##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139998_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10407600
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84439822821784	31.0	31.0	34.0	30.0	34.0
2	32.03365261923979	33.0	31.0	34.0	30.0	34.0
3	32.097450228679044	33.0	31.0	34.0	30.0	34.0
4	35.77976152042738	37.0	35.0	37.0	35.0	37.0
5	35.54398756677812	37.0	35.0	37.0	33.0	37.0
6	35.57174507090972	37.0	35.0	37.0	33.0	37.0
7	35.533665398362736	37.0	35.0	37.0	33.0	37.0
8	35.52609343172297	37.0	35.0	37.0	33.0	37.0
9	37.30543276067489	39.0	37.0	39.0	34.0	39.0
10-11	37.16099715592452	39.0	37.0	39.0	33.0	39.0
12-13	37.16482450324763	39.0	37.0	39.0	33.0	39.0
14-15	38.295941811752954	40.0	38.0	41.0	33.5	41.0
16-17	38.22886342672662	40.0	38.0	41.0	33.0	41.0
18-19	38.290260050347825	40.0	38.0	41.0	33.5	41.0
20-21	38.328223844879204	40.0	38.0	41.0	34.0	41.0
22-23	38.31306132277517	40.0	38.0	41.0	34.0	41.0
24-25	38.267700939490595	40.0	38.0	41.0	34.0	41.0
26-27	38.15131537531193	40.0	38.0	41.0	33.5	41.0
28-29	37.985597836817	40.0	37.0	41.0	33.0	41.0
30-31	37.889925873341646	40.0	37.0	41.0	33.0	41.0
32-33	37.80244916638056	40.0	37.0	41.0	33.0	41.0
34-35	37.7350979317014	40.0	37.0	41.0	33.0	41.0
36-37	37.69572330123931	39.5	37.0	41.0	33.0	41.0
38-39	37.606936778629546	39.0	37.0	41.0	32.5	41.0
40-41	37.59154548432501	39.0	37.0	41.0	32.5	41.0
42-43	37.53873595667346	39.0	37.0	41.0	32.0	41.0
44-45	37.466175737903356	39.0	36.5	41.0	32.0	41.0
46-47	37.40753865798814	39.0	36.0	40.5	32.0	41.0
48-49	37.414445995594185	39.0	36.0	40.0	32.0	41.0
50-51	37.30774793766422	39.0	36.0	40.0	31.5	41.0
52-53	37.11933880713368	39.0	36.0	40.0	31.0	41.0
54-55	36.924947318509496	39.0	35.0	40.0	31.0	41.0
56-57	36.80312579914141	39.0	35.0	40.0	31.0	41.0
58-59	36.60573419695115	38.0	35.0	40.0	30.5	41.0
60-61	36.419703173633096	38.0	35.0	40.0	30.0	41.0
62-63	36.2679791042614	38.0	34.5	40.0	30.0	41.0
64-65	36.002638585754795	37.5	34.0	40.0	30.0	41.0
66-67	35.853255009086396	37.0	34.0	40.0	30.0	41.0
68-69	35.57452151630012	36.5	34.0	39.0	29.5	41.0
70-71	35.25376796420278	36.0	34.0	39.0	29.0	40.5
72-73	34.95558455429774	36.0	34.0	39.0	29.0	40.0
74-75	34.598889633417166	35.0	33.0	38.0	29.0	40.0
76-77	32.47251621113696	33.5	30.5	35.5	26.5	38.5
78-79	33.74614612946934	35.0	32.5	36.0	28.5	39.0
80-81	33.75086907704066	35.0	33.0	36.0	29.0	39.0
82-83	33.58554908746186	35.0	33.0	36.0	29.0	37.5
84-85	33.395325250680074	35.0	33.0	35.5	29.0	37.0
86-87	33.16467359620016	35.0	32.5	35.0	29.0	37.0
88-89	33.01643106771564	34.5	32.0	35.0	29.0	36.0
90-91	32.86113179733339	34.0	32.0	35.0	28.5	36.0
92-93	32.830700349386014	34.0	32.0	35.0	29.0	36.0
94-95	32.799432574882786	34.0	32.0	35.0	29.0	35.0
96-97	32.8284604865098	34.0	32.0	35.0	29.0	35.0
98-99	32.96935990040614	34.0	32.0	35.0	29.0	35.0
100-101	31.766118497160143	33.0	30.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	12.0
20	66.0
21	370.0
22	1515.0
23	5289.0
24	14225.0
25	30839.0
26	56638.0
27	91175.0
28	134544.0
29	184907.0
30	242986.0
31	311763.0
32	398520.0
33	522057.0
34	713649.0
35	1013432.0
36	1570177.0
37	2310875.0
38	2127390.0
39	670799.0
40	6367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34671778315846	20.33182482032361	14.004477497213575	24.316979899304357
