##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139991_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9728756
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43323874090377	34.0	31.0	34.0	31.0	34.0
2	32.629894819029275	34.0	31.0	34.0	31.0	34.0
3	32.72445140982054	34.0	31.0	34.0	31.0	34.0
4	36.20243030044129	37.0	37.0	37.0	35.0	37.0
5	36.0067596514909	37.0	35.0	37.0	35.0	37.0
6	36.06795832889632	37.0	35.0	37.0	35.0	37.0
7	36.05411935503368	37.0	35.0	37.0	35.0	37.0
8	36.05337897260451	37.0	35.0	37.0	35.0	37.0
9	37.936760568360434	39.0	38.0	39.0	35.0	39.0
10-11	37.786285317465044	39.0	38.0	39.0	35.0	39.0
12-13	37.793726196853946	39.0	38.0	39.0	35.0	39.0
14-15	39.15184233215429	40.0	39.0	41.0	36.0	41.0
16-17	39.10651207615855	40.0	39.0	41.0	36.0	41.0
18-19	39.13825082055712	40.0	39.0	41.0	36.0	41.0
20-21	39.14650727539778	40.0	39.0	41.0	36.0	41.0
22-23	39.078792018058664	40.0	39.0	41.0	36.0	41.0
24-25	39.102761339580425	40.0	39.0	41.0	36.0	41.0
26-27	38.99228425154759	40.0	39.0	41.0	35.5	41.0
28-29	38.90905941535318	40.0	38.0	41.0	35.0	41.0
30-31	38.816976450458625	40.0	38.0	41.0	35.0	41.0
32-33	38.74284521091969	40.0	38.0	41.0	35.0	41.0
34-35	38.69433954946223	40.0	38.0	41.0	35.0	41.0
36-37	38.67834588951183	40.0	38.0	41.0	35.0	41.0
38-39	38.58320962313875	40.0	38.0	41.0	35.0	41.0
40-41	38.575967532481904	40.0	38.0	41.0	35.0	41.0
42-43	38.53967597367118	40.0	38.0	41.0	34.5	41.0
44-45	38.446626300700984	40.0	38.0	41.0	34.0	41.0
46-47	38.379682145136776	40.0	38.0	41.0	34.0	41.0
48-49	38.37720353754945	40.0	38.0	41.0	34.0	41.0
50-51	38.27885970031987	40.0	38.0	41.0	34.0	41.0
52-53	38.10341330701804	40.0	37.0	41.0	33.5	41.0
54-55	37.921934732198196	40.0	37.0	41.0	33.0	41.0
56-57	37.78613879549212	39.0	37.0	41.0	33.0	41.0
58-59	37.62657396834302	39.0	36.0	41.0	33.0	41.0
60-61	37.43445975685303	39.0	36.0	41.0	32.5	41.0
62-63	37.27235010509588	39.0	35.0	40.0	32.0	41.0
64-65	37.070737842623636	38.5	35.0	40.0	32.0	41.0
66-67	36.78963001490645	38.0	35.0	40.0	32.0	41.0
68-69	36.54093081122086	37.0	35.0	40.0	32.0	41.0
70-71	36.24157163875524	37.0	35.0	39.5	31.5	41.0
72-73	35.93251524155569	36.0	35.0	39.0	31.0	41.0
74-75	35.49764765835211	36.0	34.0	39.0	31.0	40.0
76-77	33.58330933115586	34.5	32.0	36.5	28.0	39.0
78-79	34.658989675259406	35.0	34.0	37.0	30.0	39.0
80-81	34.63071105561221	35.0	34.0	37.0	31.0	39.0
82-83	34.397060066785414	35.0	34.0	36.0	31.0	38.5
84-85	34.14720764188414	35.0	34.0	36.0	30.0	37.0
86-87	33.926915164174154	35.0	34.0	35.5	30.0	37.0
88-89	33.78070150443365	35.0	34.0	35.0	30.0	36.5
90-91	33.705411836444185	35.0	34.0	35.0	30.0	36.0
92-93	33.5962411655812	35.0	34.0	35.0	30.0	36.0
94-95	33.50547918222051	35.0	34.0	35.0	30.0	36.0
96-97	33.4989035087438	35.0	33.5	35.0	30.0	35.0
98-99	33.619459005040724	35.0	34.0	35.0	30.5	35.0
100-101	32.36926609389276	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	14.0
20	41.0
21	201.0
22	785.0
23	2708.0
24	7140.0
25	14988.0
26	27885.0
27	45748.0
28	66898.0
29	92904.0
30	124797.0
31	162485.0
32	215826.0
33	295131.0
34	436041.0
35	681032.0
36	1248979.0
37	2350105.0
38	2777531.0
39	1157240.0
40	20272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04008775633802	20.279941238119243	13.892166686059348	24.787804319483396
