##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139999_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6981835
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73377400067461	34.0	31.0	34.0	31.0	34.0
2	32.91609999376955	34.0	31.0	34.0	31.0	34.0
3	33.014666344879245	34.0	33.0	34.0	31.0	34.0
4	36.39980449265845	37.0	37.0	37.0	35.0	37.0
5	36.24463926747052	37.0	37.0	37.0	35.0	37.0
6	36.297091667162	37.0	37.0	37.0	35.0	37.0
7	36.3000456183797	37.0	37.0	37.0	35.0	37.0
8	36.285143089173545	37.0	37.0	37.0	35.0	37.0
9	38.18626177788504	39.0	39.0	39.0	37.0	39.0
10-11	38.08455040830957	39.0	38.0	39.0	37.0	39.0
12-13	38.12106258598205	39.0	38.0	39.0	37.0	39.0
14-15	39.55890664846706	41.0	39.0	41.0	37.0	41.0
16-17	39.539293180088045	41.0	39.0	41.0	37.0	41.0
18-19	39.58718746575936	41.0	39.5	41.0	37.0	41.0
20-21	39.603613863332086	41.0	39.5	41.0	37.0	41.0
22-23	39.53998902669029	41.0	39.0	41.0	37.0	41.0
24-25	39.49933420368923	41.0	39.0	41.0	37.0	41.0
26-27	39.46102102762967	41.0	39.0	41.0	37.0	41.0
28-29	39.36642614881967	40.5	39.0	41.0	36.5	41.0
30-31	39.28022789919912	40.0	39.0	41.0	36.0	41.0
32-33	39.17973559437069	40.0	39.0	41.0	36.0	41.0
34-35	39.111187590115726	40.0	39.0	41.0	35.5	41.0
36-37	39.11548267580639	40.0	39.0	41.0	35.0	41.0
38-39	39.104478170608516	40.0	39.0	41.0	35.0	41.0
40-41	39.072561719520884	40.0	39.0	41.0	35.0	41.0
42-43	39.04064343115472	40.0	39.0	41.0	35.0	41.0
44-45	39.0070674079306	40.0	39.0	41.0	35.0	41.0
46-47	38.94150208374157	40.0	38.0	41.0	35.0	41.0
48-49	38.9145236895899	40.0	38.0	41.0	35.0	41.0
50-51	38.8159207727156	40.0	38.0	41.0	35.0	41.0
52-53	38.65409335528686	40.0	38.0	41.0	35.0	41.0
54-55	38.51902824850856	40.0	38.0	41.0	34.5	41.0
56-57	38.36260470495941	40.0	37.5	41.0	34.0	41.0
58-59	38.181739810705565	40.0	37.0	41.0	34.0	41.0
60-61	38.05699802274955	40.0	37.0	41.0	34.0	41.0
62-63	37.89142836198475	39.0	36.0	41.0	34.0	41.0
64-65	37.65098938013834	39.0	36.0	41.0	33.5	41.0
66-67	37.38817301517484	39.0	35.0	40.5	33.0	41.0
68-69	37.135725106995366	38.0	35.0	40.0	33.0	41.0
70-71	36.84068506593209	37.5	35.0	40.0	33.0	41.0
72-73	36.487249484015656	37.0	35.0	39.0	33.0	41.0
74-75	36.10357452140455	36.5	35.0	39.0	32.0	40.5
76-77	34.16523760735825	34.5	33.0	36.5	29.5	39.0
78-79	35.229579270141485	35.0	34.0	37.0	31.5	39.0
80-81	35.14728055015651	35.0	34.0	37.0	32.0	39.0
82-83	34.86337017822029	35.0	34.0	37.0	32.0	39.0
84-85	34.614790605091805	35.0	34.0	36.0	31.5	37.5
86-87	34.40250861806708	35.0	34.0	36.0	31.0	37.0
88-89	34.242033595806205	35.0	34.0	35.5	31.0	37.0
90-91	34.09974312293724	35.0	34.0	35.0	31.0	36.0
92-93	34.010620672125754	35.0	34.0	35.0	31.0	36.0
94-95	33.927911961567105	35.0	34.0	35.0	31.0	36.0
96-97	33.88967197281566	35.0	34.0	35.0	31.0	36.0
98-99	33.91015685845501	35.0	34.0	35.0	31.0	35.5
100-101	32.666116505848805	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	21.0
21	86.0
22	344.0
23	1058.0
24	2903.0
25	6385.0
26	12038.0
27	19959.0
28	30048.0
29	42580.0
30	58862.0
31	78168.0
32	105425.0
