##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139992_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10659112
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43570252381249	34.0	31.0	34.0	31.0	34.0
2	32.63396387991795	34.0	31.0	34.0	31.0	34.0
3	32.726976506110454	34.0	31.0	34.0	31.0	34.0
4	36.20839878594014	37.0	37.0	37.0	35.0	37.0
5	36.00821513086644	37.0	35.0	37.0	35.0	37.0
6	36.070931330865086	37.0	35.0	37.0	35.0	37.0
7	36.059578039896756	37.0	35.0	37.0	35.0	37.0
8	36.06124619011415	37.0	35.0	37.0	35.0	37.0
9	37.9441943193767	39.0	38.0	39.0	35.0	39.0
10-11	37.79675412923703	39.0	38.0	39.0	35.0	39.0
12-13	37.80218239568174	39.0	38.0	39.0	35.0	39.0
14-15	39.16675019457531	40.0	39.0	41.0	36.0	41.0
16-17	39.131091220356815	40.0	39.0	41.0	36.0	41.0
18-19	39.16430294568629	40.0	39.0	41.0	36.0	41.0
20-21	39.173659764173244	40.0	39.0	41.0	36.0	41.0
22-23	39.11167345846525	40.0	39.0	41.0	36.0	41.0
24-25	39.13542941889673	40.0	39.0	41.0	36.0	41.0
26-27	39.029196346190915	40.0	39.0	41.0	36.0	41.0
28-29	38.951771354983265	40.0	39.0	41.0	35.0	41.0
30-31	38.85335822294627	40.0	38.0	41.0	35.0	41.0
32-33	38.7784886618996	40.0	38.0	41.0	35.0	41.0
34-35	38.729597482302665	40.0	38.0	41.0	35.0	41.0
36-37	38.71298826569617	40.0	38.0	41.0	35.0	41.0
38-39	38.63292753255234	40.0	38.0	41.0	35.0	41.0
40-41	38.60320685284768	40.0	38.0	41.0	35.0	41.0
42-43	38.55352581716673	40.0	38.0	41.0	35.0	41.0
44-45	38.45882749556735	40.0	38.0	41.0	34.0	41.0
46-47	38.384336982189325	40.0	38.0	41.0	34.0	41.0
48-49	38.38564687614705	40.0	38.0	41.0	34.0	41.0
50-51	38.31531888229274	40.0	38.0	41.0	34.0	41.0
52-53	38.13368619314549	40.0	37.0	41.0	33.5	41.0
54-55	37.96698578309386	40.0	37.0	41.0	33.0	41.0
56-57	37.83677627321642	39.5	37.0	41.0	33.0	41.0
58-59	37.66792414352574	39.0	36.0	41.0	33.0	41.0
60-61	37.48443235931752	39.0	36.0	41.0	33.0	41.0
62-63	37.316982925945986	39.0	35.5	40.5	32.5	41.0
64-65	37.09890107500786	38.5	35.0	40.0	32.0	41.0
66-67	36.80787178805885	38.0	35.0	40.0	32.0	41.0
68-69	36.55367700553787	37.0	35.0	40.0	32.0	41.0
70-71	36.2586777258807	37.0	35.0	39.5	31.5	41.0
72-73	35.95299213351249	36.5	35.0	39.0	31.0	41.0
74-75	35.521755578836434	36.0	34.0	39.0	31.0	40.0
76-77	33.61202569023205	34.5	32.0	36.5	28.0	39.0
78-79	34.67332592234182	35.0	34.0	37.0	30.0	39.0
80-81	34.649405231166085	35.0	34.0	37.0	30.5	39.0
82-83	34.415073266694925	35.0	34.0	36.0	30.5	38.5
84-85	34.16422438601688	35.0	34.0	36.0	30.0	37.0
86-87	33.93940286536661	35.0	34.0	36.0	30.0	37.0
88-89	33.79175083430738	35.0	34.0	35.0	30.0	36.5
90-91	33.71433379375849	35.0	34.0	35.0	30.0	36.0
92-93	33.601939012017496	35.0	34.0	35.0	30.0	36.0
94-95	33.51102755208204	35.0	34.0	35.0	30.0	36.0
96-97	33.500722595946215	35.0	33.5	35.0	30.0	35.5
98-99	33.62377762830158	35.0	34.0	35.0	30.5	35.0
100-101	32.345413764545455	34.0	31.5	34.5	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	12.0
20	42.0
21	220.0
22	862.0
23	2773.0
24	7431.0
25	16363.0
26	30191.0
27	49287.0
28	73862.0
29	102293.0
30	137915.0
31	179968.0
32	236864.0
33	324373.0
34	479199.0
35	752537.0
36	1370058.0
37	2576187.0
38	3048853.0
