FastQCFastQC Report
Fri 27 May 2016
SRR2139993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6649807
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81400.039.0496861
GTATCAA129950.030.8331281
ATCAACG146600.026.3317553
TCAACGC146900.026.306454
CAACGCA148600.026.033455
AACGCAG155100.025.1044046
TATCAAC163100.024.15622
ACGCAGA181200.021.396057
GTACATG172100.021.1673091
CGCAGAG185050.020.9730288
TACATGG169750.020.967732
ACATGGG173250.019.843153
CATGGGG125250.017.730824
AGAGTAC189800.017.50652710-11
GCAGAGT224050.017.3596219
GAGTACT123050.017.14668512-13
CAGAGTA208700.016.5122810-11
GTACTAG16550.016.1737371
AGTACTT137550.015.20232512-13
GTACTTT142600.014.78232214-15
Produced by FastQC (version 0.11.5)