##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4109883
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.446374994130004	34.0	31.0	34.0	31.0	34.0
2	32.633798821037	34.0	31.0	34.0	31.0	34.0
3	32.72310744612438	34.0	31.0	34.0	31.0	34.0
4	36.20490291329461	37.0	37.0	37.0	35.0	37.0
5	36.01222638211355	37.0	35.0	37.0	35.0	37.0
6	36.072974826777305	37.0	35.0	37.0	35.0	37.0
7	36.06017178591215	37.0	35.0	37.0	35.0	37.0
8	36.060047451472464	37.0	35.0	37.0	35.0	37.0
9	37.933673051033324	39.0	38.0	39.0	35.0	39.0
10-11	37.78683395610045	39.0	38.0	39.0	35.0	39.0
12-13	37.792416718432136	39.0	38.0	39.0	35.0	39.0
14-15	39.15121026559637	40.0	39.0	41.0	36.0	41.0
16-17	39.113373665381715	40.0	39.0	41.0	36.0	41.0
18-19	39.144222353775035	40.0	39.0	41.0	36.0	41.0
20-21	39.151230313375514	40.0	39.0	41.0	36.0	41.0
22-23	39.08725656219625	40.0	39.0	41.0	36.0	41.0
24-25	39.108834778192566	40.0	39.0	41.0	36.0	41.0
26-27	39.00321703956272	40.0	39.0	41.0	36.0	41.0
28-29	38.924682069606106	40.0	38.0	41.0	35.0	41.0
30-31	38.827374196020095	40.0	38.0	41.0	35.0	41.0
32-33	38.750560826095565	40.0	38.0	41.0	35.0	41.0
34-35	38.69967537181295	40.0	38.0	41.0	35.0	41.0
36-37	38.68309518231707	40.0	38.0	41.0	35.0	41.0
38-39	38.60370231482783	40.0	38.0	41.0	35.0	41.0
40-41	38.57040900153147	40.0	38.0	41.0	35.0	41.0
42-43	38.51558827897311	40.0	38.0	41.0	34.0	41.0
44-45	38.420856617400574	40.0	38.0	41.0	34.0	41.0
46-47	38.345002786964244	40.0	38.0	41.0	34.0	41.0
48-49	38.339377539790604	40.0	38.0	41.0	34.0	41.0
50-51	38.262233854216774	40.0	38.0	41.0	34.0	41.0
52-53	38.07592784964049	40.0	37.0	41.0	33.0	41.0
54-55	37.90261984518701	40.0	37.0	41.0	33.0	41.0
56-57	37.762001259886546	39.0	36.5	41.0	33.0	41.0
58-59	37.58425262211391	39.0	36.0	41.0	33.0	41.0
60-61	37.39106952937176	39.0	36.0	40.5	32.5	41.0
62-63	37.21011661133848	39.0	35.0	40.0	32.0	41.0
64-65	36.98451540868078	38.5	35.0	40.0	32.0	41.0
66-67	36.682208782642135	38.0	35.0	40.0	32.0	41.0
68-69	36.41967963726363	37.0	35.0	40.0	31.5	41.0
70-71	36.116503728133154	37.0	35.0	39.0	31.0	41.0
72-73	35.80583851388063	36.0	34.5	39.0	31.0	41.0
74-75	35.37777380622886	36.0	34.0	39.0	31.0	40.0
76-77	33.477464074964345	34.5	32.0	36.5	28.0	39.0
78-79	34.55494965163403	35.0	34.0	37.0	30.0	39.0
80-81	34.54264185752566	35.0	34.0	36.5	30.5	39.0
82-83	34.3159023046816	35.0	34.0	36.0	30.0	38.0
84-85	34.0731535837559	35.0	34.0	36.0	30.0	37.0
86-87	33.86223655229004	35.0	34.0	35.5	30.0	37.0
88-89	33.72031378065834	35.0	34.0	35.0	30.0	36.5
90-91	33.65057868182899	35.0	34.0	35.0	30.0	36.0
92-93	33.54205753061386	35.0	33.5	35.0	30.0	36.0
94-95	33.45065057609612	35.0	33.0	35.0	30.0	36.0
96-97	33.44980128320318	35.0	33.0	35.0	30.0	35.0
98-99	33.57694374735482	35.0	33.0	35.0	30.0	35.0
100-101	32.32698773912222	34.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	18.0
21	81.0
22	315.0
23	1117.0
24	3052.0
25	6551.0
26	12024.0
27	19624.0
28	29321.0
29	40503.0
30	54405.0
31	70891.0
32	93836.0
33	128359.0
34	188365.0
35	296574.0
36	541842.0
37	1008304.0
38	1141115.0
39	464949.0
