##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8028581
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71974300315336	34.0	31.0	34.0	31.0	34.0
2	32.90705343821031	34.0	31.0	34.0	31.0	34.0
3	33.00087001675639	34.0	33.0	34.0	31.0	34.0
4	36.39722038053798	37.0	37.0	37.0	35.0	37.0
5	36.22934376572896	37.0	37.0	37.0	35.0	37.0
6	36.282731531263124	37.0	37.0	37.0	35.0	37.0
7	36.284330942167735	37.0	37.0	37.0	35.0	37.0
8	36.272535208899306	37.0	37.0	37.0	35.0	37.0
9	38.170249886001024	39.0	39.0	39.0	37.0	39.0
10-11	38.07101535128063	39.0	38.0	39.0	36.0	39.0
12-13	38.10451056045894	39.0	38.0	39.0	37.0	39.0
14-15	39.528813622232875	41.0	39.0	41.0	37.0	41.0
16-17	39.5120327614556	41.0	39.0	41.0	37.0	41.0
18-19	39.55907918223656	41.0	39.5	41.0	37.0	41.0
20-21	39.57258282200789	41.0	39.5	41.0	37.0	41.0
22-23	39.50999383381391	41.0	39.0	41.0	37.0	41.0
24-25	39.46131055114881	41.0	39.0	41.0	37.0	41.0
26-27	39.41522089383272	41.0	39.0	41.0	36.5	41.0
28-29	39.321185852318294	40.0	39.0	41.0	36.0	41.0
30-31	39.22122604197021	40.0	39.0	41.0	36.0	41.0
32-33	39.11388278477314	40.0	39.0	41.0	35.5	41.0
34-35	39.036209065981595	40.0	39.0	41.0	35.0	41.0
36-37	39.03120853727168	40.0	39.0	41.0	35.0	41.0
38-39	39.018703495991616	40.0	39.0	41.0	35.0	41.0
40-41	38.9633196086962	40.0	38.5	41.0	35.0	41.0
42-43	38.913539748361316	40.0	38.0	41.0	35.0	41.0
44-45	38.86430806301246	40.0	38.0	41.0	35.0	41.0
46-47	38.77138188282085	40.0	38.0	41.0	35.0	41.0
48-49	38.71960990634834	40.0	38.0	41.0	35.0	41.0
50-51	38.60246580420227	40.0	38.0	41.0	35.0	41.0
52-53	38.40740153191766	40.0	37.5	41.0	34.0	41.0
54-55	38.24084190871186	40.0	37.0	41.0	34.0	41.0
56-57	38.04492560707695	40.0	37.0	41.0	34.0	41.0
58-59	37.82077769969365	39.0	36.0	41.0	33.5	41.0
60-61	37.65330748594823	39.0	36.0	41.0	33.0	41.0
62-63	37.442666269517694	39.0	35.0	40.5	33.0	41.0
64-65	37.15466475154648	38.5	35.0	40.0	33.0	41.0
66-67	36.85607995061291	37.5	35.0	40.0	33.0	41.0
68-69	36.57880045614193	37.0	35.0	40.0	33.0	41.0
70-71	36.27653804009725	36.5	35.0	39.0	32.5	41.0
72-73	35.9317479535515	36.0	35.0	39.0	32.0	41.0
74-75	35.56344365337096	35.5	34.5	38.5	32.0	40.0
76-77	33.69621423706735	34.5	32.0	36.0	29.0	39.0
78-79	34.76368543388213	35.0	34.0	37.0	31.0	39.0
80-81	34.72701223957907	35.0	34.0	36.5	31.0	39.0
82-83	34.49943060659523	35.0	34.0	36.0	31.0	37.5
84-85	34.30122079173662	35.0	34.0	36.0	31.0	37.0
86-87	34.136406440536824	35.0	34.0	35.0	31.0	37.0
88-89	34.019203179107166	35.0	34.0	35.0	31.0	36.0
90-91	33.90629450301937	35.0	34.0	35.0	31.0	36.0
92-93	33.844124782060135	35.0	34.0	35.0	31.0	36.0
94-95	33.78345014750438	35.0	34.0	35.0	31.0	36.0
96-97	33.76053518492319	35.0	34.0	35.0	31.0	35.0
98-99	33.79719076205375	35.0	34.0	35.0	31.0	35.0
100-101	32.48909665722222	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	7.0
20	37.0
21	114.0
22	434.0
23	1480.0
24	3792.0
25	8566.0
26	15714.0
27	26099.0
28	39145.0
29	55190.0
30	74063.0
31	99364.0
32	133547.0
33	189517.0
34	301427.0
35	502464.0
36	1019560.0
37	2107469.0
38	2439431.0
39	993457.0
40	17701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.616711346625266	19.158092818643794	12.838582558985207	24.386613275745738
