FastQCFastQC Report
Fri 27 May 2016
SRR2140000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8028581
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107100.040.1501271
GTATCAA179950.034.0364881
ATCAACG211300.028.2071273
TCAACGC213550.028.0475184
CAACGCA215450.027.6825435
TATCAAC223100.027.0560252
AACGCAG222400.026.89366
GTACATG265900.023.6699071
TACATGG265800.022.9956052
ACGCAGA261750.022.802447
CGCAGAG264700.022.5642748
ACATGGG275050.021.654033
CATGGGG217100.019.1643544
GCAGAGT310650.019.0633779
GAGTACT155250.018.48825512-13
AGAGTAC266850.017.6735110-11
CAGAGTA296400.017.09438110-11
GAGTACA195800.016.4819761
AGTACTT169650.016.22178512-13
GTACTTT179750.015.98044714-15
Produced by FastQC (version 0.11.5)