2	14.491260444529969	20.826366544281434	41.900864878933604	22.781508132254995
3	18.325728042871678	25.803448211353686	30.37048732037011	25.50033642540453
4	12.524147600247105	18.13524095178086	38.14455359446422	31.196057853507813
5	13.35286854763343	37.548551942303824	35.676729892290446	13.421849617772303
6	30.143154993300275	37.70319658546272	18.654563851740143	13.49908456949686
7	26.551958039305617	32.24382340071211	22.836793584397565	18.367424975584708
8	24.192386539405618	37.1915917745746	21.2850911492061	17.33093053681369
9	25.154937684697416	16.83176153154311	20.99377617885134	37.019524604908135
10-11	24.212656789854684	26.60559096693495	28.8173815609236	20.36437068228677
12-13	24.92002431764406	23.88880120480163	28.852255613613213	22.338918863941096
14-15	22.110672571363725	25.467002345213448	26.042055548337544	26.380269535085283
16-17	22.173535921747597	28.7247191268207	26.718073547881176	22.38367140355053
18-19	22.194209619400464	27.866183507942843	28.028604068187136	21.911002804469554
20-21	22.78856534330002	27.052120298209136	27.979363528922903	22.179950829567943
22-23	22.427820579285797	27.3917983325762	27.780815569448187	22.39956551868982
24-25	22.394438680608182	27.473875083672738	27.961762973001598	22.169923262717482
26-27	22.46722113424933	27.650266051760397	27.804688655999772	22.0778241579905
28-29	21.950875317088958	27.42308870287103	28.034330757987806	22.59170522205221
30-31	22.581545688992076	27.14947328748267	28.190445234480144	22.078535789045105
32-33	22.15447751256562	27.73180228658297	27.884514781403297	22.229205419448114
34-35	22.132732553760032	27.73097250631412	28.275420780537548	21.8608741593883
36-37	22.521782222052337	27.462310314382687	27.80758649125992	22.208320972305057
38-39	22.174070433341402	27.310293164767973	28.1049886022803	22.410647799610324
40-41	22.300326385969292	27.150666491022825	28.198743058456017	22.350264064551865
42-43	22.41923832527879	27.16762418751926	28.13191899793173	22.281218489270216
44-45	22.23879226519319	27.47194304434899	27.90713969418932	22.3821249962685
46-47	22.62791327907361	27.180945347280925	27.412938740205178	22.778202633440284
48-49	22.483730873440074	27.2219035336236	27.624936050679878	22.669429542256452
50-51	22.229791278906728	27.465433452889588	27.728250022067375	22.57652524613631
52-53	22.383932352490746	27.504189273959323	27.814163700068672	22.297714673481263
54-55	22.321086213295455	27.463275872234526	27.539303283346232	22.676334631123783
56-57	22.653832603234044	27.658638745870668	27.20191392305213	22.485614727843164
58-59	22.229552564560436	27.91991185678655	27.646073343073162	22.20446223557985
60-61	22.607755317719974	28.098638845939234	27.079001137894736	22.21460469844606
62-63	22.38523391510487	28.28812003978513	27.04029944241224	22.28634660269776
64-65	22.350395133380676	28.06174760183288	27.166520973445362	22.421336291341085
66-67	22.531760579304734	27.84889920304491	27.093576415863517	22.52576380178684
68-69	22.497333098580196	27.689440594856784	27.270746131352873	22.542480175210144
70-71	22.49397520086776	27.467738366257432	27.311866023296016	22.726420409578797
72-73	22.536454308877317	27.505778570640626	27.300725183965756	22.657041936516293
