##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140001_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9183946
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74120949752971	34.0	31.0	34.0	31.0	34.0
2	32.92430399743204	34.0	31.0	34.0	31.0	34.0
3	33.02071397196804	34.0	33.0	34.0	31.0	34.0
4	36.40661225577764	37.0	37.0	37.0	35.0	37.0
5	36.249733284581595	37.0	37.0	37.0	35.0	37.0
6	36.303005374813836	37.0	37.0	37.0	35.0	37.0
7	36.30694278907999	37.0	37.0	37.0	35.0	37.0
8	36.29145924856265	37.0	37.0	37.0	35.0	37.0
9	38.19195931683396	39.0	39.0	39.0	37.0	39.0
10-11	38.09045823004621	39.0	38.0	39.0	37.0	39.0
12-13	38.129396448977374	39.0	38.0	39.0	37.0	39.0
14-15	39.56940916246677	41.0	39.0	41.0	37.0	41.0
16-17	39.553184382835006	41.0	39.0	41.0	37.0	41.0
18-19	39.59985968994155	41.0	39.5	41.0	37.0	41.0
20-21	39.61642859172787	41.0	40.0	41.0	37.0	41.0
22-23	39.55338359304984	41.0	39.0	41.0	37.0	41.0
24-25	39.510373815871205	41.0	39.0	41.0	37.0	41.0
26-27	39.47570122366764	41.0	39.0	41.0	37.0	41.0
28-29	39.38260941065008	40.5	39.0	41.0	36.5	41.0
30-31	39.289497888666425	40.0	39.0	41.0	36.0	41.0
32-33	39.18694691422789	40.0	39.0	41.0	36.0	41.0
34-35	39.11404566795044	40.0	39.0	41.0	35.0	41.0
36-37	39.11738373046799	40.0	39.0	41.0	35.0	41.0
38-39	39.10870206334602	40.0	39.0	41.0	35.0	41.0
40-41	39.06285056629487	40.0	39.0	41.0	35.0	41.0
42-43	39.02523097525792	40.0	39.0	41.0	35.0	41.0
44-45	38.98635715871127	40.0	38.5	41.0	35.0	41.0
46-47	38.914430102842275	40.0	38.0	41.0	35.0	41.0
48-49	38.88125732441291	40.0	38.0	41.0	35.0	41.0
50-51	38.78083127268505	40.0	38.0	41.0	35.0	41.0
52-53	38.60970585038926	40.0	38.0	41.0	35.0	41.0
54-55	38.4721271253583	40.0	38.0	41.0	34.5	41.0
56-57	38.308415924442095	40.0	37.0	41.0	34.0	41.0
58-59	38.11761318802458	40.0	37.0	41.0	34.0	41.0
60-61	37.984311462052275	39.5	36.5	41.0	34.0	41.0
62-63	37.814386805606915	39.0	36.0	41.0	34.0	41.0
64-65	37.5603426433963	39.0	35.5	41.0	33.0	41.0
66-67	37.29149933700059	39.0	35.0	40.0	33.0	41.0
68-69	37.03502807554874	38.0	35.0	40.0	33.0	41.0
70-71	36.737328980858635	37.0	35.0	40.0	33.0	41.0
72-73	36.38867793506391	37.0	35.0	39.0	33.0	41.0
74-75	36.01055373089839	36.0	35.0	39.0	32.0	40.5
76-77	34.08662616522925	34.5	32.5	36.5	29.0	39.0
78-79	35.149891235835966	35.0	34.0	37.0	31.5	39.0
80-81	35.07836031012623	35.0	34.0	37.0	32.0	39.0
82-83	34.805925412962615	35.0	34.0	36.5	32.0	39.0
84-85	34.56394402943811	35.0	34.0	36.0	31.0	37.0
86-87	34.36161744588675	35.0	34.0	36.0	31.0	37.0
88-89	34.20894660719715	35.0	34.0	35.5	31.0	37.0
90-91	34.069111068097584	35.0	34.0	35.0	31.0	36.0
92-93	33.98582950773751	35.0	34.0	35.0	31.0	36.0
94-95	33.90649470498215	35.0	34.0	35.0	31.0	36.0
96-97	33.86887325667169	35.0	34.0	35.0	31.0	36.0
98-99	33.895132008972354	35.0	34.0	35.0	31.0	35.0
100-101	32.6054193539106	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	9.0
20	34.0
21	104.0
22	444.0
23	1545.0
24	3859.0
25	8574.0
26	16046.0
27	26708.0
28	41069.0
29	57459.0
30	78625.0
31	105374.0
32	142606.0
33	198740.0
34	307630.0
35	505860.0
36	1017620.0
37	2218148.0
38	3044182.0
39	1379411.0
40	29897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81742292474281	19.717330655036516	12.850053778626311	24.615192641594366