2	15.596369812944202	20.677226708078788	39.86478533466807	23.86161814430894
3	19.02841824685947	25.478143446334773	29.376483133738663	26.116955173067097
4	13.035272248709548	16.83256985234496	37.47829316723335	32.65386473171214
5	13.737771810208033	37.55418766456833	34.91938798641852	13.788652538805119
6	31.621465860530023	36.51734970351548	18.083930398161268	13.777254037793229
7	27.879671389003864	31.8365225436475	22.420524872328	17.86328119502064
8	25.806940192597956	35.71657753877481	20.520797186237395	17.955685082389845
9	26.4041440089702	16.003515536981958	20.43829369406782	37.15404675998003
10-11	25.426648282184367	25.881838597697453	27.974200692282874	20.717312427835303
12-13	25.936004070469693	23.415127810942376	28.353200434822817	22.29566768376511
14-15	22.970333076708172	24.69932694124521	25.748187317518894	26.582152664527726
16-17	22.710310017282936	27.860831485371474	26.38336503625642	23.04549346108917
18-19	22.760958630173874	27.22532163529271	27.955189092568162	22.05853064196525
20-21	23.491983339525977	26.385058535740193	27.797174335974432	22.3257837887594
22-23	23.358152006828366	26.280909880448828	27.6520138042167	22.708924308506106
24-25	23.083024946819133	26.43570893429819	27.696007918351466	22.785258200531207
26-27	22.98219834963737	26.584065440154035	27.688171104843544	22.745565105365053
28-29	22.889998556927104	26.74113230458991	27.615501022561205	22.753368115921784
30-31	23.10822553864452	26.574464053496662	27.54919101365833	22.76811939420049
32-33	22.87919274878378	26.747597066360147	27.520601739399826	22.852608445456255
34-35	23.026624801050506	26.760591950856533	27.557061914985802	22.655721333107163
36-37	23.089881817441423	26.66808735303356	27.474118683962452	22.767912145562565
38-39	22.953343822206673	26.785409300113187	27.510947928276025	22.75029894940411
40-41	23.07242306792946	26.607343361760204	27.504759095571163	22.81547447473917
42-43	22.909944634273582	26.77872518487236	27.272109591940303	23.039220588913757
44-45	23.02252205226349	26.8441089946429	27.17481398129429	22.958554971799323
46-47	23.17820418785233	26.874097556280823	26.87805392484269	23.069644331024158
48-49	23.09353431258334	27.018487250486388	26.765728494115358	23.122249942814914
50-51	23.02852325027	27.087989747313294	26.78384669474737	23.09964030766933
52-53	23.261094869184067	26.925659019412823	26.631377286009954	23.181868825393153
54-55	23.098185322495198	26.90471148857465	26.723184843117476	23.273918345812678
56-57	23.182822635809252	26.951757222307144	26.712829213332384	23.152590928551223
58-59	23.159544367992385	27.078788016008602	26.637489983443114	23.124177632555902
60-61	23.216754256228388	27.000038560405866	26.70486783456202	23.07833934880373
62-63	23.18712917010596	27.09016910471089	26.590108881422342	23.13259284376081
64-65	23.17087479540732	27.06120230945584	26.550252141781733	23.217670753355105
66-67	23.212381012519845	26.962515479652367	26.517501193179577	23.30760231464821
68-69	23.114830777823904	27.025010239050623	26.625899005583992	23.234259977541477
70-71	23.159562576984534	26.86707585019273	26.6038728421328	23.369488730689934
72-73	23.179641500095553	26.985779186291786	26.547917742996297	23.286661570616367
