FastQCFastQC Report
Fri 27 May 2016
SRR2139997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11767753
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137600.046.87261
GTATCAA249850.035.733811
ATCAACG298450.028.837473
TCAACGC299600.028.7954774
CAACGCA305450.028.2709885
TATCAAC315500.027.6582342
AACGCAG318600.027.1695336
ACGCAGA365650.023.6058247
CGCAGAG373000.023.1185578
TACATGG389650.021.8445512
GTACATG399100.021.812591
ACATGGG398700.020.955573
GCAGAGT446150.019.170619
GTGGTAT69250.018.9368651
CATGGGG268700.018.5857874
GAGTACT236250.017.73472612-13
TGGTATC68200.017.7170472
AGAGTAC399800.017.37546210-11
CAGAGTA430250.017.24801410-11
CTTATAC92250.015.7800751
Produced by FastQC (version 0.11.5)