33	147493.0
34	226804.0
35	369504.0
36	733917.0
37	1624254.0
38	2358057.0
39	1138910.0
40	25009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2461859955155	20.037096264807175	13.09384996924161	24.622867770435708
2	14.775077207684303	21.32159560733322	40.99559742176035	22.90772976322213
3	18.457351572011746	26.012619064670915	30.273551529041036	25.256477834276303
4	12.22537469039282	17.82003889810567	39.17366375668503	30.78092265481648
5	12.944535776702798	38.01687350343764	36.03369830906893	13.00489241079063
6	29.90407249670037	38.20326604682007	18.89321360358702	12.999447852892542
7	26.476721377689387	32.388877709083644	23.40366107191018	17.73073984131679
8	24.410060985871656	36.79013993707336	21.613783073402047	17.186016003652938
9	25.545259644312186	16.394843724128528	21.20005534525758	36.8598412863017
10-11	24.12216929788798	26.710717575148024	29.276155668537086	19.890957458426914
12-13	24.774832776195176	24.1832670635169	29.31423523209093	21.727664928196997
14-15	21.81519002444275	25.731049852219883	26.66728780316838	25.786472320168986
16-17	21.728979726532195	28.9019808253993	27.356190494742282	22.012848953326223
18-19	21.706786253184156	28.233422875218334	28.7100382635797	21.349752608017805
20-21	22.47642094701512	27.48805336337434	28.54440639584162	21.491119293768914
22-23	22.093955351497446	27.59047462705296	28.36026923293995	21.95530078850964
24-25	21.93933526643665	27.614505280979696	28.55720655992404	21.88895289265961
26-27	21.958533719006404	27.824243899638912	28.386880073452076	21.83034230790261
28-29	21.724682515158474	27.878428714738117	28.430840972778533	21.966047797324876
30-31	22.146626149668155	27.676834439381697	28.448196419191625	21.728342991758527
32-33	21.81836780636273	27.94139735081448	28.29250898219769	21.947725860625106
34-35	21.91561510985211	27.959301441530798	28.466778936524246	21.658304512092847
36-37	22.049300087015204	27.811184053120208	28.302137677700877	21.837378182163707
38-39	21.78616072452844	27.871285383240853	28.44952535488155	21.89302853734916
40-41	22.030527651554884	27.740939581626655	28.377906334188836	21.850626432629625
42-43	22.023541840980446	27.766378328534664	28.276471954816675	21.933607875668216
44-45	21.979047558412486	27.982744764114926	28.112846997751596	21.925360679720995
46-47	22.109377725258593	27.820454916527748	27.823286800117337	22.246880558096322
48-49	22.002854314138276	27.8804632391214	27.968656603458847	22.14802584328147
50-51	21.94717546424733	28.050395516233035	27.885284671715276	22.117144347804356
52-53	22.0904642311576	27.98847268435401	27.86763605408884	22.053427030399554
54-55	22.07010453109554	27.93123973303313	27.835266454291553	22.163389281579775
56-57	22.192498323842848	28.07339812456078	27.642671146644883	22.091432404951494
58-59	22.007309516716138	28.171815736098903	27.811807938627197	22.00906680855777
60-61	22.155667909232683	28.234128732818515	27.649953057237898	21.960250300710907
62-63	22.04239373233563	28.28892431011741	27.63190882532222	22.036773132224745