39	1249731.0
40	20085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76940818334586	20.151284647351485	13.868378529093231	24.210928640209428
2	14.633825521439745	20.998789529117257	41.763825515810524	22.603559433632476
3	18.518562994471395	25.871278183638218	30.06367323350636	25.546485588384023
4	12.606674349634059	17.82321469515744	38.82126368176754	30.74884727344096
5	13.34367253107013	37.67586830872966	35.65316698051395	13.327292179686262
6	30.197596234582434	37.88334110446369	18.768677685097536	13.150384975856333
7	26.633951546897407	32.344249172802705	23.29380824484064	17.727991035459247
8	24.13145990053295	37.35338573283609	21.21589750339783	17.299256863233133
9	25.40733001229277	16.988146007325753	21.33246784510788	36.272056135273594
10-11	24.259037739177774	26.78640040329876	28.93426088545693	20.020300972066533
12-13	24.98401952247467	24.193403954996388	29.144971392752268	21.677605129776676
14-15	22.04713723974784	25.665840096679748	26.60957746986275	25.677445193709662
16-17	21.82581099658066	28.771046498679016	27.29027569647916	22.112866808261167
18-19	21.844418184178945	28.106717520183672	28.793801022073882	21.2550632735635
20-21	22.550574534062427	27.36185869903377	28.629318105994678	21.458248660909128
22-23	22.294414304203304	27.266646296376834	28.482084536515153	21.956854862904713
24-25	22.048537049181096	27.35897872060871	28.59965674238434	21.99282748782585
26-27	22.08217576835869	27.540867246583637	28.423689905595275	21.953267079462396
28-29	21.983452516708965	27.676951786811106	28.452632975881077	21.886962720598856
30-31	22.267109663560127	27.46265536282214	28.400695309979035	21.869539663638694
32-33	22.008836180281776	27.63133726025203	28.373155831098785	21.98667072836741
34-35	22.140813881698485	27.66912219924048	28.347861740684895	21.842202178376137
36-37	22.146550978119155	27.601523071934146	28.313190225179945	21.93873572476675
38-39	21.99965584608717	27.70326482748926	28.41476042999896	21.88231889642461
40-41	22.26437763941447	27.532883774549116	28.265810336037173	21.93692824999924
42-43	22.094712915594336	27.65312963792933	28.10883252641621	22.143324920060124
44-45	22.199426727729836	27.746443654145363	27.975140922560726	22.07898869556407
46-47	22.26545103209459	27.737870423541406	27.770444998158194	22.226233546205815
48-49	22.228996908877924	27.785997976016407	27.784498988860467	22.2005061262452
50-51	22.17945666759501	27.92976175405727	27.69166772159911	22.199113856748614
52-53	22.348606644203283	27.80768380553714	27.578255752887465	22.265453797372118
54-55	22.31407670542709	27.700867397745366	27.648169634996016	22.336886261831523
56-57	22.340981232460692	27.814441662435286	27.593970201435052	22.25060690366897
58-59	22.299849801772044	27.903165327592237	27.552706306640324	22.244278563995397
60-61	22.343360953616923	27.848936145867455	27.580920317180446	22.226782583335176
62-63	22.350482524322484	27.898573909003556	27.50459617389696	22.246347392777004
64-65	22.289346352982385	27.86467731782946	27.518807129446373	22.327169199741785
66-67	22.40419417645456	27.86655380261958	27.365957060599854	22.363294960326
