FastQCFastQC Report
Fri 27 May 2016
SRR2139999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6981835
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66700.040.677211
GTATCAA108800.031.533771
TCAACGC124950.026.2632714
ATCAACG125200.026.1447433
CAACGCA127900.025.5928215
AACGCAG133100.024.7178086
TATCAAC136400.024.4536692
GTACATG191250.022.4511281
TACATGG184250.022.2910272
CTTATAC50600.022.0546931
ACATGGG186350.021.3724143
ACGCAGA157000.020.8229087
CGCAGAG159850.020.4256978
CATGGGG132850.019.3927174
TATACAC81250.017.6670673
TATACCG4550.016.4124265
AGAGTAC174200.016.24196610-11
GCAGAGT201800.016.1795069
GAGTACA131750.015.9645471
GAGTACT109100.015.60939812-13
Produced by FastQC (version 0.11.5)