2	14.84681793505676	21.249362241834053	41.18705170765454	22.71676811545465
3	18.5672461801879	25.927468249103057	30.037376604359515	25.46790896634953
4	12.813406819382644	17.909164255848058	38.42241615957725	30.855012765192047
5	13.318277028325454	37.79143126177025	35.537309273992086	13.352982435912217
6	30.40677005265383	37.75036511779854	18.61615550174872	13.226709327798917
7	26.8028389641069	32.238588362214955	23.151410810504903	17.807161863173246
8	24.352506749230336	37.179440984914415	21.053985867668484	17.41406639818677
9	25.600119143702678	16.66749298589492	21.103760713324878	36.62862715707752
10-11	24.407130285602005	26.70913091927492	28.825886715712663	20.05785207941041
12-13	25.0699467569677	24.09790326434854	29.000652314341913	21.83149766434185
14-15	22.174843383681157	25.53862081555786	26.42959952342519	25.856936277335794
16-17	22.015008263192282	28.618014720012297	27.095478304316078	22.271498712479342
18-19	22.012339010664412	28.027672767311802	28.534121901092963	21.42586632093083
20-21	22.766631469822332	27.2187296024294	28.374181562868646	21.640457364879623
22-23	22.475618457039314	27.097681417644502	28.258738390259115	22.167961735057062
24-25	22.23841454467648	27.21560402027352	28.335216447781146	22.210764987268853
26-27	22.234503897336285	27.39900037133516	28.198777349065097	22.16771838226346
28-29	22.17884420404496	27.5251135725883	28.208281272012993	22.08776095135375
30-31	22.47227784230961	27.354734236102203	28.105819843728785	22.067168077859407
32-33	22.226502555065586	27.53169836644235	28.048103639951993	22.19369543854007
34-35	22.324437318427652	27.52723597361176	28.094232328444736	22.054094379515856
36-37	22.378900105475505	27.41739542489344	28.07841617308603	22.12528829654503
38-39	22.197851534803753	27.587846593716524	28.121081661402552	22.09322021007717
40-41	22.446891445367935	27.40059679096869	27.995641715497783	22.15687004816559
42-43	22.31607130539746	27.514066182115656	27.851202683446154	22.318659829040733
44-45	22.38617591314789	27.626605105599012	27.70979251642648	22.277426464826615
46-47	22.44732413998952	27.536466179712626	27.530172740149837	22.486036940148015
48-49	22.37922966034859	27.63442092241085	27.5490841910501	22.437265226190465
50-51	22.36933991465394	27.73667428735851	27.466427015551975	22.427558782435575
52-53	22.563284405449867	27.60482634841192	27.320144552819198	22.511744693319017
54-55	22.478163006650792	27.514577137677364	27.396971189274705	22.61028866639714
56-57	22.49384112144271	27.629856559591055	27.373919599054258	22.502382719911978
58-59	22.467611937271947	27.709380959697956	27.356913301132234	22.466093801897866
60-61	22.538478968369475	27.622968295773624	27.378699862840293	22.459852873016608
62-63	22.541785606778515	27.690272712952357	27.3075019335652	22.460439746703926
64-65	22.49877527776252	27.66275353431857	27.295263996305096	22.54320719161382
66-67	22.55571997302563	27.713153339013758	27.147497893655142	22.583628794305465
68-69	22.480040272470585	27.701685232987376	27.270179716474	22.548094778068034
70-71	22.48426222385536	27.432436808081533	27.3531208229981	22.730180145065006
72-73	22.558747698472917	27.507128795624862	27.261318609525315	22.672804896376903
