##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139997_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11767753
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84168812856626	31.0	31.0	34.0	30.0	34.0
2	32.02283528554687	33.0	31.0	34.0	30.0	34.0
3	32.0842266998636	33.0	31.0	34.0	30.0	34.0
4	35.77378408605279	37.0	35.0	37.0	35.0	37.0
5	35.536928247899155	37.0	35.0	37.0	33.0	37.0
6	35.56998545091829	37.0	35.0	37.0	33.0	37.0
7	35.52878931092452	37.0	35.0	37.0	33.0	37.0
8	35.52361763541434	37.0	35.0	37.0	33.0	37.0
9	37.3020037895085	39.0	37.0	39.0	34.0	39.0
10-11	37.15391731964462	39.0	37.0	39.0	33.0	39.0
12-13	37.16085318072193	39.0	37.0	39.0	33.0	39.0
14-15	38.2727257701619	40.0	38.0	41.0	33.5	41.0
16-17	38.207972244148905	40.0	38.0	41.0	33.0	41.0
18-19	38.280469686948734	40.0	38.0	41.0	33.5	41.0
20-21	38.325597171351845	40.0	38.0	41.0	34.0	41.0
22-23	38.311604376959394	40.0	38.0	41.0	34.0	41.0
24-25	38.264858832847324	40.0	38.0	41.0	34.0	41.0
26-27	38.14932372331239	40.0	38.0	41.0	33.5	41.0
28-29	37.97967592111764	40.0	37.0	41.0	33.0	41.0
30-31	37.87593466573517	40.0	37.0	41.0	33.0	41.0
32-33	37.78666637125971	40.0	37.0	41.0	33.0	41.0
34-35	37.716617473107846	39.5	37.0	41.0	33.0	41.0
36-37	37.67467895502146	39.0	37.0	41.0	33.0	41.0
38-39	37.58634787096457	39.0	37.0	41.0	32.5	41.0
40-41	37.577720561713875	39.0	37.0	41.0	32.5	41.0
42-43	37.530052450953136	39.0	37.0	41.0	32.0	41.0
44-45	37.45748533767187	39.0	36.5	41.0	32.0	41.0
46-47	37.40728956559082	39.0	36.0	40.5	32.0	41.0
48-49	37.42146133723769	39.0	36.0	41.0	32.0	41.0
50-51	37.3158771973107	39.0	36.0	40.0	31.5	41.0
52-53	37.13630094491303	39.0	36.0	40.0	31.0	41.0
54-55	36.950842361758674	39.0	35.0	40.0	31.0	41.0
56-57	36.83374922522147	39.0	35.0	40.0	31.0	41.0
58-59	36.6456798512347	38.5	35.0	40.0	30.5	41.0
60-61	36.469371989175656	38.0	35.0	40.0	30.0	41.0
62-63	36.32907473670515	38.0	35.0	40.0	30.0	41.0
64-65	36.078749989633806	37.5	34.0	40.0	30.0	41.0
66-67	35.93968080044317	37.0	34.0	40.0	30.0	41.0
68-69	35.66955802673501	37.0	34.0	39.0	30.0	41.0
70-71	35.35753052033239	36.0	34.0	39.0	29.0	40.5
72-73	35.06421611762396	36.0	34.0	39.0	29.0	40.0
74-75	34.71530794429384	35.0	33.5	38.5	29.0	40.0
76-77	32.58075519328716	33.5	30.5	35.5	26.5	38.5
78-79	33.85307936709411	35.0	32.5	37.0	28.5	39.0
80-81	33.85414342872242	35.0	33.0	36.0	29.0	39.0
82-83	33.68621126347405	35.0	33.0	36.0	29.0	37.5
84-85	33.491980586352646	35.0	33.0	36.0	29.0	37.0
86-87	33.257558299914876	35.0	33.0	35.0	29.0	37.0
88-89	33.098676559215264	35.0	33.0	35.0	29.0	36.0
90-91	32.93913076711665	34.0	32.0	35.0	29.0	36.0
92-93	32.904753577883554	34.0	32.0	35.0	29.0	36.0
94-95	32.86337181395703	34.0	32.0	35.0	29.0	35.5
96-97	32.88624661114082	34.0	32.0	35.0	29.0	35.0
98-99	33.0225452832904	34.0	32.0	35.0	29.0	35.0
100-101	31.825486583424798	33.0	31.0	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	14.0
20	74.0
21	412.0
22	1782.0
23	6059.0
24	15947.0
25	34589.0
26	62427.0
27	100646.0
28	147932.0
29	203787.0
30	268007.0
31	345893.0
32	443875.0
33	583186.0
34	795381.0
35	1127781.0
36	1745146.0
37	2587660.0
38	2452746.0
39	835556.0
40	8848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.142229956730056	20.772087925366893	14.099582137728417	23.986099980174636
2	14.58638742298723	21.4861607090125	41.644218904116364	22.283232963883908