64-65	22.034633542954865	28.1774930716044	27.67283984997992	22.115033535460814
66-67	22.13567411015725	28.17332783309731	27.53401554002688	22.156982516718557
68-69	22.118232873824116	28.13403504108584	27.623167071761234	22.124565013328805
70-71	22.097063728039284	27.85489094071049	27.717336619881866	22.330708711368356
72-73	22.13624885866033	27.918013562705994	27.70518354436873	22.240554034264946
74-75	22.016213389357418	28.06848806925903	27.64028191028714	22.275016631096417
76-77	22.08893890947309	28.026901465733683	27.577812705279147	22.30634691951408
78-79	22.12694906767106	28.035874947307494	27.51882218966194	22.318353795359506
80-81	22.098726412940582	28.005339279283554	27.570615737560377	22.325318570215487
82-83	22.19417012839027	27.94107628764384	27.665357734347708	22.199395849618185
84-85	22.297982118551797	27.934222674692457	27.57728652896582	22.19050867778993
86-87	22.09036988103368	27.986562574833346	27.636338863029238	22.28672868110374
88-89	22.195865510225172	27.967481554418296	27.58954942372562	22.247103511630907
90-91	22.2316860975304	27.960416196565124	27.597542935940826	22.210354769963644
92-93	22.23456060024806	27.948408697185073	27.613999501191877	22.203031201374994
94-95	22.07081216347239	28.043231509068985	27.686837275007846	22.19911905245078
96-97	22.102920565631667	28.044641519173908	27.715250300573828	22.137187614620593
98-99	22.375573653703174	28.56870711864325	27.746363853639078	21.309355374014498
100-101	26.3946762928649	35.69923082582675	28.324693827951663	9.581399053356684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	883.0
1	838.5
2	1235.5
3	1811.5
4	2533.0
5	4358.0
6	5942.5
7	6850.0
8	7272.0
9	6987.0
10	6335.5
11	5679.0
12	6101.5
13	8059.0
14	12553.5
15	18543.5
16	23377.5
17	26483.0
18	27722.0
19	27316.5
20	26276.5
21	25707.5
22	26896.5
23	30762.0
24	37825.0
25	48088.0
26	61891.5
27	77528.0
28	93817.0
29	111046.0
30	126885.0
31	141940.0
32	157637.5
33	173996.5
34	191620.0
35	208738.0
36	226275.5
37	241636.5
38	252163.0
39	262121.5
40	274389.5
41	285128.0
42	292235.0
43	299185.5
44	306027.0
45	312040.0
46	317186.0
47	320593.5
48	318148.0
49	311769.0
50	304960.0
51	293666.5
52	275737.0
53	257344.0
54	240046.5
55	219577.5
56	198834.5
57	177428.0
58	155676.0
59	135090.5
60	114267.0
61	93680.0
62	74456.0
63	57911.0
64	43862.0
65	32991.0
66	24903.5
67	19090.5
68	14626.0
69	10511.5
70	7487.0
71	5413.0
72	3822.0
73	2655.0
74	1899.5
75	1311.5
76	860.5
77	581.5
78	380.0
79	257.5
80	188.5
81	138.5
82	104.5
83	75.0
84	52.0
85	36.5
86	27.0
87	20.5
88	13.5
89	10.0
90	7.5
91	5.0
92	5.0
93	3.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.4348899680384886E-4
3	0.004798165525252316
4	5.729152865972914E-5
5	1.4322882164932286E-5
6	0.0
7	0.0
8	7.734356369063434E-4
9	0.002964836608140983
10-11	9.238258996381323E-4
12-13	3.437491719583748E-4
14-15	1.360673805668567E-4
16-17	0.0014179653343282961
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	6.679844395538726E-4
32-33	6.699458957457571E-4
34-35	3.61523175226234E-5
