##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139994_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5500313
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.441633048882856	34.0	31.0	34.0	31.0	34.0
2	32.629977057669265	34.0	31.0	34.0	31.0	34.0
3	32.72084206844229	34.0	31.0	34.0	31.0	34.0
4	36.204417094081734	37.0	37.0	37.0	35.0	37.0
5	36.007350672589	37.0	35.0	37.0	35.0	37.0
6	36.07313547429028	37.0	35.0	37.0	35.0	37.0
7	36.06019766511469	37.0	35.0	37.0	35.0	37.0
8	36.06172466912338	37.0	35.0	37.0	35.0	37.0
9	37.940905181214234	39.0	38.0	39.0	35.0	39.0
10-11	37.790509376466396	39.0	38.0	39.0	35.0	39.0
12-13	37.79685710613196	39.0	38.0	39.0	35.0	39.0
14-15	39.15097695349338	40.0	39.0	41.0	36.0	41.0
16-17	39.115609693484714	40.0	39.0	41.0	36.0	41.0
18-19	39.15421276934603	40.0	39.0	41.0	36.0	41.0
20-21	39.16206307618007	40.0	39.0	41.0	36.0	41.0
22-23	39.09768519820667	40.0	39.0	41.0	36.0	41.0
24-25	39.120928221072475	40.0	39.0	41.0	36.0	41.0
26-27	39.011141222608586	40.0	39.0	41.0	35.5	41.0
28-29	38.934794771293795	40.0	38.5	41.0	35.0	41.0
30-31	38.83491045161628	40.0	38.0	41.0	35.0	41.0
32-33	38.75956998583678	40.0	38.0	41.0	35.0	41.0
34-35	38.71155913007549	40.0	38.0	41.0	35.0	41.0
36-37	38.701148074521825	40.0	38.0	41.0	35.0	41.0
38-39	38.62076913531814	40.0	38.0	41.0	35.0	41.0
40-41	38.591529009258835	40.0	38.0	41.0	35.0	41.0
42-43	38.544482316159986	40.0	38.0	41.0	34.5	41.0
44-45	38.44905973925762	40.0	38.0	41.0	34.0	41.0
46-47	38.38036172039646	40.0	38.0	41.0	34.0	41.0
48-49	38.381062088200224	40.0	38.0	41.0	34.0	41.0
50-51	38.31061464377622	40.0	38.0	41.0	34.0	41.0
52-53	38.130490734943294	40.0	37.0	41.0	33.5	41.0
54-55	37.96633721937205	40.0	37.0	41.0	33.0	41.0
56-57	37.840482287151346	40.0	37.0	41.0	33.0	41.0
58-59	37.67660936102935	39.0	36.0	41.0	33.0	41.0
60-61	37.49729175210207	39.0	36.0	41.0	33.0	41.0
62-63	37.33110643618937	39.0	35.5	40.5	32.5	41.0
64-65	37.1151730032224	39.0	35.0	40.0	32.0	41.0
66-67	36.82742064253469	38.0	35.0	40.0	32.0	41.0
68-69	36.57884284643656	37.5	35.0	40.0	32.0	41.0
70-71	36.28519085551182	37.0	35.0	39.5	31.5	41.0
72-73	35.98069078170286	36.5	35.0	39.0	31.0	41.0
74-75	35.54691657332245	36.0	34.0	39.0	31.0	40.0
76-77	33.63317809412622	34.5	32.0	36.5	28.0	39.0
78-79	34.70599492877054	35.0	34.0	37.0	30.0	39.0
80-81	34.68389567081739	35.0	34.0	37.0	31.0	39.0
82-83	34.44402644873186	35.0	34.0	36.0	31.0	38.5
84-85	34.191056932657986	35.0	34.0	36.0	30.0	37.0
86-87	33.96414974028026	35.0	34.0	36.0	30.0	37.0
88-89	33.810271525905925	35.0	34.0	35.0	30.0	36.5
90-91	33.73304163194024	35.0	34.0	35.0	30.0	36.0
92-93	33.61620302231236	35.0	34.0	35.0	30.0	36.0
94-95	33.521843087084804	35.0	34.0	35.0	30.0	36.0
96-97	33.51304912141421	35.0	34.0	35.0	30.0	35.5
98-99	33.633635683970155	35.0	34.0	35.0	30.5	35.0
100-101	32.3578895075597	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	11.0
20	20.0
21	97.0
22	408.0
23	1503.0
24	3856.0
25	8447.0
26	15428.0
27	25086.0
28	37705.0
29	52728.0
30	70781.0
31	92950.0
32	123088.0
33	169047.0
34	248738.0
35	386840.0
36	702703.0
37	1325880.0
38	1569214.0
39	653808.0
40	11975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10002230782139	20.13963568982347	13.729327767347058	24.03101423500808
