FastQCFastQC Report
Fri 27 May 2016
SRR2139990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11555107
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116100.042.409891
GTATCAA198300.034.7160151
ATCAACG236550.028.15513
TCAACGC237900.028.082434
CAACGCA239600.027.7966985
TATCAAC250900.027.0751322
AACGCAG250600.026.6925686
ACGCAGA288850.023.0144127
GTACATG325900.022.6624181
CGCAGAG294100.022.5608168
TACATGG322600.022.303742
ACATGGG328900.021.3080583
CATGGGG241800.019.6251644
GCAGAGT362850.018.2857729
AGAGTAC313150.017.21107310-11
GAGTACT184450.016.67148612-13
CAGAGTA350200.016.23633410-11
GTGGTAT67300.015.33441451
GTACTAG27750.015.0848611
ATGGGGG153450.014.6384975
Produced by FastQC (version 0.11.5)