##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139990_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11555107
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44049873359027	34.0	31.0	34.0	31.0	34.0
2	32.62428327145737	34.0	31.0	34.0	31.0	34.0
3	32.71750586126117	34.0	31.0	34.0	31.0	34.0
4	36.20092708791013	37.0	37.0	37.0	35.0	37.0
5	36.006940740574706	37.0	35.0	37.0	35.0	37.0
6	36.073010055207625	37.0	35.0	37.0	35.0	37.0
7	36.0579838853937	37.0	35.0	37.0	35.0	37.0
8	36.05877029091985	37.0	35.0	37.0	35.0	37.0
9	37.93495992724256	39.0	38.0	39.0	35.0	39.0
10-11	37.78581877259985	39.0	38.0	39.0	35.0	39.0
12-13	37.79000224749109	39.0	38.0	39.0	35.0	39.0
14-15	39.15039631394153	40.0	39.0	41.0	36.0	41.0
16-17	39.11190783434546	40.0	39.0	41.0	36.0	41.0
18-19	39.143717924896755	40.0	39.0	41.0	36.0	41.0
20-21	39.152022684404216	40.0	39.0	41.0	36.0	41.0
22-23	39.086609864540485	40.0	39.0	41.0	36.0	41.0
24-25	39.109313585644614	40.0	39.0	41.0	36.0	41.0
26-27	39.00177704955536	40.0	39.0	41.0	36.0	41.0
28-29	38.9223826337188	40.0	38.0	41.0	35.0	41.0
30-31	38.82747708192413	40.0	38.0	41.0	35.0	41.0
32-33	38.756336645194864	40.0	38.0	41.0	35.0	41.0
34-35	38.70636320705154	40.0	38.0	41.0	35.0	41.0
36-37	38.69182069356086	40.0	38.0	41.0	35.0	41.0
38-39	38.61033819340834	40.0	38.0	41.0	35.0	41.0
40-41	38.582843384407184	40.0	38.0	41.0	35.0	41.0
42-43	38.53338202074967	40.0	38.0	41.0	34.5	41.0
44-45	38.435806702517624	40.0	38.0	41.0	34.0	41.0
46-47	38.361934722402324	40.0	38.0	41.0	34.0	41.0
48-49	38.35847753819282	40.0	38.0	41.0	34.0	41.0
50-51	38.28122912955082	40.0	38.0	41.0	34.0	41.0
52-53	38.09444191836569	40.0	37.0	41.0	33.5	41.0
54-55	37.920910025946135	40.0	37.0	41.0	33.0	41.0
56-57	37.78075438922938	39.0	36.5	41.0	33.0	41.0
58-59	37.60540742827361	39.0	36.0	41.0	33.0	41.0
60-61	37.41364100294247	39.0	36.0	41.0	33.0	41.0
62-63	37.23775119699833	39.0	35.0	40.0	32.5	41.0
64-65	37.012138489136554	38.5	35.0	40.0	32.0	41.0
66-67	36.71428410651744	38.0	35.0	40.0	32.0	41.0
68-69	36.45447019746384	37.0	35.0	40.0	32.0	41.0
70-71	36.157341084725914	37.0	35.0	39.0	31.0	41.0
72-73	35.8489703453037	36.0	35.0	39.0	31.0	41.0
74-75	35.41760063261545	36.0	34.0	39.0	31.0	40.0
76-77	33.51324254535152	34.5	32.0	36.5	28.0	39.0
78-79	34.59382299976639	35.0	34.0	37.0	30.0	39.0
80-81	34.57833109946162	35.0	34.0	37.0	31.0	39.0
82-83	34.353067833301964	35.0	34.0	36.0	30.5	38.0
84-85	34.109549396909685	35.0	34.0	36.0	30.0	37.0
86-87	33.895136284665426	35.0	34.0	35.5	30.0	37.0
88-89	33.75470598411831	35.0	34.0	35.0	30.0	36.5
90-91	33.68441211695226	35.0	34.0	35.0	30.0	36.0
92-93	33.57937058962834	35.0	34.0	35.0	30.0	36.0
94-95	33.49101398789039	35.0	34.0	35.0	30.0	36.0
96-97	33.48275124408194	35.0	33.0	35.0	30.0	35.0
98-99	33.60599904780439	35.0	34.0	35.0	30.5	35.0
100-101	32.35401613750601	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	19.0
20	57.0
21	219.0
22	926.0
23	3085.0
24	8283.0
25	17898.0
26	32447.0
27	52942.0
28	78549.0
29	109648.0
30	146289.0
31	193378.0
32	254496.0
33	350714.0
34	522750.0
35	821500.0
36	1503696.0
37	2800832.0
38	3235850.0
39	1395367.0
40	26157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7489513511212	20.162141293888496	13.742659414577469	24.34624794041284