2	15.015046928690905	21.24458864795849	40.518649197609655	23.22171522574095
3	18.606998144823137	25.829022971765315	30.05847122842501	25.50550765498654
4	12.323391450358837	17.51861210780862	38.90652172090391	31.25147472092863
5	13.030710229799988	38.04024045009861	35.790618474004035	13.138430846097368
6	30.314768836837676	37.87931679911881	18.729530857433176	13.07638350661034
7	26.887222551177892	32.30488289020863	23.14615090289076	17.661743655722713
8	24.86229420623568	36.45268655736441	21.354674178950948	17.330345057448962
9	25.94949789278739	16.10024983405374	20.97841653295144	36.971835740207425
10-11	24.400636333395582	26.598322166103266	29.001041500501152	20.0
12-13	24.989076016659986	24.08573092037704	29.152118142131233	21.773074920831746
14-15	22.06761405665666	25.517252493171906	26.570608334653116	25.844525115518312
16-17	21.838005041589216	28.67820497820594	27.233025986326698	22.250763993878138
18-19	21.8419838269955	28.03312432368396	28.729676764214425	21.39521508510612
20-21	22.668273073302927	27.303905726310816	28.49007431037179	21.537746890014464
22-23	22.31236976824935	27.28675778485651	28.3652940677782	22.035578379115943
24-25	22.086362724925962	27.314408342436074	28.527945773250607	22.07128315938736
26-27	22.08233613905077	27.55828169658735	28.34672546665097	22.012656697710906
28-29	21.976476228844586	27.668183822339454	28.360467292159747	21.994872656656213
30-31	22.25509132160253	27.485923282812447	28.35923514797508	21.89975024760994
32-33	21.97299099397034	27.624945707419872	28.34376907216675	22.05829422644304
34-35	22.09636583766548	27.69190018966732	28.2975216463718	21.914212326295402
36-37	22.10876676272804	27.60312079088264	28.310089862501936	21.978022583887384
38-39	21.946142855168212	27.70301735927092	28.37322152143023	21.97761826413064
40-41	22.177599665444564	27.6013044350411	28.2660912784562	21.95500462105813
42-43	22.104054613051836	27.625094161260456	28.16433845473993	22.106512770947777
44-45	22.185191395554188	27.840575103013364	27.942184494957566	22.03204900647488
46-47	22.206477369619165	27.736554798028994	27.744524659302805	22.312443173049033
48-49	22.171066874867304	27.789939084230962	27.837759223796045	22.20123481710569
50-51	22.09080742326901	27.886112608529405	27.767757709927203	22.255322258274383
52-53	22.280691787793785	27.86086712974863	27.601426862232987	22.2570142202246
54-55	22.23090610997306	27.79484658996464	27.661795956341788	22.312451343720515
56-57	22.31755954390958	27.895137188486274	27.562995625370075	22.224307642234066
58-59	22.194027996977617	27.998748449147993	27.631422914884894	22.1758006389895
60-61	22.30828188005471	27.96473295510734	27.569103118456965	22.157882046380976
62-63	22.24813219539077	28.00325942845706	27.525843373369952	22.222765002782218
64-65	22.20265780730897	27.977103519577373	27.55477314706828	22.26546552604538
66-67	22.320827166451842	27.959135814092416	27.411129816884493	22.308907202571245
68-69	22.272854640190143	27.94349914857269	27.484234648915617	22.299411562321545
70-71	22.22139708739733	27.70927092268744	27.58538758732571	22.48394440258952
72-73	22.30410525604177	27.723510379729188	27.604584235149215	22.367800129079825
74-75	22.177624924891425	27.902271705151026	27.543779928692835	22.376323441264713