3	18.165038852876076	26.66943468942088	30.518181677581545	24.647344780121493
4	12.416019410339224	18.30136757675576	38.96924552010754	30.31336749279748
5	12.83369050998946	38.3026819138709	35.81879225371233	13.044835322427314
6	29.48326668651186	38.36500477193904	18.98221351178938	13.169515029759719
7	26.17436820776235	32.79717886668763	23.48506974950953	17.54338317604049
8	24.174536048176122	37.23191239036154	21.37205755947128	17.22149400199105
9	25.55395239855901	16.65485330971852	21.373995528288194	36.41719876343428
10-11	24.021693934921764	26.986297167512497	29.15530825743286	19.83670064013288
12-13	24.855704585917774	24.44748119407201	29.269813070507666	21.42700114950255
14-15	21.98483525210103	25.84258548945519	26.814529792328656	25.35804946611513
16-17	21.548682365162012	28.989971916471386	27.514252509740178	21.947093208626423
18-19	21.600551237616315	28.188201060835837	29.117163232039694	21.09408446950815
20-21	22.489961648203906	27.356223680384673	28.891510761800625	21.262303909610793
22-23	22.14740273752652	27.369613334939086	28.7309399276204	21.75204399991399
24-25	21.879673725752095	27.49841391392995	28.880414933547605	21.741497426770344
26-27	21.85525416651252	27.681708337107253	28.76468054363139	21.69835695274884
28-29	21.795093075911993	27.737569877999253	28.792667665495237	21.674669380593517
30-31	22.04017251668435	27.654887869013884	28.650438055550087	21.65450155875168
32-33	21.96695705067952	27.7065789109266	28.578030132327715	21.748433906066165
34-35	21.944470872125187	27.705009432894762	28.66662337197742	21.68389632300263
36-37	21.90597566810401	27.668924755536107	28.63343607329413	21.79166350306575
38-39	21.745073058584534	27.755598179501007	28.73969841876438	21.75963034315008
40-41	21.987427877339464	27.576268989294196	28.668013626611895	21.76828950675445
42-43	21.974401472620713	27.631356553595005	28.441344809784354	21.95289716399993
44-45	21.941294373810287	27.901501505366426	28.219028982794867	21.938175138028416
46-47	22.088863189675262	27.750744189638482	27.967921747312076	22.19247087337418
48-49	22.04325100791796	27.81752821357489	27.967109214141097	22.172111564366055
50-51	21.96543086165382	27.94694675687866	27.899590694086644	22.18803168738087
52-53	22.137914670926534	27.891443412323802	27.787075765863396	22.183566150886268
54-55	22.127537060498472	27.81591113784763	27.756573055037265	22.299978746616635
56-57	22.202432217223052	27.904157811316914	27.66167208489329	22.231737886566744
58-59	22.068650605943997	28.041846334071696	27.76685458610457	22.12264847387974
60-61	22.221583358322743	28.010299665500693	27.630549134973364	22.137567841203204
62-63	22.169265280785584	28.02615205358642	27.612640036482812	22.191942629145185
64-65	22.13470329299084	27.95436605602636	27.663483451595255	22.247447199387548
66-67	22.253365444376342	27.98306520054064	27.46562037152132	22.297948983561696
68-69	22.18825327615405	27.940661144372235	27.572148518776363	22.298937060697348
70-71	22.192020143250186	27.689933954610225	27.65626134526304	22.46178455687655
72-73	22.232440602290907	27.69503631323216	27.68550298738409	22.387020097092847
74-75	22.112031141255247	27.852884154011747	27.60273609904337	22.432348605689633
76-77	22.16120150717378	27.847637683203523	27.534645377985985	22.456515431636713