40	8630.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.280838651611255	19.78895749587032	13.670316162284912	24.259887690233516
2	14.934416488511875	20.824766249395946	41.30680858705284	22.934008675039333
3	18.65474483209007	25.55214480610392	29.870151989765155	25.92295837204085
4	12.78593686851667	17.55879204850181	38.26899994574033	31.386271137241177
5	13.530677150663415	37.650974492461216	35.309374987073845	13.508973369801524
6	30.808297949114365	37.41208691342308	18.474735168860036	13.304879968602512
7	27.31562926572891	32.04686857479336	22.791553826351223	17.84594833312651
8	24.70532329337155	36.88011926489649	20.849068930370333	17.565488511361625
9	25.814792646013995	16.650583472023513	20.84116811196434	36.69345576999815
10-11	24.530694878037178	26.507760142359405	28.702678509187436	20.25886647041598
12-13	25.33056958409451	23.93654942387556	28.774043304469526	21.958837687560404
14-15	22.41017440650958	25.34974856936247	26.24801058716785	25.992066436960098
16-17	22.193282418527875	28.475144724701888	26.909585018706178	22.421987838064055
18-19	22.254161493161728	27.825524473567736	28.338933249438004	21.581380783832532
20-21	22.97912665222852	27.03025964455682	28.187310821712796	21.803302881501864
22-23	22.718753813752713	26.945331759831436	28.06206547554082	22.273848950875035
24-25	22.483036757778667	27.052502714885485	28.155045001440353	22.3094155258955
26-27	22.44518886607789	27.205393731647337	28.071942558160544	22.27747484411423
28-29	22.391334197804966	27.272378376870236	28.076268767260448	22.260018658064347
30-31	22.677132074219326	27.13581395655292	27.973600505529504	22.213453463698254
32-33	22.41991911788092	27.323428168800447	27.966240400447223	22.290412312871414
34-35	22.5279107298421	27.342728680720786	27.950109259783527	22.179251329653585
36-37	22.528481894759967	27.266368082081215	27.896223836606858	22.308926186551957
38-39	22.369782028665192	27.381288367390656	27.999361572576813	22.24956803136734
40-41	22.625173434785832	27.20490204818402	27.89236459333458	22.277559923695573
42-43	22.481983453746054	27.31594822040889	27.737045179309888	22.465023146535167
44-45	22.572240620150623	27.39953095031135	27.592031038048365	22.436197391489664
46-47	22.62482049173218	27.374710457581436	27.39065818091863	22.609810869767756
48-49	22.55896557593708	27.491638761247373	27.383730449165267	22.56566521365028
50-51	22.54819693040641	27.565164421160894	27.29856988479518	22.588068763637516
52-53	22.753713441924823	27.44719497556301	27.18018146387492	22.618910118637245
54-55	22.697486878404074	27.362952109062295	27.234984086113506	22.704576926420124
56-57	22.706596212703936	27.490234006263613	27.21327463128721	22.58989514974524
58-59	22.66642648587339	27.5672865038924	27.177882946200775	22.588404064033433
60-61	22.745609424273773	27.512815224792686	27.195122656370245	22.546452694563293
62-63	22.704909197748286	27.56238286805075	27.12537769077513	22.60733024342583
64-65	22.698409754949022	27.51480204330432	27.144824714530518	22.641963487216145
66-67	22.750740751048014	27.5334055010669	26.9857438435452	22.73010990433989
68-69	22.656766105414388	27.546637533179712	27.115967138965903	22.68062922244
