##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139996_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11420622
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.453710139430235	34.0	31.0	34.0	31.0	34.0
2	32.63591694042584	34.0	31.0	34.0	31.0	34.0
3	32.72602840720934	34.0	31.0	34.0	31.0	34.0
4	36.2056200616744	37.0	37.0	37.0	35.0	37.0
5	36.011809777085695	37.0	35.0	37.0	35.0	37.0
6	36.07769638116032	37.0	35.0	37.0	35.0	37.0
7	36.06374144945871	37.0	35.0	37.0	35.0	37.0
8	36.064169534724115	37.0	35.0	37.0	35.0	37.0
9	37.93913658993354	39.0	38.0	39.0	35.0	39.0
10-11	37.79050528071063	39.0	38.0	39.0	35.0	39.0
12-13	37.79488700352748	39.0	38.0	39.0	35.0	39.0
14-15	39.15830932851118	40.0	39.0	41.0	36.0	41.0
16-17	39.12228742882831	40.0	39.0	41.0	36.0	41.0
18-19	39.15537634465093	40.0	39.0	41.0	36.0	41.0
20-21	39.1608626058853	40.0	39.0	41.0	36.0	41.0
22-23	39.09809201713434	40.0	39.0	41.0	36.0	41.0
24-25	39.1206763972412	40.0	39.0	41.0	36.0	41.0
26-27	39.0128093584993	40.0	39.0	41.0	36.0	41.0
28-29	38.93638503883644	40.0	38.5	41.0	35.0	41.0
30-31	38.84013560191609	40.0	38.0	41.0	35.0	41.0
32-33	38.76645585850407	40.0	38.0	41.0	35.0	41.0
34-35	38.71464984769632	40.0	38.0	41.0	35.0	41.0
36-37	38.70078713370947	40.0	38.0	41.0	35.0	41.0
38-39	38.6202326966105	40.0	38.0	41.0	35.0	41.0
40-41	38.58677324836154	40.0	38.0	41.0	35.0	41.0
42-43	38.53421565910529	40.0	38.0	41.0	34.5	41.0
44-45	38.4374620699731	40.0	38.0	41.0	34.0	41.0
46-47	38.363439307887155	40.0	38.0	41.0	34.0	41.0
48-49	38.360266093754184	40.0	38.0	41.0	34.0	41.0
50-51	38.285576121110395	40.0	38.0	41.0	34.0	41.0
52-53	38.096734279584645	40.0	37.0	41.0	33.5	41.0
54-55	37.926791135167136	40.0	37.0	41.0	33.0	41.0
56-57	37.791896706231	39.5	37.0	41.0	33.0	41.0
58-59	37.62006386037399	39.0	36.0	41.0	33.0	41.0
60-61	37.4307187735563	39.0	36.0	41.0	33.0	41.0
62-63	37.256076472420176	39.0	35.0	40.0	32.5	41.0
64-65	37.03371155087919	38.5	35.0	40.0	32.0	41.0
66-67	36.734463146432056	38.0	35.0	40.0	32.0	41.0
68-69	36.47851463669622	37.0	35.0	40.0	32.0	41.0
70-71	36.17855213219501	37.0	35.0	39.0	31.0	41.0
72-73	35.87112586451262	36.0	35.0	39.0	31.0	41.0
74-75	35.439183676156944	36.0	34.0	39.0	31.0	40.0
76-77	33.533359282937965	34.5	32.0	36.5	28.0	39.0
78-79	34.60837155968322	35.0	34.0	37.0	30.0	39.0
80-81	34.586681009998244	35.0	34.0	37.0	30.5	39.0
82-83	34.35685767840232	35.0	34.0	36.0	30.5	38.5
84-85	34.11129120122291	35.0	34.0	36.0	30.0	37.0
86-87	33.88919926198688	35.0	34.0	35.5	30.0	37.0
88-89	33.74154052134105	35.0	34.0	35.0	30.0	36.5
90-91	33.67368615308547	35.0	34.0	35.0	30.0	36.0
92-93	33.56946117875599	35.0	34.0	35.0	30.0	36.0
94-95	33.48039802994631	35.0	33.5	35.0	30.0	36.0
96-97	33.47376846795496	35.0	33.0	35.0	30.0	35.5
98-99	33.60128012794827	35.0	34.0	35.0	30.0	35.0
100-101	32.325431918951146	34.0	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	15.0
20	50.0
21	229.0
22	933.0
23	3159.0
24	8535.0
25	18365.0
26	34039.0
27	55317.0
28	81148.0
29	112845.0
30	149109.0
31	195110.0
32	257966.0
33	352790.0
34	519093.0
35	814693.0
36	1492001.0
37	2785512.0
38	3212310.0
39	1303725.0
40	23673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.04640517828188	19.979025660773996	13.799441046205715	24.17512811473841
