FastQCFastQC Report
Fri 27 May 2016
SRR2139996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11420622
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113050.044.58751
GTATCAA196200.035.9461141
ATCAACG231500.029.4943
TCAACGC233700.029.1982254
CAACGCA239100.028.5387925
TATCAAC248600.027.875262
AACGCAG251850.027.2286686
ACGCAGA288050.023.7038367
CGCAGAG292900.023.3255928
TACATGG301250.021.3442762
GTACATG308600.021.3437731
ACATGGG307950.020.301393
GCAGAGT361300.018.8627839
GAGTACT192550.017.9831112-13
AGAGTAC314500.017.50823210-11
CATGGGG228200.017.5031284
CAGAGTA346600.016.91936310-11
GTGGTAT64650.015.7245941
GTACTTT222000.015.52177214-15
AGTACTT214600.015.23730412-13
Produced by FastQC (version 0.11.5)