2	14.629758819740161	21.055196136345	41.854977666676305	22.460067377238538
3	18.47057711292636	26.135835980153843	30.146191760100795	25.247395146818995
4	12.384126585302486	17.94874125750801	39.050333882334584	30.61679827485491
5	13.129380091642057	38.11264922559862	35.58941100261022	13.16855968014911
6	29.901261982727167	38.01883638258405	18.981319790346475	13.098581844342313
7	26.671805869053973	32.53240561033634	23.182299568834253	17.613488951775434
8	24.394078313067915	37.10197541678507	21.175283264036572	17.328663006110443
9	25.75064832685855	16.638637953628812	21.14878210545147	36.461931614061164
10-11	24.215602304091245	26.812131986183307	28.993975422808525	19.978290286916923
12-13	25.05928535411085	24.282110199535317	29.096243671600362	21.56236077475347
14-15	22.099234215959264	25.688477041432478	26.701901647188826	25.51038709541943
16-17	21.77951760396808	28.78122514905567	27.345865511916273	22.09339173505998
18-19	21.754689233140002	28.16882421054947	28.859257645883062	21.217228910427462
20-21	22.657716060998958	27.298874556065194	28.67147469901161	21.371934683924238
22-23	22.341818396066014	27.173861652174136	28.566530415405715	21.91778953635414
24-25	22.035829148045725	27.323056304778852	28.691857485556994	21.949257061618425
26-27	22.026533403402937	27.515236135535186	28.532043014196372	21.9261874468655
28-29	21.915795172367265	27.661097497830085	28.579113072054852	21.843994257747802
30-31	22.227819878229855	27.513501546290414	28.506627037085803	21.75205153839392
32-33	21.978393675230834	27.639563778004128	28.446496217055678	21.935546329709364
34-35	22.066615651393022	27.714288911037897	28.447813832891715	21.771281604677363
36-37	22.041361251479376	27.610540296377305	28.45786963434022	21.89022881780309
38-39	21.955779449249345	27.691555512261196	28.511887781017126	21.840777257472336
40-41	22.137184692010447	27.53392855656978	28.464048618114685	21.864838133305085
42-43	22.01931922853151	27.663571079912813	28.26883678504467	22.048272906511006
44-45	22.090359185220652	27.842772642887425	28.0883048062117	21.978563365680227
46-47	22.186158137745288	27.75390534821085	27.81941131204309	22.240525202000768
48-49	22.16757538558771	27.81174811915845	27.84749079329582	22.17318570195802
50-51	22.121521597711784	27.901957059010414	27.76726422958865	22.20925711368916
52-53	22.268500510080514	27.87143194070014	27.642366813247456	22.217700735971892
54-55	22.245629731472672	27.777383406691364	27.68021459838945	22.296772263446517
56-57	22.29836846436898	27.886464995342745	27.59883613186621	22.21633040842206
58-59	22.2131747096713	27.993400314463546	27.636321214142555	22.157103761722603
60-61	22.335101930241976	27.951841574399573	27.57101080414156	22.14204569121689
62-63	22.257969888664654	27.96454618942783	27.545871219394375	22.231612702513136
64-65	22.168115585878073	27.928176422328598	27.628469921889682	22.27523806990365
66-67	22.338097485495048	27.919161181349217	27.41127169499351	22.331469638162226
68-69	22.26451694543394	27.929939207888637	27.526278033645557	22.279265813031866
70-71	22.26984508681534	27.722206641302847	27.557494411900535	22.450453859981277