36-37	7.051897018457941E-4
38-39	6.963695397114625E-4
40-41	5.178288089027496E-5
42-43	4.4791529026030595E-5
44-45	8.825782448988674E-4
46-47	0.0
48-49	4.7108317699421287E-4
50-51	7.810351543141922E-6
52-53	2.368106904555109E-5
54-55	7.897987991548036E-4
56-57	9.122409659485474E-4
58-59	7.356099972341064E-5
60-61	8.275491098674886E-5
62-63	0.0
64-65	2.5432298204886663E-5
66-67	0.0
68-69	8.697993068569324E-6
70-71	2.9075578971871665E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	3.145811509265988E-4
80-81	0.0
82-83	9.501310420733227E-6
84-85	3.84898914479214E-5
86-87	2.1441824286004308E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	4.1372619393622204E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	189.0
20-21	474.0
22-23	884.0
24-25	2469.0
26-27	3761.0
28-29	8436.0
30-31	19260.0
32-33	24013.0
34-35	32770.0
36-37	52699.0
38-39	62889.0
40-41	60520.0
42-43	66455.0
44-45	76953.0
46-47	76755.0
48-49	74001.0
50-51	68858.0
52-53	65650.0
54-55	72058.0
56-57	76393.0
58-59	75583.0
60-61	74127.0
62-63	70972.0
64-65	72107.0
66-67	76575.0
68-69	73923.0
70-71	71697.0
72-73	66357.0
74-75	66737.0
76-77	66737.0
78-79	70560.0
80-81	71680.0
82-83	66699.0
84-85	65768.0
86-87	68088.0
88-89	73200.0
90-91	77446.0
92-93	74517.0
94-95	81357.0
96-97	122806.0
98-99	554399.0
100-101	4095013.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.72277696565737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.82848535802177	79.6772696319022
2	6.743690606116029	11.831505339314303
3	1.4939996512200258	3.9317339457223275
4	0.48598471377992103	1.7052771462253937
5	0.19680667981753405	0.8632214239492542
6	0.09989880913113841	0.5258040571527378
7	0.049513439220778	0.3040419469896858
8	0.030383584558765917	0.21322659293326512
9	0.01909150524932859	0.15072838712329756
>10	0.05107392978240579	0.6558961395846746
>50	7.615511873318013E-4	0.0464049342632248
>100	2.422439352997269E-4	0.03951320913777058
>500	4.528547168983626E-5	0.029238015673736552
>1k	2.2642508008286012E-5	0.02613923002819444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.864576432986457E-5	0.0	0.0	0.0
3	0.0	2.864576432986457E-5	0.0	0.0	0.0
4	0.0	5.729152865972914E-5	0.0	0.0	0.0
5	0.0	5.729152865972914E-5	0.0	0.0	0.0
6	0.0	5.729152865972914E-5	0.0	0.0	0.0
7	0.0	5.729152865972914E-5	0.0	0.0	0.0
8	0.0	7.161441082466142E-5	0.0	0.0	0.0
9	0.0	1.5755170381425514E-4	0.0	1.4322882164932286E-5	0.0
10-11	0.0	1.5755170381425514E-4	0.0	7.161441082466142E-5	0.0
12-13	0.0	1.5755170381425514E-4	0.0	8.593729298959371E-5	0.0
14-15	0.0	3.079419665460441E-4	0.0	1.1458305731945827E-4	0.0
16-17	0.0	3.151034076285103E-4	0.0	1.50390262731789E-4	0.0
18-19	0.0	3.437491719583748E-4	0.0	2.0768179139151813E-4	0.0
20-21	0.0	3.723949362882394E-4	0.0	2.578118789687811E-4	0.0
22-23	0.0	4.01040700618104E-4	0.0	3.151034076285103E-4	1.4322882164932286E-5
24-25	0.0	4.082021417005701E-4	0.0	3.9387925953563786E-4	2.864576432986457E-5
26-27	0.0	4.1536358278303627E-4	0.0	4.5117078819536697E-4	2.864576432986457E-5