68-69	22.285485080239408	27.87501705298728	27.497266663635024	22.342231203138294
70-71	22.304544533866036	27.662107262696107	27.536196309645234	22.497151893792626
72-73	22.360683465746295	27.706204287190506	27.471945179482848	22.461167067580348
74-75	22.24605180819949	27.827023439860255	27.472829226238797	22.454095525701455
76-77	22.345860567176704	27.814757783111745	27.375543512334737	22.463838137376815
78-79	22.340209995169115	27.82046592696515	27.32411125111155	22.51521282675419
80-81	22.283498710437	27.859683598334144	27.400821931305337	22.455995759923518
82-83	22.36820974494976	27.774132055361385	27.526771763868506	22.330886435820354
84-85	22.46574724389621	27.764661264920704	27.419179592302118	22.350411898880974
86-87	22.269272216199774	27.809657456209063	27.47726643958723	22.443803888003934
88-89	22.360771777857213	27.79211892455199	27.42111757800315	22.425991719587646
90-91	22.337254690064924	27.811698773440813	27.469702585208584	22.38134395128568
92-93	22.363638962345675	27.775852027525293	27.46687426998363	22.393634740145398
94-95	22.24671135471713	27.84462999800378	27.503518904267548	22.40513974301154
96-97	22.279150742969538	27.825735112853234	27.53744654102058	22.35766760315665
98-99	22.493116888045883	28.41970854502243	27.62117666470147	21.465997902230217
100-101	23.236260259367487	31.0245628139441	24.701180955850372	21.03799597083804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2419.0
1	2095.0
2	2680.0
3	3843.5
4	5182.5
5	8181.5
6	10790.5
7	12850.0
8	14244.0
9	13837.0
10	12349.0
11	11123.5
12	11995.0
13	15474.0
14	23582.0
15	34388.0
16	42878.0
17	47807.0
18	49103.0
19	47524.0
20	45175.5
21	43870.0
22	45166.0
23	51055.0
24	62249.0
25	78508.5
26	98387.0
27	119782.0
28	142988.5
29	166861.5
30	188112.0
31	208711.0
32	230214.5
33	251340.5
34	272869.0
35	293774.5
36	316321.0
37	337926.5
38	355687.0
39	371622.5
40	387404.0
41	402535.0
42	414587.5
43	427509.5
44	441647.5
45	453800.5
46	460236.5
47	463467.5
48	463596.5
49	457371.5
50	448831.5
51	437255.5
52	418781.5
53	397383.0
54	375319.0
55	349135.5
56	320170.0
57	288144.5
58	256034.0
59	223533.0
60	187814.0
61	153560.0
62	123275.5
63	96552.0
64	72468.5
65	53219.0
66	39767.5
67	30428.5
68	23044.5
69	16405.0
70	11482.0
71	8214.5
72	5736.0
73	3823.0
74	2553.5
75	1766.0
76	1265.0
77	853.5
78	557.5
79	365.5
80	236.5
81	160.5
82	117.5
83	84.0
84	61.0
85	49.0
86	40.5
87	34.0
88	24.0
89	13.0
90	7.0
91	5.0
92	4.0
93	3.5
94	2.0
95	1.0
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0042217400473885626
3	1.8763289099504725E-5
4	3.37739203791085E-4
5	0.0
6	1.8763289099504725E-5
7	0.0021390149573435385
8	9.85072677723998E-4
9	2.814493364925709E-5
10-11	1.5479713507091396E-4
12-13	1.1727055687190453E-4
14-15	2.3454111374380905E-5
16-17	3.283575592413327E-4
18-19	0.0
20-21	1.219656700413942E-4
22-23	0.0
24-25	0.0
26-27	7.22934980823915E-4
28-29	2.0669631224183807E-4
30-31	5.174974837860981E-5
32-33	0.001896221048430995
34-35	5.868180783506247E-4
36-37	0.001507263130151886
38-39	0.004198807854557126
40-41	1.2062158134314158E-4
42-43	0.0010067617425868777
44-45	0.002125476237985598
46-47	1.2402731577602653E-4