2	14.801332564690256	20.942543814516192	41.483687697005536	22.772435923788017
3	18.60710754189533	25.807569398324866	30.021351599890128	25.56397145988968
4	12.739044833211452	17.655873405999976	38.478665172370526	31.12641658841805
5	13.498336882269784	37.65368640094573	35.422257088966305	13.42571962781818
6	30.482175543679517	37.54805559135021	18.64183516431306	13.327933700657207
7	26.900488382735873	32.237033867618784	23.075971763252763	17.78650598639258
8	24.417730873251593	37.03093610744916	21.090282145171845	17.4610508741274
9	25.627759441001952	16.786739157582584	21.173917705450137	36.41158369596532
10-11	24.5883741480921	26.573027149154054	28.69693043578273	20.14166826697111
12-13	25.18517162694781	23.986466225146994	28.88547567474804	21.942886473157156
14-15	22.297346327940087	25.400192287363293	26.281525956305966	26.020935428390658
16-17	22.117918801011157	28.506465127428914	27.00192642710083	22.3736896444591
18-19	22.15381043204533	27.858296768692835	28.424487977480435	21.563404821781397
20-21	22.933058327309787	27.11588298258295	28.1458011385194	21.80525755158786
22-23	22.681691141947567	27.01936160333621	28.040553567526675	22.25839368718954
24-25	22.41652067913669	27.123081307006508	28.160642829652797	22.299755184204006
26-27	22.408471293037408	27.312528956695488	28.030795742008728	22.248204008258377
28-29	22.325358571758226	27.36568089903222	28.091606295305603	22.21735423390395
30-31	22.63341997436407	27.260814129356614	27.921302411807964	22.184463484471355
32-33	22.46130362670195	27.38200622179672	27.887446102720304	22.269244048781026
34-35	22.48518288953074	27.39545708426859	27.938844756514857	22.180515269685813
36-37	22.46648520595808	27.34825570256411	27.901003942492835	22.284255148984975
38-39	22.327166255748832	27.434603951336733	27.9970832044782	22.241146588436234
40-41	22.58556538897442	27.232110820222623	27.89798726203103	22.284336528771924
42-43	22.47185581469606	27.33252987507556	27.750197195031927	22.44541711519645
44-45	22.503918081019506	27.48947189764035	27.59905308705343	22.40755693428671
46-47	22.603263601531822	27.37780785520914	27.400786409005594	22.618142134253436
48-49	22.57587916514669	27.440401172969953	27.406049495875344	22.57767016600801
50-51	22.45740468587478	27.573331201655897	27.331015291789907	22.638248820679415
52-53	22.69084729591552	27.49619778198411	27.204862134546143	22.608092787554227
54-55	22.673522081975023	27.35198009390136	27.249507450868805	22.724990373254812
56-57	22.685955061920364	27.467497059565666	27.223031669077898	22.623516209436076
58-59	22.590011577046937	27.599408742790693	27.244506964115512	22.566072716046857
60-61	22.729802327073376	27.565927285041997	27.134971077104055	22.56929931078058
62-63	22.678635190565725	27.61737176874737	27.096522968500146	22.607470072186757
64-65	22.645749176553544	27.563062015857632	27.12415036497862	22.6670384426102
66-67	22.752140205143846	27.543471079683208	26.95491693640356	22.749471778769387
68-69	22.63927530206045	27.543441475969804	27.107271720238437	22.710011501731305
70-71	22.67120874395514	27.282849842214894	27.16607853062482	22.879862883205142
72-73	22.755578877196776	27.322592142153535	27.10023446450127	22.82159451614842