2	14.780697794749468	20.891469688039376	41.54557641231927	22.78225610489189
3	18.628388741338906	25.68192492661514	29.943001303257656	25.746685028788303
4	12.634162747489153	17.627967337127604	38.655411634692584	31.082458280690663
5	13.418648600632139	37.713570917028065	35.49777196879224	13.370008513547557
6	30.54112114033719	37.68133644559815	18.62239202032954	13.155150393735122
7	27.00422550540878	32.19986254432744	23.031688269658293	17.764223680605483
8	24.46517460745818	37.06692253397245	21.016237200760774	17.451665657808594
9	25.593903937597773	16.791569422950666	21.09445390783581	36.52007273161575
10-11	24.404359697455714	26.63861007762371	28.85670983044775	20.100320394472835
12-13	25.120833118626408	24.073861576440798	28.98991397698537	21.815391327947424
14-15	22.203616984333337	25.509589278829807	26.44794909978172	25.838844637055136
16-17	22.01522172529786	28.665468041499974	27.092702654579544	22.226607578622616
18-19	22.05565949035	28.001513402685074	28.5727957724194	21.370031334545526
20-21	22.793959226226217	27.23501803980934	28.37132878923451	21.599693944729932
22-23	22.501314807670273	27.120378321320647	28.263471737666716	22.11483513334236
24-25	22.272799876771803	27.199238521932635	28.37834612222953	22.149615479066032
26-27	22.258617032552156	27.381999733512696	28.218700634727888	22.14068259920726
28-29	22.178461625341846	27.509928453053668	28.24871900616643	22.062890915438054
30-31	22.479244897802374	27.331042151237778	28.149971076661668	22.039741874298183
32-33	22.27169348275038	27.510875302096615	28.084506195415237	22.132925019737765
34-35	22.328318398201912	27.507892530279666	28.13541839274029	22.028370678778135
36-37	22.358897814953636	27.377110599994342	28.129322082772358	22.13466950227967
38-39	22.192060512454738	27.524513903573567	28.18976485963801	22.093660724333684
40-41	22.435991785326607	27.38018417289836	28.06589415313671	22.117929888638326
42-43	22.302889616409445	27.468685225915333	27.937743765278118	22.2906813923971
44-45	22.39518527794892	27.574105694995694	27.765704611975746	22.265004415079645
46-47	22.430133347665237	27.525619665590114	27.575573557856664	22.468673428887985
48-49	22.427978088414932	27.621154895146905	27.595499409378167	22.35536760706
50-51	22.375091738110605	27.747791540502	27.467074216867175	22.410042504520224
52-53	22.54015196459926	27.630297665864	27.35198899705274	22.477561372483994
54-55	22.52073340189927	27.49169988358228	27.439360303389414	22.548206411129033
56-57	22.46866941533005	27.585455049759883	27.45285422051873	22.493021314391335
58-59	22.431073533932143	27.651377709105297	27.420608944026505	22.496939812936056
60-61	22.52217391926026	27.598368184931154	27.440654658803425	22.438803237005164
62-63	22.497204845893183	27.648151134964777	27.37276627313002	22.481877746012028
64-65	22.43702599535695	27.641301807377467	27.376801534434463	22.54487066283112
66-67	22.5212098595034	27.660886564225585	27.218073469584375	22.59983010668664
68-69	22.46834295079265	27.70538378703222	27.258326844678297	22.56794641749683
70-71	22.471856097466162	27.471386278843173	27.3278733202685	22.728884303422173
72-73	22.5121885147131	27.50393178741721	27.26620594944795	22.717673748421742
74-75	22.391464646343863	27.604392777834907	27.264425211626104	22.739717364195126