78-79	22.247142258338478	27.812389797291807	27.48750366288011	22.452964281489603
80-81	22.214703548060456	27.799183091659106	27.532893995487022	22.453219364793412
82-83	22.299983026285844	27.68267601219399	27.69202360528345	22.325317356236717
84-85	22.43654582135027	27.69031037971429	27.51652230850372	22.35662149043172
86-87	22.191796072881026	27.73849408622607	27.632213449560815	22.43749639133209
88-89	22.324400848319893	27.618014104164683	27.56708912570072	22.490495921814706
90-91	22.329154811570643	27.60411376153929	27.671744351826867	22.394987075063206
92-93	22.339441455730384	27.540306939177277	27.66458164595265	22.455669959139687
94-95	22.191054187595526	27.593019469198666	27.707661962351406	22.508264380854403
96-97	22.18462437426233	27.63003131223695	27.73883613367686	22.44650817982386
98-99	22.4160260624656	28.146021305781733	27.851049023474094	21.586903608278575
100-101	26.57269581062063	35.1403463145097	28.466710030230995	9.820247844638674
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1822.0
1	1765.0
2	2788.0
3	4180.0
4	5917.5
5	10085.0
6	13519.5
7	15765.0
8	17214.5
9	16735.0
10	15154.0
11	13639.0
12	14794.0
13	19931.5
14	31468.5
15	46726.0
16	58607.5
17	64626.5
18	65117.5
19	61806.0
20	57617.5
21	54478.0
22	54491.0
23	60652.0
24	73974.0
25	93773.5
26	119289.0
27	146635.5
28	172599.0
29	199262.5
30	223479.0
31	245712.5
32	270273.5
33	296457.5
34	322520.0
35	347379.0
36	374227.5
37	398287.5
38	414403.0
39	426536.5
40	442141.5
41	457650.0
42	467716.0
43	478867.5
44	490467.0
45	500745.0
46	509178.0
47	515494.5
48	515211.0
49	506569.0
50	497397.0
51	486411.0
52	465986.5
53	441138.0
54	413665.0
55	380384.0
56	346927.5
57	311425.0
58	275601.5
59	240324.0
60	202278.5
61	165537.5
62	131435.0
63	101825.0
64	76665.5
65	55872.0
66	40891.5
67	30448.0
68	22715.0
69	16057.0
70	11158.0
71	7771.5
72	5199.0
73	3379.0
74	2248.0
75	1579.0
76	1114.5
77	761.0
78	529.5
79	372.5
80	256.5
81	178.5
82	136.5
83	105.5
84	71.0
85	48.5
86	38.0
87	31.0
88	22.0
89	12.0
90	8.5
91	6.5
92	4.0
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.268635397088977E-4
3	2.2944057374419736E-4
4	3.3991196110251465E-5
5	0.0
6	0.0
7	0.0
8	8.327843047011608E-4
9	0.0
10-11	3.3991196110251466E-4
12-13	4.588811474883947E-4
14-15	4.248899513781433E-6
16-17	8.497799027562866E-6
18-19	4.333877504057062E-4
20-21	0.0
22-23	0.0
24-25	1.8277254819233912E-4
26-27	0.0
28-29	5.531995401805422E-4
30-31	4.7308292414856897E-4
32-33	0.0011240600261730753
34-35	0.001273945729482986
36-37	5.173859128491886E-5
38-39	0.0016985382232351863
40-41	4.380908229742013E-4
42-43	1.3705441068946592E-4
44-45	2.0556525932817162E-4
46-47	8.143473157823088E-5
48-49	4.1211916068361225E-5
50-51	3.242613049534898E-5
52-53	2.3413411258160862E-5
54-55	4.30841958931387E-4
56-57	6.037429764567073E-4
58-59	5.579764726066545E-4
60-61	5.6514564024293E-4
62-63	7.962196288243052E-5
64-65	8.06284828984972E-5
66-67	2.2470461811909776E-4
68-69	9.840390421115364E-5
70-71	1.6287239657011748E-4
72-73	0.0
74-75	1.6210395369922032E-5
76-77	7.893540261029509E-4
78-79	5.561404299076752E-6
80-81	3.389040306816597E-4
82-83	5.1644134044071384E-5
84-85	3.496506145371788E-5
86-87	3.553148678960048E-5
88-89	4.8195863168329776E-5
90-91	5.523867650095146E-5
92-93	6.887795802652378E-5
94-95	3.0709430059848837E-4