70-71	22.718104260171906	27.300012852621098	27.15482906377006	22.827053823436934
72-73	22.747834153689606	27.39723612982975	27.053808634714876	22.801121081765764
74-75	22.598911421167138	27.49882305767389	27.090242145673532	22.812023375485442
76-77	22.718411867377853	27.4549965056985	26.97518152269238	22.851410104231267
78-79	22.711525638433166	27.473572921023447	26.93094024173491	22.88396119880848
80-81	22.685745237047268	27.491328551695123	26.987358770812016	22.835567440445597
82-83	22.7263994938473	27.462255723563906	27.055708244815253	22.755636537773544
84-85	22.817938215739744	27.43923096001107	26.980845536828248	22.761985287420945
86-87	22.631957328422892	27.455942177263022	27.11476033766299	22.7973401566511
88-89	22.72246692333078	27.43555895878107	27.050255721367627	22.791718396520523
90-91	22.73664586764756	27.427710036683617	27.059562645291685	22.776081450377138
92-93	22.755972327136114	27.443155159045755	27.03822821793545	22.76264429588268
94-95	22.669551466500984	27.473406103840958	27.067760721479555	22.789281708178503
96-97	22.630598339986303	27.507690164794663	27.09251435462951	22.76919714058952
98-99	22.890310051082864	28.071918066389774	27.1648113380264	21.872960544500962
100-101	27.228026785407934	35.13319529357723	27.659185117288278	9.97959280372656
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	894.0
1	793.5
2	1029.0
3	1459.5
4	1964.0
5	3052.5
6	3960.0
7	4587.5
8	5054.0
9	5075.5
10	4693.5
11	4298.0
12	4651.0
13	6014.0
14	9132.0
15	13220.5
16	16280.5
17	17944.0
18	18181.0
19	17287.0
20	16168.5
21	15351.5
22	15515.5
23	17283.5
24	20841.5
25	26329.5
26	33238.0
27	40810.0
28	48952.5
29	57425.5
30	65223.0
31	72830.5
32	81267.5
33	90024.0
34	98466.0
35	106316.0
36	115458.5
37	123847.5
38	130412.0
39	136359.0
40	142232.0
41	148817.0
42	154000.5
43	158776.0
44	164421.5
45	169190.5
46	172493.0
47	175814.0
48	178514.5
49	178767.5
50	177209.5
51	174197.0
52	169307.5
53	162580.0
54	155017.5
55	145814.0
56	135034.0
57	123258.5
58	110452.5
59	97229.0
60	82563.5
61	67521.5
62	53955.0
63	42205.0
64	31903.5
65	23911.0
66	18150.5
67	13823.0
68	10381.0
69	7419.5
70	5257.5
71	3724.5
72	2548.5
73	1668.0
74	1086.5
75	766.0
76	540.5
77	355.0
78	238.5
79	169.0
80	122.5
81	84.0
82	61.5
83	49.5
84	35.0
85	22.0
86	16.0
87	11.0
88	5.5
89	2.0
90	1.5
91	2.0
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003917386456013468
3	2.433159289449359E-5
4	3.8930548631189744E-4
5	0.0
6	0.0
7	0.002262838139187904
8	9.732637157797436E-4
9	7.299477868348077E-5
10-11	8.516057513072757E-5
12-13	1.7032115026145513E-4
14-15	4.866318578898718E-5
16-17	2.676475218394295E-4
18-19	0.0
20-21	1.2166280448093842E-4
22-23	0.0
24-25	0.0
26-27	6.087586242834302E-4
28-29	2.4367270321115544E-4
30-31	8.5409912674465E-5
32-33	0.00195718175613026
34-35	6.258453514048019E-4
36-37	0.0014547249528108182
38-39	0.004045857687079963
40-41	1.500379783632732E-4
42-43	8.568283867244522E-4
44-45	0.0017547094239339822
46-47	1.1565619986625003E-4
48-49	1.947565309655094E-4
50-51	2.753390964865551E-4
52-53	0.0
54-55	2.1363564200915243E-4
56-57	0.0016316845632796267