74-75	22.437812005481575	27.63229459917787	27.26724280151524	22.66265059382532
76-77	22.53469221925978	27.622287868758665	27.1527700482936	22.690249863687953
78-79	22.49653868170813	27.629474004351827	27.15158384670361	22.722403467236436
80-81	22.48215587775743	27.645893046151777	27.197211068362424	22.674740007728364
82-83	22.586488744228234	27.570784559774648	27.290922833660225	22.551803862336893
84-85	22.664421858176947	27.554001629421016	27.185499890181603	22.596076622220433
86-87	22.495681417775586	27.568736837305625	27.282958730351144	22.652623014567652
88-89	22.564180060553404	27.522608432530376	27.225461449848794	22.687750057067426
90-91	22.54756661653241	27.54559662900158	27.282021921084727	22.624814833381286
92-93	22.603035082132365	27.529970586308828	27.265906741253904	22.601087590304907
94-95	22.45511199894113	27.605991814513718	27.265773967424895	22.673122219120252
96-97	22.494245461946726	27.638919651152154	27.28716146496743	22.57967342193369
98-99	22.72293982331312	28.162785176639648	27.408931446775735	21.70534355327149
100-101	26.9167585117499	35.24929808008346	27.935871010257763	9.898072397908876
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1296.0
1	1269.0
2	1883.5
3	2733.0
4	3941.5
5	6570.0
6	8611.5
7	9770.5
8	10247.0
9	9673.0
10	8700.5
11	7832.5
12	8563.5
13	11525.0
14	18112.0
15	26830.0
16	33599.0
17	37305.0
18	38153.5
19	37034.5
20	35441.5
21	34282.5
22	35211.5
23	40169.0
24	49660.0
25	63414.0
26	80875.5
27	99736.0
28	119424.0
29	139850.0
30	158195.5
31	176639.5
32	197287.0
33	218195.5
34	239843.5
35	261970.5
36	284812.5
37	305799.5
38	322911.0
39	338783.5
40	356407.0
41	373877.5
42	386815.0
43	398464.5
44	411759.0
45	426961.0
46	447691.5
47	471090.5
48	473104.5
49	461830.0
50	455520.0
51	442252.0
52	417502.5
53	386615.5
54	356563.5
55	328026.5
56	300213.5
57	269569.0
58	238197.0
59	206992.5
60	172783.5
61	140222.5
62	111903.5
63	87124.0
64	65725.5
65	48772.5
66	36306.0
67	27417.0
68	20765.5
69	14915.5
70	10594.5
71	7551.0
72	5184.5
73	3522.0
74	2371.5
75	1580.0
76	1047.5
77	711.0
78	511.5
79	373.5
80	277.0
81	195.5
82	137.0
83	93.0
84	66.0
85	48.5
86	32.0
87	24.0
88	18.0
89	12.5
90	9.0
91	6.0
92	3.5
93	2.0
94	1.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0047076933577119215
3	2.0557612915772582E-5
4	4.7282509706276935E-4
5	0.0
6	0.0
7	0.002292173840108643
8	9.456501941255387E-4
9	6.167283874731775E-5
10-11	9.764866134991977E-5
12-13	1.798791130130101E-4
14-15	4.1115225831545164E-5
16-17	3.340612098813044E-4
18-19	0.0
20-21	1.027916410865385E-4
22-23	0.0
24-25	0.0
26-27	5.760277118703214E-4
28-29	1.852775475675657E-4
30-31	3.0926532630043364E-5
32-33	0.001654159839811161
34-35	4.824668186039413E-4
36-37	0.001334947159297628
38-39	0.0038410424263365826
40-41	1.695673816793254E-4
42-43	8.338205749609776E-4
44-45	0.0017813708447568235
46-47	7.097098405909809E-5
48-49	2.5391983220977485E-4
50-51	2.008657088868904E-4
52-53	0.0
54-55	2.6774155215588346E-4
56-57	0.0017125373940035935
58-59	0.0031186726788913575
60-61	0.0011065208575927184
62-63	0.0010910757497324018
64-65	1.8224305111802164E-5
66-67	1.0477400924156068E-4
68-69	0.0
70-71	4.42836932321801E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	2.723051811302585E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	211.0