74-75	22.586025882252937	27.505938574473344	27.069563567084614	22.838471976189105
76-77	22.715968269946877	27.45184787516789	26.993332608235992	22.838851246649234
78-79	22.67918141524979	27.475817404564683	26.96278974787516	22.882211432310363
80-81	22.673377963594234	27.46030093245468	27.01734988185189	22.8489712220992
82-83	22.732242042237136	27.377514841973582	27.134341458694735	22.755901657094544
84-85	22.838419920867974	27.420630257857066	26.991523219954118	22.74942660132084
86-87	22.64060423858828	27.428702042988	27.10664350974158	22.82405020868214
88-89	22.731001090712056	27.38289311387998	27.035379083214828	22.850726712193136
90-91	22.73638815659144	27.40743336939793	27.1000655258065	22.756112948204134
92-93	22.798127554031023	27.358252439936827	27.091836443874328	22.751783562157826
94-95	22.616444505437666	27.44845675204744	27.115991857698972	22.819106884815916
96-97	22.663235193668676	27.455559427475528	27.13311596554686	22.748089413308932
98-99	22.89610076172753	28.05305102863343	27.173283981751993	21.877564227887046
100-101	27.208059833667093	35.0662162899799	27.688757714072654	10.036966162280354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1947.0
1	1810.0
2	2512.5
3	3553.0
4	4878.5
5	7952.0
6	10530.5
7	12207.5
8	13036.0
9	12480.0
10	11365.5
11	10360.5
12	11225.0
13	14792.5
14	22779.5
15	33399.0
16	41891.5
17	47079.0
18	48722.5
19	47181.5
20	44584.5
21	42628.0
22	43407.5
23	49245.5
24	60414.5
25	76394.5
26	97565.5
27	120570.5
28	143509.5
29	168500.0
30	191443.0
31	213455.5
32	238050.0
33	262882.0
34	287807.0
35	314219.5
36	342465.0
37	366815.0
38	385681.5
39	402542.0
40	420583.5
41	438812.0
42	453151.5
43	467851.0
44	483402.0
45	498267.0
46	511959.5
47	522711.5
48	526034.0
49	520409.5
50	512882.5
51	502799.0
52	483146.5
53	459836.5
54	435772.0
55	405617.5
56	375195.0
57	341578.0
58	305422.0
59	269179.0
60	226531.5
61	184731.0
62	148752.5
63	116567.0
64	87839.5
65	65153.5
66	49313.5
67	37965.0
68	28681.5
69	20468.0
70	14591.5
71	10278.0
72	6935.0
73	4583.5
74	3066.0
75	2078.0
76	1437.5
77	1007.0
78	713.0
79	501.0
80	343.5
81	230.0
82	158.5
83	108.5
84	73.5
85	44.0
86	25.0
87	17.5
88	10.5
89	6.5
90	7.5
91	9.0
92	9.0
93	7.0
94	4.0
95	3.0
96	2.5
97	1.5
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0047251834188986735
3	8.654182085895007E-6
4	4.932883788960154E-4
5	1.7308364171790015E-5
6	0.0
7	0.002033732790185327
8	8.481098444177107E-4
9	3.461672834358003E-5
10-11	1.211585492025301E-4
12-13	1.3846691337432012E-4
14-15	4.759800147242254E-5
16-17	3.202047371781153E-4
18-19	0.0
20-21	1.038541306422651E-4
22-23	0.0
24-25	0.0
26-27	6.279008477873944E-4
28-29	1.9500805383262327E-4
30-31	4.3408036103679056E-5
32-33	0.0018506282599905404
34-35	4.7211346005528887E-4
36-37	0.0013276578743476452
38-39	0.003878802794883544
40-41	1.2527139152177538E-4
42-43	9.12293914724094E-4
44-45	0.0018670222743061079
46-47	6.0068574284403074E-5
48-49	1.823731232519653E-4
50-51	1.986618516079217E-4
52-53	0.0
54-55	2.1747118035672717E-4
56-57	0.0016671594809648522
58-59	0.0032359463567177625
60-61	0.0011268338657748553
62-63	0.0010033861749601434
64-65	5.1274722940880195E-6
66-67	9.86382317217646E-5
