FastQCFastQC Report
Fri 27 May 2016
SRR2139998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10407600
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107350.045.72771
GTATCAA182200.033.8898471
TCAACGC217100.027.6146644
ATCAACG217050.027.5632823
CAACGCA221200.027.121845
AACGCAG230650.026.0470986
TATCAAC233450.026.020782
ACGCAGA270250.022.2303927
GTACATG301350.022.0706651
TACATGG293900.021.8344062
CGCAGAG275800.021.7678978
ACATGGG299500.020.7843843
CATGGGG221200.018.0181834
GCAGAGT336500.017.704449
GAGTACT172950.017.13884712-13
GTGGTAT57500.016.8563561
AGAGTAC289700.016.80061310-11
CAGAGTA319500.016.7896610-11
TGGTATC56300.015.2123142
TATACTG58450.015.1530885
Produced by FastQC (version 0.11.5)