74-75	23.009291150392183	27.086265434144423	26.62466382259554	23.27977959286785
76-77	23.246053018392438	26.962782476430895	26.47557669350271	23.315587811673957
78-79	23.133986967064217	26.96783406121635	26.471846631779083	23.42633233994034
80-81	23.127566005763846	27.091224210996355	26.52442962884214	23.256780154397656
82-83	23.162064209475165	26.99487802128564	26.669824459100973	23.173233310138222
84-85	23.252191545538786	26.97473341526993	26.55603610379842	23.21703893539286
86-87	23.100329538153762	27.04397468078622	26.635723648900516	23.2199721321595
88-89	23.149254436647535	26.995602766434505	26.53561173254379	23.319531064374168
90-91	23.234482234872022	26.95440743256653	26.55145343799838	23.259656894563072
92-93	23.220224633768353	26.950935202085763	26.57756444738693	23.25127571675895
94-95	23.082617264042803	26.959152184329998	26.688962141333434	23.26926841029377
96-97	23.162828506097092	26.964041267697027	26.631845153011852	23.24128507319403
98-99	23.373744082485327	27.63549755399641	26.64920548770854	22.341552875809725
100-101	24.22912674983768	30.027670385473314	23.626749114190055	22.11645375049895
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1736.0
1	1583.0
2	2289.0
3	3333.0
4	4479.0
5	7202.0
6	9580.0
7	11332.5
8	12415.5
9	12001.5
10	10392.5
11	8719.5
12	9068.0
13	11831.0
14	18541.0
15	27600.5
16	34712.5
17	38445.5
18	38754.5
19	36232.0
20	32663.0
21	29543.5
22	28096.5
23	29976.0
24	35635.5
25	44588.0
26	55188.5
27	66218.0
28	79806.0
29	95633.0
30	110255.0
31	124616.0
32	139599.0
33	154332.0
34	167170.0
35	179097.5
36	194957.0
37	211186.5
38	225176.5
39	236993.5
40	246674.0
41	258916.5
42	271876.0
43	285290.0
44	300996.0
45	317669.5
46	332470.0
47	343266.5
48	349211.0
49	352123.0
50	353596.5
51	353685.5
52	350407.5
53	345501.5
54	338382.0
55	323045.0
56	302685.0
57	277817.5
58	250802.0
59	222185.0
60	189595.5
61	157454.5
62	127883.5
63	101204.5
64	77536.5
65	58756.5
66	45162.5
67	34967.0
68	27033.0
69	19856.0
70	14258.0
71	10438.0
72	7653.0
73	5500.5
74	3879.0
75	2735.5
76	1838.0
77	1153.5
78	749.0
79	501.0
80	330.5
81	223.5
82	160.5
83	125.0
84	92.0
85	66.0
86	53.5
87	46.5
88	32.5
89	20.0
90	14.0
91	9.5
92	5.5
93	3.0
94	3.0
95	3.0
96	3.0
97	3.0
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.74377016312098E-4
3	0.004907467459069044
4	3.7366503495449565E-5
5	1.2455501165149858E-5
6	0.0
7	0.0
8	5.355865501014439E-4
9	0.0027402102563329686
10-11	8.843405827256397E-4
12-13	2.9270427738102166E-4
14-15	1.2455501165149858E-4
16-17	0.0017624534148687045
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	6.994807229982642E-4
32-33	8.076654336725112E-4
34-35	6.908236388796047E-5
36-37	8.76881320484856E-4
38-39	5.589292085035236E-4
40-41	2.5604596537170353E-5
42-43	1.3550243917295505E-4
44-45	0.0011527227376188134
46-47	0.0
48-49	5.123012512326001E-4
50-51	2.6887349065345332E-5
52-53	1.357074906463612E-5
54-55	6.574279773954347E-4
56-57	0.0010374534529217455
58-59	1.048212755323663E-4
60-61	1.8362064314118986E-4
62-63	1.4270919419681623E-5
64-65	0.0
66-67	0.0
68-69	2.209469166194945E-5
70-71	6.034679290321716E-4
72-73	0.0
74-75	0.0
76-77	7.703832032425737E-6
78-79	3.116761765561388E-4
80-81	0.0
82-83	0.0