72-73	22.326084469428373	27.72797388662502	27.530664479498334	22.415277164448273
74-75	22.21849201704417	27.887148064364485	27.506879545488843	22.387480373102502
76-77	22.27927069525584	27.822808176532334	27.44234471787354	22.455576410338292
78-79	22.304680202217554	27.843125231169434	27.391466056690156	22.460728509922852
80-81	22.24955096979403	27.878751649687857	27.428956841199938	22.44274053931818
82-83	22.330158832224157	27.82022740337246	27.54042323109166	22.309190533311725
84-85	22.451160446165797	27.81795443229621	27.41934008434187	22.311545037196126
86-87	22.222166085592402	27.915454867292404	27.45378321388862	22.408595833226574
88-89	22.315357276461548	27.84194068716702	27.406852654793955	22.43584938157747
90-91	22.313514347599124	27.830306606193943	27.456547698679273	22.399631347527656
92-93	22.373687689787854	27.83042469161534	27.43444177495469	22.36144584364212
94-95	22.227496836507544	27.92441205490975	27.481586917050905	22.366504191531796
96-97	22.18598898740861	27.924318995809806	27.53075020352746	22.358941813254123
98-99	22.41484796940916	28.512311079071228	27.607880530505792	21.464960421013828
100-101	23.208911899637734	31.08568014888908	24.61905381911203	21.086354132361162
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1072.0
1	1013.0
2	1441.5
3	2111.5
4	2985.0
5	4753.5
6	6125.0
7	7125.0
8	7758.0
9	7498.0
10	6735.5
11	6044.5
12	6504.0
13	8619.0
14	13615.0
15	19887.5
16	24437.0
17	26773.5
18	26883.0
19	25328.0
20	23445.5
21	22135.0
22	22359.0
23	25289.0
24	31095.5
25	39425.0
26	49904.5
27	61288.0
28	72960.0
29	85080.5
30	96402.5
31	107314.0
32	118875.0
33	130546.0
34	142134.0
35	154052.0
36	166909.5
37	178584.0
38	186793.0
39	193151.0
40	200898.0
41	208931.5
42	214979.5
43	220740.0
44	226943.5
45	233258.0
46	238286.0
47	242203.0
48	244307.5
49	240699.5
50	233843.5
51	226666.0
52	217722.5
53	206367.0
54	193039.0
55	178671.0
56	163836.0
57	147171.0
58	130146.0
59	113197.0
60	94847.0
61	77096.0
62	60997.0
63	47486.0
64	35805.0
65	26190.5
66	19434.5
67	14657.5
68	10901.5
69	7601.0
70	5204.5
71	3584.0
72	2433.0
73	1619.0
74	1056.0
75	722.0
76	506.0
77	352.5
78	248.0
79	175.0
80	117.5
81	80.5
82	58.5
83	38.0
84	30.0
85	23.5
86	15.0
87	10.5
88	6.0
89	2.0
90	1.0
91	1.0
92	1.5
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004290664913069493
3	5.454235058986643E-5
4	3.999772376590205E-4
5	0.0
6	0.0
7	0.0019635246212351917
8	0.0010181238776775067
9	1.8180783529955478E-5
10-11	1.8180783529955476E-4
12-13	1.5453666000462155E-4
14-15	4.545195882488869E-5
16-17	3.4543488706915407E-4
18-19	0.0
20-21	7.272770909352729E-5
22-23	0.0
24-25	0.0
26-27	7.278645473192021E-4
28-29	1.5478235597683873E-4
30-31	1.823670402941799E-5
32-33	0.0018557392209204522
34-35	5.502575939216346E-4
36-37	0.0013728234759079615
38-39	0.0038128508101055573
40-41	1.028427416678213E-4
42-43	8.765196777725166E-4
44-45	0.001807729508862727
46-47	4.809539818420634E-5
48-49	2.1391108513495893E-4
50-51	2.2594472154326926E-4
52-53	0.0
54-55	2.1025663925387328E-4
56-57	0.0017196337463354098
58-59	0.0032512731085769814
60-61	0.0011369496117368755
62-63	0.0010551120544671825
64-65	1.0556018202375547E-5
66-67	1.6009267444738412E-4