58-59	0.003501756941824372
60-61	0.0011977345470560305
62-63	0.0011682634556481992
64-65	0.0
66-67	9.939145452152316E-5
68-69	1.435807075772134E-5
70-71	1.452273359671763E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	106.0
20-21	338.0
22-23	657.0
24-25	1578.0
26-27	2358.0
28-29	4630.0
30-31	9999.0
32-33	12032.0
34-35	16617.0
36-37	25172.0
38-39	30118.0
40-41	30084.0
42-43	34173.0
44-45	40770.0
46-47	40490.0
48-49	38511.0
50-51	34681.0
52-53	33795.0
54-55	36484.0
56-57	38211.0
58-59	37695.0
60-61	37313.0
62-63	35869.0
64-65	37112.0
66-67	38921.0
68-69	39374.0
70-71	39315.0
72-73	37337.0
74-75	38157.0
76-77	36934.0
78-79	39898.0
80-81	39782.0
82-83	38623.0
84-85	38342.0
86-87	40130.0
88-89	42673.0
90-91	45014.0
92-93	47524.0
94-95	52615.0
96-97	79878.0
98-99	347202.0
100-101	2489371.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.46155848523395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.07849200633412	84.20025448348373
2	5.221300432162287	9.44653948826052
3	1.044025112168996	2.8333241623358574
4	0.33883796545965017	1.2260724171778332
5	0.12836659686874824	0.580612120509636
6	0.06843316170286327	0.37143422758278233
7	0.03662304418303141	0.23190843572894218
8	0.02149973604003607	0.1555919703362252
9	0.01400596494567572	0.11403012753678426
>10	0.04703718147387399	0.6686655457834191
>50	7.333239053510201E-4	0.043710553947797944
>100	6.454747553482121E-4	0.1278564673164669
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.433159289449359E-5	0.0	0.0	0.0
3	0.0	2.433159289449359E-5	0.0	0.0	0.0
4	0.0	2.433159289449359E-5	0.0	0.0	0.0
5	0.0	2.433159289449359E-5	0.0	0.0	0.0
6	7.299477868348077E-5	2.433159289449359E-5	0.0	0.0	0.0
7	7.299477868348077E-5	2.433159289449359E-5	0.0	0.0	0.0
8	7.299477868348077E-5	4.866318578898718E-5	0.0	0.0	0.0
9	7.299477868348077E-5	2.189843360504423E-4	0.0	0.0	0.0
10-11	7.299477868348077E-5	2.189843360504423E-4	0.0	0.0	0.0
12-13	7.299477868348077E-5	2.189843360504423E-4	0.0	1.2165796447246795E-5	0.0
14-15	7.299477868348077E-5	3.528080969701571E-4	0.0	4.866318578898718E-5	0.0
16-17	7.299477868348077E-5	3.6497389341740385E-4	0.0	4.866318578898718E-5	0.0
18-19	7.299477868348077E-5	4.0147128275914426E-4	0.0	4.866318578898718E-5	0.0
20-21	7.299477868348077E-5	4.379686721008846E-4	0.0	9.732637157797436E-5	1.2165796447246795E-5
22-23	7.299477868348077E-5	4.623002649953782E-4	0.0	1.2165796447246795E-4	2.433159289449359E-5
24-25	7.299477868348077E-5	4.7446606144262496E-4	0.0	1.3382376091971475E-4	2.433159289449359E-5
26-27	7.299477868348077E-5	4.866318578898718E-4	0.0	1.9465274315594872E-4	2.433159289449359E-5
28-29	7.299477868348077E-5	4.866318578898718E-4	0.0	5.231292472316121E-4	2.433159289449359E-5
30-31	7.299477868348077E-5	5.35295043678859E-4	0.0	0.0012895744234081602	2.433159289449359E-5
32-33	7.299477868348077E-5	5.35295043678859E-4	0.0	0.0030292833153644522	4.866318578898718E-5
34-35	7.299477868348077E-5	5.96124025915093E-4	0.0	0.005584100569286279	4.866318578898718E-5
36-37	7.299477868348077E-5	6.569530081513269E-4	0.0	0.009051352556751616	4.866318578898718E-5
