FastQCFastQC Report
Fri 27 May 2016
SRR2139994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5500313
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77050.036.61321
GTATCAA123000.031.8832111
ATCAACG136050.028.0805743
TCAACGC136750.027.967674
CAACGCA137500.027.8457855
AACGCAG143150.026.805656
TATCAAC152500.025.3838232
ACGCAGA164950.023.1607277
CGCAGAG168000.022.739638
GTACATG184400.022.7076681
TACATGG180000.022.7005022
ACATGGG179850.022.4376743
CATGGGG124500.019.949054
GCAGAGT201050.018.9386129
AGAGTAC174050.017.45534310-11
ACGACCG1450.001368678517.4485595
GAGTACT98550.017.2647112-13
CAGAGTA188600.017.01419310-11
ATGGGGG79450.016.3468135
GTACTAG16000.015.5494941
Produced by FastQC (version 0.11.5)