28-29	0.0	4.1536358278303627E-4	0.0	7.805970779888095E-4	2.864576432986457E-5
30-31	0.0	4.5833222927783314E-4	0.0	0.0020768179139151813	2.864576432986457E-5
32-33	0.0	4.6549367036029926E-4	0.0	0.004361317619221881	2.864576432986457E-5
34-35	0.0	5.58592404432359E-4	0.0	0.007190086846796007	2.864576432986457E-5
36-37	0.0	6.445296974219528E-4	0.0	0.011336561233543905	3.580720541233072E-5
38-39	0.0	6.588525795868851E-4	0.0	0.01791792558833029	4.296864649479686E-5
40-41	0.0	7.01821226081682E-4	0.0	0.02513665819945616	4.296864649479686E-5
42-43	0.0	7.233055493290803E-4	0.0	0.031574793732593226	4.296864649479686E-5
44-45	0.0	7.877585190712757E-4	0.0	0.03877920346155416	4.296864649479686E-5
46-47	0.0	8.307271655660725E-4	0.0	0.047501838699997925	4.296864649479686E-5
48-49	0.0	8.307271655660725E-4	0.0	0.055372262449628215	5.729152865972914E-5
50-51	0.0	9.59633105050463E-4	0.0	0.06373682563394867	5.729152865972914E-5
52-53	0.0	9.739559872153954E-4	0.0	0.07117040147754852	7.161441082466144E-5
54-55	0.0	0.00100260175154526	0.0	0.0782817124724374	8.593729298959371E-5
56-57	0.0	0.001059893280204989	0.0	0.08582987137335671	9.309873407205985E-5
58-59	0.0	0.001059893280204989	0.0	0.09360719638891495	1.00260175154526E-4
60-61	0.0	0.0010742161623699213	0.0	0.10084741332328821	1.00260175154526E-4
62-63	0.0	0.001102861926699786	0.0	0.1075791679408064	1.00260175154526E-4
64-65	0.0	0.0012317678661841766	0.0	0.11391704329878893	1.00260175154526E-4
66-67	0.0	0.0012389293072666428	0.0	0.12036234027300846	1.0742161623699214E-4
68-69	0.0	0.0012675750715965073	0.0	0.12710841777269155	1.1458305731945829E-4
70-71	0.0	0.0012747365126789733	0.0	0.13359668339340589	1.1458305731945829E-4
72-73	0.0	0.0012747365126789733	0.0	0.13908234726257496	1.1458305731945829E-4
74-75	0.0	0.0012890593948439058	0.0	0.14405238737380646	1.1458305731945829E-4
76-77	0.0	0.0013391894824211687	0.0	0.14834209058220366	1.1458305731945829E-4
78-79	0.0	0.001360673805668567	0.0	0.15219494588457044	1.1458305731945829E-4
80-81	0.0	0.001360673805668567	0.0	0.15379910868704288	1.1458305731945829E-4
82-83	0.0	0.001360673805668567	0.0	0.15452957567745443	1.1458305731945829E-4
84-85	0.0	0.0013678352467510332	0.0	0.1550881680818868	1.1458305731945829E-4
86-87	0.0	0.0014179653343282961	0.0	0.15531017275544323	1.1458305731945829E-4
88-89	0.0	0.0015182255094828223	0.0	0.15541759437168023	1.1458305731945829E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6670	0.0	40.67721	1
GTATCAA	10880	0.0	31.53377	1
TCAACGC	12495	0.0	26.263271	4
ATCAACG	12520	0.0	26.144743	3
CAACGCA	12790	0.0	25.592821	5
AACGCAG	13310	0.0	24.717808	6
TATCAAC	13640	0.0	24.453669	2
GTACATG	19125	0.0	22.451128	1
TACATGG	18425	0.0	22.291027	2
CTTATAC	5060	0.0	22.054693	1
ACATGGG	18635	0.0	21.372414	3
ACGCAGA	15700	0.0	20.822908	7
CGCAGAG	15985	0.0	20.425697	8
CATGGGG	13285	0.0	19.392717	4
TATACAC	8125	0.0	17.667067	3
TATACCG	455	0.0	16.412426	5
AGAGTAC	17420	0.0	16.241966	10-11
GCAGAGT	20180	0.0	16.179506	9
GAGTACA	13175	0.0	15.964547	1
GAGTACT	10910	0.0	15.609398	12-13
>>END_MODULE