48-49	2.2559956468307996E-4
50-51	2.1267392802972493E-4
52-53	0.0
54-55	1.8573682546769692E-4
56-57	0.0019334656428366766
58-59	0.0035915677572428807
60-61	0.001197435253345368
62-63	0.0011614647920768313
64-65	0.0
66-67	9.883006614861045E-5
68-69	1.1104915185377403E-5
70-71	3.370206386945528E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	3.0118865306687454E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	225.0
20-21	863.0
22-23	1737.0
24-25	4070.0
26-27	6176.0
28-29	11664.0
30-31	26892.0
32-33	32335.0
34-35	44065.0
36-37	67485.0
38-39	80815.0
40-41	80954.0
42-43	90188.0
44-45	105878.0
46-47	106724.0
48-49	101104.0
50-51	93125.0
52-53	90010.0
54-55	95984.0
56-57	99617.0
58-59	99335.0
60-61	97775.0
62-63	93824.0
64-65	96471.0
66-67	100997.0
68-69	103536.0
70-71	101965.0
72-73	97304.0
74-75	99192.0
76-77	96425.0
78-79	102714.0
80-81	103864.0
82-83	100632.0
84-85	99252.0
86-87	103737.0
88-89	109283.0
90-91	116957.0
92-93	122353.0
94-95	134596.0
96-97	203474.0
98-99	881561.0
100-101	6453954.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.02335198723931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.71607902241152	76.27961765637471
2	7.0566175278043435	11.999545518116623
3	1.815897075773307	4.631809687382174
4	0.6673106535767769	2.2694795433557204
5	0.2925688776125052	1.2437593330879788
6	0.16815365980359984	0.8578192683254376
7	0.08195962930252723	0.48779376876526975
8	0.05931384574891662	0.4034449587861565
9	0.034355309409830684	0.26289032081242947
>10	0.1054567049883404	1.3629658410819443
>50	0.001872765911522377	0.10675561706960379
>100	3.6165648719309546E-4	0.053763501537287144
>500	3.551531539114459E-5	0.021454013888428523
>1k	1.7755854343663576E-5	0.018900971416267166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.381644549752362E-6
2	0.0	1.8763289099504725E-5	0.0	0.0	9.381644549752362E-6
3	0.0	1.8763289099504725E-5	0.0	9.381644549752362E-6	1.8763289099504725E-5
4	0.0	1.1257973459702834E-4	0.0	9.381644549752362E-6	1.8763289099504725E-5
5	0.0	1.1257973459702834E-4	0.0	9.381644549752362E-6	1.8763289099504725E-5
6	0.0	1.1257973459702834E-4	0.0	9.381644549752362E-6	1.8763289099504725E-5
7	0.0	1.1257973459702834E-4	0.0	9.381644549752362E-6	1.8763289099504725E-5
8	0.0	1.3134302369653306E-4	0.0	9.381644549752362E-6	1.8763289099504725E-5
9	0.0	1.970145355447996E-4	0.0	3.752657819900945E-5	1.8763289099504725E-5
10-11	0.0	1.970145355447996E-4	0.0	7.505315639801889E-5	1.8763289099504725E-5
12-13	0.0	1.970145355447996E-4	0.0	8.443480094777125E-5	1.8763289099504725E-5
14-15	0.0	2.6268604739306613E-4	0.0	8.443480094777125E-5	1.8763289099504725E-5
16-17	0.0	2.720676919428185E-4	0.0	1.735604241704187E-4	1.8763289099504725E-5
18-19	0.0	2.955218033171994E-4	0.0	2.2046864691918053E-4	2.3454111374380905E-5
20-21	0.0	3.236667369664565E-4	0.0	2.673768696679423E-4	2.8144933649257086E-5
22-23	0.0	3.4243002606596125E-4	0.0	3.1428509241670415E-4	3.752657819900945E-5
24-25	0.0	3.8464742653984685E-4	0.0	3.6119331516546593E-4	4.690822274876181E-5
26-27	0.0	4.034107156393516E-4	0.0	4.456281161132372E-4	4.690822274876181E-5