76-77	22.52566739637763	27.5711105403274	27.163462404084015	22.739759659210954
78-79	22.516106836022725	27.61986790497013	27.10086411648876	22.76316114251838
80-81	22.4747311585918	27.63770138545909	27.145549649240625	22.742017806708485
82-83	22.546277262703686	27.55918999452464	27.233220553337834	22.66131218943384
84-85	22.655841857776338	27.56083385619717	27.10730293908225	22.676021346944243
86-87	22.49134144015859	27.606452040199926	27.16331534058144	22.738891179060044
88-89	22.538555292475497	27.558917642572318	27.186664633401335	22.71586243155085
90-91	22.53679040701601	27.549645823277665	27.25796535298383	22.655598416722498
92-93	22.53509942596013	27.545556145767513	27.236682746153186	22.682661682119175
94-95	22.424092573284135	27.6043198508823	27.26373401369778	22.707853562135785
96-97	22.431630716200623	27.59043732866564	27.333927162862786	22.64400479227095
98-99	22.677884214075437	28.186067038610023	27.401922125026356	21.734126622288187
100-101	23.48056945466828	30.777712363042404	24.370454442248914	21.3712637400404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2138.0
1	1946.5
2	2594.0
3	3686.0
4	5097.5
5	8202.0
6	10779.5
7	12541.0
8	13776.0
9	13550.5
10	12386.5
11	11363.0
12	12099.5
13	15512.0
14	23914.5
15	35098.5
16	43633.0
17	48487.5
18	49903.0
19	48191.0
20	45400.5
21	43124.0
22	43412.0
23	49046.5
24	60218.0
25	76506.5
26	97487.5
27	120095.0
28	143878.5
29	169664.5
30	192227.5
31	213682.5
32	238352.0
33	261790.0
34	284442.0
35	307181.0
36	332419.5
37	356800.5
38	374430.0
39	388872.0
40	406128.0
41	424278.0
42	438509.0
43	451206.5
44	464654.5
45	478611.5
46	487434.5
47	491901.0
48	493870.0
49	489812.5
50	482935.0
51	473358.0
52	456009.5
53	434210.5
54	410540.5
55	382332.5
56	352368.5
57	318304.0
58	282730.5
59	248436.5
60	210149.0
61	172084.0
62	138781.0
63	109544.5
64	82934.0
65	62146.5
66	47233.5
67	36120.5
68	27687.5
69	20169.0
70	14530.0
71	10512.5
72	7342.0
73	5084.0
74	3442.0
75	2296.0
76	1596.5
77	1080.0
78	725.5
79	500.5
80	361.5
81	258.0
82	182.5
83	125.5
84	81.5
85	54.0
86	34.0
87	25.5
88	23.5
89	22.5
90	18.5
91	13.5
92	7.0
93	1.5
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004894654599372958
3	8.756090517661823E-5
4	6.216824267539894E-4
5	8.756090517661823E-6
6	0.0
7	0.0018650472802619682
8	0.0010419747716017569
9	3.502436207064729E-5
10-11	1.138291767296037E-4
12-13	1.3571940302375824E-4
14-15	2.6268271552985464E-5
16-17	3.283533944123183E-4
18-19	0.0
20-21	9.63208036167236E-5
22-23	0.0
24-25	0.0
26-27	7.098098747961104E-4
28-29	2.1045496417223457E-4
30-31	4.390637667675708E-5
32-33	0.0018838387192854512
34-35	5.607293260642798E-4
36-37	0.0013969725431920627
38-39	0.003968331907888276
40-41	1.6184042049018408E-4
42-43	9.331616617869711E-4
44-45	0.0020155392131837656
46-47	6.925851419155437E-5
48-49	2.471800372178516E-4
50-51	2.730889015022479E-4
52-53	0.0
54-55	1.8685827657353475E-4
56-57	0.0017601142256106679
58-59	0.003461387248825459
60-61	0.0013164397139391294
62-63	0.001105451123897717
64-65	0.0
66-67	1.1687329187143206E-4
68-69	3.596719196065436E-5
70-71	3.6379747394625415E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	5.0150834206190026E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	270.0
20-21	971.0