68-69	5.258414804583109E-6
70-71	6.392585964033393E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	3.455638755818E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	276.0
20-21	958.0
22-23	1932.0
24-25	4215.0
26-27	6837.0
28-29	14146.0
30-31	34515.0
32-33	41848.0
34-35	56636.0
36-37	88111.0
38-39	104739.0
40-41	102960.0
42-43	112682.0
44-45	130775.0
46-47	130604.0
48-49	124062.0
50-51	114667.0
52-53	109892.0
54-55	117976.0
56-57	121806.0
58-59	121301.0
60-61	118889.0
62-63	115365.0
64-65	117613.0
66-67	123226.0
68-69	122564.0
70-71	120167.0
72-73	115430.0
74-75	115170.0
76-77	113520.0
78-79	120009.0
80-81	121383.0
82-83	116397.0
84-85	114825.0
86-87	118797.0
88-89	125276.0
90-91	132135.0
92-93	136624.0
94-95	148454.0
96-97	220130.0
98-99	930575.0
100-101	6667620.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.85947421043599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.7385578678229	76.15166837066236
2	6.993605722275271	11.86947408854752
3	1.791739664986342	4.561382576781711
4	0.6877423890905786	2.334458301218224
5	0.3147645844781785	1.3355378569442296
6	0.16241849858404792	0.8269649035134448
7	0.09724773397440883	0.577667410125728
8	0.06122928546817555	0.4156707976888052
9	0.03690581458270423	0.281862721871651
>10	0.11360359241421134	1.4445150412267456
>50	0.0017735334148728747	0.10059409910296163
>100	3.6151142421462617E-4	0.050788322934834707
>500	1.6603189155989246E-5	0.008214107968623038
>1k	3.319829486266798E-5	0.0412014014131432
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.654182085895007E-6	0.0	0.0	0.0
3	0.0	8.654182085895007E-6	0.0	0.0	0.0
4	0.0	4.327091042947504E-5	0.0	0.0	0.0
5	0.0	5.192509251537004E-5	0.0	0.0	0.0
6	8.654182085895007E-6	5.192509251537004E-5	0.0	0.0	0.0
7	8.654182085895007E-6	5.192509251537004E-5	0.0	0.0	0.0
8	8.654182085895007E-6	6.057927460126505E-5	0.0	0.0	8.654182085895007E-6
9	8.654182085895007E-6	1.5577527754611012E-4	0.0	2.596254625768502E-5	1.7308364171790015E-5
10-11	8.654182085895007E-6	1.5577527754611012E-4	0.0	3.0289637300632525E-5	1.7308364171790015E-5
12-13	8.654182085895007E-6	1.6442945963200513E-4	0.0	3.461672834358003E-5	1.7308364171790015E-5
14-15	1.7308364171790015E-5	2.293358252762177E-4	0.0	4.327091042947504E-5	5.192509251537004E-5
16-17	1.7308364171790015E-5	2.423170984050602E-4	0.0	4.327091042947504E-5	6.057927460126505E-5
18-19	1.7308364171790015E-5	2.6827964466274523E-4	0.0	4.327091042947504E-5	6.490636564421256E-5
20-21	1.7308364171790015E-5	2.9424219092043024E-4	0.0	6.057927460126505E-5	6.923345668716006E-5
22-23	1.7308364171790015E-5	3.331860103069578E-4	0.0	9.952309398779258E-5	6.923345668716006E-5
24-25	1.7308364171790015E-5	3.764569207364328E-4	0.0	1.4279400441726762E-4	6.923345668716006E-5
26-27	1.7308364171790015E-5	3.894381938652753E-4	0.0	2.466441894480077E-4	6.923345668716006E-5
28-29	1.7308364171790015E-5	3.980923759511703E-4	0.0	9.606142115343459E-4	6.923345668716006E-5
30-31	1.7308364171790015E-5	4.327091042947504E-4	0.0	0.003392439377670843	6.923345668716006E-5
32-33	2.596254625768502E-5	4.327091042947504E-4	0.0	0.007970501701109302	7.788763877305506E-5
34-35	2.596254625768502E-5	4.976154699389629E-4	0.0	0.01398948534184928	7.788763877305506E-5