74-75	22.403233811159275	27.633356391915164	27.262698826634423	22.700710970291137
76-77	22.471554089680783	27.625024604787672	27.1893312916539	22.714090013877644
78-79	22.49252983741722	27.584803967009375	27.159542898583794	22.763123296989612
80-81	22.47479965846172	27.64679158681995	27.16758121816972	22.71082753654861
82-83	22.54617404221747	27.55226371029523	27.28254207008768	22.61902017739962
84-85	22.64607771618073	27.56309997324716	27.159929156536727	22.63089315403538
86-87	22.425483855951843	27.61515476927791	27.26779766154717	22.69156371322308
88-89	22.456492018615386	27.55337910229955	27.252041178698356	22.738087700386707
90-91	22.535693146300147	27.581821242448427	27.259106498797596	22.62337911245382
92-93	22.541715984299675	27.585041667647538	27.240612542471055	22.632629805581733
94-95	22.391456732822128	27.61128986685979	27.286353979553123	22.710899420764957
96-97	22.361315075686274	27.610757194473823	27.354608876396547	22.673318853443355
98-99	22.595519627936145	28.205310697505205	27.41885608034452	21.78031359421414
100-101	26.831120511560425	35.239872535530395	27.994660409419375	9.9343465434898
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2072.0
1	1892.5
2	2415.5
3	3364.0
4	4563.5
5	7348.0
6	9755.5
7	11371.5
8	12385.5
9	12250.0
10	11368.0
11	10239.5
12	10861.0
13	14203.5
14	21860.5
15	32113.0
16	40096.0
17	44395.5
18	45610.0
19	44293.5
20	41827.0
21	39789.0
22	40275.5
23	45601.5
24	55777.0
25	70830.0
26	90152.5
27	111032.5
28	132492.0
29	153951.5
30	173124.0
31	192506.5
32	215238.5
33	238302.5
34	261597.5
35	285641.5
36	311352.5
37	334093.5
38	351728.0
39	366208.5
40	381138.5
41	396959.5
42	408041.5
43	419535.0
44	432744.0
45	444679.5
46	452782.0
47	457245.5
48	459278.5
49	456700.5
50	450285.0
51	439468.5
52	422667.5
53	403688.5
54	382875.0
55	356161.0
56	327253.5
57	296711.0
58	264349.0
59	230618.0
60	194030.5
61	158301.0
62	125781.5
63	97551.5
64	73306.5
65	54340.5
66	40748.5
67	30707.0
68	22946.0
69	16216.5
70	11082.5
71	7577.5
72	5090.0
73	3355.5
74	2244.5
75	1556.5
76	1096.5
77	776.0
78	545.5
79	395.5
80	283.0
81	191.0
82	133.0
83	94.0
84	67.0
85	49.5
86	43.0
87	37.5
88	25.5
89	14.5
90	8.0
91	5.0
92	3.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.8041815596295016E-4
3	1.9216726238518005E-4
4	9.608363119259003E-6
5	0.0
6	0.0
7	9.608363119259003E-6
8	0.0011241784849533034
9	0.0
10-11	2.1618817018332758E-4
12-13	3.9394288788961913E-4
14-15	0.0
16-17	0.0
18-19	3.795303432107306E-4
20-21	0.0
22-23	0.0
24-25	1.5860141618568785E-4
26-27	0.0
28-29	4.522866893278336E-4
30-31	3.903054863218406E-4
32-33	0.001053295444975532
34-35	0.0012459385191880108
36-37	2.9229727977921035E-5
38-39	0.0016241045774185382
40-41	4.0064875420495704E-4
42-43	1.446379134474756E-4
44-45	2.870433323132384E-4
46-47	4.073422005959722E-5
48-49	0.0
50-51	0.0
52-53	3.1516324431775113E-5
54-55	3.554319336176223E-4
56-57	5.737261724013904E-4
58-59	4.82547843940991E-4
60-61	6.415953504188164E-4
62-63	1.275272575651387E-4
64-65	1.0649747245064528E-4
66-67	2.4381885189218175E-4
68-69	1.2054174214158246E-4
70-71	9.882515493168333E-5
72-73	0.0
74-75	1.1918631502730857E-5
76-77	7.542796469464377E-4
78-79	4.2774793523016326E-5
80-81	9.911141659452183E-5
82-83	0.0