22-23	1945.0
24-25	4617.0
26-27	6425.0
28-29	12171.0
30-31	27017.0
32-33	32718.0
34-35	44370.0
36-37	68321.0
38-39	79564.0
40-41	82661.0
42-43	92916.0
44-45	109471.0
46-47	110030.0
48-49	103732.0
50-51	96019.0
52-53	88303.0
54-55	94701.0
56-57	98207.0
58-59	99091.0
60-61	102376.0
62-63	96334.0
64-65	101996.0
66-67	107737.0
68-69	110308.0
70-71	109418.0
72-73	104396.0
74-75	106197.0
76-77	104159.0
78-79	111004.0
80-81	112728.0
82-83	108436.0
84-85	107050.0
86-87	112920.0
88-89	119278.0
90-91	127189.0
92-93	132134.0
94-95	146580.0
96-97	222454.0
98-99	969587.0
100-101	6954821.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.92630880130243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.51338835890107	76.02041661618942
2	7.241185821857036	12.299343663892872
3	1.8169803910090512	4.629283133182377
4	0.684780117710558	2.326233909507163
5	0.3026676719949387	1.285222408800674
6	0.1620434483295158	0.8257051159236213
7	0.08789037723732923	0.522494372254427
8	0.054235906421363826	0.36848442694874284
9	0.03314079707635776	0.2533073009785254
>10	0.10145453256629675	1.2618003106340305
>50	0.0018464467109738701	0.10678817987554161
>100	3.208678325958043E-4	0.04797863209525989
>500	3.2635503223625776E-5	0.01628321608480127
>1k	3.2626849720036476E-5	0.03665871363254608
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7512181035323645E-5
2	0.0	8.756090517661823E-6	0.0	8.756090517661823E-6	1.7512181035323645E-5
3	0.0	8.756090517661823E-6	0.0	8.756090517661823E-6	1.7512181035323645E-5
4	0.0	6.129263362363276E-5	0.0	8.756090517661823E-6	1.7512181035323645E-5
5	0.0	6.129263362363276E-5	0.0	8.756090517661823E-6	1.7512181035323645E-5
6	1.0507308621194187E-4	6.129263362363276E-5	0.0	8.756090517661823E-6	2.6268271552985468E-5
7	1.0507308621194187E-4	6.129263362363276E-5	0.0	3.502436207064729E-5	2.6268271552985468E-5
8	1.0507308621194187E-4	6.129263362363276E-5	0.0	3.502436207064729E-5	3.502436207064729E-5
9	1.0507308621194187E-4	1.6636571983557464E-4	0.0	4.378045258830911E-5	4.378045258830911E-5
10-11	1.48853538800251E-4	1.6636571983557464E-4	0.0	4.378045258830911E-5	4.378045258830911E-5
12-13	1.5760962931791282E-4	1.6636571983557464E-4	0.0	4.378045258830911E-5	4.378045258830911E-5
14-15	1.7074376509440553E-4	2.2328030820037648E-4	0.0	5.2536543105970935E-5	5.2536543105970935E-5
16-17	2.670607607886856E-4	2.276583534592074E-4	0.0	5.2536543105970935E-5	6.129263362363276E-5
18-19	2.714388060475165E-4	2.4517053449453106E-4	0.0	5.2536543105970935E-5	7.004872414129458E-5
20-21	2.714388060475165E-4	2.7581685130634737E-4	0.0	8.318285991778732E-5	9.193895043544913E-5
22-23	2.714388060475165E-4	3.152192586358256E-4	0.0	9.631699569428005E-5	1.0945113147077279E-4
24-25	2.714388060475165E-4	3.3273143967114927E-4	0.0	1.3571940302375827E-4	1.2258526724726553E-4
26-27	2.714388060475165E-4	3.4148753018881106E-4	0.0	1.8387790087089827E-4	1.2258526724726553E-4
28-29	2.801948965651783E-4	3.4148753018881106E-4	0.0	8.36206644436704E-4	1.2258526724726553E-4
30-31	2.8457294182400927E-4	3.6775580174179653E-4	0.0	0.002867619644534247	1.2258526724726553E-4
32-33	2.9332903234167106E-4	3.852679827771202E-4	0.0	0.006715921427046618	1.3134135776492734E-4
34-35	3.1084121337699474E-4	4.596947521772457E-4	0.0	0.011741917384184505	1.4009744828258916E-4
