FastQCFastQC Report
Fri 27 May 2016
SRR2139991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9728756
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC105550.041.2418061
GGTATCA91600.040.6234441
GTATCAA157750.033.9992681
TATACAC144150.032.7353553
TTATACA146950.030.8681812
ATCAACG184750.028.1744673
TCAACGC185600.028.0006354
CAACGCA187200.027.6723335
AACGCAG193450.026.9717166
TATCAAC199050.026.4857922
TACACAT198900.023.6616615
ACACATC188650.023.602956
ACATCTC189050.023.310268
ACGCAGA227500.022.8252037
CGCAGAG231100.022.5227998
GTACATG245700.021.0505981
TACATGG242850.020.6816272
CACATCT213100.020.5046737
ATACACA252850.020.1422944
ACATGGG246150.019.829153
Produced by FastQC (version 0.11.5)