84-85	1.6154689552907658E-5
86-87	3.922960573347226E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	147.0
20-21	529.0
22-23	1127.0
24-25	2956.0
26-27	4384.0
28-29	9130.0
30-31	19014.0
32-33	22892.0
34-35	31328.0
36-37	49392.0
38-39	61572.0
40-41	61146.0
42-43	68018.0
44-45	80766.0
46-47	81321.0
48-49	78291.0
50-51	71175.0
52-53	66634.0
54-55	71304.0
56-57	73838.0
58-59	74938.0
60-61	73865.0
62-63	69851.0
64-65	71013.0
66-67	75820.0
68-69	77249.0
70-71	77338.0
72-73	72567.0
74-75	73168.0
76-77	71730.0
78-79	76604.0
80-81	78018.0
82-83	73258.0
84-85	71936.0
86-87	74788.0
88-89	80781.0
90-91	86984.0
92-93	85384.0
94-95	93855.0
96-97	144450.0
98-99	670790.0
100-101	4899230.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.19238542458855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.53207220236384	71.06937743267238
2	8.02646011985215	13.033748872922502
3	2.3138983910526774	5.63612789999053
4	0.9360260754439181	3.0399275953963043
5	0.48301769734222755	1.9608679524753698
6	0.23967278149893612	1.1675762910746892
7	0.14702681078742239	0.8356220242440318
8	0.08542013950769049	0.5548371911944424
9	0.06122255344006408	0.4473724640024838
>10	0.17237624055177833	2.0627983089264936
>50	0.00230723329193366	0.12376487954616497
>100	4.717612687418687E-4	0.05545559011714225
>500	2.7993598619599827E-5	0.012523497437476225
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.4911002330299713E-5	0.0	0.0	1.2455501165149857E-5
3	0.0	2.4911002330299713E-5	0.0	0.0	1.2455501165149857E-5
4	0.0	4.9822004660599426E-5	0.0	1.2455501165149857E-5	1.2455501165149857E-5
5	0.0	7.473300699089914E-5	0.0	1.2455501165149857E-5	1.2455501165149857E-5
6	0.0	7.473300699089914E-5	0.0	1.2455501165149857E-5	1.2455501165149857E-5
7	0.0	7.473300699089914E-5	0.0	1.2455501165149857E-5	3.736650349544957E-5
8	0.0	8.7188508156049E-5	0.0	1.2455501165149857E-5	3.736650349544957E-5
9	0.0	2.2419902097269741E-4	0.0	1.2455501165149857E-5	3.736650349544957E-5
10-11	0.0	2.2419902097269741E-4	0.0	1.2455501165149857E-5	3.736650349544957E-5
12-13	0.0	2.3042677155527236E-4	0.0	3.736650349544957E-5	3.736650349544957E-5
14-15	0.0	3.6743728437192073E-4	0.0	3.736650349544957E-5	5.604975524317436E-5
16-17	0.0	3.8612053611964553E-4	0.0	5.604975524317436E-5	8.7188508156049E-5
18-19	1.2455501165149857E-5	3.985760372847954E-4	0.0	7.473300699089914E-5	1.0587175990377378E-4
20-21	1.2455501165149857E-5	4.234870396150951E-4	0.0	7.473300699089914E-5	1.1832726106892363E-4
22-23	1.2455501165149857E-5	4.670812936931196E-4	0.0	1.0587175990377378E-4	1.5569376456437323E-4
24-25	1.2455501165149857E-5	5.23131048936294E-4	0.0	1.2455501165149858E-4	1.8683251747724785E-4
26-27	1.2455501165149857E-5	5.729530535968934E-4	0.0	2.677932750507219E-4	1.8683251747724785E-4
28-29	1.2455501165149857E-5	5.978640559271931E-4	0.0	4.919922960234193E-4	1.8683251747724785E-4
30-31	1.2455501165149857E-5	6.97508065248392E-4	0.0	0.0017624534148687047	1.8683251747724785E-4
32-33	1.2455501165149857E-5	6.97508065248392E-4	0.0	0.004054265629256279	1.8683251747724785E-4
34-35	1.2455501165149857E-5	8.469740792301902E-4	0.0	0.006862981141997571	1.8683251747724785E-4
36-37	1.2455501165149857E-5	0.0010151233449597133	0.0	0.011153901293391697	1.8683251747724785E-4