68-69	0.0
70-71	4.3701819426922746E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	1.1733797678820144E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	190.0
20-21	700.0
22-23	1171.0
24-25	2092.0
26-27	3249.0
28-29	6276.0
30-31	13417.0
32-33	16234.0
34-35	22309.0
36-37	34474.0
38-39	41987.0
40-41	41949.0
42-43	47502.0
44-45	56209.0
46-47	56566.0
48-49	53759.0
50-51	49346.0
52-53	46642.0
54-55	50383.0
56-57	52359.0
58-59	52791.0
60-61	52209.0
62-63	49404.0
64-65	50765.0
66-67	54003.0
68-69	54298.0
70-71	53803.0
72-73	51123.0
74-75	51352.0
76-77	49914.0
78-79	53975.0
80-81	55321.0
82-83	52860.0
84-85	51800.0
86-87	53952.0
88-89	57149.0
90-91	61090.0
92-93	63212.0
94-95	69122.0
96-97	104217.0
98-99	453401.0
100-101	3307738.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.32184078726178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.67999428625593	80.97345858727766
2	6.134687449648364	10.836537764149117
3	1.299505427123371	3.4432413430971884
4	0.42263749429771025	1.4931248592835846
5	0.19341540994484638	0.8541402521475845
6	0.0955365266475195	0.5062777137558125
7	0.05310607732669999	0.32832985545393667
8	0.031226922777351843	0.220641544145391
9	0.0213293652229592	0.16954639193840043
>10	0.06625564526547247	0.8902551334758914
>50	0.001526765887342662	0.08743936848119838
>100	7.194796504925444E-4	0.16143016335225974
>500	5.914995194372948E-5	0.03557702344194991
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8180783529955478E-5	0.0	0.0	0.0
3	0.0	1.8180783529955478E-5	0.0	1.8180783529955478E-5	0.0
4	0.0	1.8180783529955478E-5	0.0	3.6361567059910956E-5	0.0
5	0.0	1.8180783529955478E-5	0.0	5.454235058986643E-5	0.0
6	5.454235058986643E-5	1.8180783529955478E-5	0.0	5.454235058986643E-5	0.0
7	5.454235058986643E-5	1.8180783529955478E-5	0.0	7.272313411982191E-5	0.0
8	5.454235058986643E-5	1.8180783529955478E-5	0.0	7.272313411982191E-5	0.0
9	5.454235058986643E-5	9.090391764977738E-5	0.0	7.272313411982191E-5	0.0
10-11	6.363274235484417E-5	9.090391764977738E-5	0.0	1.2726548470968834E-4	0.0
12-13	7.272313411982191E-5	9.090391764977738E-5	0.0	1.4544626823964382E-4	0.0
14-15	9.090391764977738E-5	1.3635587647466607E-4	0.0	1.7271744353457703E-4	1.8180783529955478E-5
16-17	1.4544626823964382E-4	1.4544626823964382E-4	0.0	1.908982270645325E-4	1.8180783529955478E-5
18-19	1.636270517695993E-4	1.4544626823964382E-4	0.0	2.4544057765439896E-4	1.8180783529955478E-5
20-21	1.636270517695993E-4	1.4544626823964382E-4	0.0	3.545252788341318E-4	2.7271175294933215E-5
22-23	1.636270517695993E-4	1.5453666000462158E-4	0.0	4.908811553087978E-4	5.454235058986643E-5
24-25	1.636270517695993E-4	1.636270517695993E-4	0.0	6.454178153134194E-4	7.272313411982191E-5
26-27	1.636270517695993E-4	1.636270517695993E-4	0.0	7.545025164931523E-4	7.272313411982191E-5
28-29	1.636270517695993E-4	1.636270517695993E-4	0.0	0.0011726605376821283	8.181352588479965E-5
30-31	1.636270517695993E-4	1.8180783529955476E-4	0.0	0.0025907616530186552	9.090391764977738E-5
32-33	1.636270517695993E-4	1.8180783529955476E-4	0.0	0.005145161738977401	9.090391764977738E-5
34-35	1.636270517695993E-4	2.09079010594488E-4	0.0	0.00811771984612512	1.0908470117973286E-4