96-97	9.189587960537022E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	303.0
20-21	1005.0
22-23	2084.0
24-25	4541.0
26-27	7148.0
28-29	13808.0
30-31	31223.0
32-33	39208.0
34-35	52911.0
36-37	81143.0
38-39	97257.0
40-41	100402.0
42-43	115222.0
44-45	135075.0
46-47	134582.0
48-49	128040.0
50-51	118074.0
52-53	114567.0
54-55	124118.0
56-57	129376.0
58-59	130092.0
60-61	129233.0
62-63	124895.0
64-65	128337.0
66-67	137072.0
68-69	137101.0
70-71	135544.0
72-73	129499.0
74-75	133176.0
76-77	128278.0
78-79	137113.0
80-81	140265.0
82-83	133890.0
84-85	135344.0
86-87	141586.0
88-89	150191.0
90-91	160649.0
92-93	165503.0
94-95	185671.0
96-97	269769.0
98-99	965986.0
100-101	6438472.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.18956611925019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.19476001338398	77.73847231783462
2	6.93657504423549	11.957207868325513
3	1.6757625338341047	4.332997371301704
4	0.5930618691667247	2.044629807414061
5	0.2509703030953459	1.0815510766302288
6	0.1272126658052117	0.6578642682374624
7	0.06876176690269624	0.41485827984554846
8	0.04288663076427432	0.29571040783114333
9	0.02836985118308137	0.22006666479037323
>10	0.07965236091397983	1.05666478169211
>50	0.0016275732322424726	0.09500743820525735
>100	2.6937019769845885E-4	0.03529361901987761
>500	6.002125827404368E-5	0.032111043031002505
>1k	2.999602689441464E-5	0.03756505584110206
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.497799027562866E-6	8.497799027562866E-6
2	0.0	1.6995598055125733E-5	0.0	2.5493397082688597E-5	8.497799027562866E-6
3	0.0	1.6995598055125733E-5	0.0	4.248899513781433E-5	8.497799027562866E-6
4	0.0	5.0986794165377194E-5	0.0	5.948459319294006E-5	8.497799027562866E-6
5	0.0	5.0986794165377194E-5	0.0	7.648019124806579E-5	8.497799027562866E-6
6	1.6995598055125733E-5	5.0986794165377194E-5	0.0	7.648019124806579E-5	8.497799027562866E-6
7	1.6995598055125733E-5	5.0986794165377194E-5	0.0	1.27466985413443E-4	8.497799027562866E-6
8	1.6995598055125733E-5	5.948459319294006E-5	0.0	1.27466985413443E-4	1.6995598055125733E-5
9	1.6995598055125733E-5	9.347578930319153E-5	0.0	1.784537795788202E-4	2.5493397082688597E-5
10-11	1.6995598055125733E-5	9.347578930319153E-5	0.0	2.4643617179932314E-4	2.5493397082688597E-5
12-13	1.6995598055125733E-5	9.347578930319153E-5	0.0	2.84676267423356E-4	2.5493397082688597E-5
14-15	1.6995598055125733E-5	1.1896918638588012E-4	0.0	3.0167186547848173E-4	3.3991196110251465E-5
16-17	4.248899513781433E-5	1.1896918638588012E-4	0.0	3.3991196110251466E-4	3.3991196110251465E-5
18-19	4.248899513781433E-5	1.3171588492722443E-4	0.0	3.739031572127661E-4	3.3991196110251465E-5
20-21	4.248899513781433E-5	1.5296038249613158E-4	0.0	4.631300470021762E-4	3.3991196110251465E-5
22-23	4.248899513781433E-5	1.57209282009913E-4	0.0	5.353613387364606E-4	3.82400956240329E-5
24-25	4.248899513781433E-5	1.657070810374759E-4	0.0	6.840728217188108E-4	4.248899513781433E-5
26-27	4.248899513781433E-5	1.6995598055125733E-4	0.0	0.0010707226774729212	5.0986794165377194E-5
28-29	4.248899513781433E-5	1.6995598055125733E-4	0.0	0.0032716526256117034	5.5235693679158625E-5
30-31	4.6737894651595764E-5	1.8695157860638305E-4	0.0	0.00705742209239096	5.948459319294006E-5
32-33	5.0986794165377194E-5	1.8695157860638305E-4	0.0	0.013732443228541592	6.37334927067215E-5