28-29	0.0	4.034107156393516E-4	0.0	8.631112985772174E-4	5.628986729851417E-5
30-31	0.0	5.253720947861323E-4	0.0	0.0019748361777228722	7.036233412314272E-5
32-33	0.0	5.253720947861323E-4	0.0	0.004531334317530391	7.50531563980189E-5
34-35	0.0	6.098068957339036E-4	0.0	0.007913417177716119	8.443480094777125E-5
36-37	0.0	6.942416966816748E-4	0.0	0.012683983431265193	8.443480094777125E-5
38-39	0.0	7.223866303309319E-4	0.0	0.02020337153789171	9.85072677723998E-5
40-41	0.0	7.974397867289508E-4	0.0	0.028440455452574287	1.0319809004727599E-4
42-43	0.0	8.30275542653084E-4	0.0	0.036260056184792874	1.1257973459702836E-4
44-45	0.0	9.381644549752361E-4	0.0	0.04477858943596802	1.2196137914678071E-4
46-47	0.0	0.001013217611373255	0.0	0.05441822921083858	1.2196137914678071E-4
48-49	0.0	0.001013217611373255	0.0	0.06379049211604118	1.2196137914678071E-4
50-51	0.0	0.0010976524123210265	0.0	0.07335507873451372	1.2196137914678071E-4
52-53	0.0	0.0011164157014205312	0.0	0.08178917718474109	1.2665220142165688E-4
54-55	0.0	0.0011257973459702835	0.0	0.0907674110188541	1.3603384597140925E-4
56-57	0.0	0.0011351789905200357	0.0	0.09889660602121453	1.4541549052116162E-4
58-59	0.0	0.001139869812794912	0.0	0.10630341439324402	1.501063127960378E-4
60-61	0.0	0.0011539422796195404	0.0	0.11341939178423119	1.501063127960378E-4
62-63	0.0	0.0011820872132687977	0.0	0.12064794890981538	1.501063127960378E-4
64-65	0.0	0.0013275027037899592	0.0	0.1270790662486706	1.501063127960378E-4
66-67	0.0	0.001346265992889464	0.0	0.13401679239321249	1.501063127960378E-4
68-69	0.0	0.0014072466824628543	0.0	0.14071059577946082	1.688696018955425E-4
70-71	0.0	0.0014353916161121115	0.0	0.14698222516097026	1.8294206872017106E-4
72-73	0.0	0.0014400824383869876	0.0	0.15171057401404547	2.0639618009455198E-4
74-75	0.0	0.001501063127960378	0.0	0.15557112074626855	2.0639618009455198E-4
76-77	0.0	0.001580807106633273	0.0	0.15913614567517442	2.0639618009455198E-4
78-79	0.0	0.0016136428625574063	0.0	0.16269647978180546	2.0639618009455198E-4
80-81	0.0	0.0016136428625574063	0.0	0.16443208402350964	2.1577782464430434E-4
82-83	0.0	0.0016136428625574063	0.0	0.16504658174151843	2.2985029146893287E-4
84-85	4.690822274876181E-6	0.0016136428625574063	0.0	0.16560478959222869	2.3454111374380905E-4
86-87	9.381644549752362E-6	0.0016417877962066633	0.0	0.16590500221782079	2.3923193601868524E-4
88-89	9.381644549752362E-6	0.0017074593080549298	0.0	0.16596129208511928	2.4392275829356142E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11210	0.0	41.091286	1
GTATCAA	18985	0.0	34.528133	1
ATCAACG	21735	0.0	28.97454	3
TCAACGC	21905	0.0	28.768932	4
CAACGCA	22200	0.0	28.424778	5
AACGCAG	23255	0.0	27.225935	6
TATCAAC	23830	0.0	26.711853	2
ACGCAGA	26500	0.0	23.84429	7
CGCAGAG	26910	0.0	23.464884	8
GTACATG	26605	0.0	20.897043	1
TACATGG	26085	0.0	20.634722	2
ACATGGG	26765	0.0	19.573603	3
GCAGAGT	32885	0.0	18.983139	9
CATGGGG	20310	0.0	18.400948	4
AGAGTAC	28885	0.0	17.749527	10-11
GAGTACT	18845	0.0	17.648497	12-13
GTGGTAT	6140	0.0	16.969196	1
CAGAGTA	31895	0.0	16.821651	10-11
GTACTAG	2760	0.0	16.047705	1
AGTACTT	20970	0.0	15.487968	12-13
>>END_MODULE