84-85	3.820014174799264E-5
86-87	2.582484056550461E-5
88-89	3.2772635059470205E-5
90-91	9.996110180325162E-5
92-93	1.0176150522362158E-4
94-95	3.528364975733497E-4
96-97	7.79707585555364E-5
98-99	7.436992866808292E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	252.0
20-21	894.0
22-23	1943.0
24-25	4239.0
26-27	6285.0
28-29	11248.0
30-31	26843.0
32-33	32634.0
34-35	44032.0
36-37	68366.0
38-39	82170.0
40-41	81869.0
42-43	91789.0
44-45	107671.0
46-47	107565.0
48-49	102524.0
50-51	95720.0
52-53	92045.0
54-55	99086.0
56-57	102617.0
58-59	104077.0
60-61	101571.0
62-63	97410.0
64-65	100361.0
66-67	106674.0
68-69	109185.0
70-71	109063.0
72-73	103454.0
74-75	105417.0
76-77	101083.0
78-79	109630.0
80-81	112104.0
82-83	107949.0
84-85	107549.0
86-87	113918.0
88-89	122903.0
90-91	131527.0
92-93	139082.0
94-95	160025.0
96-97	244474.0
98-99	892819.0
100-101	5967533.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.07408203684513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.44292222996357	77.84791507673887
2	6.7222624918417155	11.572251463919816
3	1.615698378429497	4.172092643152144
4	0.5512987828082282	1.8981014663299318
5	0.26745811282195303	1.1510605772228284
6	0.13498968293121294	0.697146782564936
7	0.07858774653579544	0.4735057699688993
8	0.04949025280508687	0.3407862463975402
9	0.03041339254445845	0.23560243604014414
>10	0.10399925953248665	1.3838633976397463
>50	0.0023214313260014772	0.14037932905735565
>100	5.228766907434588E-4	0.07008263429161839
>500	3.5361769276870275E-5	0.01721217667614386
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.608363119259003E-6	0.0
2	0.0	2.882508935777701E-5	0.0	1.9216726238518006E-5	0.0
3	0.0	2.882508935777701E-5	0.0	2.882508935777701E-5	0.0
4	0.0	1.2490872055036703E-4	0.0	2.882508935777701E-5	9.608363119259003E-6
5	0.0	1.2490872055036703E-4	0.0	2.882508935777701E-5	9.608363119259003E-6
6	0.0	1.2490872055036703E-4	0.0	2.882508935777701E-5	1.9216726238518006E-5
7	0.0	1.2490872055036703E-4	0.0	2.882508935777701E-5	1.9216726238518006E-5
8	0.0	1.4412544678888504E-4	0.0	2.882508935777701E-5	1.9216726238518006E-5
9	0.0	2.4981744110073406E-4	0.0	7.686690495407202E-5	4.804181559629501E-5
10-11	0.0	2.594258042199931E-4	0.0	9.127944963296052E-5	4.804181559629501E-5
12-13	0.0	2.594258042199931E-4	0.0	1.0569199431184903E-4	4.804181559629501E-5
14-15	0.0	3.2188016449517657E-4	0.0	1.0569199431184903E-4	5.765017871555401E-5
16-17	0.0	3.362927091740651E-4	0.0	1.5373380990814405E-4	6.725854183481302E-5
18-19	0.0	3.459010722933241E-4	0.0	1.7775471770629155E-4	1.3451708366962604E-4
20-21	0.0	3.6511779853184214E-4	0.0	2.0657980706406855E-4	1.3932126522925553E-4
22-23	0.0	4.1796379568776663E-4	0.0	2.2099235174295707E-4	1.6814635458703255E-4
24-25	0.0	4.8041815596295016E-4	0.0	2.354048964218456E-4	2.0177562550443906E-4
26-27	0.0	5.092432453207272E-4	0.0	2.4501325954110457E-4	2.0177562550443906E-4
28-29	9.608363119259003E-6	5.140474268803567E-4	0.0	4.8522233752257965E-4	2.0177562550443906E-4
30-31	9.608363119259003E-6	5.861101502747993E-4	0.0	0.0014028210154118145	2.0657980706406855E-4
32-33	9.608363119259003E-6	5.957185133940582E-4	0.0	0.0033292978208232446	2.882508935777701E-4
34-35	9.608363119259003E-6	7.014105077059072E-4	0.0	0.006010031131096506	3.026634382566586E-4