38-39	1.2455501165149857E-5	0.0010960841025331874	0.0	0.01786741642140747	1.9306026805982276E-4
40-41	1.2455501165149857E-5	0.001201955862436961	0.0	0.02563964914846098	2.0551576922497265E-4
42-43	1.2455501165149857E-5	0.0012642333682627103	0.0	0.03287006757483047	2.2419902097269741E-4
44-45	1.2455501165149857E-5	0.0014136993822445086	0.0	0.040592478297223386	2.61565524468147E-4
46-47	1.2455501165149857E-5	0.0015195711421482825	0.0	0.048987486082534386	2.61565524468147E-4
48-49	1.2455501165149857E-5	0.0015257988927308574	0.0	0.05761292063940066	2.61565524468147E-4
50-51	1.2455501165149857E-5	0.0016877204078778055	0.0	0.06628194945034496	2.61565524468147E-4
52-53	1.2455501165149857E-5	0.00173131466195583	0.0	0.07444653046410069	2.9270427738102166E-4
54-55	1.2455501165149857E-5	0.0017499979137035548	0.0	0.08270452773659505	3.238430302938963E-4
56-57	1.2455501165149857E-5	0.0017562256642861297	0.0	0.0898228466524782	3.549817832067709E-4
58-59	1.2455501165149857E-5	0.0017811366666164296	0.0	0.09657995603457198	3.8612053611964553E-4
60-61	1.2455501165149857E-5	0.001830958671277029	0.0	0.10296340038171128	3.985760372847954E-4
62-63	1.2455501165149857E-5	0.0018558696736073286	0.0	0.1093219337265203	3.985760372847954E-4
64-65	1.2455501165149857E-5	0.0020177911887542766	0.0	0.11612263736269211	3.985760372847954E-4
66-67	1.2455501165149857E-5	0.0020364744405020016	0.0	0.12293579650002909	3.985760372847954E-4
68-69	1.2455501165149857E-5	0.002073840943997451	0.0	0.12974272788678348	4.110315384499453E-4
70-71	1.2455501165149857E-5	0.0021049796969103258	0.0	0.13602030047401903	4.234870396150951E-4
72-73	1.2455501165149857E-5	0.002142346200405775	0.0	0.14150072098668495	4.608535431105447E-4
74-75	1.2455501165149857E-5	0.002192168205066375	0.0	0.1456857693781753	4.608535431105447E-4
76-77	1.2455501165149857E-5	0.0022793567132224236	0.0	0.14915462645266953	4.608535431105447E-4
78-79	1.2455501165149857E-5	0.0023042677155527234	0.0	0.15226227399337444	4.608535431105447E-4
80-81	2.4911002330299713E-5	0.0023104954661352984	0.0	0.15388771689542646	4.857645454408444E-4
82-83	2.4911002330299713E-5	0.0023167232167178734	0.0	0.1543797091914499	5.106755477711441E-4
84-85	2.4911002330299713E-5	0.002335406468465598	0.0	0.15473469097465667	5.106755477711441E-4
86-87	2.4911002330299713E-5	0.0023665452213784727	0.0	0.15493397899329905	5.106755477711441E-4
88-89	2.4911002330299713E-5	0.002416367226039072	0.0	0.15500871200028996	5.169032983537191E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10710	0.0	40.150127	1
GTATCAA	17995	0.0	34.036488	1
ATCAACG	21130	0.0	28.207127	3
TCAACGC	21355	0.0	28.047518	4
CAACGCA	21545	0.0	27.682543	5
TATCAAC	22310	0.0	27.056025	2
AACGCAG	22240	0.0	26.8936	6
GTACATG	26590	0.0	23.669907	1
TACATGG	26580	0.0	22.995605	2
ACGCAGA	26175	0.0	22.80244	7
CGCAGAG	26470	0.0	22.564274	8
ACATGGG	27505	0.0	21.65403	3
CATGGGG	21710	0.0	19.164354	4
GCAGAGT	31065	0.0	19.063377	9
GAGTACT	15525	0.0	18.488255	12-13
AGAGTAC	26685	0.0	17.67351	10-11
CAGAGTA	29640	0.0	17.094381	10-11
GAGTACA	19580	0.0	16.481976	1
AGTACTT	16965	0.0	16.221785	12-13
GTACTTT	17975	0.0	15.980447	14-15
>>END_MODULE