526	27.7180840423028	28.562696792883074	21.816512683197598
28-29	21.800763393291835	27.84378381401974	28.58566774855281	21.769785044135617
30-31	22.143936499421514	27.671566136002347	28.459386600871273	21.72511076370487
32-33	21.862053043819937	27.817993067946055	28.446201665588788	21.87375222264522
34-35	21.9504140524575	27.858475140935596	28.508856053003523	21.68225475360338
36-37	21.970633087442675	27.79141715054405	28.45545869999159	21.782491062021688
38-39	21.80850704308846	27.88373861148318	28.514650366867638	21.793103978560723
40-41	22.04327751520315	27.745392266627704	28.42706738398358	21.784262834185565
42-43	21.94435332062607	27.84055390637529	28.313935499106623	21.901157273892014
44-45	22.01549231952225	28.008656692101592	28.121912921718028	21.85393806665813
46-47	22.01439802194273	27.898370586095417	27.93690991660779	22.15032147535407
48-49	22.00188215760453	27.95809219592525	27.99946520191413	22.04056044455609
50-51	21.929201475937727	28.0973871946791	27.916933474433247	22.056477854949925
52-53	22.099268582158505	28.001619595415246	27.840161558337968	22.058950264088285
54-55	22.100188048259085	27.933679015078017	27.844631908999105	22.121501027663797
56-57	22.138151931920074	28.058003860989324	27.749724464783938	22.054119742306664
58-59	22.043968892900224	28.160609783717916	27.813420442705134	21.98200088067673
60-61	22.110185015369442	28.17423067672773	27.75064437447629	21.964939933426535
62-63	22.050949240046343	28.213926902172215	27.70669484468462	22.028429013096815
64-65	22.047259259207383	28.129998854953413	27.759599044603945	22.063142841235265
66-67	22.14386020543369	28.14353744578204	27.60933483199511	22.103267516789167
68-69	22.09489942945245	28.153919713917396	27.675571674901704	22.07560918172845
70-71	22.083324043157766	27.907199977761493	27.74078035737399	22.26869562170675
72-73	22.096922214562678	27.966114101001278	27.770885976439995	22.166077707996052
74-75	21.991375118996785	28.11477134418353	27.69107325112087	22.202780285698815
76-77	22.045583635594195	28.063101626516723	27.662018528626987	22.2292962092621
78-79	22.075758384948358	28.099394054560356	27.584610489690032	22.240237070801257
80-81	22.042831085361676	28.09686279238465	27.62037318817695	22.239932934076723
82-83	22.140333729962958	28.017171164359812	27.733532159625835	22.108962946051395
84-85	22.26847047393063	27.993314597773498	27.618470113269538	22.119744815026333
86-87	22.070948206898237	28.038434284985343	27.6761741958229	22.21444331229352
88-89	22.147976648110763	28.039032387399143	27.661053945473324	22.151937019016767
90-91	22.156137952422096	28.079624382986278	27.669594432101434	22.094643232490192
92-93	22.17888196397512	28.022390294455008	27.696828936547753	22.10189880502212
94-95	21.998705644863826	28.125909569509556	27.739774259099047	22.135610526527575
96-97	22.06040058999551	28.121407790769094	27.78677392327653	22.031417695958865
98-99	22.286129072141375	28.669736499831828	27.845818212757962	21.19831621526884
100-101	22.996064573319423	31.329974606537338	24.925325698760247	20.748635121382993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1360.0
1	1177.0
2	1647.5
3	2505.0
4	3521.5
5	5719.0
6	7563.0
7	8765.5
8	9552.5
9	9304.5
10	8284.5
11	7315.0
12	7788.0
13	10410.5
14	16485.0
15	24368.0
16	30448.0
17	34246.0
18	35639.0
19	34781.5
20	33092.5
21	31793.0
22	32564.0
23	36947.5
24	45141.0
25	57347.5
26	72865.5
27	90313.5
28	108772.0
29	128333.0
30	147189.5
31	164754.0
32	183481.5
33	202629.0
34	222075.0
35	240557.0
36	259663.5
37	275057.0
38	285244.0
39	296678.0
40	309930.0
41	321149.0
42	328015.0
43	334227.0
44	341446.5
45	347468.0
46	350913.0
47	353415.5
48	352252.5
49	347160.0
50	340160.5
51	328178.0
52	310824.5
53	292182.0
54	272977.0
55	251607.5
56	230716.5
57	209274.5
58	186229.5
59	163326.5
60	138884.0
61	113729.0