38-39	7.299477868348077E-5	6.569530081513269E-4	0.0	0.01416098706459527	4.866318578898718E-5
40-41	7.299477868348077E-5	6.812846010458205E-4	0.0	0.01961126387296183	4.866318578898718E-5
42-43	7.299477868348077E-5	7.177819903875609E-4	0.0	0.02475739577014723	4.866318578898718E-5
44-45	7.299477868348077E-5	8.27274158412782E-4	0.0	0.03206903943494255	4.866318578898718E-5
46-47	7.299477868348077E-5	9.124347335435096E-4	0.0	0.03880889066671728	4.866318578898718E-5
48-49	7.299477868348077E-5	9.246005299907564E-4	0.0	0.04546358132336127	4.866318578898718E-5
50-51	7.299477868348077E-5	9.854295122269903E-4	0.0	0.052410251094739194	4.866318578898718E-5
52-53	7.299477868348077E-5	9.975953086742372E-4	0.0	0.058602641486387816	6.082898223623398E-5
54-55	7.299477868348077E-5	0.001009761105121484	0.0	0.06475853448869469	7.299477868348077E-5
56-57	7.299477868348077E-5	0.0010462584944632245	0.0	0.07185119381743957	7.299477868348077E-5
58-59	7.299477868348077E-5	0.0010462584944632245	0.0	0.07851805027053083	7.299477868348077E-5
60-61	7.299477868348077E-5	0.0010584242909104711	0.0	0.08475910384796842	7.299477868348077E-5
62-63	9.732637157797436E-5	0.001070590087357718	0.0	0.09085416786803907	7.299477868348077E-5
64-65	9.732637157797436E-5	0.0012652428305136667	0.0	0.09617062091548592	7.299477868348077E-5
66-67	1.0949216802522115E-4	0.0013139060163026538	0.0	0.10192504263503366	7.299477868348077E-5
68-69	1.2165796447246795E-4	0.0013382376091971475	0.0	0.108153930416024	7.299477868348077E-5
70-71	1.2165796447246795E-4	0.0013504034056443944	0.0	0.1136650362066268	7.299477868348077E-5
72-73	1.2165796447246795E-4	0.001362569202091641	0.0	0.11858001797131451	9.732637157797436E-5
74-75	1.2165796447246795E-4	0.0014112323878806283	0.0	0.12276505194916741	9.732637157797436E-5
76-77	1.2165796447246795E-4	0.001545056148800343	0.0	0.12625663552952723	9.732637157797436E-5
78-79	1.2165796447246795E-4	0.0015815535381420833	0.0	0.12924942145554996	9.732637157797436E-5
80-81	1.2165796447246795E-4	0.0015937193345893302	0.0	0.13075798021500856	1.0949216802522115E-4
82-83	1.2165796447246795E-4	0.001618050927483824	0.0	0.13124461207289845	1.2165796447246795E-4
84-85	1.2165796447246795E-4	0.0016788799097200576	0.0	0.13165824915210483	1.2165796447246795E-4
86-87	1.2165796447246795E-4	0.0016788799097200576	0.0	0.13186506769170803	1.2165796447246795E-4
88-89	1.2165796447246795E-4	0.0018492010599815128	0.0	0.13192589667394425	1.2165796447246795E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4335	0.0	45.675117	1
GTATCAA	7465	0.0	35.40303	1
TCAACGC	8665	0.0	29.671852	4
ATCAACG	8780	0.0	29.37938	3
CAACGCA	8955	0.0	28.75775	5
TATCAAC	9280	0.0	28.296867	2
AACGCAG	9315	0.0	27.827625	6
ACGCAGA	10845	0.0	23.823881	7
CGCAGAG	10955	0.0	23.50759	8
GTACATG	11570	0.0	23.06108	1
TACATGG	11755	0.0	22.231255	2
ACATGGG	11820	0.0	21.144634	3
GAGTACT	6865	0.0	19.371384	12-13
GCAGAGT	13420	0.0	19.252607	9
CATGGGG	8710	0.0	18.418802	4
AGAGTAC	11635	0.0	17.543682	10-11
CAGAGTA	12785	0.0	17.451588	10-11
GTACTTT	7955	0.0	16.53186	14-15
AGTACTT	7785	0.0	15.861998	12-13
GTACCGT	410	1.9645086E-10	15.44536	6
>>END_MODULE