FastQCFastQC Report
Fri 27 May 2016
SRR2139992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10659112
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112100.041.0912861
GTATCAA189850.034.5281331
ATCAACG217350.028.974543
TCAACGC219050.028.7689324
CAACGCA222000.028.4247785
AACGCAG232550.027.2259356
TATCAAC238300.026.7118532
ACGCAGA265000.023.844297
CGCAGAG269100.023.4648848
GTACATG266050.020.8970431
TACATGG260850.020.6347222
ACATGGG267650.019.5736033
GCAGAGT328850.018.9831399
CATGGGG203100.018.4009484
AGAGTAC288850.017.74952710-11
GAGTACT188450.017.64849712-13
GTGGTAT61400.016.9691961
CAGAGTA318950.016.82165110-11
GTACTAG27600.016.0477051
AGTACTT209700.015.48796812-13
Produced by FastQC (version 0.11.5)