76-77	22.257365062589308	27.860294166303508	27.411514520398022	22.470826250709163
78-79	22.276771885985966	27.87710079627388	27.41096009639946	22.435167221340695
80-81	22.199242988072744	27.886579228747664	27.441927935071046	22.472249848108547
82-83	22.30665635712571	27.83258758667856	27.537912533692033	22.322843522503696
84-85	22.449470162147318	27.824478057240647	27.450092908678563	22.27595887193347
86-87	22.228903625284975	27.833499850224484	27.540578402549947	22.397018121940587
88-89	22.301307665567347	27.819234384460785	27.499213429600296	22.380244520371573
90-91	22.34101562275378	27.864558567301607	27.484594652815158	22.30983115712945
92-93	22.363965307194494	27.810650179231793	27.48133592874213	22.344048584831583
94-95	22.214491195845387	27.92323179417974	27.53552179187385	22.32675521810102
96-97	22.263816307287744	27.918120456460045	27.58978637454139	22.228276861710825
98-99	22.503281263944718	28.473908418937256	27.600235494299046	21.422574822818977
100-101	23.204483283487452	31.09440457841332	24.684859764158492	21.016252373940734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1533.0
1	1370.5
2	1924.5
3	2890.0
4	4132.5
5	6801.0
6	8933.5
7	10235.0
8	10960.0
9	10478.0
10	9301.0
11	8310.0
12	9102.5
13	12337.0
14	19251.5
15	28177.5
16	35349.0
17	39660.0
18	40672.5
19	38974.0
20	36395.0
21	34259.0
22	34486.0
23	38735.0
24	47203.5
25	59749.5
26	76337.0
27	95651.5
28	116506.5
29	137742.0
30	157042.0
31	176658.0
32	197374.5
33	217400.0
34	238207.5
35	259365.5
36	281005.0
37	299662.0
38	313779.5
39	325775.5
40	339370.0
41	352407.5
42	360287.5
43	368797.5
44	380006.5
45	389500.0
46	395626.0
47	399669.0
48	401159.0
49	396767.0
50	390181.5
51	379815.0
52	362166.5
53	344160.5
54	324683.0
55	301089.0
56	276095.0
57	248592.5
58	219657.5
59	191378.5
60	161853.0
61	132126.5
62	104771.5
63	81846.0
64	62281.5
65	46523.5
66	35131.0
67	26865.5
68	20739.5
69	15271.5
70	10941.0
71	8083.0
72	5942.0
73	4367.0
74	3205.0
75	2280.0
76	1580.5
77	1073.5
78	737.5
79	505.0
80	339.5
81	222.0
82	151.5
83	108.0
84	75.0
85	56.0
86	41.5
87	32.0
88	24.0
89	16.5
90	11.0
91	7.0
92	5.0
93	3.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.3066278917580744E-4
3	0.004954297422916032
4	5.4442828823253104E-5
5	3.266569729395186E-5
6	0.0
7	0.0
8	5.988711170557841E-4
9	0.002722141441162655
10-11	8.819738269367003E-4
12-13	2.722141441162655E-4
14-15	1.3610707205813274E-4
16-17	0.001475400661110159
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	6.932211486816353E-4
32-33	7.277259368801164E-4
34-35	2.744266924525305E-5
36-37	7.71866566324013E-4
38-39	6.772440718245095E-4
40-41	3.356293363310254E-5
42-43	9.584551417059014E-5
44-45	0.0010696808908621088
46-47	0.0
48-49	4.880768065552899E-4
50-51	3.521417938842478E-5
52-53	2.961916506891017E-5
54-55	6.756525263754118E-4
56-57	8.879904010049876E-4
58-59	1.343388063777226E-4
60-61	1.543256460025244E-4
62-63	1.24777124659647E-5
64-65	6.304096635245157E-6
66-67	0.0
68-69	6.448985045835517E-6
70-71	4.6984609342876516E-4
72-73	0.0
74-75	1.3351149997967287E-5
76-77	0.0
78-79	2.5960781184500265E-4
80-81	0.0
82-83	0.0
84-85	2.835498484532391E-5
86-87	3.1569350436937745E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	2.2785467155622538E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	202.0
