FastQCFastQC Report
Fri 27 May 2016
SRR2140001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9183946
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96450.041.4170881
GTATCAA166200.034.6190531
TCAACGC188950.029.3122844
ATCAACG189350.029.207083
CAACGCA190700.028.955135
AACGCAG198900.027.9313746
TATCAAC205800.027.4663332
GTACATG271400.024.3551621
TACATGG265300.023.866282
ACGCAGA231950.023.8241947
CGCAGAG233600.023.6739718
ACATGGG270750.022.9345463
CATGGGG190250.021.2630624
GCAGAGT293400.018.84859
GAGTACT156700.017.73920212-13
AGAGTAC251650.017.65046510-11
CAGAGTA279550.016.83196410-11
ATGGGGG121050.016.7616525
GAGTACA188800.016.5333751
AGTACAT193400.015.4643282
Produced by FastQC (version 0.11.5)