36-37	3.1521925863582564E-4	5.253654310597094E-4	0.0	0.019215240641008872	1.4009744828258916E-4
38-39	3.1521925863582564E-4	5.384995668362021E-4	0.0	0.034188155426210586	1.4009744828258916E-4
40-41	3.1521925863582564E-4	5.866580646833421E-4	0.0	0.06684837305708918	1.532315840590819E-4
42-43	3.3273143967114927E-4	5.997922004598349E-4	0.0	0.07987305770211114	1.6636571983557464E-4
44-45	3.45865575447642E-4	6.742189698599603E-4	0.0	0.0981382625219537	1.7074376509440553E-4
46-47	3.5899971122413474E-4	7.355116034835931E-4	0.0	0.11834294139145836	1.7949985561206737E-4
48-49	3.5899971122413474E-4	7.355116034835931E-4	0.0	0.13668257298070108	1.8387790087089827E-4
50-51	3.6337775648296564E-4	8.493407802131969E-4	0.0	0.15486897298588465	1.926339913885601E-4
52-53	3.6775580174179653E-4	8.580968707308586E-4	0.0	0.17871618551073665	1.97012036647391E-4
54-55	3.6775580174179653E-4	8.580968707308586E-4	0.0	0.22825814565966723	2.0576812716505285E-4
56-57	3.6775580174179653E-4	8.756090517661822E-4	0.0	0.28005917716215456	2.2328030820037648E-4
58-59	3.6775580174179653E-4	8.799870970250132E-4	0.0	0.3343381822811402	2.276583534592074E-4
60-61	3.9840211855361296E-4	9.193895043544914E-4	0.0	0.37103933568591974	2.3203639871803832E-4
62-63	4.553167069184148E-4	9.369016853898151E-4	0.0	0.3884727119065844	2.4517053449453106E-4
64-65	4.640727974360766E-4	0.0010157065000487714	0.0	0.40475904026943543	2.4954857975336195E-4
66-67	4.7282888795373843E-4	0.001033218681084095	0.0	0.4124162414271307	2.5392662501219285E-4
68-69	4.815849784714002E-4	0.0010463528168605878	0.0	0.4191321628541773	2.5392662501219285E-4
70-71	5.034752047655547E-4	0.0010594869526370804	0.0	0.4257561453307884	2.7581685130634737E-4
72-73	5.516337026126949E-4	0.0010594869526370804	0.0	0.43097477527931494	2.8895098708284016E-4
74-75	5.516337026126949E-4	0.001072621088413573	0.0	0.4350988939131336	2.8895098708284016E-4
76-77	5.516337026126949E-4	0.0011120234957430514	0.0	0.43885525674521053	2.8895098708284016E-4
78-79	5.516337026126949E-4	0.0011382917672960369	0.0	0.44223510768502805	2.8895098708284016E-4
80-81	5.516337026126949E-4	0.0011382917672960369	0.0	0.4437061308919952	2.9332903234167106E-4
82-83	5.516337026126949E-4	0.0011514259030725297	0.0	0.4442533865493491	3.064631681181638E-4
84-85	5.560117478715257E-4	0.0011645600388490223	0.0	0.4447349715278205	3.152192586358256E-4
86-87	5.691458836480185E-4	0.0011864502651431769	0.0	0.44503267860542095	3.2397534915348743E-4
88-89	5.954141552010039E-4	0.0012302307177314861	0.0	0.44510710537482107	3.2397534915348743E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11305	0.0	44.5875	1
GTATCAA	19620	0.0	35.946114	1
ATCAACG	23150	0.0	29.494	3
TCAACGC	23370	0.0	29.198225	4
CAACGCA	23910	0.0	28.538792	5
TATCAAC	24860	0.0	27.87526	2
AACGCAG	25185	0.0	27.228668	6
ACGCAGA	28805	0.0	23.703836	7
CGCAGAG	29290	0.0	23.325592	8
TACATGG	30125	0.0	21.344276	2
GTACATG	30860	0.0	21.343773	1
ACATGGG	30795	0.0	20.30139	3
GCAGAGT	36130	0.0	18.862783	9
GAGTACT	19255	0.0	17.98311	12-13
AGAGTAC	31450	0.0	17.508232	10-11
CATGGGG	22820	0.0	17.503128	4
CAGAGTA	34660	0.0	16.919363	10-11
GTGGTAT	6465	0.0	15.724594	1
GTACTTT	22200	0.0	15.521772	14-15
AGTACTT	21460	0.0	15.237304	12-13
>>END_MODULE