FastQCFastQC Report
Fri 27 May 2016
SRR2139995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109883
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.045.6751171
GTATCAA74650.035.403031
TCAACGC86650.029.6718524
ATCAACG87800.029.379383
CAACGCA89550.028.757755
TATCAAC92800.028.2968672
AACGCAG93150.027.8276256
ACGCAGA108450.023.8238817
CGCAGAG109550.023.507598
GTACATG115700.023.061081
TACATGG117550.022.2312552
ACATGGG118200.021.1446343
GAGTACT68650.019.37138412-13
GCAGAGT134200.019.2526079
CATGGGG87100.018.4188024
AGAGTAC116350.017.54368210-11
CAGAGTA127850.017.45158810-11
GTACTTT79550.016.5318614-15
AGTACTT77850.015.86199812-13
GTACCGT4101.9645086E-1015.445366
Produced by FastQC (version 0.11.5)