34-35	5.0986794165377194E-5	2.5068507131310457E-4	0.0	0.02315225345059503	7.648019124806579E-5
36-37	5.948459319294006E-5	3.14418564019826E-4	0.0	0.03530835495952371	7.648019124806579E-5
38-39	5.948459319294006E-5	3.14418564019826E-4	0.0	0.0512374792366903	8.497799027562867E-5
40-41	5.948459319294006E-5	3.3991196110251466E-4	0.0	0.06770196485259336	9.347578930319153E-5
42-43	5.948459319294006E-5	3.441608606162961E-4	0.0	0.08024896511678993	9.347578930319153E-5
44-45	6.37334927067215E-5	3.739031572127661E-4	0.0	0.09530281609411755	9.347578930319153E-5
46-47	6.798239222050293E-5	3.993965542954547E-4	0.0	0.10918397080564149	9.347578930319153E-5
48-49	6.798239222050293E-5	4.0789435332301755E-4	0.0	0.1213103300179737	9.347578930319153E-5
50-51	7.648019124806579E-5	4.5463224797461334E-4	0.0	0.13282484770032138	9.347578930319153E-5
52-53	7.648019124806579E-5	4.588811474883948E-4	0.0	0.14326864270519613	9.347578930319153E-5
54-55	7.648019124806579E-5	4.588811474883948E-4	0.0	0.1535509795285472	9.772468881697296E-5
56-57	7.648019124806579E-5	4.758767455435205E-4	0.0	0.16413923711689055	1.0197358833075439E-4
58-59	7.648019124806579E-5	4.758767455435205E-4	0.0	0.17388621260150516	1.0197358833075439E-4
60-61	8.497799027562867E-5	4.758767455435205E-4	0.0	0.18283864387704263	1.0197358833075439E-4
62-63	1.0197358833075439E-4	4.8437454457108336E-4	0.0	0.19118773142162315	1.1896918638588012E-4
64-65	1.0197358833075439E-4	5.09867941653772E-4	0.0	0.19970677494675493	1.1896918638588012E-4
66-67	1.0197358833075439E-4	5.09867941653772E-4	0.0	0.2086124683276408	1.1896918638588012E-4
68-69	1.0197358833075439E-4	5.268635397088977E-4	0.0	0.21775610008129845	1.1896918638588012E-4
70-71	1.0197358833075439E-4	5.438591377640234E-4	0.0	0.22589699154970366	1.3171588492722443E-4
72-73	1.0197358833075439E-4	5.438591377640234E-4	0.0	0.23205364694517297	1.4446258346856872E-4
74-75	1.0197358833075439E-4	5.481080372778048E-4	0.0	0.2373052867442068	1.4446258346856872E-4
76-77	1.0197358833075439E-4	5.736014343604935E-4	0.0	0.24171139553999815	1.4871148298235015E-4
78-79	1.0197358833075439E-4	5.948459319294006E-4	0.0	0.2455014139062912	1.5296038249613158E-4
80-81	1.0197358833075439E-4	5.948459319294006E-4	0.0	0.24727320500353805	1.6145818152369447E-4
82-83	1.0197358833075439E-4	5.948459319294006E-4	0.0	0.24803800691601874	1.6995598055125733E-4
84-85	1.0197358833075439E-4	5.948459319294006E-4	0.0	0.24885804452217852	1.6995598055125733E-4
86-87	1.0197358833075439E-4	5.948459319294006E-4	0.0	0.24928293447355668	1.6995598055125733E-4
88-89	1.0197358833075439E-4	6.28837128039652E-4	0.0	0.24945289045410793	1.6995598055125733E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13760	0.0	46.8726	1
GTATCAA	24985	0.0	35.73381	1
ATCAACG	29845	0.0	28.83747	3
TCAACGC	29960	0.0	28.795477	4
CAACGCA	30545	0.0	28.270988	5
TATCAAC	31550	0.0	27.658234	2
AACGCAG	31860	0.0	27.169533	6
ACGCAGA	36565	0.0	23.605824	7
CGCAGAG	37300	0.0	23.118557	8
TACATGG	38965	0.0	21.844551	2
GTACATG	39910	0.0	21.81259	1
ACATGGG	39870	0.0	20.95557	3
GCAGAGT	44615	0.0	19.17061	9
GTGGTAT	6925	0.0	18.936865	1
CATGGGG	26870	0.0	18.585787	4
GAGTACT	23625	0.0	17.734726	12-13
TGGTATC	6820	0.0	17.717047	2
AGAGTAC	39980	0.0	17.375462	10-11
CAGAGTA	43025	0.0	17.248014	10-11
CTTATAC	9225	0.0	15.780075	1
>>END_MODULE