20-21	754.0
22-23	1593.0
24-25	3401.0
26-27	5404.0
28-29	10775.0
30-31	26621.0
32-33	32387.0
34-35	43641.0
36-37	68056.0
38-39	80525.0
40-41	79981.0
42-43	87906.0
44-45	102581.0
46-47	102170.0
48-49	98293.0
50-51	91636.0
52-53	90628.0
54-55	103264.0
56-57	110138.0
58-59	110385.0
60-61	112308.0
62-63	109086.0
64-65	111455.0
66-67	117108.0
68-69	100516.0
70-71	95592.0
72-73	90517.0
74-75	91840.0
76-77	90080.0
78-79	95454.0
80-81	96652.0
82-83	92114.0
84-85	91503.0
86-87	95574.0
88-89	100390.0
90-91	106400.0
92-93	110104.0
94-95	120776.0
96-97	183070.0
98-99	787811.0
100-101	5680056.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.4158124907827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.78930046139921	76.69426051889423
2	7.109348776176076	12.145016039948624
3	1.737016045620907	4.451059105387087
4	0.6460219885477488	2.2072197215486833
5	0.2971369376673809	1.2690096475941197
6	0.14963235814638912	0.7668581667591368
7	0.08635881978348978	0.5163486130286301
8	0.05216038880848037	0.3564257591929184
9	0.03528992441767216	0.2712885810186402
>10	0.09623180439349607	1.1962847531593304
>50	0.001170321683652682	0.06386767209503517
>100	2.949180577780775E-4	0.040548260054576485
>500	3.725529775084322E-5	0.021813161319049475
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.0557612915772582E-5	0.0
3	0.0	0.0	0.0	2.0557612915772582E-5	1.0278806457886291E-5
4	0.0	2.0557612915772582E-5	0.0	2.0557612915772582E-5	1.0278806457886291E-5
5	0.0	2.0557612915772582E-5	0.0	2.0557612915772582E-5	1.0278806457886291E-5
6	6.167283874731775E-5	2.0557612915772582E-5	0.0	3.0836419373658875E-5	2.0557612915772582E-5
7	6.167283874731775E-5	2.0557612915772582E-5	0.0	3.0836419373658875E-5	2.0557612915772582E-5
8	6.167283874731775E-5	2.0557612915772582E-5	0.0	4.1115225831545164E-5	3.0836419373658875E-5
9	6.167283874731775E-5	5.139403228943145E-5	0.0	1.130668710367492E-4	3.0836419373658875E-5
10-11	6.681224197626088E-5	5.139403228943145E-5	0.0	1.3362448395252177E-4	3.0836419373658875E-5
12-13	7.195164520520403E-5	5.139403228943145E-5	0.0	1.7473970978406695E-4	4.1115225831545164E-5
14-15	7.195164520520403E-5	1.027880645788629E-4	0.0	1.8501851624195325E-4	5.139403228943145E-5
16-17	1.336244839525218E-4	1.027880645788629E-4	0.0	2.5697016144715727E-4	5.139403228943145E-5
18-19	1.336244839525218E-4	1.233456774946355E-4	0.0	3.289218066523613E-4	9.250925812097662E-5
20-21	1.336244839525218E-4	1.4390329041040806E-4	0.0	3.8031583894179277E-4	1.4390329041040806E-4
22-23	1.336244839525218E-4	1.4390329041040806E-4	0.0	4.368492744601674E-4	1.4390329041040806E-4
24-25	1.336244839525218E-4	1.6446090332618066E-4	0.0	4.574068873759399E-4	1.5418209686829436E-4
26-27	1.336244839525218E-4	1.7473970978406695E-4	0.0	4.8310390352065567E-4	1.5418209686829436E-4
28-29	1.3876388718146493E-4	1.7473970978406695E-4	0.0	6.527042100757795E-4	1.5932150009723752E-4
30-31	1.4390329041040806E-4	2.055761291577258E-4	0.0	0.0018296275495037597	1.6446090332618066E-4
32-33	1.4390329041040806E-4	2.158549356156121E-4	0.0	0.004296541099396469	1.696003065551238E-4
34-35	1.4390329041040806E-4	2.46691354989271E-4	0.0	0.00754978334331748	1.7473970978406695E-4