36-37	1.636270517695993E-4	2.363501858894212E-4	0.0	0.012981079440388212	1.0908470117973286E-4
38-39	1.636270517695993E-4	2.363501858894212E-4	0.0	0.020117036975895737	1.0908470117973286E-4
40-41	1.636270517695993E-4	3.4543488706915407E-4	0.0	0.02794386428554157	1.0908470117973286E-4
42-43	1.636270517695993E-4	3.4543488706915407E-4	0.0	0.03478892928456981	1.3635587647466607E-4
44-45	1.636270517695993E-4	3.636156705991095E-4	0.0	0.04252485267656586	1.636270517695993E-4
46-47	1.636270517695993E-4	3.8179645412906503E-4	0.0	0.05081528996622556	1.636270517695993E-4
48-49	1.636270517695993E-4	3.8179645412906503E-4	0.0	0.05929662548294979	1.8180783529955476E-4
50-51	2.2725979412444345E-4	5.090619388387534E-4	0.0	0.06773250904084913	1.8180783529955476E-4
52-53	2.363501858894212E-4	5.272427223687088E-4	0.0	0.07564114987637977	1.908982270645325E-4
54-55	2.363501858894212E-4	5.363331141336866E-4	0.0	0.08343161561896568	1.9998861882951024E-4
56-57	2.363501858894212E-4	5.454235058986643E-4	0.0	0.09063120589682805	1.9998861882951024E-4
58-59	2.363501858894212E-4	5.454235058986643E-4	0.0	0.09764898833939087	1.9998861882951024E-4
60-61	2.7271175294933214E-4	5.454235058986643E-4	0.0	0.10439405902900435	2.09079010594488E-4
62-63	3.090733200092431E-4	5.454235058986643E-4	0.0	0.1105755254291892	2.4544057765439896E-4
64-65	3.090733200092431E-4	5.636042894286197E-4	0.0	0.11702970358232341	2.545309694193767E-4
66-67	3.090733200092431E-4	5.636042894286197E-4	0.0	0.12318389880721334	2.545309694193767E-4
68-69	3.090733200092431E-4	5.908754647235531E-4	0.0	0.1297744328368222	2.545309694193767E-4
70-71	3.1816371177422083E-4	5.999658564885308E-4	0.0	0.1361467974640716	2.545309694193767E-4
72-73	3.8179645412906503E-4	5.999658564885308E-4	0.0	0.14106469940892455	3.090733200092431E-4
74-75	3.8179645412906503E-4	6.090562482535084E-4	0.0	0.14479176003256544	3.090733200092431E-4
76-77	3.8179645412906503E-4	6.27237031783464E-4	0.0	0.1487642612338607	3.090733200092431E-4
78-79	3.8179645412906503E-4	6.363274235484417E-4	0.0	0.15248223146573658	3.090733200092431E-4
80-81	3.8179645412906503E-4	6.454178153134194E-4	0.0	0.1541548635504925	3.545252788341318E-4
82-83	3.8179645412906503E-4	6.545082070783972E-4	0.0	0.1548366429328658	4.18158021188976E-4
84-85	3.8179645412906503E-4	6.545082070783972E-4	0.0	0.15546387996464928	4.18158021188976E-4
86-87	3.9088684589404276E-4	6.726889906083527E-4	0.0	0.1558274956352484	4.18158021188976E-4
88-89	3.999772376590205E-4	7.454121247281746E-4	0.0	0.15597294190348804	4.18158021188976E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7705	0.0	36.6132	1
GTATCAA	12300	0.0	31.883211	1
ATCAACG	13605	0.0	28.080574	3
TCAACGC	13675	0.0	27.96767	4
CAACGCA	13750	0.0	27.845785	5
AACGCAG	14315	0.0	26.80565	6
TATCAAC	15250	0.0	25.383823	2
ACGCAGA	16495	0.0	23.160727	7
CGCAGAG	16800	0.0	22.73963	8
GTACATG	18440	0.0	22.707668	1
TACATGG	18000	0.0	22.700502	2
ACATGGG	17985	0.0	22.437674	3
CATGGGG	12450	0.0	19.94905	4
GCAGAGT	20105	0.0	18.938612	9
AGAGTAC	17405	0.0	17.455343	10-11
ACGACCG	145	0.0013686785	17.448559	5
GAGTACT	9855	0.0	17.26471	12-13
CAGAGTA	18860	0.0	17.014193	10-11
ATGGGGG	7945	0.0	16.346813	5
GTACTAG	1600	0.0	15.549494	1
>>END_MODULE