36-37	9.608363119259003E-6	8.071025020177562E-4	0.0	0.010045543641185288	3.2188016449517657E-4
38-39	9.608363119259003E-6	8.215150466966448E-4	0.0	0.01686748145585918	3.266843460548061E-4
40-41	9.608363119259003E-6	9.416195856873824E-4	0.0	0.024145816518697873	3.314885276144356E-4
42-43	9.608363119259003E-6	0.0010088781275221954	0.0	0.030554594719243627	3.362927091740651E-4
44-45	9.608363119259003E-6	0.0011481993927514509	0.0	0.03762634997501825	3.362927091740651E-4
46-47	9.608363119259003E-6	0.0012202621161458934	0.0	0.04522656520235213	3.459010722933241E-4
48-49	9.608363119259003E-6	0.0012442830239440409	0.0	0.05319189822821784	3.843345247703601E-4
50-51	9.608363119259003E-6	0.0013499750182558899	0.0	0.060489450017295054	3.843345247703601E-4
52-53	9.608363119259003E-6	0.0013643875629347783	0.0	0.06777739344325301	4.035512510088781E-4
54-55	9.608363119259003E-6	0.0013836042891732965	0.0	0.07581959337407279	4.2757215880702567E-4
56-57	9.608363119259003E-6	0.0014028210154118145	0.0	0.08354952150351666	4.4198470348591414E-4
58-59	9.608363119259003E-6	0.0014220377416503324	0.0	0.09065490603020869	4.6600561128406164E-4
60-61	9.608363119259003E-6	0.0014604711941273683	0.0	0.09773146546754295	4.900265190822091E-4
62-63	9.608363119259003E-6	0.0014796879203658865	0.0	0.10440927783542796	4.996348822014681E-4
64-65	9.608363119259003E-6	0.0015949882777969945	0.0	0.1110582651139552	4.996348822014681E-4
66-67	9.608363119259003E-6	0.001609400822475883	0.0	0.11769764402936315	5.044390637610977E-4
68-69	9.608363119259003E-6	0.001628617548714401	0.0	0.1243802605788078	5.092432453207272E-4
70-71	9.608363119259003E-6	0.00163822591183366	0.0	0.13083227641339024	5.236557899996157E-4
72-73	9.608363119259003E-6	0.001647834274952919	0.0	0.1355884161574234	6.053268765133172E-4
74-75	9.608363119259003E-6	0.001667051001191437	0.0	0.14013317191283292	6.053268765133172E-4
76-77	9.608363119259003E-6	0.001705484453668473	0.0	0.14432241823282985	6.053268765133172E-4
78-79	9.608363119259003E-6	0.0017102886352281025	0.0	0.14796879203658864	6.053268765133172E-4
80-81	9.608363119259003E-6	0.0017102886352281025	0.0	0.14961662631154157	6.197394211922057E-4
82-83	9.608363119259003E-6	0.0017102886352281025	0.0	0.1502747991852108	6.437603289903532E-4
84-85	9.608363119259003E-6	0.001753526269264768	0.0	0.15109631423190745	6.437603289903532E-4
86-87	9.608363119259003E-6	0.0017679388139436565	0.0	0.1514758445751182	6.533686921096122E-4
88-89	9.608363119259003E-6	0.0018448057188977286	0.0	0.15161997002190708	6.869979630270188E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10735	0.0	45.7277	1
GTATCAA	18220	0.0	33.889847	1
TCAACGC	21710	0.0	27.614664	4
ATCAACG	21705	0.0	27.563282	3
CAACGCA	22120	0.0	27.12184	5
AACGCAG	23065	0.0	26.047098	6
TATCAAC	23345	0.0	26.02078	2
ACGCAGA	27025	0.0	22.230392	7
GTACATG	30135	0.0	22.070665	1
TACATGG	29390	0.0	21.834406	2
CGCAGAG	27580	0.0	21.767897	8
ACATGGG	29950	0.0	20.784384	3
CATGGGG	22120	0.0	18.018183	4
GCAGAGT	33650	0.0	17.70444	9
GAGTACT	17295	0.0	17.138847	12-13
GTGGTAT	5750	0.0	16.856356	1
AGAGTAC	28970	0.0	16.800613	10-11
CAGAGTA	31950	0.0	16.78966	10-11
TGGTATC	5630	0.0	15.212314	2
TATACTG	5845	0.0	15.153088	5
>>END_MODULE