20-21	600.0
22-23	1177.0
24-25	2670.0
26-27	4362.0
28-29	9978.0
30-31	20911.0
32-33	26271.0
34-35	35746.0
36-37	57554.0
38-39	69289.0
40-41	68744.0
42-43	76771.0
44-45	90877.0
46-47	91431.0
48-49	87631.0
50-51	80143.0
52-53	77045.0
54-55	83313.0
56-57	88886.0
58-59	88485.0
60-61	86797.0
62-63	82715.0
64-65	85645.0
66-67	90819.0
68-69	90977.0
70-71	90131.0
72-73	84154.0
74-75	84949.0
76-77	84678.0
78-79	90012.0
80-81	90967.0
82-83	85637.0
84-85	84067.0
86-87	87709.0
88-89	94424.0
90-91	101059.0
92-93	98363.0
94-95	107601.0
96-97	164632.0
98-99	754294.0
100-101	5582230.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.24938399736558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.6299635740828	76.40899182396875
2	7.24701904116941	12.356078181537422
3	1.7970758793585402	4.595988351355188
4	0.6329193937127806	2.158239537360026
5	0.29463447698582396	1.255870383371374
6	0.14002265387065868	0.7162106992889993
7	0.08285390352121957	0.49442709658727896
8	0.049926370873079794	0.3404953889723254
9	0.030344213425640944	0.23281429521784325
>10	0.09292841344953233	1.1987853832117763
>50	0.0018653956447003018	0.10475726198967308
>100	3.4699397686232094E-4	0.05279895456471709
>500	5.9814544989569126E-5	0.03180169748897511
>1k	3.9875384004742006E-5	0.05274094508572444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.088856576465062E-5
2	0.0	2.177713152930124E-5	0.0	0.0	1.088856576465062E-5
3	0.0	2.177713152930124E-5	0.0	0.0	1.088856576465062E-5
4	0.0	1.1977422341115681E-4	0.0	1.088856576465062E-5	1.088856576465062E-5
5	1.088856576465062E-5	1.1977422341115681E-4	0.0	1.088856576465062E-5	1.088856576465062E-5
6	1.088856576465062E-5	1.1977422341115681E-4	0.0	1.088856576465062E-5	1.088856576465062E-5
7	1.088856576465062E-5	1.1977422341115681E-4	0.0	3.266569729395186E-5	2.177713152930124E-5
8	1.088856576465062E-5	1.3066278917580744E-4	0.0	3.266569729395186E-5	4.355426305860248E-5
9	1.088856576465062E-5	1.4155135494045806E-4	0.0	6.533139458790372E-5	6.533139458790372E-5
10-11	1.088856576465062E-5	1.4155135494045806E-4	0.0	8.710852611720496E-5	6.533139458790372E-5
12-13	1.088856576465062E-5	1.4155135494045806E-4	0.0	1.088856576465062E-4	6.533139458790372E-5
14-15	1.088856576465062E-5	1.9054990088138586E-4	0.0	1.2521850629348212E-4	7.077567747022903E-5
16-17	1.088856576465062E-5	1.9599418376371115E-4	0.0	1.3610707205813274E-4	8.166424323487965E-5
18-19	1.088856576465062E-5	2.177713152930124E-4	0.0	1.5243992070510868E-4	9.255280899953027E-5
20-21	1.088856576465062E-5	2.667698612339402E-4	0.0	1.8510561799906053E-4	9.799709188185558E-5
22-23	1.088856576465062E-5	2.9399127564556677E-4	0.0	2.3954844682231362E-4	1.3066278917580744E-4
24-25	1.088856576465062E-5	3.103241242925427E-4	0.0	2.9943555852789204E-4	1.633284864697593E-4
26-27	1.088856576465062E-5	3.1576840717486795E-4	0.0	3.4843410446881983E-4	1.9054990088138586E-4
28-29	1.088856576465062E-5	3.1576840717486795E-4	0.0	7.73088169290194E-4	2.0688274952836177E-4
30-31	1.088856576465062E-5	3.7021123599812107E-4	0.0	0.0020851603439305936	2.28659881057663E-4
32-33	1.088856576465062E-5	3.810998017627717E-4	0.0	0.004339093457213272	2.5043701258696424E-4