36-37	2.596254625768502E-5	5.711760176690705E-4	0.0	0.02203354759068869	7.788763877305506E-5
38-39	2.596254625768502E-5	6.057927460126505E-4	0.0	0.03491097053450046	8.654182085895008E-5
40-41	2.596254625768502E-5	6.404094743562305E-4	0.0	0.04742059073966169	8.654182085895008E-5
42-43	2.596254625768502E-5	6.490636564421256E-4	0.0	0.05796138452028181	9.086891190189758E-5
44-45	2.596254625768502E-5	7.31278386258128E-4	0.0	0.06983059525108681	1.0385018503074008E-4
46-47	2.596254625768502E-5	8.091660250311831E-4	0.0	0.0819897210817693	1.125043671166351E-4
48-49	2.596254625768502E-5	8.134931160741307E-4	0.0	0.09368152107981345	1.125043671166351E-4
50-51	2.596254625768502E-5	9.086891190189757E-4	0.0	0.10523918125552623	1.125043671166351E-4
52-53	2.596254625768502E-5	9.130162100619233E-4	0.0	0.11605258177185204	1.2981273128842512E-4
54-55	2.596254625768502E-5	9.476329384055033E-4	0.0	0.12636403972719595	1.4712109546021512E-4
56-57	2.596254625768502E-5	9.519600294484508E-4	0.0	0.1360264340260977	1.4712109546021512E-4
58-59	2.596254625768502E-5	9.649413025772933E-4	0.0	0.14524746503861885	1.5144818650316262E-4
60-61	2.596254625768502E-5	9.779225757061358E-4	0.0	0.15461561714660021	1.5577527754611012E-4
62-63	3.461672834358003E-5	0.001003885121963821	0.0	0.16309671559077732	1.7308364171790016E-4
64-65	3.461672834358003E-5	0.001116389489080456	0.0	0.17135280530072114	1.7308364171790016E-4
66-67	3.461672834358003E-5	0.0011207165801234034	0.0	0.17960456791962204	1.8173782380379516E-4
68-69	3.461672834358003E-5	0.0011553333084669834	0.0	0.1882198061861305	1.9039200588969016E-4
70-71	3.461672834358003E-5	0.0011812958547246684	0.0	0.1961080931574238	1.9039200588969016E-4
72-73	3.461672834358003E-5	0.001185622945767616	0.0	0.2018804326087158	2.163545521473752E-4
74-75	3.461672834358003E-5	0.0011899500368105637	0.0	0.2064541678411113	2.163545521473752E-4
76-77	3.461672834358003E-5	0.001211585492025301	0.0	0.21070769833632869	2.206816431903227E-4
78-79	3.461672834358003E-5	0.001220239674111196	0.0	0.21433812772136165	2.250087342332702E-4
80-81	3.461672834358003E-5	0.0012245667651541435	0.0	0.2161381975952278	2.250087342332702E-4
82-83	3.461672834358003E-5	0.0012332209472400387	0.0	0.2167223548860257	2.250087342332702E-4
84-85	3.461672834358003E-5	0.0012375480382829861	0.0	0.2172113161738788	2.250087342332702E-4
86-87	3.461672834358003E-5	0.001246202220368881	0.0	0.21746228745436974	2.250087342332702E-4
88-89	3.461672834358003E-5	0.0012851460397554086	0.0	0.21756613763940047	2.250087342332702E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11610	0.0	42.40989	1
GTATCAA	19830	0.0	34.716015	1
ATCAACG	23655	0.0	28.1551	3
TCAACGC	23790	0.0	28.08243	4
CAACGCA	23960	0.0	27.796698	5
TATCAAC	25090	0.0	27.075132	2
AACGCAG	25060	0.0	26.692568	6
ACGCAGA	28885	0.0	23.014412	7
GTACATG	32590	0.0	22.662418	1
CGCAGAG	29410	0.0	22.560816	8
TACATGG	32260	0.0	22.30374	2
ACATGGG	32890	0.0	21.308058	3
CATGGGG	24180	0.0	19.625164	4
GCAGAGT	36285	0.0	18.285772	9
AGAGTAC	31315	0.0	17.211073	10-11
GAGTACT	18445	0.0	16.671486	12-13
CAGAGTA	35020	0.0	16.236334	10-11
GTGGTAT	6730	0.0	15.3344145	1
GTACTAG	2775	0.0	15.084861	1
ATGGGGG	15345	0.0	14.638497	5
>>END_MODULE