62	90503.0
63	70402.5
64	53514.0
65	40728.0
66	31202.0
67	23787.5
68	17975.5
69	13095.5
70	9560.5
71	7063.5
72	5147.0
73	3819.5
74	2863.0
75	2087.0
76	1451.0
77	935.0
78	620.0
79	418.5
80	281.0
81	188.0
82	134.0
83	94.5
84	71.0
85	50.0
86	33.0
87	23.5
88	18.5
89	14.5
90	11.0
91	10.0
92	8.0
93	4.5
94	2.0
95	0.5
96	0.0
97	0.5
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.9474540385632414E-4
3	0.004272227297098111
4	3.651476322306078E-5
5	3.651476322306078E-5
6	0.0
7	0.0
8	5.964077993099926E-4
9	0.002629062952060376
10-11	9.250406683175396E-4
12-13	3.347186628780571E-4
14-15	1.825738161153039E-4
16-17	0.0015336200553685527
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	7.565811270349744E-4
32-33	8.194862078330773E-4
34-35	6.74706717257403E-5
36-37	8.378225632269574E-4
38-39	6.634931438215023E-4
40-41	3.123367259764958E-5
42-43	9.441062061576748E-5
44-45	9.587132366806479E-4
46-47	0.0
48-49	4.728691334673129E-4
50-51	2.6160288929927117E-5
52-53	2.6394963946458873E-5
54-55	8.4558747156296E-4
56-57	0.0010018139556180281
58-59	1.2227832230881036E-4
60-61	1.1668463266819661E-4
62-63	6.9335669298255765E-6
64-65	7.003342555334811E-6
66-67	0.0
68-69	7.15779085092605E-6
70-71	4.198659122014048E-4
72-73	0.0
74-75	7.397996607722635E-6
76-77	0.0
78-79	1.891164682847522E-4
80-81	0.0
82-83	0.0
84-85	1.5680914711661693E-5
86-87	2.538606654005366E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.676625542954224E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	211.0
20-21	514.0
22-23	1128.0
24-25	2657.0
26-27	3890.0
28-29	8591.0
30-31	17881.0
32-33	22630.0
34-35	30849.0
36-37	49182.0
38-39	59570.0
40-41	59380.0
42-43	65465.0
44-45	78039.0
46-47	78040.0
48-49	75008.0
50-51	69249.0
52-53	66469.0
54-55	71849.0
56-57	75966.0
58-59	75747.0
60-61	74259.0
62-63	71794.0
64-65	73661.0
66-67	78889.0
68-69	78141.0
70-71	77618.0
72-73	72666.0
74-75	73628.0
76-77	73817.0
78-79	78090.0
80-81	79922.0
82-83	75517.0
84-85	73972.0
86-87	77053.0
88-89	82507.0
90-91	88104.0
92-93	86864.0
94-95	96059.0
96-97	148413.0
98-99	683617.0
100-101	5058949.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.21912914798028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.18515503693085	77.75685529359765
2	6.985959860796402	12.04646750921223
3	1.6525167788635324	4.274356727281178
4	0.5755083772195556	1.9847932440494969
5	0.2508560684369774	1.081429588106117
6	0.12307988629734555	0.636710436731374
7	0.07027899281199396	0.42415754903531
8	0.04392998389542325	0.3030083963958553
9	0.025764065664342205	0.19992197744018686
>10	0.08466653859851855	1.0962272476295827
>50	0.0018283707877962516	0.10018296411445006
>100	4.1358903381565656E-4	0.0613747714915862
>500	2.1225444577771167E-5	0.011273042379208766
>1k	2.1225218843930588E-5	0.023241252535677253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.651476322306078E-5	0.0	0.0	0.0
3	0.0	3.651476322306078E-5	0.0	0.0	0.0
4	0.0	7.302952644612155E-5	0.0	1.2171587741020258E-5	0.0
5	0.0	7.302952644612155E-5	0.0	2.4343175482040517E-5	0.0
6	0.0	7.302952644612155E-5	0.0	2.4343175482040517E-5	1.2171587741020258E-5
7	0.0	7.302952644612155E-5	0.0	7.302952644612155E-5	1.2171587741020258E-5
8	0.0	7.302952644612155E-5	0.0	7.302952644612155E-5	1.2171587741020258E-5
9	0.0	1.3388746515122284E-4	0.0	7.302952644612155E-5	2.4343175482040517E-5
10-11	0.0	1.3388746515122284E-4	0.0	7.302952644612155E-5	2.4343175482040517E-5
12-13	0.0	1.3388746515122284E-4	0.0	7.302952644612155E-5	2.4343175482040517E-5
14-15	0.0	1.8865960998581401E-4	0.0	7.302952644612155E-5	6.08579387051013E-5
16-17	0.0	2.1300278546785453E-4	0.0	7.302952644612155E-5	7.302952644612155E-5
18-19	0.0	2.3126016707938492E-4	0.0	8.520111418714181E-5	7.302952644612155E-5
20-21	0.0	2.434317548204052E-4	0.0	1.2780167128071272E-4	7.302952644612155E-5