34-35	1.088856576465062E-5	4.1376549905672354E-4	0.0	0.007224563384845686	2.722141441162655E-4
36-37	1.088856576465062E-5	4.57319762115326E-4	0.0	0.011846759551939874	2.885469927632414E-4
38-39	1.088856576465062E-5	4.7909689364462725E-4	0.0	0.019191097160196718	3.0487984141021736E-4
40-41	1.088856576465062E-5	5.335397224678804E-4	0.0	0.026889313155804704	3.1576840717486795E-4
42-43	1.088856576465062E-5	5.60761136879507E-4	0.0	0.033738221021769946	3.1576840717486795E-4
44-45	1.088856576465062E-5	6.152039657027601E-4	0.0	0.041360217057025375	3.3754553870416924E-4
46-47	1.088856576465062E-5	6.696467945260131E-4	0.0	0.05021806530656866	3.974326504097476E-4
48-49	1.088856576465062E-5	6.750910774083384E-4	0.0	0.05919024349664077	4.57319762115326E-4
50-51	1.088856576465062E-5	7.621996035255434E-4	0.0	0.06790654039124358	4.57319762115326E-4
52-53	1.088856576465062E-5	7.621996035255434E-4	0.0	0.07564286636702786	4.954297422916032E-4
54-55	1.088856576465062E-5	7.621996035255434E-4	0.0	0.08342274660587073	5.335397224678804E-4
56-57	1.088856576465062E-5	7.621996035255434E-4	0.0	0.09073986279971594	5.389840053502057E-4
58-59	1.088856576465062E-5	7.676438864078687E-4	0.0	0.09765410206026909	5.498725711148562E-4
60-61	1.088856576465062E-5	7.839767350548446E-4	0.0	0.10402935731547203	5.662054197618322E-4
62-63	1.088856576465062E-5	7.948653008194952E-4	0.0	0.1105243867940861	5.879825512911334E-4
64-65	1.088856576465062E-5	8.710852611720495E-4	0.0	0.11675809069434859	5.879825512911334E-4
66-67	1.088856576465062E-5	8.710852611720495E-4	0.0	0.12338922724502083	5.988711170557841E-4
68-69	1.088856576465062E-5	8.928623927013508E-4	0.0	0.13033613220286794	6.097596828204347E-4
70-71	1.088856576465062E-5	9.037509584660015E-4	0.0	0.13650994899142482	6.206482485850854E-4
72-73	1.088856576465062E-5	9.091952413483268E-4	0.0	0.1414914678287525	6.642025116436878E-4
74-75	1.088856576465062E-5	9.14639524230652E-4	0.0	0.14578700702290714	6.642025116436878E-4
76-77	1.088856576465062E-5	9.854152017008812E-4	0.0	0.14938023372524184	6.642025116436878E-4
78-79	1.088856576465062E-5	0.001001748050347857	0.0	0.15291901759875332	6.642025116436878E-4
80-81	1.088856576465062E-5	0.001001748050347857	0.0	0.15450330391750997	6.914239260553143E-4
82-83	1.088856576465062E-5	0.001001748050347857	0.0	0.15499873365980157	7.621996035255434E-4
84-85	1.633284864697593E-5	0.0010126366161125077	0.0	0.15546149770479922	7.621996035255434E-4
86-87	2.177713152930124E-5	0.0010235251818771583	0.0	0.15576093326332713	7.621996035255434E-4
88-89	2.177713152930124E-5	0.0010507465962887848	0.0	0.15588070748673827	7.839767350548446E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9645	0.0	41.417088	1
GTATCAA	16620	0.0	34.619053	1
TCAACGC	18895	0.0	29.312284	4
ATCAACG	18935	0.0	29.20708	3
CAACGCA	19070	0.0	28.95513	5
AACGCAG	19890	0.0	27.931374	6
TATCAAC	20580	0.0	27.466333	2
GTACATG	27140	0.0	24.355162	1
TACATGG	26530	0.0	23.86628	2
ACGCAGA	23195	0.0	23.824194	7
CGCAGAG	23360	0.0	23.673971	8
ACATGGG	27075	0.0	22.934546	3
CATGGGG	19025	0.0	21.263062	4
GCAGAGT	29340	0.0	18.8485	9
GAGTACT	15670	0.0	17.739202	12-13
AGAGTAC	25165	0.0	17.650465	10-11
CAGAGTA	27955	0.0	16.831964	10-11
ATGGGGG	12105	0.0	16.761652	5
GAGTACA	18880	0.0	16.533375	1
AGTACAT	19340	0.0	15.464328	2
>>END_MODULE