36-37	1.4390329041040806E-4	2.929459840497593E-4	0.0	0.012051900571871676	1.7473970978406695E-4
38-39	1.490426936393512E-4	3.135035969655319E-4	0.0	0.019062046576150127	1.8501851624195325E-4
40-41	1.5418209686829436E-4	3.289218066523613E-4	0.0	0.026658084548528097	1.8501851624195325E-4
42-43	1.5418209686829436E-4	3.6489762925496333E-4	0.0	0.033657951746348655	1.9015791947089638E-4
44-45	1.5932150009723752E-4	4.111522583154516E-4	0.0	0.041577772122150046	2.055761291577258E-4
46-47	1.6446090332618066E-4	4.317098712312242E-4	0.0	0.05010918148219567	2.055761291577258E-4
48-49	1.6446090332618066E-4	4.317098712312242E-4	0.0	0.058234577987154784	2.055761291577258E-4
50-51	1.9529732269983954E-4	4.625462906048831E-4	0.0	0.06653471420189797	2.055761291577258E-4
52-53	2.158549356156121E-4	4.625462906048831E-4	0.0	0.07460871667456764	2.055761291577258E-4
54-55	2.2099433884455524E-4	4.7796450029171256E-4	0.0	0.08227670629215081	2.055761291577258E-4
56-57	2.261337420734984E-4	5.036615164364282E-4	0.0	0.09036098757127839	2.2099433884455524E-4
58-59	2.261337420734984E-4	5.242191293522009E-4	0.0	0.09811634704375359	2.4155195176032784E-4
60-61	2.3127314530244157E-4	5.344979358100872E-4	0.0	0.10579461546779464	2.46691354989271E-4
62-63	2.5697016144715727E-4	5.499161454969165E-4	0.0	0.1131131256658097	2.518307582182141E-4
64-65	2.5697016144715727E-4	6.68122419762609E-4	0.0	0.12069888483172977	2.5697016144715727E-4
66-67	2.672489679050436E-4	6.732618229915521E-4	0.0	0.12796600099745536	2.5697016144715727E-4
68-69	2.672489679050436E-4	6.989588391362678E-4	0.0	0.13490933475975758	2.7238837113398675E-4
70-71	2.929459840497593E-4	7.040982423652109E-4	0.0	0.14107661863448934	2.8266717759187297E-4
72-73	3.597582260260202E-4	7.143770488230972E-4	0.0	0.1463239493312403	2.8780658082081613E-4
74-75	3.700370324839065E-4	7.297952585099267E-4	0.0	0.15079523014042084	3.032247905076456E-4
76-77	3.700370324839065E-4	7.452134681967561E-4	0.0	0.15480910406222542	3.1864300019447504E-4
78-79	3.700370324839065E-4	7.503528714256992E-4	0.0	0.15861226245164334	3.1864300019447504E-4
80-81	3.7517643571284966E-4	7.503528714256992E-4	0.0	0.1602003380493868	3.2378240342341815E-4
82-83	3.8031583894179277E-4	7.503528714256992E-4	0.0	0.1607348359851969	3.289218066523613E-4
84-85	3.8031583894179277E-4	7.606316778835855E-4	0.0	0.16136184317912794	3.340612098813045E-4
86-87	3.8031583894179277E-4	7.811892907993581E-4	0.0	0.16169590438900924	3.392006131102476E-4
88-89	3.8031583894179277E-4	8.017469037151307E-4	0.0	0.16181925006650388	3.4434001633919074E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	10555	0.0	41.241806	1
GGTATCA	9160	0.0	40.623444	1
GTATCAA	15775	0.0	33.999268	1
TATACAC	14415	0.0	32.735355	3
TTATACA	14695	0.0	30.868181	2
ATCAACG	18475	0.0	28.174467	3
TCAACGC	18560	0.0	28.000635	4
CAACGCA	18720	0.0	27.672333	5
AACGCAG	19345	0.0	26.971716	6
TATCAAC	19905	0.0	26.485792	2
TACACAT	19890	0.0	23.661661	5
ACACATC	18865	0.0	23.60295	6
ACATCTC	18905	0.0	23.31026	8
ACGCAGA	22750	0.0	22.825203	7
CGCAGAG	23110	0.0	22.522799	8
GTACATG	24570	0.0	21.050598	1
TACATGG	24285	0.0	20.681627	2
CACATCT	21310	0.0	20.504673	7
ATACACA	25285	0.0	20.142294	4
ACATGGG	24615	0.0	19.82915	3
>>END_MODULE