22-23	0.0	2.616891364319356E-4	0.0	1.7648802224479377E-4	7.911532031663169E-5
24-25	0.0	3.0428969352550646E-4	0.0	2.069169915973444E-4	8.520111418714181E-5
26-27	0.0	3.1646128126652676E-4	0.0	2.251743732088748E-4	8.520111418714181E-5
28-29	0.0	3.28632869007547E-4	0.0	5.233782728638712E-4	8.520111418714181E-5
30-31	0.0	3.7731921997162803E-4	0.0	0.0014362473534403905	8.520111418714181E-5
32-33	0.0	3.8949080771264827E-4	1.2171587741020258E-5	0.002994210584290984	9.737270192816207E-5
34-35	0.0	4.6860612802928E-4	1.2171587741020258E-5	0.005069466294134938	9.737270192816207E-5
36-37	0.0	5.355498606048914E-4	1.2171587741020258E-5	0.007917617825533678	1.034584957986722E-4
38-39	0.0	5.477214483459116E-4	1.2171587741020258E-5	0.012481963228416276	1.0954428966918232E-4
40-41	0.0	5.720646238279521E-4	1.2171587741020258E-5	0.017764432308019065	1.0954428966918232E-4
42-43	0.0	5.781504176984623E-4	1.2171587741020258E-5	0.02260872422894513	1.0954428966918232E-4
44-45	0.0	6.329225625330535E-4	1.2171587741020258E-5	0.027982480216605574	1.0954428966918232E-4
46-47	0.0	6.694373257561143E-4	1.2171587741020258E-5	0.033903957652611935	1.1563008353969246E-4
48-49	0.0	6.694373257561143E-4	1.2171587741020258E-5	0.03991672199667594	1.217158774102026E-4
50-51	0.0	8.094105847778472E-4	1.2171587741020258E-5	0.04617900388943086	1.217158774102026E-4
52-53	0.0	8.276679663893776E-4	1.2171587741020258E-5	0.05198485124189753	1.39973259021733E-4
54-55	0.0	8.398395541303978E-4	1.2171587741020258E-5	0.058034130349184594	1.5823064063326338E-4
56-57	0.0	8.520111418714181E-4	1.2171587741020258E-5	0.06382780611391024	1.5823064063326338E-4
58-59	0.0	8.641827296124384E-4	1.2171587741020258E-5	0.06959105290928333	1.5823064063326338E-4
60-61	0.0	8.763543173534586E-4	1.2171587741020258E-5	0.0750378384233899	1.5823064063326338E-4
62-63	0.0	8.763543173534586E-4	1.2171587741020258E-5	0.08072805569231686	1.5823064063326338E-4
64-65	0.0	9.980701947636611E-4	1.2171587741020258E-5	0.08602878215353119	1.5823064063326338E-4
66-67	0.0	0.0010102417825046815	1.2171587741020258E-5	0.0917981147427748	1.5823064063326338E-4
68-69	0.0	0.001028499164116212	1.2171587741020258E-5	0.09753093256879533	1.5823064063326338E-4
70-71	0.0	0.0010528423395982523	1.2171587741020258E-5	0.10301423284612496	1.7040222837428362E-4
72-73	0.0	0.0010589281334687625	1.2171587741020258E-5	0.10765160777545368	1.825738161153039E-4
74-75	0.0	0.0010589281334687625	1.2171587741020258E-5	0.11169866069934292	1.825738161153039E-4
76-77	0.0	0.0010710997212097827	1.2171587741020258E-5	0.11526493590746184	1.825738161153039E-4
78-79	0.0	0.0010832713089508031	1.2171587741020258E-5	0.11850866404044375	1.825738161153039E-4
80-81	0.0	0.0010954428966918233	1.2171587741020258E-5	0.12000576933258925	1.8865960998581401E-4
82-83	0.0	0.0011137002783033537	1.2171587741020258E-5	0.12054740498706465	1.9474540385632414E-4
84-85	0.0	0.0011502150415264145	1.2171587741020258E-5	0.12096732476412984	1.9474540385632414E-4
86-87	0.0	0.001174558217008455	1.2171587741020258E-5	0.1211864133434682	1.9474540385632414E-4
88-89	0.0	0.0012536735373250866	1.2171587741020258E-5	0.1213020434270079	2.069169915973444E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8390	0.0	42.26605	1
GTATCAA	14290	0.0	33.62087	1
ATCAACG	15985	0.0	28.81082	3
TCAACGC	15950	0.0	28.793825	4
CAACGCA	16400	0.0	27.952423	5
AACGCAG	16990	0.0	27.081486	6
TATCAAC	17885	0.0	26.128511	2
TACATGG	21250	0.0	22.928045	2
ACGCAGA	20090	0.0	22.88157	7
GTACATG	21965	0.0	22.87608	1
CGCAGAG	20485	0.0	22.398994	8
ACATGGG	21475	0.0	21.9584	3
CATGGGG	16145	0.0	19.392649	4
GCAGAGT	25405	0.0	18.177885	9
GAGTACT	14360	0.0	17.304125	12-13
AGAGTAC	22365	0.0	16.897717	10-11
CAGAGTA	24420	0.0	16.45598	10-11
ATGGGGG	10500	0.0	15.534864	5
AGGACCG	1420	0.0	15.21661	5
GTACTTT	16515	0.0	15.123714	14-15
>>END_MODULE